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公开(公告)号:US5871710A
公开(公告)日:1999-02-16
申请号:US738177
申请日:1996-10-28
CPC分类号: A61K47/48169 , Y10S977/795 , Y10S977/904 , Y10S977/926 , Y10S977/927 , Y10S977/93
摘要: A biocompatible graft co-polymer adduct including a polymeric carrier, a protective chain linked to the polymeric carrier, a reporter group linked to the carrier or to the carrier and the protective chain, and a reversibly linked Pt(II) compound. The invention also relates to a method of treating a disease in a patient, particularly cancer, by administering to the patient a therapeutically effective amount of the adduct, and may include scanning the patient using an imaging technique which can provide a visible image of the distribution of the adduct.
摘要翻译: 生物相容的接枝共聚物加合物,包括聚合物载体,与聚合物载体连接的保护链,与载体或载体和保护链连接的报道基团,以及可逆连接的Pt(II)化合物。 本发明还涉及通过向患者施用治疗有效量的加合物来治疗患者,特别是癌症中的疾病的方法,并且可以包括使用能够提供分布的可见图像的成像技术扫描患者 的加合物。
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公开(公告)号:US20080044827A1
公开(公告)日:2008-02-21
申请号:US11631720
申请日:2005-07-07
申请人: Alexei Bogdanov , John Chen , Ralph Weissleder , Manel Querol
发明人: Alexei Bogdanov , John Chen , Ralph Weissleder , Manel Querol
IPC分类号: C12Q1/68 , C07C39/04 , C07D209/04 , C07D257/00 , G01N33/53 , C12Q1/00 , C12Q1/26 , C12Q1/28
CPC分类号: G01N33/5088 , G01N33/5014 , G01N33/5067
摘要: This invention relates to biochemistry and magnetic resonance imaging.
摘要翻译: 本发明涉及生物化学和磁共振成像。
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公开(公告)号:US08486373B2
公开(公告)日:2013-07-16
申请号:US10979755
申请日:2004-11-01
CPC分类号: A61K49/0056 , A61B5/0068 , A61B5/0071 , A61B5/0084 , A61B5/0086 , A61K47/60 , A61K49/0032 , A61K49/0054 , A61K49/0058 , C12Q1/6841 , C12Q1/6883 , C12Q2565/30 , C12Q2565/1015 , C12Q2525/301
摘要: An intramolecularly-quenched, near infrared fluorescence probe that emits substantial fluorescence only after interaction with a target tissue (i.e., activation) is disclosed. The probe includes a polymeric backbone and a plurality of near infrared fluorochromes covalently linked to the backbone at fluorescence-quenching interaction-permissive positions separable by enzymatic cleavage at fluorescence activation sites. The probe optionally includes protective chains or fluorochrome spacers, or both. Also disclosed are methods of using the intramolecularly-quenched, near infrared fluorescence probes for in vivo optical imaging.
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公开(公告)号:US20060258804A1
公开(公告)日:2006-11-16
申请号:US11431996
申请日:2006-05-11
IPC分类号: C08L53/00
CPC分类号: C08F283/00 , C08F271/00 , C08F283/04 , C08G73/0206 , C08G83/003 , C12N15/87 , Y10T428/31848
摘要: Hyperbranched dendron (HD) polymers are synthesized using low molecular weight polyethyleneimine (BPEI-L) as a core and used for gene delivery. The obtained polymers display low toxicity and efficient gene delivery at low nitrogen-to-phosphate (N/P) ratios. Using successive attachment of ethyleneimine moieties to a PEI core, the polymer has a lower relative ratio of linear-to-branched structures than in the core PEI. The more extensive branching enables the polymer to condense plasmid DNA into nanostructure complexes with a size of less than or equal to about 100 nm. The complexes are stable and efficient in transfecting cells in the presence of serum. Bioluminescent imaging of in vivo gene expression using a luciferase reporter gene performed in live mice showed gene expression in the liver and in submandibular lymph nodes.
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公开(公告)号:US06284220B1
公开(公告)日:2001-09-04
申请号:US09324665
申请日:1999-06-03
IPC分类号: A61K5100
CPC分类号: C07D493/04 , A61K49/0021 , A61K49/0047 , A61K49/0056
摘要: Nucleic acid-imaging compositions and methods for noninvasive imaging of a nucleic acid introduced into somatic tissues of an animal or human are disclosed. The noninvasive imaging enables quantitative assessment of the biodistribution of the introduced nucleic acid. The disclosed imaging compounds include a base-binding moiety, a phosphate-binding moiety, and a metal-binding moiety. A chelated metal is non-invasively detected for imaging by radioactivity or magnetic resonance.
摘要翻译: 公开了核酸成像组合物和引入动物或人的体细胞组织的核酸无创成像的方法。 无创成像能够定量评估引入的核酸的生物分布。 公开的成像化合物包括碱结合部分,磷酸结合部分和金属结合部分。 通过放射性或磁共振来非侵入性地检测螯合金属进行成像。
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公开(公告)号:US6083486A
公开(公告)日:2000-07-04
申请号:US79447
申请日:1998-05-14
CPC分类号: A61K49/0054 , A61K49/0032 , A61K49/0056 , A61K49/0058
摘要: An intramolecularly-quenched, near infrared fluorescence probe that emits substantial fluorescence only after interaction with a target tissue (i.e., activation) is disclosed. The probe includes a polymeric backbone and a plurality of near infrared fluorochromes covalently linked to the backbone at fluorescence-quenching interaction-permissive positions separable by enzymatic cleavage at fluorescence activation sites. The probe optionally includes protective chains or fluorochrome spacers, or both. Also disclosed are methods of using the intramolecularly-quenched, near infrared fluorescence probes for in vivo optical imaging.
