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1.发明公开公开(公告)号：US20230203131A1
公开(公告)日：2023-06-29
申请号：US17923323
申请日：2021-05-07
Applicant: OSAKA UNIVERSITY , MATRIXOME, INC.
Inventor： Kiyotoshi SEKIGUCHI , Mamoru TAKIZAWA , Yukimasa TANIGUCHI
CPC classification number: C07K14/75 , C07K14/78 , C12N5/0068 , C12N5/0062 , C07K2319/00 , C12N2513/00
Abstract: The present invention provides a gel formed of fibrin and a molecule generated by thrombin treatment of a chimeric protein that comprises a fibrinogen fragment capable of binding to fibrinogen upon thrombin treatment and a laminin fragment having integrin-binding activity, and optionally further comprises a protein having growth factor-binding activity. The gel of the present invention is suitable as a gel substrate that has properties of the basement membrane and can be used in medical applications.
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公开(公告)号：US20160244721A1
公开(公告)日：2016-08-25
申请号：US15028158
申请日：2014-10-09
Applicant: HEALIOS K.K. , OSAKA UNIVERSITY
Inventor： Masanori SAWADA , Kiyotoshi SEKIGUCHI
CPC classification number: C12N5/0621 , A61K9/0048 , A61K35/30 , C12N2501/727 , C12N2506/45 , C12N2509/00 , C12N2533/52
Abstract: The present invention provides a method of purifying highly pure retinal pigment epithelial cells from a cell population obtained by induction of differentiation of pluripotent stem cells into retinal pigment epithelial cells, by a simple and easy operation in a short period. The purification method of the present invention includes a step of introducing a cell population containing retinal pigment epithelial cells obtained by differentiation induction of pluripotent stem cells on laminin or a fragment thereof on a filter, and obtaining a cell population that passed the filter.
Abstract translation: 本发明提供了通过在短时间内简单且容易的操作诱导将多能干细胞分化为视网膜色素上皮细胞而获得的细胞群中纯化高纯度视网膜色素上皮细胞的方法。 本发明的纯化方法包括将通过将多能干细胞分化诱导获得的视网膜色素上皮细胞的细胞群导入到过滤器上的层粘连蛋白或其片段上，获得通过过滤器的细胞群的步骤。
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公开(公告)号：US20170159020A1
公开(公告)日：2017-06-08
申请号：US15325743
申请日：2015-07-15
Applicant: OSAKA UNIVERSITY
Inventor： Kiyotoshi SEKIGUCHI , Ko TSUTSUI
IPC: C12N5/074
CPC classification number: C12N5/0696 , C12N15/00 , C12N15/09 , C12N2533/52
Abstract: The present invention provides a novel technique in a cell culture method using a cell culture vessel coated with a laminin fragment, which novel technique achieves cell culture as in the case of using a recommended coating concentration even when the coating concentration is lower than the recommended coating concentration. The present invention relates to a method for enhancing an activity for mammalian cultured cells of a laminin fragment or a variant thereof each having integrin binding activity.
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4.发明申请公开(公告)号：US20160052994A1
公开(公告)日：2016-02-25
申请号：US14758061
申请日：2013-11-11
Applicant: OSAKA UNIVERSITY
Inventor： Kiyotoshi SEKIGUCHI , Shaoliang LI , Ryoko SATO
CPC classification number: C07K14/78 , A61L27/227 , C07K2319/00 , C07K2319/02 , C07K2319/21 , C12N5/0696 , Y02P20/582
Abstract: A modified laminin characterized in that a laminin or a heterotrimeric laminin fragment has a collagen binding molecule conjugated to at least one site selected from the α chain N-terminus, the β chain N-terminus and the γ chain N-terminus, and an extracellular-matrix material comprising the modified laminin, and collagen and/or gelatin serve as an alternative to Matrigel and are useful as an extracellular-matrix material for the formation of a safe three-dimensional tissue structure for regenerative medicine in humans.
