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公开(公告)号：US5736374A
公开(公告)日：1998-04-07
申请号：US596985
申请日：1996-02-05
申请人： Randy M. Berka , John A. Hucul , Michael Ward
发明人： Randy M. Berka , John A. Hucul , Michael Ward
CPC分类号： C12Y302/01023 , C12N9/2471
摘要： Novel methods are disclosed for the enhanced expression and secretion of lactase from filamentous fungi. Specifically the novel processes cause enhanced production of lactase from an Aspergillus and preferably enhanced production of A. oryzae lactase from A. oryzae host strains transformed with necessary DNA. Also described are the DNA sequence encoding the lactase gene from A. oryzae and the deduced amino acid sequence of the lactase therefrom.
摘要翻译： 公开了从丝状真菌增强乳糖酶的表达和分泌的新方法。 具体地，新方法导致来自曲霉的乳糖酶的产生增加，并且优选从用必需DNA转化的米曲霉宿主菌株中增加米曲霉乳糖酶的产生。 还描述了编码米曲霉的乳糖酶基因的DNA序列和由此推断的乳糖酶的氨基酸序列。
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公开(公告)号：US06509171B1
公开(公告)日：2003-01-21
申请号：US09048473
申请日：1998-03-26
IPC分类号： C12P2102
CPC分类号： C12N9/6481 , A23C19/04 , C12N9/62 , C12N15/80 , C12R1/665
摘要： This invention relates to novel mutant filamentous fungi which are deficient in the gene for the corresponding aspartic proteinase. These organisms are useful production hosts in the production of heterologous polypeptides such as chymosin.
摘要翻译： 本发明涉及缺乏相应天冬氨酸蛋白酶基因的新型突变丝状真菌。 这些生物体是生产异源多肽如凝乳酶的有用生产宿主。
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公开(公告)号：US5840570A
公开(公告)日：1998-11-24
申请号：US345018
申请日：1994-11-23
CPC分类号： C12N9/6481 , A23C19/04 , C12N15/80 , C12N9/62 , C12R1/665
摘要： This invention relates to novel mutant filamentous fungi which are deficient in the gene for the corresponding aspartic proteinase. These organisms are useful production hosts in the production of heterologous polypeptides such as chymosin.
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公开(公告)号：US08362221B2
公开(公告)日：2013-01-29
申请号：US13421682
申请日：2012-03-15
IPC分类号： C07H21/02
CPC分类号： C12Q1/689 , C12Q1/6809 , C12Q2600/158 , C12Q2565/501 , C12Q2565/102
摘要： The present invention relates to methods for monitoring differential expression of a plurality of genes in a first Bacillus cell relative to expression of the same genes in one or more second Bacillus cells using microarrays containing Bacillus genomic sequenced tags. The present invention also relates to computer readable media and computer-based systems. The present invention further relates to substrates containing an array of Bacillus licheniformis or Bacillus clausii GSTs.
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公开(公告)号：US08168773B2
公开(公告)日：2012-05-01
申请号：US12753000
申请日：2010-04-01
CPC分类号： C12Q1/689 , C12Q1/6809 , C12Q2600/158 , C12Q2565/501 , C12Q2565/102
摘要： The present invention relates to methods for monitoring differential expression of a plurality of genes in a first Bacillus cell relative to expression of the same genes in one or more second Bacillus cells using microarrays containing Bacillus genomic sequenced tags. The present invention also relates to computer readable media and computer-based systems. The present invention further relates to substrates containing an array of Bacillus licheniformis or Bacillus clausii GSTs.
摘要翻译： 本发明涉及使用含有芽孢杆菌基因组测序标签的微阵列来监测第一个芽孢杆菌细胞中多个基因的差异表达相对于一个或多个第二个芽孢杆菌细胞中相同基因表达的差异表达的方法。 本发明还涉及计算机可读介质和基于计算机的系统。 本发明还涉及含有地衣芽孢杆菌或克劳氏杆菌GST阵列的底物。
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公开(公告)号：US06221644B1
公开(公告)日：2001-04-24
申请号：US09163642
申请日：1998-09-30
申请人： Randy M. Berka , Michael W. Rey , Alan V. Klotz
发明人： Randy M. Berka , Michael W. Rey , Alan V. Klotz
IPC分类号： C12N916
CPC分类号： C12Y301/03008 , A23K20/189 , A23L5/25 , A23L11/33 , A23L29/35 , C12N9/16 , C12Y301/03026
摘要： The present invention relates to isolated polypeptides having phytase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the polypeptides. The present invention further relates to composite feeds and methods of reducing phytate levels.
