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公开(公告)号：US20220411739A1
公开(公告)日：2022-12-29
申请号：US17771255
申请日：2020-09-30
Applicant: Noile-Immune Biotech Inc. , SHIBUYA CORPORATION
Inventor： Kenji YONEDA , Naoya DEGUCHI , Noriaki NISHIMURA
Abstract: A genetically modified cell that can be mass-produced efficiently. The genetically modified cell production system includes a cell-processing isolator for seeding a T cell in a well plate (culture vessel) a cell culture incubator (cell storage) for holding the culture vessel in which the T cell has been seeded and culturing the T cell a virus-processing isolator (nucleic acid preparation isolator) for providing, to the well plate, a virus having a nucleic acid containing a gene to be introduced into a cell a virus storage (nucleic acid storage) for holding the well plate from the virus-processing isolator and a virus infection isolator (nucleic acid introduction isolator) for introducing the nucleic acid into the T cell by infecting the T cell with the virus.
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公开(公告)号：US20210062134A1
公开(公告)日：2021-03-04
申请号：US17001903
申请日：2020-08-25
Applicant: SHIBUYA CORPORATION
Inventor： Kenji YONEDA , Naoya DEGUCHI , Noriaki NISHIMURA
Abstract: To continue a series of cell treatments even in a case where a treatment has become impossible in any isolator, a cell treatment system is provided with at least two isolators that perform different treatments, such that a sequential series of cell treatments is performed in each of the isolators. The cell treatment system is provided with a first isolator that performs a first treatment, a second isolator that performs a second treatment different from the first treatment, and a common isolator capable of performing the first treatment and the second treatment.
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公开(公告)号：US20170088804A1
公开(公告)日：2017-03-30
申请号：US15280139
申请日：2016-09-29
Applicant: SHIBUYA CORPORATION
Inventor： Kenji YONEDA , Ichiro KOSHIDA
IPC: C12M1/12
Abstract: An apparatus 1 for producing a cell mass sheet is structured so as to produce a cell mass sheet 4 in which a plurality of cell masses 2 that have been planarly arranged on a mounting surface 13a in a culture container 3 are fused to each other by being cultured.The apparatus includes guide means G that has a plurality of accommodating portions Ga which can accommodate the cell masses 2 therein, and suction nozzles 8 (supply means) that supply the cell masses 2 into the accommodating portions Ga of the guide means G, which are arranged on the mounting surface 13a; and is structured so that the suction nozzles 8 array the cell masses 2 on the mounting surface 13a, and after that, the guide means G retracts from the above the mounting surface 13a. The apparatus can produce a cell mass sheet that has a constant shape and also a uniform thickness.
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公开(公告)号：US20160264921A1
公开(公告)日：2016-09-15
申请号：US15030179
申请日：2014-11-14
Applicant: SHIBUYA CORPORATION
Inventor： Kenji YONEDA
Abstract: It is constituted such that a sterile work can be applied by a worker 29 to cells or the like through a glove 28 provided in an isolator 15. A headset 31 for work outputting a work procedure of the sterile work in voice is prepared for the worker, and an input device 39 for check is prepared for a checker 36. A management device 12 outputs the work procedure of the sterile work this time in voice to the headset for work. When the fact that the worker has normally performed the sterile work this time is checked by the checker, and when the fact is input from the input device for check to the management device, the management device performs the subsequent work procedure.Since the sterile work by the worker 29 can be monitored by the checker 36, a wrong work can be prevented from being performed.
Abstract translation: 由于工人29的无菌工作可以由检验器36监视，因此可以防止执行错误的工作。
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Patent Agency Ranking
公开(公告)号：US20170121674A1
公开(公告)日：2017-05-04
申请号：US15337785
申请日：2016-10-28
Applicant: SHIBUYA CORPORATION
Inventor： Kenji YONEDA , Ichiro KOSHIDA , Akira HATANAKA , Hiroyuki NAGAI
CPC classification number: C12N5/0062 , C12M23/50 , C12M27/10 , C12M29/06 , C12M33/04 , C12M47/02 , C12N5/0068 , C12N2513/00 , C12N2535/10
Abstract: The present invention relates to a method for producing a cell mass structure, which produces a cell mass structure (adhesion pad 4) in which a plurality of cell aggregates 2 arranged inside a culture container 3 accommodating a culture liquid are fused to each other by being cultured. A plurality of pins 16 are erected inside the above-described culture container 3, the above-described cell aggregates 2 are accommodated by the plurality of pins 16 with parts between the plurality of pins 16 and pins 16 as accommodating portions H, and the cell aggregates 2 are cultured in a state of being accommodated in the plurality of adjacent accommodating portions H. It is possible to obtain a required shape and dimension, and the cell mass structure can be obtained without giving damage to cells further.