Abstract translation: 一种修饰的层粘连蛋白，其特征在于层粘连蛋白或异三聚体层粘连蛋白片段具有与至少一个选自α链N末端的位点结合的胶原结合分子。 链N末端和γ链N末端，以及包含修饰的层粘连蛋白的细胞外基质材料，胶原和/或明胶用作Matrigel的替代物，并且可用作形成安全的细胞外基质材料 人体再生医学的三维组织结构。
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公开(公告)号：US20220340868A1
公开(公告)日：2022-10-27
申请号：US17633082
申请日：2020-08-04
Applicant: KAO CORPORATION , OSAKA UNIVERSITY
Inventor： Yoriko NAKAGIRI , Kiyotoshi SEKIGUCHI
IPC: C12N5/071
Abstract: The present invention relates to improvement of the yield of skin-derived pluripotent precursor cells in induction of differentiation of stem cells to skin-derived pluripotent precursor cells. The present invention provides a method for preparing skin-derived pluripotent precursor cell comprising culturing a neural crest stem cells in the presence of at least one selected from the group consisting of laminin and a fragment thereof to differentiate the cells to skin-derived pluripotent precursor cells, wherein the laminin is at least one selected from the group consisting of laminin 111, laminin 121, laminin 332, laminin 421, laminin 511, laminin 521, and a variant thereof.
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Patent Agency Ranking
公开(公告)号：US20190153391A1
公开(公告)日：2019-05-23
申请号：US15511275
申请日：2015-09-14
Applicant: OSAKA UNIVERSITY
Inventor： Yukiko OCHI , Kiyotoshi SEKIGUCHI , Shigeru MIYAGAWA , Yoshiki SAWA , Antti Markus SILTANEN
CPC classification number: C12N5/0657 , A61K35/34 , C12N5/0696 , C12N2506/45 , C12N2533/52
Abstract: The present invention provides a method for preparing a clinically applicable, safe and less damaged cardiomyocyte population through a brief and simple procedure from a cell population obtained by induced differentiation of pluripotent stem cells into cardiomyocytes. The present invention relates to a method for preparing a cardiomyocyte population, the method comprising the steps of: (1) inducing pluripotent stem cells to differentiate into cardiomyocytes, (2) bringing a cell population obtained by the induced differentiation into contact with a laminin selected from the group consisting of laminin α2β1γ1, laminin α2β2γ1, laminin α1β1γ1 and laminin α1β2γ1, or a fragment thereof having integrin binding activity, and (3) retrieving cells adherent to the laminin or the laminin fragment.
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公开(公告)号：US20220298474A1
公开(公告)日：2022-09-22
申请号：US17633517
申请日：2020-08-06
Applicant: KAO CORPORATION , OSAKA UNIVERSITY
Inventor： Shiho YAMASHITA , Yoriko NAKAGIRI , Kiyotoshi SEKIGUCHI , Chisei SHIMONO
Abstract: Provided is a method for efficiently culturing dermal papilla cells while maintaining an ability to induce hair follicles. A method for culturing dermal papilla cells in the presence of at least one selected from the group consisting of EMILIN and a fragment thereof.
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公开(公告)号：US20160237403A1
公开(公告)日：2016-08-18
申请号：US15028076
申请日：2014-10-09
Applicant: HEALIOS K.K. , RIKEN , OSAKA UNIVERSITY
Inventor： Masanori SAWADA , Masayo TAKAHASHI , Kiyotoshi SEKIGUCHI
CPC classification number: C12N5/0621 , A61K9/0048 , A61K35/30 , C12N2501/727 , C12N2506/45 , C12N2509/00 , C12N2533/52
Abstract: Provided are a production method of retinal pigment epithelial (RPE) cells that improves differentiation induction efficiency of pluripotent stem cells into RPE cells, and can provide highly pure RPE cells by a simple and easy operation in a short period, a culture method of RPE cells that can stably grow and culture a cell, a toxicity/efficacy evaluation method using RPE cells useful for transplantation therapy, and a therapeutic drug for a retinal disease. The invention relates to a production method of RPE cells, comprising adhesion culture of human pluripotent stem cells using a culture substrate coated with a laminin-E8 fragment, a culture method of RPE cells, comprising adhesion culture of RPE cells using a culture substrate coated with a laminin-E8 fragment, a toxicity or efficacy evaluation method using RPE cells obtained by producing or culturing by the method, and a therapeutic drug for a retinal disease, containing the RPE cells.
