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    Porous substrates for DNA arrays
    1.
    发明授权
    Porous substrates for DNA arrays 失效
    用于DNA阵列的多孔底物

    公开(公告)号：US06994972B2

    公开(公告)日：2006-02-07

    申请号：US10101144

    申请日：2002-03-18

    申请人： Pronob Bardhan ,  Dana C. Bookbinder ,  Joydeep Lahiri ,  Cameron W. Tanner ,  Patrick D. Tepesch ,  Raja R. Wusirika

    发明人： Pronob Bardhan ,  Dana C. Bookbinder ,  Joydeep Lahiri ,  Cameron W. Tanner ,  Patrick D. Tepesch ,  Raja R. Wusirika

    IPC分类号： C12Q1/68 ,  C12M1/36 ,  G07N15/06 ,  C07H21/04

    CPC分类号： C03C11/005 ,  B01J20/28033 ,  B01J20/28057 ,  B01J20/28083 ,  B01J20/28097 ,  B01J2219/00529 ,  B01J2219/00533 ,  B01J2219/00576 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00722 ,  B82Y30/00 ,  C03C17/30 ,  C03C2218/111 ,  C40B40/06

    摘要： A planar, rigid substrate made from a porous, inorganic material coated with cationic polymer molecules for attachment of an array of biomolecules, such as DNA, RNA, oligonucleotides, peptides, and proteins. The substrate has a top surface with about at least 200 to about 200,000 times greater surface area than that of a comparable, non-porous substrate. The cationic polymer molecules are anchored on the top surface and in the pores of the porous material. In high-density applications, an array of polynucleotides of a known, predetermined sequence is attached to this cationic polymer layer, such that each of the polynucleotide is attached to a different localized area on the top surface. The top surface has a surface area for attaching biomolecules of approximately 387,500 cm2/cm2 of area (˜7.5 million cm2/1×3 inch piece of substrate). Each pore of the plurality of pores in the top surface of the substrate has a pore radius of between about 40 Å to about 75 Å. Not only does the cationic coating in and over the pores of the substrate greatly increase the overall positive charge on the substrate surface, but also given the size of the pores provides binding sites to which biomolecules can better attach.

    摘要翻译： 由用于附着生物分子阵列如DNA，RNA，寡核苷酸，肽和蛋白质的阳离子聚合物分子的多孔无机材料制成的平面刚性基底。 衬底具有比可比较的非多孔衬底的表面面积大至少约200至约200,000倍的顶表面。 阳离子聚合物分子锚定在多孔材料的顶表面和孔中。 在高密度应用中，将已知的预定序列的多核苷酸阵列连接到该阳离子聚合物层上，使得每个多核苷酸连接到顶表面上的不同局部区域。 顶表面具有用于附着大约387,500cm 2 / cm 2的面积（约750万厘米2/1/3英寸）的生物分子的表面积 基片）。 衬底的顶表面中的多个孔的每个孔的孔半径在大约40埃至大约75埃之间。 衬底中的阳离子涂层不仅大大增加了衬底表面上的总体正电荷，而且还给出了孔的尺寸提供生物分子可以更好地附着的结合位点。

    Method for fabricating supported bilayer-lipid membranes
    4.
    发明授权
    Method for fabricating supported bilayer-lipid membranes 失效
    制备负载双层脂质膜的方法

    公开(公告)号：US06756078B2

    公开(公告)日：2004-06-29

    申请号：US10228862

    申请日：2002-08-27

    申请人： Dana C. Bookbinder ,  Joydeep Lahiri

    发明人： Dana C. Bookbinder ,  Joydeep Lahiri

    IPC分类号： B05D136

    CPC分类号： B82Y5/00 ,  B01D69/122 ,  B01D71/74 ,  B05D1/185 ,  B82Y30/00 ,  B82Y40/00

    摘要： The present invention relates to a method and composition for producing a supported bilayer-lipid membrane. In one embodiment, the present invention relates to a method and composition for producing a supported membrane. In one embodiment, the present invention relates to a method for producing a supported membrane comprising the steps: (i) providing a substrate coated with a monolayer having reactive functional groups; (ii) contacting the reactive groups with a linker compound to form a derivatized monolayer having covalently bonded linker moieties; and (iii) contacting the derivatized monolayer with a membrane to produce a supported membrane.

