公开(公告)号：US07052861B2

公开(公告)日：2006-05-30

申请号：US10007526

申请日：2001-12-05

申请人： Richard J. Massey ,  Mark T. Martin ,  Liwen Dong ,  Ming Lu ,  Alan Fischer ,  Fabian Jameison ,  Pam Liang ,  Robert Hoch ,  Jonathan K. Leland

发明人： Richard J. Massey ,  Mark T. Martin ,  Liwen Dong ,  Ming Lu ,  Alan Fischer ,  Fabian Jameison ,  Pam Liang ,  Robert Hoch ,  Jonathan K. Leland

IPC分类号： G01N33/573 ,  G01N33/533 ,  G01N33/553 ,  G01N21/76 ,  C12Q1/32

CPC分类号： C01B31/0213 ,  B82Y30/00 ,  B82Y40/00 ,  C01B32/152 ,  C01B32/174 ,  C01B2202/34 ,  C01B2202/36 ,  C07K17/06 ,  D01F9/12 ,  D01F11/14 ,  G01N21/69 ,  G01N21/76 ,  G01N33/54373 ,  G01N33/5438 ,  G01N33/582 ,  G01N2030/8863 ,  G11C13/025 ,  H01M4/60 ,  Y10S435/968 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S977/737 ,  Y10S977/742

摘要： Graphitic nanotubes, which include tubular fullerenes (commonly called “buckytubes”) and fibrils, which are functionalized by chemical substitution, are used as solid supports in electrogenerated chemiluminescence assays. The graphitic nanotubes are chemically modified with functional group biomolecules prior to use in an assay. Association of electrochemiluminescent ruthenium complexes with the functional group biomolecule-modified nanotubes permits detection of molecules including nucleic acids, antigens, enzymes, and enzyme substrates by multiple formats.

公开(公告)号：US09505619B2

公开(公告)日：2016-11-29

申请号：US11385277

申请日：2006-03-20

申请人： Richard J. Massey ,  Mark T. Martin ,  Liwen Dong ,  Ming Lu ,  Alan Fischer ,  Fabian Jameison ,  Pam Liang ,  Robert Hoch ,  Jonathan K. Leland

发明人： Richard J. Massey ,  Mark T. Martin ,  Liwen Dong ,  Ming Lu ,  Alan Fischer ,  Fabian Jameison ,  Pam Liang ,  Robert Hoch ,  Jonathan K. Leland

IPC分类号： G01N33/573 ,  C12Q1/32 ,  C01B31/02 ,  B82Y30/00 ,  B82Y40/00 ,  C07K17/06 ,  D01F9/12 ,  D01F11/14 ,  G01N33/543 ,  G01N33/58 ,  H01M4/60 ,  G01N21/69 ,  G01N21/76 ,  G01N30/88 ,  G11C13/02

CPC分类号： C01B31/0213 ,  B82Y30/00 ,  B82Y40/00 ,  C01B32/152 ,  C01B32/174 ,  C01B2202/34 ,  C01B2202/36 ,  C07K17/06 ,  D01F9/12 ,  D01F11/14 ,  G01N21/69 ,  G01N21/76 ,  G01N33/54373 ,  G01N33/5438 ,  G01N33/582 ,  G01N2030/8863 ,  G11C13/025 ,  H01M4/60 ,  Y10S435/968 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S977/737 ,  Y10S977/742

摘要： Graphitic nanotubes, which include tubular fullerenes (commonly called “buckytubes”) and fibrils, which are functionalized by chemical substitution, are used as solid supports in electrogenerated chemiluminescence assays. The graphitic nanotubes are chemically modified with functional group biomolecules prior to use in an assay. Association of electrochemiluminescent ruthenium complexes with the functional group biomolecule-modified nanotubes permits detection of molecules including nucleic acids, antigens, enzymes, and enzyme substrates by multiple formats.

