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1.发明授权公开(公告)号：US07288403B2
公开(公告)日：2007-10-30
申请号：US11196176
申请日：2005-08-03
CPC分类号： C12N15/8241 , C12N9/0006 , C12N9/88 , C12N15/8207 , C12N15/8209 , C12N15/8216 , C12N15/8251 , C12N15/8254
摘要： The present invention provides a method for conferring tolerance to an amino acid analog of tryptophan to a plant and/or altering the tryptophan content of a plant by introducing and expressing an isolated DNA segment encoding an anthranilate synthase in the cells of the plant. Transgenic plants transformed with an isolated DNA segment encoding an anthranilate synthase, as well as seeds and progeny derived from these plants, are also provided. The present invention also provides a cDNA sequence of an alpha and a beta subunit of a maize anthranilate synthase.
摘要翻译： 本发明提供了通过在植物细胞中引入和表达编码邻氨基苯甲酸合酶的分离的DNA区段来赋予植物对色氨酸的氨基酸类似物的耐受性和/或改变植物的色氨酸含量的方法。 还提供了用编码邻氨基苯甲酸合酶的分离的DNA区转化的转基因植物，以及源自这些植物的种子和后代。 本发明还提供了玉米邻氨基苯甲酸合酶的α和β亚基的cDNA序列。
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2.发明授权公开(公告)号：US06515201B2
公开(公告)日：2003-02-04
申请号：US09733300
申请日：2000-12-08
IPC分类号： A01H100
CPC分类号： C12N15/8241 , C12N9/0006 , C12N9/88 , C12N15/8207 , C12N15/8209 , C12N15/8216 , C12N15/8251 , C12N15/8254
摘要： The present invention provides a method for conferring tolerance to an amino acid analog of tryptophan to a plant and/or altering the tryptophan content of a plant by introducing and expressing an isolated DNA segment encoding an anthranilate synthase in the cells of the plant. Transgenic plants transformed with an isolated DNA segment encoding an anthranilate synthase, as well as seeds and progeny derived from these plants, are also provided. The present invention also provides a cDNA sequence of an alpha and a beta subunit of a maize anthranilate synthase.
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3.发明授权
公开(公告)号：US6118047A
公开(公告)日：2000-09-12
申请号：US604789
申请日：1996-01-19
IPC分类号： A01H5/00 , C12N5/10 , C12N9/04 , C12N9/88 , C12N15/09 , C12N15/29 , C12N15/60 , C12N15/82 , C12R1/91 , C12N5/04
CPC分类号： C12N15/8241 , C12N15/8207 , C12N15/8209 , C12N15/8216 , C12N15/8251 , C12N15/8254 , C12N9/0006 , C12N9/88
摘要： The present invention provides a method for conferring tolerance to an amino acid analog of tryptophan to a plant and/or altering the tryptophan content of a plant by introducing and expressing an isolated DNA segment encoding an anthranilate synthase in the cells of the plant. Transgenic plants transformed with an isolated DNA segment encoding an anthranilate synthase, as well as seeds and progeny derived from these plants, are also provided. The present invention also provides a cDNA sequence of an alpha and a beta subunit of a maize anthranilate synthase.
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4.发明授权Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction 有权邻氨基苯甲酸合酶基因及其用于赋予色氨酸过量产生的方法公开(公告)号：US06271016B1
公开(公告)日：2001-08-07
申请号：US09312721
申请日：1999-05-17
IPC分类号： C12N988
CPC分类号： C12N15/8241 , C12N9/0006 , C12N9/88 , C12N15/8207 , C12N15/8209 , C12N15/8216 , C12N15/8251 , C12N15/8254
摘要： The present invention provides a method for conferring tolerance to an amino acid analog of tryptophan to a plant and/or altering the tryptophan content of a plant by introducing and expressing an isolated DNA segment encoding an anthranilate synthase in the cells of the plant. Transgenic plants transformed with an isolated DNA segment encoding an anthranilate synthase, as well as seeds and progeny derived from these plants, are also provided. The present invention also provides a cDNA sequence of an alpha and a beta subunit of a maize anthranilate synthase.