    摘要翻译： 本发明涉及一种用于制备负载双层脂质膜的方法和组合物。 在一个实施方案中，本发明涉及用于制备负载膜的方法和组合物。 在一个实施方案中，本发明涉及一种制备负载膜的方法，包括以下步骤：（i）提供涂覆有具有反应性官能团的单层的基材; （ii）使反应性基团与接头化合物接触以形成具有共价连接的接头部分的衍生化单层; 和（iii）使衍生的单层与膜接触以产生负载的膜。

    Method for fabricating supported bilayer-lipid membranes
    5.
    发明授权
    Method for fabricating supported bilayer-lipid membranes 失效
    制备负载双层脂质膜的方法

    公开(公告)号：US07045171B2

    公开(公告)日：2006-05-16

    申请号：US10840713

    申请日：2004-05-05

    申请人： Dana C. Bookbinder ,  Joydeep Lahiri

    发明人： Dana C. Bookbinder ,  Joydeep Lahiri

    IPC分类号： B05D1/36

    CPC分类号： B82Y5/00 ,  B01D69/122 ,  B01D71/74 ,  B05D1/185 ,  B82Y30/00 ,  B82Y40/00

    摘要： The present invention relates to a method and composition for producing a supported membrane. In one embodiment, the present invention relates to a method for producing a supported membrane comprising the steps: (i) providing a substrate coated with a monolayer having reactive functional groups; (ii) contacting the reactive groups with a linker compound to form a derivatized monolayer having covalently bonded linker moieties; and (iii) contacting the derivatized monolayer with a membrane solution to produce a supported membrane.In a further embodiment, the present invention relates to a supported membrane produced by the methods of the present invention.

    摘要翻译： 本发明涉及一种制备负载膜的方法和组合物。 在一个实施方案中，本发明涉及一种制备负载膜的方法，包括以下步骤：（i）提供涂覆有具有反应性官能团的单层的基材; （ii）使反应性基团与连接体化合物接触以形成具有共价键合的接头部分的衍生单层; 和（iii）使衍生的单层与膜溶液接触以产生负载的膜。 在另一个实施方案中，本发明涉及通过本发明的方法制备的支撑膜。

    SUBSTRATES WITH STABLE SURFACE CHEMISTRY FOR BIOLOGICAL MEMBRANE ARRAYS AND METHOD FOR FABRICATING THEREOF
    9.
    发明申请
    SUBSTRATES WITH STABLE SURFACE CHEMISTRY FOR BIOLOGICAL MEMBRANE ARRAYS AND METHOD FOR FABRICATING THEREOF 审中-公开
    用于生物膜阵列的稳定表面化学物质的基底及其制备方法

    公开(公告)号：US20090215650A1

    公开(公告)日：2009-08-27

    申请号：US12437893

    申请日：2009-05-08

    申请人： Ye Fang ,  Anthony G. Frutos ,  Joydeep Lahiri

    发明人： Ye Fang ,  Anthony G. Frutos ,  Joydeep Lahiri

    IPC分类号： C40B40/10

    CPC分类号： G01N33/54393 ,  B01J19/0046 ,  B01J2219/00385 ,  B01J2219/00527 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00635 ,  B01J2219/00659 ,  B01J2219/0074 ,  C40B60/14 ,  G01N33/54366

    摘要： The present invention provides a method for preparing a physically stable array of biological membranes, including membrane proteins, on a surface, and the resultant article of manufacture. The method comprises providing a substrate; creating either a polar surface or reactive surface by coating the substrate with a material that either: (1) enhances the stability of lipid spots during withdrawing through a water/air interface and washing and drying protocols; or (2) gives rise to minimal non-specific binding of a labeled target to a background surface, and high specific binding to a probe receptor in said membrane array, or (3) both; and depositing an array of biological-membrane microspots on the substrate. The method may further comprise applying a reagent that includes a soluable protein to stabilize the biological membranes on the surface. Also provided is an article having biological-membrane microspots that are associated in a stable fashion with a substrate surface embodying these properties.

    摘要翻译： 本发明提供了一种在表面上制备物理上稳定的生物膜阵列，包括膜蛋白的方法，以及所得制品。 该方法包括提供基底; 通过用以下材料涂覆基材来产生极性表面或反应性表面：（1）在通过水/空气界面排出并洗涤和干燥方案期间提高脂质斑点的稳定性; 或（2）导致标记的靶与背景表面的最小非特异性结合，以及所述膜阵列中与探针受体的高特异性结合，或（3）两者; 以及在衬底上沉积生物膜微点阵列。 该方法还可以包括施加包含可溶蛋白质的试剂以稳定表面上的生物膜。 还提供了具有生物膜微点的物品，其以稳定的方式与体现这些性质的基底表面相关联。