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    Covalently attached collagen VI for cell attachment and proliferation
    1.
    发明申请
    Covalently attached collagen VI for cell attachment and proliferation 审中-公开
    共价连接胶原蛋白VI用于细胞附着和增殖

    公开(公告)号：US20060105455A1

    公开(公告)日：2006-05-18

    申请号：US11322637

    申请日：2005-12-30

    申请人： Richard Guarino ,  Jonathan Rowley ,  Andrea Liebmann-Vinson ,  John Hemperly ,  Mohammad Heidaran ,  Sharon Presnell

    发明人： Richard Guarino ,  Jonathan Rowley ,  Andrea Liebmann-Vinson ,  John Hemperly ,  Mohammad Heidaran ,  Sharon Presnell

    IPC分类号： C12N5/08

    CPC分类号： G01N33/54393 ,  C12N5/0068 ,  C12N2500/90 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/80

    摘要： Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, collagen VI or a biologically active fragment or variant thereof and, optionally, one or more other ECM proteins (or fragments or variants thereof) such as elastin, fibronectin, vitronectin, tenascin, laminin, entactin, aggrecan, decorin, collagen I, collagen III, and collagen IV. Also, optionally present on the surface is one or more polycationic polymers, such as poly-D-lysine or poly-D-ornithine. This surface is used in cell culture to promote cell attachment, survival, and/or proliferation of a number of different cell types such as (a) liver cells (e.g., HepG2 tumor cells, and a newly discovered line of rat liver epithelial stem cells) (b) osteoblasts, such as the murine cell line MC3T3 cell line and (c) primary bone marrow cells. Kits comprising the surfaces and additional reagents are also disclosed.

    摘要翻译： 用于细胞培养物的表面包括与CAR材料结合的载体，并且结合CAR材料，胶原VI或其生物活性片段或变体，以及任选的一种或多种其它ECM蛋白（或其片段或变体） ），例如弹性蛋白，纤连蛋白，玻连蛋白，腱生蛋白，层粘连蛋白，内含子，聚集蛋白聚糖，核心蛋白聚糖，胶原I，胶原III和胶原IV。 此外，任选存在于表面上的是一种或多种聚阳离子聚合物，例如聚-D-赖氨酸或聚-D-鸟氨酸。 该表面用于细胞培养以促进许多不同细胞类型的细胞附着，存活和/或增殖，例如（a）肝细胞（例如，HepG2肿瘤细胞和新发现的大鼠肝上皮干细胞系 ）（b）成骨细胞，例如鼠细胞系MC3T3细胞系和（c）原代骨髓细胞。 还公开了包含表面和附加试剂的试剂盒。

    Covalently attached collagen VI for cell attachment and proliferation
    2.
    发明申请
    Covalently attached collagen VI for cell attachment and proliferation 审中-公开
    共价连接胶原蛋白VI用于细胞附着和增殖

    公开(公告)号：US20050058687A1

    公开(公告)日：2005-03-17

    申请号：US10660781

    申请日：2003-09-12

    申请人： Richard Guarino ,  Jonathan Rowley ,  Andrea Liebmann-Vinson ,  John Hemperly ,  Mohammad Heidaran ,  Sharon Presnell

    发明人： Richard Guarino ,  Jonathan Rowley ,  Andrea Liebmann-Vinson ,  John Hemperly ,  Mohammad Heidaran ,  Sharon Presnell

    IPC分类号： C12N5/00 ,  C12N5/02 ,  C12N5/08 ,  G01N33/543

    CPC分类号： G01N33/54393 ,  C12N5/0068 ,  C12N2500/90 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/80

    摘要： Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, collagen VI or a biologically active fragment or variant thereof and, optionally, one or more other ECM proteins (or fragments or variants thereof) such as elastin, fibronectin, vitronectin, tenascin, laminin, entactin, aggrecan, decorin, collagen I, collagen III, and collagen IV. Also, optionally present on the surface is one or more polycationic polymers, such as poly-D-lysine or poly-D-ornithine. This surface is used in cell culture to promote cell attachment, survival, and/or proliferation of a number of different cell types such as (a) liver cells (e.g., HepG2 tumor cells, and a newly discovered line of rat liver epithelial stem cells) (b) osteoblasts, such as the murine cell line MC3T3 cell line and (c) primary bone marrow cells. Kits comprising the surfaces and additional reagents are also disclosed.

