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公开(公告)号：US20060246121A1
公开(公告)日：2006-11-02
申请号：US11410480
申请日：2006-04-25
申请人： Peter Ma , Guobao Wei
发明人： Peter Ma , Guobao Wei
CPC分类号： A61K9/0024 , A61K9/1647 , A61K9/1652 , A61K9/1658 , A61K9/1694 , A61K9/5153 , A61K9/5161 , A61K9/5169 , A61K9/70 , A61K48/0008 , A61L27/54 , A61L27/56 , A61L2300/252 , A61L2300/258 , A61L2300/43 , A61L2300/602 , A61L2300/622 , A61L2300/624
摘要： Porous materials and methods for forming them are disclosed. One method for immobilizing micro-particles and/or nano-particles onto internal pore surfaces and/or external pore surfaces of porous materials includes suspending the micro-particles and/or nano-particles in a liquid adapted to swell, soften, and/or deform either the porous materials and/or the particles, thereby forming a liquid-particle suspension. The method further includes adding the suspension to the porous materials; and removing the liquid, thereby forming the porous materials having the micro-particles and/or nano-particles immobilized on the internal pore surfaces and/or the external pore surfaces.
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公开(公告)号：US20060083041A1
公开(公告)日：2006-04-20
申请号：US11269659
申请日：2005-11-09
申请人： Stanley Ma , Peter Ma , Valerie Lines , Peter Gillingham , Robert McKenzie , Abdullah Ahmed
发明人： Stanley Ma , Peter Ma , Valerie Lines , Peter Gillingham , Robert McKenzie , Abdullah Ahmed
IPC分类号： G11C15/00
CPC分类号： G11C7/06 , G11C15/04 , G11C15/043
摘要： A matchline sense circuit for detecting a rising voltage on a matchline of a CAM array is disclosed. The circuit initially precharges a matchline to ground before turning on a current source to supply current to the matchline and raise the voltage of the matchline. A reference matchline sense circuit generates a self-timed control signal to keep the current supply turned on for a predetermined duration of time. Sensed data on the matchlines are latched after the current source is turned off and the matchlines are precharged to ground. Because the matchline sense circuit of the present invention precharges the matchlines to ground instead of the supply voltage, VDD, less power is consumed. By sensing the rise of the matchline voltage to an n-channel transistor threshold potential, the matchline sensing operation speed is increased.
摘要翻译： 公开了一种用于检测CAM阵列的匹配线上的上升电压的匹配线检测电路。 在开启电流源以将电流提供给匹配线之前，该电路首先将匹配线预先接地，并提高匹配线的电压。 参考匹配线检测电路产生自定时控制信号以保持电流供应在预定持续时间内接通。 电流源关闭后，匹配线上的感测数据将被锁存，并将匹配线预充电到地。 因为本发明的匹配线检测电路将匹配线预充电到地电而不是电源电压VDD，所以消耗较少的功率。 通过感测匹配线电压升高到n沟道晶体管阈值电位，匹配线感测操作速度增加。
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公开(公告)号：US20120135414A1
公开(公告)日：2012-05-31
申请号：US13284839
申请日：2011-10-28
申请人： Linda LEE , Sam Lee Woo , Peter Ma
发明人： Linda LEE , Sam Lee Woo , Peter Ma
CPC分类号： C12Q1/686 , C12Q1/6853 , C12Q1/6869 , C12Q1/6874 , C12Q2525/113 , C12Q2525/125
摘要： A composition is provided comprising a negatively charged group, an oligonucleotide sequence and at least none or one nuclease-resistant linkage group to form a chemically-enhanced primer. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the primer as well as a method of preparing DNA for sequencing and a method of sequencing DNA and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition under conditions in which excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.
摘要翻译： 提供了一种组合物，其包含带负电荷的基团，寡核苷酸序列和至少一个或一个核酸酶抗性连锁基团以形成化学增强的引物。 化学增强引物可用于测序和片段分析。 还提供了合成引物的方法以及用于测序的DNA的制备方法和测序DNA的方法以及含有化学增强的引物的试剂盒。 DNA测序的方法可以包括使扩增反应产物与组合物在过量扩增引物被核酸酶降解的条件下接触，化学增强引物基本上不降解。
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公开(公告)号：US20070258277A1
公开(公告)日：2007-11-08
申请号：US11774881
申请日：2007-07-09
申请人： Stanley Ma , Peter Ma , Valerie Lines , Peter Gillingham , Robert McKenzie , Abdullah Ahmed
发明人： Stanley Ma , Peter Ma , Valerie Lines , Peter Gillingham , Robert McKenzie , Abdullah Ahmed
IPC分类号： G11C15/00
CPC分类号： G11C7/06 , G11C15/04 , G11C15/043
摘要： A matchline sense circuit for detecting a rising voltage on a matchline of a CAM array is disclosed. The circuit initially precharges a matchline to ground before turning on a current source to supply current to the matchline and raise the voltage of the matchline. A reference matchline sense circuit generates a self-timed control signal to keep the current supply turned on for a predetermined duration of time. Sensed data on the matchlines are latched after the current source is turned off and the matchlines are precharged to ground. Because the matchline sense circuit of the present invention precharges the matchlines to ground instead of the supply voltage, VDD, less power is consumed. By sensing the rise of the matchline voltage to an n-channel transistor threshold potential, the matchline sensing operation speed is increased.
