公开(公告)号：US20160319342A1

公开(公告)日：2016-11-03

申请号：US15061871

申请日：2016-03-04

Applicant: Osaka University ,  Quantum Biosystems Inc.

Inventor： Tomoji Kawai ,  Masateru Taniguchi ,  Takahito Ohshiro ,  Mark Oldham ,  Eric Nordman

IPC: C12Q1/68 ,  G01N27/04 ,  G06F19/22

CPC classification number: C12Q1/6869 ,  G01N27/04 ,  G01N33/48721 ,  G06F19/22 ,  C12Q2563/116 ,  C12Q2565/631

Abstract: Devices, systems and methods for sequencing protein samples are provided. In some examples, currents generated when a monomer passes through between electrodes of a nanogap electrode pair are measured for each of several different distances, so that monomers are identified when compared to a reference physical quantity of a known monomer, which may be obtained from a current measured with a similar inter-electrode distance(s) at which each of plural kinds of monomers are identifiable and ordered with predetermined accuracy and based on a detected physical quantity obtained from a tunneling current, which may be further normalized by the use of one or more reference substances.

Abstract translation: 提供了用于测序蛋白质样品的设备，系统和方法。 在一些实例中，当在几个不同距离中的每一个测量单体通过纳米缝电极对的电极之间产生的电流，使得当与已知单体的参考物理量相比时，识别单体，其可以从 以类似的电极间距测量的电流，其中可以以预定精度识别和排序多种单体中的每一种，并且基于从隧道电流获得的检测到的物理量，其可以通过使用一个 或更多的参考物质。

公开(公告)号：US20180023132A1

公开(公告)日：2018-01-25

申请号：US15448317

申请日：2017-03-02

Applicant: Quantum Biosystems Inc.

Inventor： Tomoji Kawai ,  Masateru Taniguchi ,  Takahito Ohshiro ,  Mark Oldham ,  Eric Nordman

IPC: C12Q1/68 ,  G06F19/22 ,  G01N27/04

Abstract: Devices, systems and methods for sequencing protein samples are provided. In some examples, currents generated when a monomer passes through between electrodes of a nanogap electrode pair are measured for each of several different distances, so that monomers are identified when compared to a reference physical quantity of a known monomer, which may be obtained from a current measured with a similar inter-electrode distance(s) at which each of plural kinds of monomers are identifiable and ordered with predetermined accuracy and based on a detected physical quantity obtained from a tunneling current, which may be further normalized by the use of one or more reference substances.

公开(公告)号：US09644236B2

公开(公告)日：2017-05-09

申请号：US15061871

申请日：2016-03-04

Applicant: Quantum Biosystems Inc.

Inventor： Tomoji Kawai ,  Masateru Taniguchi ,  Takahito Ohshiro ,  Mark Oldham ,  Eric Nordman

IPC: C12Q1/68 ,  G01N27/64 ,  G06F19/22 ,  G01N27/04

CPC classification number: C12Q1/6869 ,  G01N27/04 ,  G01N33/48721 ,  G06F19/22 ,  C12Q2563/116 ,  C12Q2565/631

Abstract: Devices, systems and methods for sequencing protein samples are provided. In some examples, currents generated when a monomer passes through between electrodes of a nanogap electrode pair are measured for each of several different distances, so that monomers are identified when compared to a reference physical quantity of a known monomer, which may be obtained from a current measured with a similar inter-electrode distance(s) at which each of plural kinds of monomers are identifiable and ordered with predetermined accuracy and based on a detected physical quantity obtained from a tunneling current, which may be further normalized by the use of one or more reference substances.

公开(公告)号：US20160245789A1

公开(公告)日：2016-08-25

申请号：US15048810

申请日：2016-02-19

Applicant: Quantum Biosystems Inc.

Inventor： Shuji Ikeda ,  Mark Oldham ,  Eric Nordman

IPC: G01N33/487 ,  C23C16/34 ,  C12Q1/68 ,  C23C16/56 ,  C23C14/06 ,  C23C14/58 ,  G01N27/447 ,  C23C16/06

CPC classification number: G01N33/48721 ,  B82Y40/00 ,  C12Q1/6869 ,  C23C14/0641 ,  C23C14/5886 ,  C23C16/06 ,  C23C16/345 ,  C23C16/56 ,  G01N27/3278 ,  G01N27/44704 ,  G01N27/44791

Abstract: The present disclosure provides methods for forming a nano-gap electrode. In some cases, a nano-gap having a width adjusted by a film thickness of a sidewall may be formed between a first electrode-forming part and a second electrode-forming part using sidewall which has contact with first electrode-forming part as a mask. Surfaces of the first electrode-forming part, the sidewall and the second electrode-forming part may then be exposed. The sidewall may then be removed to form a nano-gap between the first electrode-forming part and the second electrode-forming part.