摘要翻译： 石墨纳米管，其包括通过化学取代官能化的管状富勒烯（通常称为“buckytubes”）和原纤维，被用作电致化学发光测定中的固体支持物。 石墨纳米管在用于测定之前用官能团生物分子进行化学修饰。 电化学发光钌络合物与官能团生物分子修饰的纳米管的关联允许通过多种形式检测包括核酸，抗原，酶和酶底物的分子。

公开(公告)号：US06362011B1

公开(公告)日：2002-03-26

申请号：US09243215

申请日：1999-02-02

申请人： Richard J. Massey ,  Mark T. Martin ,  Liwen Dong ,  Ming Lu ,  Alan Fischer ,  Fabian Jameison ,  Pam Liang ,  Robert Hoch ,  Jonathan K. Leland

发明人： Richard J. Massey ,  Mark T. Martin ,  Liwen Dong ,  Ming Lu ,  Alan Fischer ,  Fabian Jameison ,  Pam Liang ,  Robert Hoch ,  Jonathan K. Leland

IPC分类号： G01N33551

CPC分类号： C01B31/0213 ,  B82Y30/00 ,  B82Y40/00 ,  C01B32/152 ,  C01B32/174 ,  C01B2202/34 ,  C01B2202/36 ,  C07K17/06 ,  D01F9/12 ,  D01F11/14 ,  G01N21/69 ,  G01N21/76 ,  G01N33/54373 ,  G01N33/5438 ,  G01N33/582 ,  G01N2030/8863 ,  G11C13/025 ,  H01M4/60 ,  Y10S435/968 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S977/737 ,  Y10S977/742

摘要： Graphitic nanotubes, which include tubular fullerenes (commonly called “buckytubes”) and fibrils, which are functionalized by chemical substitution, are used as solid supports in electrogenerated chemiluminescence assays. The graphitic nanotubes are chemically modified with functional group biomolecules prior to use in an assay. Association of electrochemiluminescent ruthenium complexes with the functional group biomolecule-modified nanotubes permits detection of molecules including nucleic acids, antigens, enzymes, and enzyme substrates by multiple formats.

摘要翻译： 石墨纳米管，其包括通过化学取代官能化的管状富勒烯（通常称为“巴氏管”）和原纤维，被用作电致化学发光测定中的固体支持物。 石墨纳米管在用于测定之前用官能团生物分子进行化学修饰。 电化学发光钌络合物与官能团生物分子修饰的纳米管的关联允许通过多种形式检测包括核酸，抗原，酶和酶底物的分子。

公开(公告)号：US5866434A

公开(公告)日：1999-02-02

申请号：US611347

申请日：1996-03-06

申请人： Richard J. Massey ,  Mark T. Martin ,  Liwen Dong ,  Ming Lu ,  Alan Fischer ,  Fabian Jameison ,  Pam Liang ,  Robert Hoch ,  Jonathan K. Leland

发明人： Richard J. Massey ,  Mark T. Martin ,  Liwen Dong ,  Ming Lu ,  Alan Fischer ,  Fabian Jameison ,  Pam Liang ,  Robert Hoch ,  Jonathan K. Leland

IPC分类号： B01J20/281 ,  A61K47/04 ,  B01J20/20 ,  B01J21/18 ,  B01J32/00 ,  C01B31/02 ,  C07K16/44 ,  C07K17/06 ,  D01F9/12 ,  D01F11/14 ,  G01N21/69 ,  G01N21/76 ,  G01N21/78 ,  G01N30/88 ,  G01N33/543 ,  G01N33/553 ,  G01N33/573 ,  G01N33/58 ,  H01M4/60 ,  G01N33/551 ,  C12Q1/32 ,  C12Q1/37

CPC分类号： C01B31/0213 ,  B82Y30/00 ,  B82Y40/00 ,  C01B31/0273 ,  C07K17/06 ,  D01F11/14 ,  D01F9/12 ,  G01N33/54373 ,  G01N33/5438 ,  G01N33/582 ,  H01M4/60 ,  C01B2202/34 ,  C01B2202/36 ,  G01N2030/8863 ,  G01N21/69 ,  G01N21/76 ,  G11C13/025 ,  Y10S435/968 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S977/737 ,  Y10S977/742

摘要： Graphitic nanotubes, which include tubular fullerenes (commonly called "buckytubes") and fibrils, which are functionalized by chemical substitution, are used as solid supports in electrogenerated chemiluminescence assays. The graphitic nanotubes are chemically modified with functional group biomolecules prior to use in an assay. Association of electrochemiluminescent ruthenium complexes with the functional group biomolecule-modified nanotubes permits detection of molecules including nucleic acids, antigens, enzymes, and enzyme substrates by multiple formats.

