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    SUBSTRATES WITH STABLE SURFACE CHEMISTRY FOR BIOLOGICAL MEMBRANE ARRAYS AND METHOD FOR FABRICATING THEREOF
    3.
    发明申请
    SUBSTRATES WITH STABLE SURFACE CHEMISTRY FOR BIOLOGICAL MEMBRANE ARRAYS AND METHOD FOR FABRICATING THEREOF 审中-公开
    用于生物膜阵列的稳定表面化学物质的基底及其制备方法

    公开(公告)号：US20090215650A1

    公开(公告)日：2009-08-27

    申请号：US12437893

    申请日：2009-05-08

    申请人： Ye Fang ,  Anthony G. Frutos ,  Joydeep Lahiri

    发明人： Ye Fang ,  Anthony G. Frutos ,  Joydeep Lahiri

    IPC分类号： C40B40/10

    CPC分类号： G01N33/54393 ,  B01J19/0046 ,  B01J2219/00385 ,  B01J2219/00527 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00635 ,  B01J2219/00659 ,  B01J2219/0074 ,  C40B60/14 ,  G01N33/54366

    摘要： The present invention provides a method for preparing a physically stable array of biological membranes, including membrane proteins, on a surface, and the resultant article of manufacture. The method comprises providing a substrate; creating either a polar surface or reactive surface by coating the substrate with a material that either: (1) enhances the stability of lipid spots during withdrawing through a water/air interface and washing and drying protocols; or (2) gives rise to minimal non-specific binding of a labeled target to a background surface, and high specific binding to a probe receptor in said membrane array, or (3) both; and depositing an array of biological-membrane microspots on the substrate. The method may further comprise applying a reagent that includes a soluable protein to stabilize the biological membranes on the surface. Also provided is an article having biological-membrane microspots that are associated in a stable fashion with a substrate surface embodying these properties.

    摘要翻译： 本发明提供了一种在表面上制备物理上稳定的生物膜阵列，包括膜蛋白的方法，以及所得制品。 该方法包括提供基底; 通过用以下材料涂覆基材来产生极性表面或反应性表面：（1）在通过水/空气界面排出并洗涤和干燥方案期间提高脂质斑点的稳定性; 或（2）导致标记的靶与背景表面的最小非特异性结合，以及所述膜阵列中与探针受体的高特异性结合，或（3）两者; 以及在衬底上沉积生物膜微点阵列。 该方法还可以包括施加包含可溶蛋白质的试剂以稳定表面上的生物膜。 还提供了具有生物膜微点的物品，其以稳定的方式与体现这些性质的基底表面相关联。

    METHODS FOR CHARACTERIZING MOLECULES
    7.
    发明申请
    METHODS FOR CHARACTERIZING MOLECULES 审中-公开
    表征分子的方法

    公开(公告)号：US20100130725A1

    公开(公告)日：2010-05-27

    申请号：US12623693

    申请日：2009-11-23

    申请人： Ye Fang ,  Ann M. Ferrie ,  Joydeep Lahiri ,  Elizabeth Tran

    发明人： Ye Fang ,  Ann M. Ferrie ,  Joydeep Lahiri ,  Elizabeth Tran

    IPC分类号： C07K14/00 ,  C40B30/04 ,  C40B30/06 ,  C40B30/02

    CPC分类号： G01N33/5023 ,  G01N33/5041 ,  G01N33/54373 ,  G01N2800/60 ,  G16B5/00

    摘要： Drug discovery is a complex undertaking facing many challenges, not the least of which is a high attrition rate as many promising candidates prove ineffective or toxic in the clinic owing to a poor understanding of the diseases, and thus the biological systems, they target. Therefore, it is broadly agreed that to increase the productivity of drug discovery one needs a far deeper understanding of the molecular mechanisms of diseases, taking into account the full biological context of the drug target and moving beyond individual genes and proteins. The present methods rely on the use of label-free cellular assays, particularly the DMR index, to systematically display the mode of actions, the toxicity, and the target(s) and pathway(s) of any molecules.

    摘要翻译： 毒品发现是一项面临许多挑战的复杂工作，其中最重要的是高消耗率，因为许多有前途的候选人在诊所中证明无效或有毒，原因是对这些疾病的认识不足，从而导致其生物系统的目标。 因此，普遍同意，为了提高药物发现的生产力，需要更深入地了解疾病的分子机制，同时考虑到药物靶标的完整生物学背景，超越个别基因和蛋白质。 本方法依赖于使用无标记细胞测定法，特别是DMR指数来系统显示任何分子的作用模式，毒性以及靶标和途径。

    Normalization methods for G-protein coupled receptor membrane array
    8.
    发明申请
    Normalization methods for G-protein coupled receptor membrane array 审中-公开
    G蛋白偶联受体膜阵列的归一化方法

    公开(公告)号：US20090131263A1

    公开(公告)日：2009-05-21

    申请号：US11985990

    申请日：2007-11-19

    申请人： Longying Dong ,  Yulong Hong ,  Joydeep Lahiri ,  Fang Lai ,  Li Liu ,  Jeffrey G. Lynn

    发明人： Longying Dong ,  Yulong Hong ,  Joydeep Lahiri ,  Fang Lai ,  Li Liu ,  Jeffrey G. Lynn

    IPC分类号： C40B20/04 ,  C40B40/10 ,  C40B50/14

    CPC分类号： G01N33/554 ,  G01N2333/726 ,  G01N2500/10

    摘要： Reference membrane components are either pre-labeled or labeled during assays for purposes of normalizing signals associated with binding or functional assays employing G-protein coupled receptor microarrays. A reference component may be included in a membrane in which the target GPCR is embedded or may be present in another membrane printed in conjunction with the target membrane on a microspot. Or, a GPCR microarray may be pre-labeled by incorporating a label on an exposed substrate in a defect in the printed microspot.

    摘要翻译： 参考膜组分在测定期间被预标记或标记，目的是使与G-蛋白偶联受体微阵列的结合或功能测定相关的信号归一化。 参考组分可以包括在其中嵌入目标GPCR的膜中，或者可以存在于与微孔上的靶膜结合印刷的另一膜中。 或者，GPCR微阵列可以通过在印刷的微点的缺陷中的暴露的基底上并入标签来进行预标记。