-
公开(公告)号：US20110281738A1
公开(公告)日：2011-11-17
申请号：US13098965
申请日：2011-05-02
CPC分类号： C12Q1/6837 , C12N15/1093 , C12Q1/682 , C12Q1/6869 , C12Q1/6874 , C12Q2531/125 , C12Q2525/313 , C12Q2525/151 , C12Q2521/313 , C12Q2565/513 , C12Q2521/307
摘要： The present invention provides methods of making and using self-assembled arrays of single polynucleotide molecules for carrying out a variety of large-scale genetic measurements, such as gene expression analysis, gene copy number assessment, and the like. Random arrays used in the invention are “self-assembled” in the sense that they are formed by deposition of polynucleotide molecules onto a surface where they become fixed at random locations. The polynucleotide molecules fixed on the surface are then identified by direct sequence determination of component nucleic acids, such as incorporated probe sequences, or by other decoding schemes. Such identification converts a random array of determinable polynucleotides, and their respective probes into an addressable array of probe sequences.
摘要翻译： 本发明提供制造和使用单个多核苷酸分子的自组装阵列进行各种大规模遗传测量的方法，例如基因表达分析，基因拷贝数评估等。 在本发明中使用的随机阵列是“自组装”的意思，即它们通过将多核苷酸分子沉积到其随机位置固定的表面上而形成。 然后通过直接序列测定组分核酸，例如并入的探针序列或其他解码方案来鉴定固定在表面上的多核苷酸分子。 这种鉴定将随机阵列的可测定多核苷酸及其各自的探针转换成可寻址的探针序列阵列。
-
公开(公告)号：US08609335B2
公开(公告)日：2013-12-17
申请号：US13098965
申请日：2011-05-02
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , C12N15/1093 , C12Q1/682 , C12Q1/6869 , C12Q1/6874 , C12Q2531/125 , C12Q2525/313 , C12Q2525/151 , C12Q2521/313 , C12Q2565/513 , C12Q2521/307
摘要： The present invention provides methods of making and using self-assembled arrays of single polynucleotide molecules for carrying out a variety of large-scale genetic measurements, such as gene expression analysis, gene copy number assessment, and the like. Random arrays used in the invention are “self-assembled” in the sense that they are formed by deposition of polynucleotide molecules onto a surface where they become fixed at random locations. The polynucleotide molecules fixed on the surface are then identified by direct sequence determination of component nucleic acids, such as incorporated probe sequences, or by other decoding schemes. Such identification converts a random array of determinable polynucleotides, and their respective probes into an addressable array of probe sequences.
摘要翻译： 本发明提供制造和使用单个多核苷酸分子的自组装阵列进行各种大规模遗传测量的方法，例如基因表达分析，基因拷贝数评估等。 在本发明中使用的随机阵列是“自组装”的意思，即它们通过将多核苷酸分子沉积到其随机位置固定的表面上而形成。 然后通过直接序列测定组分核酸，例如并入的探针序列或其他解码方案来鉴定固定在表面上的多核苷酸分子。 这种鉴定将随机阵列的可测定多核苷酸及其各自的探针转换成可寻址的探针序列阵列。
-
公开(公告)号：US08445194B2
公开(公告)日：2013-05-21
申请号：US11451691
申请日：2006-06-13
CPC分类号： C12Q1/6874 , C07H21/04 , C07K1/047 , C12Q1/6806 , C12Q1/682 , C12Q1/6837 , C12Q1/6869 , C12Q2525/151 , C12Q2525/313 , C12Q2531/125 , C12Q2565/513 , G01N15/1404 , G01N15/1434 , Y10S977/778 , Y10S977/789 , Y10S977/792 , Y10S977/88 , Y10S977/882 , C12Q2521/307 , C12Q2525/161 , C12Q2535/122 , C12Q2563/179 , C12Q2565/514
摘要： Random arrays of single molecules are provided for carrying out large scale analyses, particularly of biomolecules, such as genomic DNA, cDNAs, proteins, and the like. In one aspect, arrays of the invention comprise concatemers of DNA fragments that are randomly disposed on a regular array of discrete spaced apart regions, such that substantially all such regions contain no more than a single concatemer. Preferably, such regions have areas substantially less than 1 μm2 and have nearest neighbor distances that permit optical resolution of on the order of 109 single molecules per cm2. Many analytical chemistries can be applied to random arrays of the invention, including sequencing by hybridization chemistries, sequencing by synthesis chemistries, SNP detection chemistries, and the like, to greatly expand the scale and potential applications of such techniques.