公开(公告)号：US20060160246A1

公开(公告)日：2006-07-20

申请号：US11385277

申请日：2006-03-20

申请人： Richard Massey ,  Mark Martin ,  Liwen Dong ,  Ming Lu ,  Alan Fischer ,  Fabian Jameison ,  Pam Liang ,  Robert Hoch ,  Jonathan Leland

发明人： Richard Massey ,  Mark Martin ,  Liwen Dong ,  Ming Lu ,  Alan Fischer ,  Fabian Jameison ,  Pam Liang ,  Robert Hoch ,  Jonathan Leland

IPC分类号： G01N33/543 ,  C12M1/34

CPC分类号： C01B31/0213 ,  B82Y30/00 ,  B82Y40/00 ,  C01B32/152 ,  C01B32/174 ,  C01B2202/34 ,  C01B2202/36 ,  C07K17/06 ,  D01F9/12 ,  D01F11/14 ,  G01N21/69 ,  G01N21/76 ,  G01N33/54373 ,  G01N33/5438 ,  G01N33/582 ,  G01N2030/8863 ,  G11C13/025 ,  H01M4/60 ,  Y10S435/968 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S977/737 ,  Y10S977/742

摘要： Graphitic nanotubes, which include tubular fullerenes (commonly called “buckytubes”) and fibrils, which are functionalized by chemical substitution, are used as solid supports in electrogenerated chemiluminescence assays. The graphitic nanotubes are chemically modified with functional group biomolecules prior to use in an assay. Association of electrochemiluminescent ruthenium complexes with the functional group biomolecule-modified nanotubes permits detection of molecules including nucleic acids, antigens, enzymes, and enzyme substrates by multiple formats.

公开(公告)号：US6165708A

公开(公告)日：2000-12-26

申请号：US880209

申请日：1997-06-23

申请人： Pam Liang ,  Mark T. Martin ,  Liwen Dong

发明人： Pam Liang ,  Mark T. Martin ,  Liwen Dong

IPC分类号： C07D499/26 ,  C12Q1/34 ,  G01N21/78 ,  G01N27/327 ,  G01N27/42 ,  C12Q1/00

CPC分类号： C12Q1/34 ,  G01N2333/986 ,  G01N2415/00 ,  G01N2458/30 ,  G01N2458/40

摘要： Detectable compounds comprising a chemically-transformable first compound covalently linked to an electrochemiluminescent compound are provided. Such compounds are useful in processes and kits that monitor the status of the first compound and derive information from such monitoring.

摘要翻译： 提供了包含与电化学发光化合物共价连接的可化学转化的第一化合物的可检测化合物。 这些化合物可用于监测第一化合物的状态并从这种监测中导出信息的方法和试剂盒。

公开(公告)号：US5643713A

公开(公告)日：1997-07-01

申请号：US485419

申请日：1995-06-07

申请人： Pam Liang ,  Mark T. Martin ,  Liwen Dong

发明人： Pam Liang ,  Mark T. Martin ,  Liwen Dong

IPC分类号： C07D499/26 ,  C12Q1/34 ,  G01N21/78 ,  G01N27/327 ,  G01N27/42 ,  C12Q1/00 ,  G01N33/53 ,  G01N33/554

CPC分类号： C12Q1/34 ,  G01N2333/986 ,  G01N2415/00 ,  G01N2458/30 ,  G01N2458/40

摘要： Detectable compounds comprising a chemically-transformable first compound covalently linked to an electrochemiluminescent compound are provided. Such compounds are useful in processes and kits that monitor the status of the first compound and derive information from such monitoring.

摘要翻译： 提供了包含与电化学发光化合物共价连接的可化学转化的第一化合物的可检测化合物。 这些化合物可用于监测第一化合物的状态并从这种监测中导出信息的方法和试剂盒。

公开(公告)号：US20050181443A1

公开(公告)日：2005-08-18

申请号：US11108840

申请日：2005-04-19

申请人： Ji Sun ,  Pam Liang ,  Mark Martin ,  Liwen Dong

发明人： Ji Sun ,  Pam Liang ,  Mark Martin ,  Liwen Dong

IPC分类号： C07D277/06 ,  C07F15/00 ,  C07K16/44 ,  C12Q1/00 ,  C12Q1/18 ,  C12Q1/34 ,  G01N21/66 ,  G01N21/76 ,  G01N33/533 ,  G01N33/535 ,  G01N33/58 ,  C12Q1/68 ,  C07H21/04 ,  C12M1/34 ,  G01N33/53

CPC分类号： C07F15/0053 ,  C07D277/06 ,  C07K16/44 ,  C12Q1/005 ,  C12Q1/18 ,  C12Q1/34 ,  G01N21/66 ,  G01N21/76 ,  G01N33/533 ,  G01N33/535 ,  G01N33/582 ,  G01N2333/986 ,  G01N2415/00 ,  G01N2458/30 ,  G01N2458/40

摘要： A method of generating a electrochemiluminescent emission, which comprises exposing an electrochemiluminescent label linked to a coreactant, to conditions suitable for inducing electrochemiluminescence; said compound; a system for generating an electrochemiluminescent emission, which comprises said compound, means for exposing said compound to electrochemical energy, and means for detecting or measuring luminescence emitted from said compound or a composition containing same; and a kit for performing an assay using said compound.