    摘要翻译： 用于细胞培养物的表面包括与CAR材料结合的载体，并结合CAR材料，胶原VI或其生物活性片段或变体，以及任选的一种或多种其它ECM蛋白（或其片段或变体） ），例如弹性蛋白，纤连蛋白，玻连蛋白，腱生蛋白，层粘连蛋白，内含子，聚集蛋白聚糖，核心蛋白聚糖，胶原I，胶原III和胶原IV。 此外，任选存在于表面上的是一种或多种聚阳离子聚合物，例如聚-D-赖氨酸或聚-D-鸟氨酸。 该表面用于细胞培养以促进许多不同细胞类型的细胞附着，存活和/或增殖，例如（a）肝细胞（例如，HepG2肿瘤细胞和新发现的大鼠肝上皮干细胞系 ）（b）成骨细胞，例如鼠细胞系MC3T3细胞系和（c）原代骨髓细胞。 还公开了包含表面和附加试剂的试剂盒。

    Environments that maintain function of primary liver cells
    3.
    发明申请
    Environments that maintain function of primary liver cells 审中-公开
    维持原发性肝细胞功能的环境

    公开(公告)号：US20050059150A1

    公开(公告)日：2005-03-17

    申请号：US10664037

    申请日：2003-09-17

    申请人： Richard Guarino ,  Sharon Presnell ,  Andrea Liebmann-Vinson ,  Mohammad Heidaran

    发明人： Richard Guarino ,  Sharon Presnell ,  Andrea Liebmann-Vinson ,  Mohammad Heidaran

    IPC分类号： C12N5/071 ,  G01N33/50 ,  C12N5/08

    CPC分类号： G01N33/5067 ,  C12N5/067 ,  C12N5/0671 ,  C12N2533/32 ,  C12N2533/54

    摘要： Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, an ECM protein, or a biologically active fragment or variant thereof such as elastin, fibronectin, vitronectin, laminin, collagen I, collagen III, collagen IV, and collagen VI. Also, optionally present on the surface is an active factor, preferably a polycationic polymer or a biologically active fragment or variant thereof, such as polyethyleneimine (PEI), poly-D-lysine (PDL), poly-L-lysine (PLL), poly-D-ornithine (PDO) or poly-L-ornithine (PLO). This surface is used in cell culture to promote cell attachment, survival, and/or proliferation of primary liver cells. The invention also relates to methods utilizing this surface, such as methods for attachment, survival, and/or proliferation of cells. Further disclosed is the use of the surface in cell culture with serum-free medium. Methods of screening using the surface of the invention are also disclosed.

    摘要翻译： 用于细胞培养的表面包括与CAR材料结合的载体，并结合CAR材料，ECM蛋白或其生物活性片段或变体如弹性蛋白，纤连蛋白，玻连蛋白，层粘连蛋白，胶原I，胶原 III，胶原IV和胶原蛋白VI。 此外，表面上任选存在的是活性因子，优选聚阳离子聚合物或其生物活性片段或变体，例如聚乙烯亚胺（PEI），聚-D-赖氨酸（PDL），聚-L-赖氨酸（PLL）， 聚-D-鸟氨酸（PDO）或聚-L-鸟氨酸（PLO）。 该表面用于细胞培养以促进原代肝细胞的细胞附着，存活和/或增殖。 本发明还涉及利用该表面的方法，例如细胞附着，存活和/或增殖的方法。 还公开了使用无血清培养基的细胞培养物中的表面。 还公开了使用本发明的表面进行筛选的方法。

    Environments that maintain function of primary liver cells
    6.
    发明申请
    Environments that maintain function of primary liver cells 审中-公开
    维持原发性肝细胞功能的环境

    公开(公告)号：US20070092861A1

    公开(公告)日：2007-04-26

    申请号：US10570702

    申请日：2004-09-10

    申请人： Richard Guarino ,  Sharon Presnell ,  Andrea Liebmann-Vinson ,  Mohammed Heidaran ,  John Hemperly ,  Stewart Jurgensen ,  Ronald Laethem ,  Neil Robbins