摘要翻译： 公开了一种用于检测CAM阵列的匹配线上的上升电压的匹配线检测电路。 在开启电流源以将电流提供给匹配线之前，该电路首先将匹配线预先接地，并提高匹配线的电压。 参考匹配线检测电路产生自定时控制信号以保持电流供应在预定持续时间内接通。 电流源关闭后，匹配线上的感测数据将被锁存，并将匹配线预充电到地。 因为本发明的匹配线检测电路将匹配线预充电到地电而不是电源电压VDD，所以消耗较少的功率。 通过感测匹配线电压升高到n沟道晶体管阈值电位，匹配线感测操作速度增加。
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公开(公告)号：US20070036844A1
公开(公告)日：2007-02-15
申请号：US11496238
申请日：2006-07-31
申请人： Peter Ma , Victor Chen
发明人： Peter Ma , Victor Chen
摘要： A method for forming a porous three-dimensional (3-D) object includes creating a mold from a negative replica of the 3-D object, the 3-D object having a first size and at least one predetermined feature, and then casting a flowable material into and/or onto the mold. The method further includes forming pores of a second size and/or a third size in the flowable material, thereby forming the porous 3-D object.
摘要翻译： 用于形成多孔三维（3-D）物体的方法包括从3-D物体的阴性复制品形成模具，该3D物体具有第一尺寸和至少一个预定特征，然后浇铸 可流动的材料进入模具和/或模具上。 该方法还包括在可流动材料中形成第二尺寸和/或第三尺寸的孔，从而形成多孔3-D物体。
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公开(公告)号：US20060141487A1
公开(公告)日：2006-06-29
申请号：US11044236
申请日：2005-01-26
申请人： Peter Ma
发明人： Peter Ma
CPC分类号： C12Q1/6869 , C12Q2535/101 , C12Q2547/101 , C12Q2527/101
摘要： In one aspect, there are provided methods of amplifying and sequencing a polynucleotide. In some embodiments, the method includes (a) amplifying the polynucleotide with at least one amplification primer, a processive amplification polymerase, a sequencing primer, a sequencing polymerase, deoxynucleoside triphosphates suitable for template-dependent primer extension, and one or more terminating nucleotides, the incubation being carried out at a first temperature suitable for amplifying the polynucleotide with the processive amplification polymerase; (b) incubating the product of step (a) at a second temperature suitable for forming a plurality of differently-sized extended sequencing primers with the sequencing polymerase; (c) evaluating the extended sequencing primers in order to determine the sequence of the polynucleotide. The reactions at the first and second temperatures can be carried out in a single reaction vessel. In other aspects, compositions and kits for carrying out the methods are also provided.
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公开(公告)号：US06454796B1
公开(公告)日：2002-09-24
申请号：US09566234
申请日：2000-05-05
申请人： Kimberley Barkman , Peter Ma , Richard S. Williams
发明人： Kimberley Barkman , Peter Ma , Richard S. Williams
IPC分类号： A61F206
CPC分类号： A61F2/07 , A61F2/848 , A61F2/89 , A61F2002/065 , A61F2002/075 , A61F2002/8486 , A61F2220/0058 , A61F2220/0075 , A61F2230/0054 , A61F2250/0037 , A61F2250/0039
摘要： A bifurcated vascular graft having a tubular trunk with an inner diameter. Tubular limbs having respective inner diameters extend longitudinally from the trunk, and the diameter of at least one of the limbs is unequal to half the trunk diameter. In one embodiment, the graft is flared such that the combined diameters of the limbs exceed the diameter of the trunk. In another embodiment, the graft is tapered such that the diameter of the trunk exceeds the combined diameters of the limbs. In a third embodiment, the limb diameters are unequal to one another.