-
公开(公告)号：US08133719B2
公开(公告)日：2012-03-13
申请号：US11981607
申请日：2007-10-31
CPC分类号： C12Q1/6874 , C07H21/04 , C07K1/047 , C12Q1/6806 , C12Q1/682 , C12Q1/6837 , C12Q1/6869 , C12Q2525/151 , C12Q2525/313 , C12Q2531/125 , C12Q2565/513 , G01N15/1404 , G01N15/1434 , Y10S977/778 , Y10S977/789 , Y10S977/792 , Y10S977/88 , Y10S977/882 , C12Q2521/307 , C12Q2525/161 , C12Q2535/122 , C12Q2563/179 , C12Q2565/514
摘要： Random arrays of single molecules are provided for carrying out large scale analyses, particularly of biomolecules, such as genomic DNA, cDNAs, proteins, and the like. In one aspect, arrays of the invention comprise concatemers of DNA fragments that are randomly disposed on a regular array of discrete spaced apart regions, such that substantially all such regions contain no more than a single concatemer. Preferably, such regions have areas substantially less than 1 μm2 and have nearest neighbor distances that permit optical resolution of on the order of 109 single molecules per cm2. Many analytical chemistries can be applied to random arrays of the invention, including sequencing by hybridization chemistries, sequencing by synthesis chemistries, SNP detection chemistries, and the like, to greatly expand the scale and potential applications of such techniques.
摘要翻译： 提供单个分子的随机阵列用于进行大规模分析，特别是生物分子，例如基因组DNA，cDNAs，蛋白质等。 在一个方面，本发明的阵列包括随机设置在离散间隔开的区域的规则阵列上的DNA片段的并列物质，使得基本上所有这些区域都包含不超过单个并联体。 优选地，这样的区域具有基本上小于1μm2的面积，并且具有最近的相邻距离，其允许每厘米每分钟109个单分子的光学分辨率。 许多分析化学物质可以应用于本发明的随机阵列，包括杂交化学测序，合成化学测序，SNP检测化学等，以大大扩展这些技术的规模和潜在应用。
-
公开(公告)号：US20100081128A1
公开(公告)日：2010-04-01
申请号：US11541225
申请日：2006-09-29
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , C12N15/1093 , C12Q1/682 , C12Q1/6869 , C12Q1/6874 , C12Q2531/125 , C12Q2525/313 , C12Q2525/151 , C12Q2521/313 , C12Q2565/513 , C12Q2521/307
摘要： The present invention provides methods of making and using self-assembled arrays of single polynucleotide molecules for carrying out a variety of large-scale genetic measurements, such as gene expression analysis, gene copy number assessment, and the like. Random arrays used in the invention are “self-assembled” in the sense that they are formed by deposition of polynucleotide molecules onto a surface where they become fixed at random locations. The polynucleotide molecules fixed on the surface are then identified by direct sequence determination of component nucleic acids, such as incorporated probe sequences, or by other decoding schemes. Such identification converts a random array of determinable polynucleotides, and their respective probes into an addressable array of probe sequences.
