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    Methods of Labeling Cells, Labeled Cells, and uses Thereof
    2.
    发明申请
    Methods of Labeling Cells, Labeled Cells, and uses Thereof 审中-公开
    标记细胞的方法，标记细胞及其用途

    公开(公告)号：US20120178081A1

    公开(公告)日：2012-07-12

    申请号：US13342912

    申请日：2012-01-03

    申请人： Quan N. Nguyen ,  Robert J. Lipshutz ,  Yunqing Ma

    发明人： Quan N. Nguyen ,  Robert J. Lipshutz ,  Yunqing Ma

    IPC分类号： G01N33/53

    CPC分类号： G01N33/54313

    摘要： Methods of detecting nucleic acids, proteins and cells including methods of detecting two or more nucleic acids, proteins and cells in multiplex bDNA assays, are provided. Assays may be conducted at least in vitro, in vivo, in cellulo, and in situ. Nucleic acids are detected, through cooperative hybridization that results in specific association of a label probe system with target nucleic acids. Embodiments are directed to concurrent detection of one or more nucleic acids and/or one or more proteins. The detected proteins may be intracellular or external markers on the surface of the cell. Detection of protein components is accomplished by use of specific antibodies and a label probe system and/or coated microparticles which bind to the outside surface of specific cells and contain specific probes that can be detected using the same label probe system. Compositions, kits, and systems related to the methods are also described.

    摘要翻译： 提供了检测核酸，蛋白质和细胞的方法，包括在多重bDNA测定中检测两种或更多种核酸，蛋白质和细胞的方法。 测定可至少在体外，体内，纤维素和原位进行。 通过协调杂交检测核酸，其导致标记探针系统与靶核酸的特异性结合。 实施方案涉及同时检测一种或多种核酸和/或一种或多种蛋白质。 检测到的蛋白质可以是细胞表面上的细胞内或外部标记。 通过使用特异性抗体和标记探针系统和/或结合特定细胞的外表面并包含可以使用相同标记探针系统检测的特异性探针的包被的微粒来实现蛋白质组分的检测。 还描述了与方法相关的组合物，试剂盒和系统。

    Methods and Compositions for Nucleic Acid Purification
    3.
    发明申请
    Methods and Compositions for Nucleic Acid Purification 审中-公开
    核酸纯化的方法和组合物

    公开(公告)号：US20110237449A1

    公开(公告)日：2011-09-29

    申请号：US12986545

    申请日：2011-01-07

    申请人： Gary K. McMaster ,  Quan N. Nguyen ,  Franklin R. Witney ,  Yunqing Ma

    发明人： Gary K. McMaster ,  Quan N. Nguyen ,  Franklin R. Witney ,  Yunqing Ma

    IPC分类号： C40B30/04 ,  C40B40/06

    CPC分类号： C12Q1/6837 ,  C12Q2565/514 ,  C12Q2537/163 ,  C12Q2525/161

    摘要： Methods of capturing two or more nucleic acids simultaneously from a single sample are provided. Different nucleic acids are captured through cooperative hybridization events on a substrate, or different subsets of particles, or at different selected positions on a spatially addressable solid support. Methods described include enrichment and purification of nucleic acids prior to downstream steps including sequencing of target nucleic acids. Compositions, kits, and systems related to the methods are also described.

    摘要翻译： 提供了从单个样品同时捕获两种或更多种核酸的方法。 不同的核酸通过底物上的协同杂交事件或不同的颗粒子集，或在空间上可寻址的固体支持物上的不同的选定位置捕获。 所描述的方法包括在下游步骤之前的核酸的富集和纯化，包括靶核酸的测序。 还描述了与方法相关的组合物，试剂盒和系统。

    Detection of Nucleic Acids and Proteins
    5.
    发明申请
    Detection of Nucleic Acids and Proteins 审中-公开
    核酸和蛋白质的检测

    公开(公告)号：US20120004132A1

    公开(公告)日：2012-01-05

    申请号：US13176669

    申请日：2011-07-05

    申请人： Aiguo Zhang ,  Yunqing Ma ,  Quan N. Nguyen ,  Franklin R. Witney ,  Gary K. McMaster ,  Glenn H. McGall

    发明人： Aiguo Zhang ,  Yunqing Ma ,  Quan N. Nguyen ,  Franklin R. Witney ,  Gary K. McMaster ,  Glenn H. McGall

    IPC分类号： C40B30/04 ,  G01N33/566 ,  C12Q1/68

    CPC分类号： G01N33/543 ,  C12Q1/682 ,  G01N2458/10 ,  G01N2458/30 ,  C12Q2525/313

    摘要： Methods of detecting various types of nucleic acids, including methods of detecting two or more nucleic acids in multiplex branched-chain DNA assays, are provided. Detection assays may be conducted at least in vitro, in cellulo, and in situ. Nucleic acids which are optionally captured on a solid support are detected, for example, through cooperative hybridization events that result in specific association of a label probe system with the nucleic acids. Various label probe system embodiments are provided. Embodiments are directed to concurrent detection of one or more nucleic acids and one or more proteins. Embodiments also are directed to determining the methylation state of a target sequence. Other embodiments are directed to detection of one or more proteins using DNA barcodes. Compositions, kits, and systems related to the methods are also described.

    摘要翻译： 提供检测各种类型核酸的方法，包括在多重支链DNA测定中检测两种或多种核酸的方法。 检测试验可以至少在体外，纤维素和原位进行。 可以例如通过导致标记探针系统与核酸的特异性缔合的协同杂交事件来检测任选地捕获在固体支持物上的核酸。 提供了各种标签探针系统实施例。 实施方案涉及同时检测一种或多种核酸和一种或多种蛋白质。 实施方案还涉及确定靶序列的甲基化状态。 其他实施方案涉及使用DNA条形码检测一种或多种蛋白质。 还描述了与方法相关的组合物，试剂盒和系统。

    Signal Multiplexing and Signal Amplification
    6.
    发明申请
    Signal Multiplexing and Signal Amplification 审中-公开
    信号复用和信号放大

    公开(公告)号：US20120003648A1

    公开(公告)日：2012-01-05

    申请号：US13175393

    申请日：2011-07-01

    申请人： Yunqing Ma ,  Gary K. McMaster ,  Aiguo Zhang ,  Quan N. Nguyen

    发明人： Yunqing Ma ,  Gary K. McMaster ,  Aiguo Zhang ,  Quan N. Nguyen

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6804 ,  C12Q1/682 ,  C12Q1/6837 ,  C12Q2565/501 ,  C12Q2525/313 ,  C12Q2525/113

    摘要： Disclosed are methods, compositions and kits for amplifying signals for detecting the presence, quantity and/or sequence of nucleic acids and proteins, as well as methods, compositions and kits for increasing the number of such targets simultaneously detectable in a sample. Detection may be, for instance, in vivo, in cellulo or in situ. Amplification of signal is achieved by way of hybridization of nucleic acid label probe systems and structures. Increase in target multiplex capacity is achieved by way of varying the type of labels utilized in the nucleic acid label probe system.

    摘要翻译： 公开了用于扩增用于检测核酸和蛋白质的存在，数量和/或序列的信号的方法，组合物和试剂盒，以及用于增加样品中同时可检测的这些靶标数量的方法，组合物和试剂盒。 检测可以是例如体内，纤维素或原位。 通过核酸标记探针系统和结构的杂交实现信号的扩增。 通过改变在核酸标记探针系统中使用的标记类型来实现目标多重容量的增加。