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公开(公告)号：US20220287922A1
公开(公告)日：2022-09-15
申请号：US17633405
申请日：2020-07-28
Inventor： Misako NAKASHIMA , Koichiro IOHARA , Kenji YONEDA , Ryosuke MURAI , Hiroyuki NAGAI
Abstract: A dentin regenerative cell culture that can bring about a rapid regeneration of dentin in a deficit region. In the treatment of a tooth using the dentin regenerative cell culture, a root canal where a pulpectomy has been performed is filled with a root canal filler containing dental pulp stem cells. The dentin regenerative cell culture is then implanted in the deficit region of dentin, and temporary sealing with a packing is carried out. The dentin regenerative cell culture is formed three-dimensionally in conformity with the shape of the deficit region with the coalescence of cell masses of a plurality of odontoblasts, and thus the dentin regeneration is well promoted. In addition, gaps between the dentin regenerative cell culture and biological tissue can be rapidly filled. Infection due to bacterial infiltration can thereby be prevented.
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公开(公告)号：US20190160108A1
公开(公告)日：2019-05-30
申请号：US16320763
申请日：2017-07-11
Applicant: YAMAGUCHI UNIVERSITY , SHIBUYA CORPORATION
Inventor： Isao SAKAIDA , Taro TAKAMI , Kenji YONEDA
Abstract: A bone marrow fluid of a patient is sampled, housed in a collection tube 11 and conveyed into an isolator in a sterile state. In the isolator, the bone marrow fluid in the collection tube is pipetted into test tubes and culture tube, etc. To the bone marrow fluid in the tubes, an erythrocyte sedimentation agent is added. After precipitating erythrocytes, the supernatant is collected. From the collected supernatant, a bone marrow cell-containing fraction for liver regeneration is concentrated and pipetted into flasks which are conveyed into an incubator to start the cell-culture. From the isolator system, test tubes are taken out. The bone marrow fluid in the test tubes and the concentrate are subjected to a safety test, etc.
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公开(公告)号：US20180240059A1
公开(公告)日：2018-08-23
申请号：US15550574
申请日：2016-01-22
Applicant: Shibuya Corporation
Inventor： Shiaru MURANAKA , Kenji YONEDA , Hiroshi UTSUMI
IPC: G06Q10/06 , G06Q50/04 , G05B19/418
Abstract: A schedule management system 1 having isolators 3 (production facilities) and a main control device 4 for managing a production schedules of all the isolators 3. The production process of each isolator constituting the production schedule has a plurality of sub-processes, the production status being determined in the sub-process.The main control device 4 adds a new separating operation to the passage process and performs an update when the main control device 4 obtains a determination result that the separation of cells in a separation state confirmation operation (sub-process) in the passage process is insufficient while a passage process for cells A is being carried out in the isolators.The main control device 4 alters the start time of a culture medium exchange process for other cells B different from the updated passage operation, and updates the production schedule.
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公开(公告)号：US20170246211A1
公开(公告)日：2017-08-31
申请号：US15512390
申请日：2015-08-19
Applicant: SHIBUYA CORPORATION , YAMAGUCHI UNIVERSITY
Inventor： Isao SAKAIDA , Shuji TERAI , Taro TAKAMI , Koichi FUJISAWA , Naoki YAMAMOTO , Kenji YONEDA
IPC: A61K35/28 , C12Q1/48 , C12N5/0775 , G01N33/50
Abstract: The present invention relates to a method for evaluating the activity level of mesenchymal stem cells, and a method for culturing mesenchymal stem cells using the evaluation method in the field of culturing mesenchymal stem cells for regenerative medicine, and further, a method for producing a therapeutic agent for liver dysfunction and a therapeutic agent for liver dysfunction. This method for evaluating mesenchymal stem cell activity according to the present invention comprises an assay step for assaying the amount of adenylate kinase 4 (AK4) in the mesenchymal stem cells; and a determination step for determining the activity level of the mesenchymal stem cells from the assayed amount of adenylate kinase 4.In this method, the activity level of mesenchymal stem cells can be evaluated from the assayed amount of AK4, for instance, if the mitochondrial activity level of mesenchymal stem cells is high, the mesenchymal stem cells can be evaluated as ideal for use as a therapeutic agent, while if mitochondrial activity of the mesenchymal stem cells is low and aging does not proceed, the mesenchymal stem cells can be evaluated as suitable for subculture.
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