摘要翻译： 本发明提供制造和使用单个多核苷酸分子的自组装阵列进行各种大规模遗传测量的方法，例如基因表达分析，基因拷贝数评估等。 在本发明中使用的随机阵列是“自组装”的意思，即它们通过将多核苷酸分子沉积到其随机位置固定的表面上而形成。 然后通过直接序列测定组分核酸，例如并入的探针序列或其他解码方案来鉴定固定在表面上的多核苷酸分子。 这种鉴定将随机阵列的可测定多核苷酸及其各自的探针转换成可寻址的探针序列阵列。
-
公开(公告)号：US08445197B2
公开(公告)日：2013-05-21
申请号：US11982467
申请日：2007-10-31
CPC分类号： C12Q1/6874 , C07H21/04 , C07K1/047 , C12Q1/6806 , C12Q1/682 , C12Q1/6837 , C12Q1/6869 , C12Q2525/151 , C12Q2525/313 , C12Q2531/125 , C12Q2565/513 , G01N15/1404 , G01N15/1434 , Y10S977/778 , Y10S977/789 , Y10S977/792 , Y10S977/88 , Y10S977/882 , C12Q2521/307 , C12Q2525/161 , C12Q2535/122 , C12Q2563/179 , C12Q2565/514
摘要： Random arrays of single molecules are provided for carrying out large scale analyses, particularly of biomolecules, such as genomic DNA, cDNAs, proteins, and the like. In one aspect, arrays of the invention comprise concatemers of DNA fragments that are randomly disposed on a regular array of discrete spaced apart regions, such that substantially all such regions contain no more than a single concatemer. Preferably, such regions have areas substantially less than 1 μm2 and have nearest neighbor distances that permit optical resolution of on the order of 109 single molecules per cm2. Many analytical chemistries can be applied to random arrays of the invention, including sequencing by hybridization chemistries, sequencing by synthesis chemistries, SNP detection chemistries, and the like, to greatly expand the scale and potential applications of such techniques.
-
公开(公告)号：US20080234136A1
公开(公告)日：2008-09-25
申请号：US11981607
申请日：2007-10-31
CPC分类号： C12Q1/6874 , C07H21/04 , C07K1/047 , C12Q1/6806 , C12Q1/682 , C12Q1/6837 , C12Q1/6869 , C12Q2525/151 , C12Q2525/313 , C12Q2531/125 , C12Q2565/513 , G01N15/1404 , G01N15/1434 , Y10S977/778 , Y10S977/789 , Y10S977/792 , Y10S977/88 , Y10S977/882 , C12Q2521/307 , C12Q2525/161 , C12Q2535/122 , C12Q2563/179 , C12Q2565/514
摘要： Random arrays of single molecules are provided for carrying out large scale analyses, particularly of biomolecules, such as genomic DNA, cDNAs, proteins, and the like. In one aspect, arrays of the invention comprise concatemers of DNA fragments that are randomly disposed on a regular array of discrete spaced apart regions, such that substantially all such regions contain no more than a single concatemer. Preferably, such regions have areas substantially less than 1 μm2 and have nearest neighbor distances that permit optical resolution of on the order of 109 single molecules per cm2. Many analytical chemistries can be applied to random arrays of the invention, including sequencing by hybridization chemistries, sequencing by synthesis chemistries, SNP detection chemistries, and the like, to greatly expand the scale and potential applications of such techniques.