摘要翻译： 本发明涉及具有植酸酶活性的分离多肽和分离的编码多肽的核酸序列。 本发明还涉及包含核酸序列的核酸构建体，载体和宿主细胞以及用于产生多肽的方法。 本发明还涉及复合饲料和降低植酸盐含量的方法。
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7.发明授权Haloperoxidases from Curvularia verruculosa and nucleic acids encoding same 失效来自曲霉疣孢菌的卤素过氧化物酶和编码相同的核酸
公开(公告)号：US5965418A
公开(公告)日：1999-10-12
申请号：US842799
申请日：1997-04-16
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/02 , C12N9/08 , C12N15/52 , C12N15/53 , C12N15/80 , C12P21/02 , C12R1/645 , C12N15/63
CPC分类号： C12N9/0065 , Y10S435/911
摘要： The present invention relates to Curvularia verruculosa haloperoxidases and isolated nucleic acid fragments comprising nucleic acid sequences encoding the haloperoxidases as well as nucleic acid constructs, vectors, and recombinant host cells comprising the nucleic acid sequences. The invention also relates to methods for recombinant production of the haloperoxidase. The invention further relates to compositions comprising the haloperoxidases and methods of using the compositions for killing microbial cells or inhibiting growth of microbial cells.
摘要翻译： 本发明涉及疣状弧菌弧菌过氧化物酶和包含编码卤代过氧化物酶的核酸序列以及包含核酸序列的核酸构建体，载体和重组宿主细胞的分离的核酸片段。 本发明还涉及重组生产卤代过氧化物酶的方法。 本发明还涉及包含卤代过氧化物酶的组合物和使用该组合物杀死微生物细胞或抑制微生物细胞生长的方法。
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公开(公告)号：US5853702A
公开(公告)日：1998-12-29
申请号：US797366
申请日：1997-02-07
IPC分类号： C12N15/09 , C07K19/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N9/24 , C12N15/00 , C12N15/55 , C12R1/07 , C12R1/46 , C12R1/465 , C12R1/645 , C12R1/66 , C12R1/72 , C12R1/77 , C12R1/785 , C12R1/80 , C12R1/84 , C12R1/85 , A61K7/28 , C07H21/04 , C07K1/00
CPC分类号： C12Y302/01084 , C12N9/2402 , Y10S435/933 , Y10S435/935
摘要： The present invention relates to polypeptides having mutanase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the polypeptides. The present invention further relates to oral cavity compositions and methods for degrading mutan.
摘要翻译： 本发明涉及具有衍生葡聚糖酶活性的多肽和分离的编码多肽的核酸序列。 本发明还涉及包含核酸序列的核酸构建体，载体和宿主细胞以及用于产生多肽的方法。 本发明还涉及口腔组合物和降解变应原的方法。
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公开(公告)号：US5821102A
公开(公告)日：1998-10-13
申请号：US784651
申请日：1997-01-21
CPC分类号： C12N9/20
摘要： The present invention relates to isolated nucleic acid sequences encoding polypeptides having Absidia lipase activity. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the polypeptides. The invention further relates to compositions comprising the polypeptides.
摘要翻译： 本发明涉及编码具有唾液脂肪酶活性的多肽的分离的核酸序列。 本发明还涉及包含核酸序列的核酸构建体，载体和宿主细胞以及用于产生多肽的方法。 本发明还涉及包含该多肽的组合物。
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公开(公告)号：US5578463A
公开(公告)日：1996-11-26
申请号：US284942
申请日：1994-08-02
IPC分类号： C12N1/15 , C12N1/19 , C12N9/34 , C12N9/58 , C12N9/64 , C12N15/62 , C12N15/80 , C12N15/09 , C12N15/56
CPC分类号： C12N9/2428 , C12N15/62 , C12N15/80 , C12N9/58 , C12N9/6481 , C07K2319/00 , C07K2319/02 , C07K2319/50 , C07K2319/61
摘要： Novel vectors are disclosed for expressing and secreting heterologous polypeptides from filamentous fungi. Such vectors are used in novel processes to express and secrete such heterologous polypeptides. The vectors used for transforming a filamentous fungus to express and secrete a heterologous polypeptide include a DNA sequence encoding a heterologous polypeptide and a DNA sequence encoding a signal sequence which is functional in a secretory system in a given filamentous fungus and which is operably linked to the sequence encoding the heterologous polypeptide. Such signal sequences may be the signal sequence normally associated with the heterologous polypeptides or may be derived from other sources. The vector may also contain DNA sequences encoding a promoter sequence which is functionally recognized by the filamentous fungus and which is operably linked to the DNA sequence encoding the signal sequence. Preferably functional polyadenylation sequences are operably linked to the 3' terminus of the DNA sequence encoding the heterologous polypeptides. Each of the above described vectors are used in novel processes to transform a filamentous fungus wherein the DNA sequences encoding the signal sequence and heterologous polypeptide are expressed. The thus synthesized polypeptide is thereafter secreted from the filamentous fungus.
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