摘要翻译： 本发明提供了通过在植物细胞中引入和表达编码邻氨基苯甲酸合酶的分离的DNA区段来赋予植物对色氨酸的氨基酸类似物的耐受性和/或改变植物的色氨酸含量的方法。 还提供了用编码邻氨基苯甲酸合酶的分离的DNA区转化的转基因植物，以及源自这些植物的种子和后代。 本发明还提供了玉米邻氨基苯甲酸合酶的α和β亚基的cDNA序列。
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5.发明授权Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof 失效用于生产稳定转化的肥沃单子叶植物及其细胞的方法和组合物公开(公告)号：US07705215B1
公开(公告)日：2010-04-27
申请号：US08113561
申请日：1993-08-25
申请人： Thomas R. Adams , Paul C. Anderson , Sheryl A. Chambers , Paul S. Chomet , Richard J. Daines , Christopher E. Flick , Kimberly Glassman , William J. Gordon-Kamm , Albert P. Kausch , Lucille B. Laccetti , Peggy G. Lemaux , Catherine J. Mackey , Mary Lou Mangano , Michael T. Mann , James V. O'Brien , Emil M. Orozco , Peter Orr , Thomas B. Rice , T. Michael Spencer , William G. Start , Michael A. Stephens , Clayton S. Vetsch , David A. Walters , Donald S. Walters , Nancy G. Willetts , Susan J. Zachwieja
发明人： Thomas R. Adams , Paul C. Anderson , Sheryl A. Chambers , Paul S. Chomet , Richard J. Daines , Christopher E. Flick , Kimberly Glassman , William J. Gordon-Kamm , Albert P. Kausch , Lucille B. Laccetti , Peggy G. Lemaux , Catherine J. Mackey , Mary Lou Mangano , Michael T. Mann , James V. O'Brien , Emil M. Orozco , Peter Orr , Thomas B. Rice , T. Michael Spencer , William G. Start , Michael A. Stephens , Clayton S. Vetsch , David A. Walters , Donald S. Walters , Nancy G. Willetts , Susan J. Zachwieja
CPC分类号： C12N15/8241 , A01H4/00 , A01H4/001 , C07K14/415 , C12N5/0025 , C12N5/04 , C12N9/0006 , C12N9/88 , C12N15/8207 , C12N15/8209 , C12N15/87
摘要： This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.
摘要翻译： 本发明涉及用于生产稳定的遗传转化的玉米细胞的可再现系统，以及选择已经转化的细胞的方法。 公开的一种选择方法使用通过微粒轰击引入的链霉菌棒基因，其在悬浮培养物中生长，然后暴露于除草剂双乙二胺磷的胚发生玉米细胞中。 实现本文公开的稳定转化的方法包括组织培养方法和培养基，用所需转化DNA轰击受体细胞的方法，以及从转化细胞生长可育植物的方法。 本发明还涉及转化的细胞和种子以及从转化细胞和其花粉生长的可育植物。
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公开(公告)号：US09121028B2
公开(公告)日：2015-09-01
申请号：US11518571
申请日：2006-09-08
IPC分类号： C12N15/113 , C12N15/82
CPC分类号： C12N15/8216 , C12N15/8218
摘要： This invention discloses methods for providing plants with selective expression of a target RNA and methods for evaluating an expression pattern of a target RNA in a plant. Also disclosed are transgenic plants having recombinant DNA for expressing a protein using a promoter functional in multiple tissues, and recombinant DNA for suppressing expression of the protein using a promoter functional in fewer than the multiple tissues.
摘要翻译： 本发明公开了提供植物选择性表达靶RNA的方法和用于评估植物中靶RNA表达模式的方法。 还公开了具有使用在多个组织中起作用的启动子表达蛋白质的重组DNA的转基因植物，以及使用在少于多个组织中功能的启动子抑制蛋白质表达的重组DNA。
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公开(公告)号：US08829275B2
公开(公告)日：2014-09-09
申请号：US13558336
申请日：2012-07-25
申请人： Xudong Ye , Michael W. Petersen , Larry Gilbertson , David Walters , Susan Johnson , Shihshieh Huang , Paul S. Chomet
发明人： Xudong Ye , Michael W. Petersen , Larry Gilbertson , David Walters , Susan Johnson , Shihshieh Huang , Paul S. Chomet
IPC分类号： C12N15/113 , C12N15/82 , C12N15/84 , C12N5/10 , A01H1/04 , A01H1/02 , A01H5/00 , C12N9/10 , C12Q1/68
CPC分类号： C12Q1/6895 , A01H1/04 , C12N9/1085 , C12N15/8205 , C12N15/821 , C12N15/8212 , C12N15/8218 , C12N15/8234 , C12N15/8245 , C12N15/8247 , C12N15/8261 , C12N15/8263 , C12N15/8265 , C12N15/8274 , C12N15/8279 , C12N15/8286 , C12Q2600/13 , C12Q2600/156
摘要： The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.