摘要翻译： 提供单个分子的随机阵列用于进行大规模分析，特别是生物分子，例如基因组DNA，cDNAs，蛋白质等。 在一个方面，本发明的阵列包括随机设置在离散间隔开的区域的规则阵列上的DNA片段的并列物质，使得基本上所有这些区域都包含不超过单个并联体。 优选地，这样的区域具有基本上小于1mum 2的面积，并且具有最接近的距离，其允许每厘米2的单分子的光学分辨率 。 许多分析化学物质可以应用于本发明的随机阵列，包括杂交化学测序，合成化学测序，SNP检测化学等，以大大扩展这些技术的规模和潜在应用。
-
公开(公告)号：US07960104B2
公开(公告)日：2011-06-14
申请号：US11541225
申请日：2006-09-29
CPC分类号： C12Q1/6837 , C12N15/1093 , C12Q1/682 , C12Q1/6869 , C12Q1/6874 , C12Q2531/125 , C12Q2525/313 , C12Q2525/151 , C12Q2521/313 , C12Q2565/513 , C12Q2521/307
摘要： The present invention provides methods of making and using self-assembled arrays of single polynucleotide molecules for carrying out a variety of large-scale genetic measurements, such as gene expression analysis, gene copy number assessment, and the like. Random arrays used in the invention are “self-assembled” in the sense that they are formed by deposition of polynucleotide molecules onto a surface where they become fixed at random locations. The polynucleotide molecules fixed on the surface are then identified by direct sequence determination of component nucleic acids, such as incorporated probe sequences, or by other decoding schemes. Such identification converts a random array of determinable polynucleotides, and their respective probes into an addressable array of probe sequences.
摘要翻译： 本发明提供制造和使用单个多核苷酸分子的自组装阵列进行各种大规模遗传测量的方法，例如基因表达分析，基因拷贝数评估等。 在本发明中使用的随机阵列是“自组装”的意思，即它们通过将多核苷酸分子沉积到其随机位置固定的表面上而形成。 然后通过直接序列测定组分核酸，例如并入的探针序列或其他解码方案来鉴定固定在表面上的多核苷酸分子。 这种鉴定将随机阵列的可测定多核苷酸及其各自的探针转换成可寻址的探针序列阵列。
-
公开(公告)号：US08440397B2
公开(公告)日：2013-05-14
申请号：US11981761
申请日：2007-10-31
CPC分类号： C12Q1/6874 , C12N15/64 , C12N15/66 , Y10T436/143333 , C12Q2521/313 , C12Q2525/191 , C12Q2525/151 , C12Q2525/131 , C12Q2565/518 , C12Q2533/107 , C12Q2531/125
摘要： The present invention is directed to methods and compositions for acquiring nucleotide sequence information of target sequences using adaptors interspersed in target polynucleotides. The sequence information can be new, e.g. sequencing unknown nucleic acids, re-sequencing, or genotyping. The invention preferably includes methods for inserting a plurality of adaptors at spaced locations within a target polynucleotide or a fragment of a polynucleotide. Such adaptors may serve as platforms for interrogating adjacent sequences using various sequencing chemistries, such as those that identify nucleotides by primer extension, probe ligation, and the like. Encompassed in the invention are methods and compositions for the insertion of known adaptor sequences into target sequences, such that there is an interruption of contiguous target sequence with the adaptors. By sequencing both “upstream” and “downstream” of the adaptors, identification of entire target sequences may be accomplished.
-
公开(公告)号：US20120100534A1
公开(公告)日：2012-04-26
申请号：US12329365
申请日：2008-12-05
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6855 , C12Q2531/125 , C12Q2521/313 , C12Q2521/125 , C12Q2537/163 , C12Q2525/191 , C12Q2521/531 , C12Q2521/501 , C12Q2525/307 , C12Q2525/151
摘要： The present invention is directed to compositions and methods for nucleic acid identification and detection. Compositions and methods of the present invention include extracting and fragmenting target nucleic acids from a sample, using the fragmented target nucleic acids to produce target nucleic acid templates and subjecting those target nucleic acid templates to amplification methods to form nucleic acid nanoballs. The invention also includes methods of detecting and identifying sequences using various sequencing applications, including sequencing by ligation methods.
摘要翻译： 本发明涉及用于核酸鉴定和检测的组合物和方法。 本发明的组合物和方法包括从样品中提取和分离靶核酸，使用片段化的靶核酸产生靶核酸模板，并使这些靶核酸模板进行扩增方法以形成核酸纳米棒。 本发明还包括使用各种测序应用检测和鉴定序列的方法，包括通过连接方法测序。
-
-
-
-
-
-
-
-
-
搜索结果
89 条记录 (耗时 0.041 秒)