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公开(公告)号:US07153905B2
公开(公告)日:2006-12-26
申请号:US10806025
申请日:2004-03-22
IPC分类号: C08F8/30
CPC分类号: C08F283/00 , C08F271/00 , C08F283/04 , C08G73/0206 , C08G83/003 , C12N15/87 , Y10T428/31848
摘要: Hyperbranched dendron (HD) polymers are synthesized using low molecular weight polyethyleneimine (BPEI-L) as a core and used for gene delivery. The obtained polymers display low toxicity and efficient gene delivery at low nitrogen-to-phosphate (N/P) ratios. Using successive attachment of ethyleneimine moieties to a PEI core, the polymer has a lower relative ratio of linear-to-branched structures than in the core PEI. The more extensive branching enables the polymer to condense plasmid DNA into nanostructure complexes with a size of less than or equal to about 100 nm. The complexes are stable and efficient in transfecting cells in the presence of serum. Bioluminescent imaging of in vivo gene expression using a luciferase reporter gene performed in live mice showed gene expression in the liver and in submandibular lymph nodes.
摘要翻译: 使用低分子量聚乙烯亚胺(BPEI-L)作为核心并用于基因递送合成超支化树突(HD)聚合物。 所获得的聚合物在低氮磷酸盐(N / P)比低的情况下显示低毒性和有效的基因递送。 使用乙烯亚胺部分连续连接到PEI芯上,该聚合物具有比在芯PEI中更低的线性至支化结构的相对比例。 更广泛的分支使得聚合物能够将质粒DNA冷凝成尺寸小于或等于约100nm的纳米结构复合物。 复合物在血清存在下转染细胞是稳定和有效的。 使用在活鼠中进行的荧光素酶报告基因的体内基因表达的生物发光成像显示在肝脏和下颌下淋巴结中的基因表达。
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公开(公告)号:US06737247B2
公开(公告)日:2004-05-18
申请号:US09999665
申请日:2001-10-19
申请人: Alexei Bogdanov , Ralph Weissleder
发明人: Alexei Bogdanov , Ralph Weissleder
IPC分类号: C12Q126
CPC分类号: G01R33/48 , G01R33/465
摘要: The invention features methods of detecting enzymatic activity (e.g., in a magnetic resonance image). In general, the methods include: (1) providing a monomeric substrate (e.g., a substrate that is polymerizable in the presence of an enzyme or as a result of an enzyme-catalyzed reaction), having the generic structure X-Y-Z, where X includes a chelator moiety having a chelated paramagnetic or superparamagnetic metal atom or ion, Y includes a linker moiety (e.g., to provide a covalent or non-covalent chemical bond or bonds between X and Z), and Z includes a polymerizing moiety; (2) contacting the substrate with a target tissue, wherein the substrate undergoes polymerization to form a paramagnetic or superparamagnetic polymer, the polymerization being catalyzed by an enzyme in an extracellular matrix or bound to the surfaces of cells of the target tissue; and (3) detecting an increase in relaxivity for the polymer relative to an equivalent amount of unpolymerized substrate. The invention also features substrate compositions.
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公开(公告)号:US06592847B1
公开(公告)日:2003-07-15
申请号:US09604145
申请日:2000-06-27
IPC分类号: A61B1000
CPC分类号: A61K49/0056 , A61B5/0068 , A61B5/0071 , A61B5/0084 , A61B5/0086 , A61K47/60 , A61K49/0032 , A61K49/0054 , A61K49/0058 , C12Q1/6841 , C12Q1/6883 , C12Q2565/30 , C12Q2565/1015 , C12Q2525/301
摘要: An intramolecularly-quenched, near infrared fluorescence probe that emits substantial fluorescence only after interaction with a target tissue (i.e., activation) is disclosed. The probe includes a polymeric backbone and a plurality of near infrared fluorochromes covalently linked to the backbone at fluorescence-quenching interaction-permissive positions separable by enzymatic cleavage at fluorescence activation sites. The probe optionally includes protective chains or fluorochrome spacers, or both. Also disclosed are methods of using the intramolecularly-quenched, near infrared fluorescence probes for in vivo optical imaging.
摘要翻译: 公开了分子内骤冷的近红外荧光探针,其仅在与靶组织相互作用后发射实质性荧光(即,激活)。 探针包括聚合物主链和多个近红外荧光染料,其在荧光猝灭相互作用下共价连接至主链,允许位置可通过荧光激活位点处的酶切割分离。 探针任选地包括保护链或荧光染料间隔物,或两者。 还公开了使用分子内熄灭的近红外荧光探针进行体内光学成像的方法。
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公开(公告)号:US08153784B2
公开(公告)日:2012-04-10
申请号:US11631720
申请日:2005-07-07
申请人: Alexei Bogdanov , John W. Chen , Ralph Weissleder , Manel Querol
发明人: Alexei Bogdanov , John W. Chen , Ralph Weissleder , Manel Querol
IPC分类号: C07D225/00 , C07D245/00
CPC分类号: G01N33/5088 , G01N33/5014 , G01N33/5067
摘要: This invention relates to biochemistry and magnetic resonance imaging.
摘要翻译: 本发明涉及生物化学和磁共振成像。
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