    发明人： Richard Guarino ,  Sharon Presnell ,  Andrea Liebmann-Vinson ,  Mohammed Heidaran ,  John Hemperly ,  Stewart Jurgensen ,  Ronald Laethem ,  Neil Robbins

    IPC分类号： C12Q1/00 ,  C12N5/08

    CPC分类号： G01N33/5067 ,  C12N5/067 ,  C12N5/0671 ,  C12N2533/32 ,  C12N2533/54

    摘要： Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, an ECM protein, or a biologically active fragment or variant thereof such as elastin, fibronectin, vitronectin, lamninin, collagen I, collagen III, collagen IV, and collagen VI. Also, optionally present on the surface is an active factor, preferably a polycationic polymer or a biologically active fragment or variant thereof, such as polyethyleneimine (PEI), poly-D-lysine (PDL), poly-L-lysine (PLL), poly-D-ornithine (PDO) or poly-L-ornithine (PLO). This surface is used in cell culture to promote cell attachment, survival, maintenance of function, and/or proliferation of primary liver cells. The invention also relates to methods utilizing this surface, such as methods for attachment, survival, maintenance of function, and/or proliferation of cells. Further disclosed is the use of the surface in cell culture with serum-free medium. Methods of screening using the surface of the invention are also disclosed.

    摘要翻译： 用于细胞培养的表面包括与CAR材料结合的载体，并结合CAR材料，ECM蛋白或其生物活性片段或变体如弹性蛋白，纤连蛋白，玻连蛋白，lamninin，胶原I，胶原 III，胶原IV和胶原蛋白VI。 此外，表面上任选存在的是活性因子，优选聚阳离子聚合物或其生物活性片段或变体，例如聚乙烯亚胺（PEI），聚-D-赖氨酸（PDL），聚-L-赖氨酸（PLL）， 聚-D-鸟氨酸（PDO）或聚-L-鸟氨酸（PLO）。 该表面用于细胞培养以促进细胞附着，存活，功能的维持和/或原代肝细胞的增殖。 本发明还涉及利用该表面的方法，例如附着，存活，维持功能和/或细胞增殖的方法。 还公开了使用无血清培养基的细胞培养物中的表面。 还公开了使用本发明的表面进行筛选的方法。

    High throughput method to identify ligands for cell attachment
    8.
    发明申请
    High throughput method to identify ligands for cell attachment 审中-公开
    用于鉴定细胞附着配体的高通量方法

    公开(公告)号：US20050059083A1

    公开(公告)日：2005-03-17

    申请号：US10662640

    申请日：2003-09-15

    申请人： Andrea Liebmann-Vinson ,  Jonathan Rowley ,  Chris Bodily ,  Mohammad Heidaran

    发明人： Andrea Liebmann-Vinson ,  Jonathan Rowley ,  Chris Bodily ,  Mohammad Heidaran

    IPC分类号： G01N33/50 ,  G01N33/543 ,  G01N33/53

    CPC分类号： G01N33/5011 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/5029 ,  G01N33/54393

    摘要： A high throughput method is provided for identifying agents capable of producing a desired biological response in whole cells. The method includes the steps of providing receptacles having a culture surface; placing different mixtures of single agents into selective ones of the receptacles according to a statistical design; and immobilizing the mixtures of single agents to the culture surface. The method further includes contacting the immobilized agents with the whole cells; and acquiring data which is indicative of a desired biological response in the contacted cells. The method also includes using statistical modeling of the acquired data to determine which mixtures of single agents and/or which single agents in these mixtures are effective in producing the desired biological response in the contacted cells.

    摘要翻译： 提供了一种高通量方法，用于鉴定能够在全细胞中产生所需生物反应的试剂。 该方法包括提供具有培养表面的容器的步骤; 根据统计设计将单一试剂的不同混合物置于选择性容器中; 并将单一试剂的混合物固定到培养表面。 该方法还包括使固定化的试剂与整个细胞接触; 并且获取指示所接触细胞中所需生物反应的数据。 该方法还包括使用获取的数据的统计建模来确定单个试剂的哪些混合物和/或这些混合物中的哪一种试剂在接触的细胞中产生所需的生物反应是有效的。