Abstract translation: 提供了一种提高多能干细胞向RPE细胞分化诱导效率的视网膜色素上皮（RPE）细胞的制备方法，可以通过简单易用的操作在短时间内提供高纯度的RPE细胞，RPE细胞的培养方法 可以稳定地培养细胞，使用可用于移植治疗的RPE细胞的毒性/功效评价方法，以及用于视网膜疾病的治疗药物。 本发明涉及RPE细胞的制备方法，其包括使用包被有层粘连蛋白-E8片段的培养基底的人多能干细胞的粘附培养，RPE细胞的培养方法，包括使用涂覆有 层粘连蛋白E8片段，使用通过该方法生产或培养获得的RPE细胞的毒性或功效评价方法和含有RPE细胞的视网膜疾病治疗药。
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公开(公告)号：US20190276804A1
公开(公告)日：2019-09-12
申请号：US16348944
申请日：2017-11-09
Applicant: OSAKA UNIVERSITY , KYOTO UNIVERSITY
Inventor： Kiyotoshi SEKIGUCHI , Fumi EBISU , Hidetoshi SAKURAI , Mingming ZHAO , Megumu SAITO
Abstract: Provided is a method for inducing pluripotent stem cells to differentiate into somatic cells in a culture medium containing a heparin binding growth factor, the method comprising bringing cells into contact with a conjugate of a laminin E8 fragment and a growth factor binding domain-containing fragment of a heparan sulfate proteoglycan. According to the present invention, pluripotent stem cells can be induced to differentiate into any desired somatic cells in a highly efficient manner.
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公开(公告)号：US20160137965A1
公开(公告)日：2016-05-19
申请号：US14897298
申请日：2014-05-09
Applicant: OSAKA UNIVERSITY
Inventor： Kiyotoshi SEKIGUCHI , Ko TSUTSUI
IPC: C12M1/12 , C07K14/765 , C12N9/10 , C07K14/435 , C07K14/78 , C12N5/00 , C07K14/79
CPC classification number: C12M25/06 , C07K14/435 , C07K14/765 , C07K14/78 , C07K14/79 , C12M23/20 , C12N5/0068 , C12N9/1088 , C12N2533/00 , C12N2533/52 , C12Y205/01018
Abstract: Provided is a cell culture vessel characterized in that a surface to be in contact with cells is coated with a laminin fragment having integrin α6β1 binding activity or a modified form thereof in a dry state, the laminin fragment being derived from at least one kind selected from laminin α5β1γ1 and laminin α5β2γ1, the cell culture vessel being any of the following: (1) a cell culture vessel of which a surface to be in contact with cells is coated only with a laminin fragment having integrin α6β1 binding activity or a modified form thereof in a dry state; (2) a cell culture vessel of which a surface to be in contact with cells is coated with a laminin fragment having integrin α6β1 binding activity or a modified form thereof in combination with a laminin fragment having no integrin α6β1 binding activity in a dry state; and (3) a cell culture vessel of which a surface to be in contact with cells is coated with a laminin fragment having integrin α6β1 binding activity or a modified form thereof in combination with a protein that is neither a laminin nor a laminin fragment, in a dry state.
Abstract translation: 本发明提供一种细胞培养容器，其特征在于，在干燥状态下，与细胞接触的表面涂布有具有整合素α6和bgr1结合活性的层粘连蛋白片段或其修饰形式，所述层粘连蛋白片段衍生自至少一种 选自层粘连蛋白α5＆bgr;1γ1和层粘连蛋白α5和bgr;2γ1，细胞培养容器为以下任一种：（1）细胞培养容器，其与细胞接触的表面仅具有整合素α6和bgr的层粘连蛋白片段; 1结合活性或其干燥状态的修饰形式; （2）将与细胞接触的表面的细胞培养容器与具有整合素α6和bgr1结合活性的层粘连蛋白片段或其修饰形式与不具有整合素α6和bgr1结合活性的层粘连蛋白片段组合 干燥状态 和（3）细胞培养容器，其与细胞接触的表面用具有整合素α6和bgr1结合活性的层粘连蛋白片段或其修饰形式与既不是层粘连蛋白也不是层粘连蛋白片段的蛋白质组合 ，处于干燥状态。
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