Abstract translation: 本公开提供了形成纳米间隙电极的方法。 在一些情况下，可以使用与第一电极形成部分接触的侧壁作为掩模，在第一电极形成部分和第二电极形成部分之间形成具有由侧壁的膜厚度调节的宽度的纳米间隙 。 然后可以露出第一电极形成部分，侧壁和第二电极形成部分的表面。 然后可以去除侧壁以在第一电极形成部分和第二电极形成部分之间形成纳米间隙。

公开(公告)号：US10557167B2

公开(公告)日：2020-02-11

申请号：US15448317

申请日：2017-03-02

Applicant: Quantum Biosystems Inc.

Inventor： Tomoji Kawai ,  Masateru Taniguchi ,  Takahito Ohshiro ,  Mark Oldham ,  Eric Nordman

IPC: C12Q1/68 ,  G06F19/22 ,  G01N27/04 ,  C12Q1/6869 ,  G16B30/00

Abstract: Devices, systems and methods for sequencing protein samples are provided. In some examples, currents generated when a monomer passes through between electrodes of a nanogap electrode pair are measured for each of several different distances, so that monomers are identified when compared to a reference physical quantity of a known monomer, which may be obtained from a current measured with a similar inter-electrode distance(s) at which each of plural kinds of monomers are identifiable and ordered with predetermined accuracy and based on a detected physical quantity obtained from a tunneling current, which may be further normalized by the use of one or more reference substances.

公开(公告)号：US20190242846A1

公开(公告)日：2019-08-08

申请号：US16266363

申请日：2019-02-04

Applicant: Quantum Biosystems Inc. ,  Osaka University

Inventor： Masateru TANIGUCHI ,  Takahito Ohshiro ,  Makusu Tsutsui ,  Kazumichi Yokota ,  Toshiki Matsuoka ,  Mark Oldham ,  Eric Nordman

IPC: G01N27/327 ,  G01N33/487

CPC classification number: G01N27/3278 ,  B82Y40/00 ,  C12M1/00 ,  C12Q1/6869 ,  G01N33/48721

Abstract: Methods and systems are provided for creation of stable and consistent nanoelectrode pairs for detection of biomolecules, such as deoxyribonucleic acid.

公开(公告)号：US20160245790A1

公开(公告)日：2016-08-25

申请号：US15048889

申请日：2016-02-19

Applicant: Osaka University ,  Quantum Biosystems Inc.

Inventor： Tomoji Kawai ,  Masayuki Furuhashi ,  Masateru Kawaguchi ,  Mark Oldham ,  Eric Nordman

IPC: G01N33/487 ,  G01N27/26 ,  C12Q1/68 ,  B01L3/00 ,  B01L7/00

CPC classification number: G01N33/48721 ,  B01L3/502715 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/0663 ,  B01L2200/10 ,  B01L2300/04 ,  B01L2300/0645 ,  B01L2300/0663 ,  B01L2300/0816 ,  B01L2300/0896 ,  B01L2300/12 ,  B01L2300/1827 ,  B01L2400/086 ,  C12Q1/6813 ,  C12Q1/6869 ,  G01N27/26 ,  G01N27/3278 ,  C12Q2527/107 ,  C12Q2527/137 ,  C12Q2563/159 ,  C12Q2565/629 ,  C12Q2565/631

Abstract: The present disclosure provides methods and systems that can reduce the amount of sample necessary to detect or identify, or both detect and identify, a biomolecule, and increase the rate of denaturing of the biomolecule. A device for thermally denaturing a biomolecule may include: a substrate having low thermal conductivity; a heater disposed adjacent to the substrate; a temperature sensor disposed adjacent to the substrate; a semiconductor oxide film disposed adjacent to the substrate, a nanochannel formed in a region of the semiconductor oxide film, and a cover over the nanochannel.

Abstract translation: 本公开提供了可以减少检测或识别或既检测和鉴定生物分子又增加生物分子的变性速率所需的样品量的方法和系统。 用于热变性生物分子的装置可以包括：具有低导热性的基底; 靠近所述基板设置的加热器; 设置在所述基板附近的温度传感器; 邻近衬底设置的半导体氧化膜，形成在半导体氧化膜的区域中的纳米通道以及纳米通道上的覆盖层。