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15 条记录 (耗时 0.022 秒)

    Noninvasive imaging of nucleic acid vectors
    5.
    发明授权
    Noninvasive imaging of nucleic acid vectors 失效
    核酸载体的无创成像

    公开(公告)号：US06284220B1

    公开(公告)日：2001-09-04

    申请号：US09324665

    申请日：1999-06-03

    申请人： Alexei Bogdanov ,  Ching-Hsuan Tung ,  Ralph Weissleder

    发明人： Alexei Bogdanov ,  Ching-Hsuan Tung ,  Ralph Weissleder

    IPC分类号： A61K5100

    CPC分类号： C07D493/04 ,  A61K49/0021 ,  A61K49/0047 ,  A61K49/0056

    摘要： Nucleic acid-imaging compositions and methods for noninvasive imaging of a nucleic acid introduced into somatic tissues of an animal or human are disclosed. The noninvasive imaging enables quantitative assessment of the biodistribution of the introduced nucleic acid. The disclosed imaging compounds include a base-binding moiety, a phosphate-binding moiety, and a metal-binding moiety. A chelated metal is non-invasively detected for imaging by radioactivity or magnetic resonance.

    摘要翻译： 公开了核酸成像组合物和引入动物或人的体细胞组织的核酸无创成像的方法。 无创成像能够定量评估引入的核酸的生物分布。 公开的成像化合物包括碱结合部分，磷酸结合部分和金属结合部分。 通过放射性或磁共振来非侵入性地检测螯合金属进行成像。

    Graft co-polymer adducts of platinum (II) compounds
    8.
    发明授权
    Graft co-polymer adducts of platinum (II) compounds 失效
    铂（II）化合物的接枝共聚物加合物

    公开(公告)号：US5871710A

    公开(公告)日：1999-02-16

    申请号：US738177

    申请日：1996-10-28

    申请人： Alexei Bogdanov ,  Ralph Weissleder ,  Thomas J. Brady

    发明人： Alexei Bogdanov ,  Ralph Weissleder ,  Thomas J. Brady

    IPC分类号： A61K47/48 ,  A61K51/08 ,  A61K31/74 ,  A61K49/00 ,  A61K37/14

    CPC分类号： A61K47/48169 ,  Y10S977/795 ,  Y10S977/904 ,  Y10S977/926 ,  Y10S977/927 ,  Y10S977/93

    摘要： A biocompatible graft co-polymer adduct including a polymeric carrier, a protective chain linked to the polymeric carrier, a reporter group linked to the carrier or to the carrier and the protective chain, and a reversibly linked Pt(II) compound. The invention also relates to a method of treating a disease in a patient, particularly cancer, by administering to the patient a therapeutically effective amount of the adduct, and may include scanning the patient using an imaging technique which can provide a visible image of the distribution of the adduct.

    摘要翻译： 生物相容的接枝共聚物加合物，包括聚合物载体，与聚合物载体连接的保护链，与载体或载体和保护链连接的报道基团，以及可逆连接的Pt（II）化合物。 本发明还涉及通过向患者施用治疗有效量的加合物来治疗患者，特别是癌症中的疾病的方法，并且可以包括使用能够提供分布的可见图像的成像技术扫描患者 的加合物。

    Hyperbranched dendron and methods of synthesis and use thereof
    9.
    发明授权
    Hyperbranched dendron and methods of synthesis and use thereof 失效
    超分支树突及其合成和使用方法

    公开(公告)号：US07153905B2

    公开(公告)日：2006-12-26

    申请号：US10806025

    申请日：2004-03-22

    申请人： Pallab Banerjee ,  Wilfried Reichardt ,  Ralph Weissleder ,  Alexei Bogdanov

    发明人： Pallab Banerjee ,  Wilfried Reichardt ,  Ralph Weissleder ,  Alexei Bogdanov

    IPC分类号： C08F8/30

    CPC分类号： C08F283/00 ,  C08F271/00 ,  C08F283/04 ,  C08G73/0206 ,  C08G83/003 ,  C12N15/87 ,  Y10T428/31848

    摘要： Hyperbranched dendron (HD) polymers are synthesized using low molecular weight polyethyleneimine (BPEI-L) as a core and used for gene delivery. The obtained polymers display low toxicity and efficient gene delivery at low nitrogen-to-phosphate (N/P) ratios. Using successive attachment of ethyleneimine moieties to a PEI core, the polymer has a lower relative ratio of linear-to-branched structures than in the core PEI. The more extensive branching enables the polymer to condense plasmid DNA into nanostructure complexes with a size of less than or equal to about 100 nm. The complexes are stable and efficient in transfecting cells in the presence of serum. Bioluminescent imaging of in vivo gene expression using a luciferase reporter gene performed in live mice showed gene expression in the liver and in submandibular lymph nodes.

    摘要翻译： 使用低分子量聚乙烯亚胺（BPEI-L）作为核心并用于基因递送合成超支化树突（HD）聚合物。 所获得的聚合物在低氮磷酸盐（N / P）比低的情况下显示低毒性和有效的基因递送。 使用乙烯亚胺部分连续连接到PEI芯上，该聚合物具有比在芯PEI中更低的线性至支化结构的相对比例。 更广泛的分支使得聚合物能够将质粒DNA冷凝成尺寸小于或等于约100nm的纳米结构复合物。 复合物在血清存在下转染细胞是稳定和有效的。 使用在活鼠中进行的荧光素酶报告基因的体内基因表达的生物发光成像显示在肝脏和下颌下淋巴结中的基因表达。

    Imaging of enzymatic activity
    10.
    发明授权
    Imaging of enzymatic activity 失效

    公开(公告)号：US06737247B2

    公开(公告)日：2004-05-18

    申请号：US09999665

    申请日：2001-10-19

    申请人： Alexei Bogdanov ,  Ralph Weissleder

    发明人： Alexei Bogdanov ,  Ralph Weissleder

    IPC分类号： C12Q126

    CPC分类号： G01R33/48 ,  G01R33/465

    摘要： The invention features methods of detecting enzymatic activity (e.g., in a magnetic resonance image). In general, the methods include: (1) providing a monomeric substrate (e.g., a substrate that is polymerizable in the presence of an enzyme or as a result of an enzyme-catalyzed reaction), having the generic structure X-Y-Z, where X includes a chelator moiety having a chelated paramagnetic or superparamagnetic metal atom or ion, Y includes a linker moiety (e.g., to provide a covalent or non-covalent chemical bond or bonds between X and Z), and Z includes a polymerizing moiety; (2) contacting the substrate with a target tissue, wherein the substrate undergoes polymerization to form a paramagnetic or superparamagnetic polymer, the polymerization being catalyzed by an enzyme in an extracellular matrix or bound to the surfaces of cells of the target tissue; and (3) detecting an increase in relaxivity for the polymer relative to an equivalent amount of unpolymerized substrate. The invention also features substrate compositions.