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11 条记录 (耗时 0.025 秒)

    Efficient drug screening for protein targets
    2.
    发明申请
    Efficient drug screening for protein targets 审中-公开
    蛋白质靶标的高效药物筛选

    公开(公告)号：US20070218475A1

    公开(公告)日：2007-09-20

    申请号：US11603473

    申请日：2006-11-21

    申请人： Philip Beachy ,  Anssi Taipale ,  James Chen

    发明人： Philip Beachy ,  Anssi Taipale ,  James Chen

    IPC分类号： C40B30/06 ,  C40B40/08 ,  C40B40/10

    CPC分类号： G01N33/5008 ,  G01N33/5023 ,  G01N33/5041

    摘要： The methods of the invention allow extremely high throughput screening of chemicals against a large number of pharmacologically relevant targets. All of the pharmacologically relevant targets are expressed in cells, which are then screened against a fluorescently-tagged combinatorial library. Binding of the small molecules to the cells is then detected by fluorescence activated cell sorting or imaging.

    摘要翻译： 本发明的方法允许针对大量药理学相关靶标的化学品的极高通量筛选。 所有与药物相关的靶标均在细胞中表达，然后将其与荧光标记的组合文库进行筛选。 然后通过荧光激活细胞分选或成像检测小分子与细胞的结合。

    Neuronal cell lineages and methods of production thereof
    5.
    发明申请
    Neuronal cell lineages and methods of production thereof 审中-公开
    神经元细胞谱系及其生产方法

    公开(公告)号：US20070053883A1

    公开(公告)日：2007-03-08

    申请号：US10550220

    申请日：2004-03-24

    申请人： Philip Beachy ,  Michael Cooper

    发明人： Philip Beachy ,  Michael Cooper

    IPC分类号： A61K35/30 ,  A61K38/17 ,  A61K31/724 ,  C12N5/08

    CPC分类号： A61K35/30 ,  C12N5/0619 ,  C12N2500/36 ,  C12N2501/41 ,  C12N2501/90

    摘要： Methods are provided in which a sterol-depleting agent such as β-cyclodextrin (βCD), is used to modulate signaling by a differentiation signaling protein such as Hedgehog protein or a BMP in vitro. As illustrated herein, the use of βCD in conjunction with Sonic Hedgehog N-terminal peptide (ShhN) causes substantially all of the cells in neural plate explants to differentiate as neurons, such as motor neurons. Also provided are cell therapy methods and screening methods that rely on the modulation of signaling of a differentiation signaling protein such as Hedgehog, by a sterol-depleting agent such as βCD.

    摘要翻译： 提供了其中使用甾体消耗剂如β-环糊精（βCD）来调节由分化信号蛋白如Hedgehog蛋白或BMP在体外的信号转导的方法。 如本文所示，βCD与Sonic Hedgehog N末端肽（ShhN）结合使用导致神经平板外植体中的所有细胞基本上分化为神经元，例如运动神经元。 还提供了依赖于诸如Hedgehog的分化信号蛋白的信号调节的细胞治疗方法和筛选方法，其通过消耗甾烷醇的消耗剂如βCD。

    Methods of predicting behavior of cancers
    6.
    发明申请
    Methods of predicting behavior of cancers 审中-公开
    预测癌症行为的方法

    公开(公告)号：US20070231828A1

    公开(公告)日：2007-10-04

    申请号：US11594281

    申请日：2006-11-07

    申请人： Philip Beachy ,  David Berman ,  Sunil Karhadkar ,  Edward Schaeffer ,  Angelo DeMarzo

    发明人： Philip Beachy ,  David Berman ,  Sunil Karhadkar ,  Edward Schaeffer ,  Angelo DeMarzo

    IPC分类号： C12Q1/02 ,  G01N33/53

    CPC分类号： G01N33/57434

    摘要： Elevated Hedgehog (Hh) pathway activity, including ligand stimulated Hh pathway activity, was detected in prostate tumors, and determined to be associated with growth and proliferation of the cancer cells. Accordingly, methods are provided for treating a prostate cancer associated with elevated Hh pathway activity by reducing or inhibiting the Hh pathway activity. Also provided are methods of identifying a prostate tumor of a subject as, or as capable of becoming lethal and/or metastatic.

    摘要翻译： 在前列腺肿瘤中检测到Hedge通路活性升高（包括配体刺激的Hh通路活性），并确定与癌细胞的生长和增殖相关。 因此，提供了通过降低或抑制Hh通路活性来治疗与升高的Hh通路活性相关的前列腺癌的方法。 还提供了鉴定受试者的前列腺肿瘤或能够变成致死和/或转移的方法。