摘要翻译： 本发明提供用于鉴定含有感兴趣的转基因但缺少标记基因的转基因种子的方法和组合物。 使用导致可检测表型的鉴定序列增加筛选未与目的基因连接的转基因序列与编码感兴趣的基因的序列分离的种子和植物的效率。
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公开(公告)号：US20120227131A1
公开(公告)日：2012-09-06
申请号：US13199773
申请日：2011-09-08
申请人： Mark S. Abad , Thomas R. Adams , Ameeta K. Argarwal , James A. Ball , Paolo Castiglioni , Banu Gopalan , Erin Bell , Shobha Char , Robert J. Bensen , Raghava S. Boddupalli , Alessandra Frizzi , Jill Deikman , Robert D'Ordine , Michael D. Edgerton , Xianfeng Chen , Mary Fernandes , Paul S. Chomet , Stephen Duff , Deborah G. Rucker , Changlin Fu , Jeffrey E. Ahrens , Stanton B. Dotson , Richard E. Finger , Jinzhuo Dong , Molian Deng
发明人： Mark S. Abad , Thomas R. Adams , Ameeta K. Argarwal , James A. Ball , Paolo Castiglioni , Banu Gopalan , Erin Bell , Shobha Char , Robert J. Bensen , Raghava S. Boddupalli , Alessandra Frizzi , Jill Deikman , Robert D'Ordine , Michael D. Edgerton , Xianfeng Chen , Mary Fernandes , Paul S. Chomet , Stephen Duff , Deborah G. Rucker , Changlin Fu , Jeffrey E. Ahrens , Stanton B. Dotson , Richard E. Finger , Jinzhuo Dong , Molian Deng
CPC分类号： C12N15/8209 , A01H5/10 , C07K14/4702 , C12N15/8247 , C12N15/8251 , C12N15/8261 , C12N15/8273 , C12N15/8274
摘要： This invention provides transgenic plant cells with recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits.
摘要翻译： 本发明提供具有用于表达蛋白质的重组DNA的转基因植物细胞，其可用于赋予转基因作物植物增强的农艺性状。 本发明还提供包含转基因植物细胞的转基因植物和后代种子，其中植物被选择用于具有增强的性状，所述性状选自由增强的水利用效率，增强的耐寒性，增加的产量，增强的氮利用效率，增强的氮利用效率， 种子蛋白和增强种子油。 还公开了用于制备具有增强特征的转基因种子和植物的方法。
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公开(公告)号：US20120131699A1
公开(公告)日：2012-05-24
申请号：US13271666
申请日：2011-10-12
申请人： Xudong Ye , Michael W. Petersen , Larry Gilbertson , David Walters , Susan Johnson , Shihshieh Huang , Paul S. Chomet
发明人： Xudong Ye , Michael W. Petersen , Larry Gilbertson , David Walters , Susan Johnson , Shihshieh Huang , Paul S. Chomet
CPC分类号： C12Q1/6895 , A01H1/04 , C12N9/1085 , C12N15/8205 , C12N15/821 , C12N15/8212 , C12N15/8218 , C12N15/8234 , C12N15/8245 , C12N15/8247 , C12N15/8261 , C12N15/8263 , C12N15/8265 , C12N15/8274 , C12N15/8279 , C12N15/8286 , C12Q2600/13 , C12Q2600/156
摘要： The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.
摘要翻译： 本发明提供用于鉴定含有感兴趣的转基因但缺少标记基因的转基因种子的方法和组合物。 使用导致可检测表型的鉴定序列增加筛选未与目的基因连接的转基因序列与编码感兴趣的基因的序列分离的种子和植物的效率。
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公开(公告)号：US06569648B1
公开(公告)日：2003-05-27
申请号：US09357711
申请日：1999-07-20
IPC分类号： C12P1934
CPC分类号： C12Q1/6895 , C12Q1/6837
摘要： The present invention provides a highly-efficient method for the selection and identification of insertional mutants. In the technique, a non-selective amplification is used to isolate a plurality of insertion events from a population of individuals comprising insertion mutations. Specific insertion events can then be identified from the population by the use of gene specific probes or primers. Through the identification of mutants for a particular gene, data may be obtained regarding the function and phenotypic effects of that gene, and thereby, the gene can be employed in the creation of novel biotechnological products.
摘要翻译： 本发明提供了一种用于选择和鉴定插入突变体的高效方法。 在该技术中，非选择性扩增用于从包含插入突变的个体群体中分离多个插入事件。 然后可以通过使用基因特异性探针或引物从群体中鉴定特异性插入事件。 通过鉴定特定基因的突变体，可以获得关于该基因的功能和表型效应的数据，从而可以将该基因用于创建新的生物技术产品。
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