摘要翻译： 一种产生电化学发光发射的方法，其包括将与共反应物连接的电化学发光标记物暴露于适于诱导电化学发光的条件下; 说化合物 一种用于产生电化学发光发射的系统，其包括所述化合物，用于将所述化合物暴露于电化学能的装置，以及用于检测或测量从所述化合物或含有它的组合物发射的发光的装置; 以及用于使用所述化合物进行测定的试剂盒。

公开(公告)号：US06524865B1

公开(公告)日：2003-02-25

申请号：US08928075

申请日：1997-09-11

申请人： Mark T. Martin ,  Rick Saul ,  Pam Liang

发明人： Mark T. Martin ,  Rick Saul ,  Pam Liang

IPC分类号： G01N2176

CPC分类号： G01N33/535 ,  C07F15/0053 ,  C07K16/44 ,  G01N33/533

摘要： Electrochemiluminescent-labels and enzyme substrates, which preferably are conjugated, are used in immunoassays and electrochemiluminescence is generated catalytically. In conventional electrochemiluminescence immunoassays, an anti-analyte antibody molecule can give rise to typically 6-8 electrochemiluminescence-active ruthenium atoms, while in the present invention, each enzyme-labeled anti-analyte molecule can give rise to thousands of electrochemiluminescence-active ruthenium atoms per second. An exemplary immunoassay is based on a catalytic process employing &bgr;-lactamase-conjugated anti-analytes which enzymatically hydrolyze electrochemiluminescent-labeled substrates, making them strongly electrochemiluminescent. The electrochemiluminescence signal generated by each anti-analyte molecule (i.e., each analyte molecule) is much greater than with the conventional method. Accordingly, greater sensitivity can be gained in the measurement of low concentrations of a given immunoassay analyte.

摘要翻译： 优选结合的电致化学发光标记和酶底物用于免疫测定，并且催化产生电化学发光。 在常规的电化学发光免疫测定中，抗分析物抗体分子可以产生典型的6-8个电化学发光 - 活性钌原子，而在本发明中，每个酶标记的抗分析物分子可产生数千个电化学发光 - 活性钌原子 每秒。 示例性的免疫测定是基于使用β-内酰胺酶缀合的抗分析物的催化方法，其中酶促水解电化学发光标记的底物，使其具有强电化学发光的功能。 由每个抗分析物分子（即每个分析物分子）产生的电化学发光信号比常规方法大得多。 因此，在给定免疫测定分析物的低浓度的测量中可以获得更高的灵敏度。

公开(公告)号：US07018802B2

公开(公告)日：2006-03-28

申请号：US10234874

申请日：2002-09-04

申请人： Mark T. Martin ,  Rick Saul ,  Pam Liang

发明人： Mark T. Martin ,  Rick Saul ,  Pam Liang

IPC分类号： G01N33/53 ,  C12Q1/34 ,  C12N9/86 ,  C07D499/00

CPC分类号： G01N33/535 ,  C07F15/0053 ,  C07K16/44 ,  G01N33/533

摘要： Electrochemiluminescent-labels and enzyme substrates, which preferably are conjugated, are used in immunoassays and electrochemiluminescence is generated catalytically. In conventional electrochemiluminescence immunoassays, an anti-analyte antibody molecule can give rise to typically 6–8 electrochemiluminescence-active ruthenium atoms, while in the present invention, each enzyme-labeled anti-analyte molecule can give rise to thousands of electrochemiluminescence-active ruthenium atoms per second. An exemplary immunoassay is based on a catalytic process employing β-lactamase-conjugated anti-analytes which enzymatically hydrolyze electrochemiluminescent-labeled substrates, making them strongly electrochemiluminescent. The electrochemiluminescence signal generated by each anti-analyte molecule (i.e., each analyte molecule) is much greater than with the conventional method. Accordingly, greater sensitivity can be gained in the measurement of low concentrations of a given immunoassay analyte.