摘要翻译： 具有内径的管状躯干的分叉血管移植物。 具有相应内径的管状肢体从躯干纵向延伸，并且至少一个肢体的直径不等于躯干直径的一半。 在一个实施例中，移植物展开，使得四肢的组合直径超过躯干的直径。 在另一个实施例中，移植物是锥形的，使得躯干的直径超过四肢的组合直径。 在第三实施例中，肢体直径彼此不相等。
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公开(公告)号：US08703925B2
公开(公告)日：2014-04-22
申请号：US13397626
申请日：2012-02-15
CPC分类号： C12Q1/686 , C12Q1/6869 , C12Q1/6874 , C12Q2525/113 , C12Q2525/125
摘要： A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.
摘要翻译： 提供化学增强的引物，其包含带负电荷的部分（NCM），具有a）非核酸酶抗性核苷酸间连接的寡核苷酸序列或b）至少一个核酸酶抗性核苷酸间连接。 化学增强引物可用于测序和片段分析。 还提供了用于合成化学增强引物的方法以及制备用于测序的DNA的方法，测序DNA的方法和含有化学增强的引物的试剂盒。 测序DNA的方法可以包括使扩增反应产物与其中过量扩增引物被核酸酶降解并且化学增强引物基本上未降解的组合物接触。
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9.发明申请Methods, Compositions, and Kits for Amplifying and Sequencing Polynucleotides 有权用于扩增和测序多核苷酸的方法，组合物和试剂盒公开(公告)号：US20110070580A1
公开(公告)日：2011-03-24
申请号：US12684089
申请日：2010-01-07
申请人： Peter Ma
发明人： Peter Ma
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q2535/101 , C12Q2547/101 , C12Q2527/101
摘要： In one aspect, there are provided methods of amplifying and sequencing a polynucleotide. In some embodiments, the method includes (a) amplifying the polynucleotide with at least one amplification primer, a processive amplification polymerase, a sequencing primer, a sequencing polymerase, deoxynucleoside triphosphates suitable for template-dependent primer extension, and one or more terminating nucleotides, the incubation being carried out at a first temperature suitable for amplifying the polynucleotide with the processive amplification polymerase; (b) incubating the product of step (a) at a second temperature suitable for forming a plurality of differently-sized extended sequencing primers with the sequencing polymerase; (c) evaluating the extended sequencing primers in order to determine the sequence of the polynucleotide. The reactions at the first and second temperatures can be carried out in a single reaction vessel. In other aspects, compositions and kits for carrying out the methods are also provided.
摘要翻译： 一方面，提供了扩增和测序多核苷酸的方法。 在一些实施方案中，所述方法包括（a）用至少一个扩增引物，进程扩增聚合酶，测序引物，测序聚合酶，适于模板依赖性引物延伸的脱氧核苷三磷酸和一个或多个终止核苷酸扩增多核苷酸， 孵育在适于用进行性扩增聚合酶扩增多核苷酸的第一温度下进行; （b）在适于形成多个不同大小的扩增测序引物的第二温度下用所述测序聚合酶孵育步骤（a）的产物; （c）评估扩增的测序引物以确定多核苷酸的序列。 在第一和第二温度下的反应可以在单个反应容器中进行。 在其它方面，还提供了用于实施方法的组合物和试剂盒。
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公开(公告)号：US20060115644A1
公开(公告)日：2006-06-01
申请号：US10999459
申请日：2004-11-30
申请人： Peter Ma , Xiaohua Liu
发明人： Peter Ma , Xiaohua Liu
IPC分类号： B32B27/00
CPC分类号： C08J9/36 , Y10T428/249967 , Y10T428/249991 , Y10T428/249992 , Y10T428/249993
摘要： Modified porous materials are disclosed having interconnected, complexly shaped three-dimensional surfaces. The modification is accomplished by crosslinking the three-dimensional surfaces and/or by coating the three-dimensional surfaces with a layer of a predetermined material. The porous materials are macro structures including at least one of nano-features, micro-features, and combinations thereof. The modifying accomplishes changing surface properties of the porous materials, changing the three-dimensional surfaces, and/or rendering the porous materials substantially stable in a predetermined environment.
摘要翻译： 公开了具有互连的，复杂形状的三维表面的改性多孔材料。 通过使三维表面交联和/或通过用预定材料层涂覆三维表面来实现改性。 多孔材料是包括纳米特征，微特征及其组合中的至少一个的宏观结构。 修饰完成了改变多孔材料的表面性质，改变三维表面和/或使多孔材料在预定环境中基本稳定。
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