Electrochemiluminescent assay
    9.
    发明授权
    Electrochemiluminescent assay 失效
    电化学发光测定

    公开(公告)号:US5804400A

    公开(公告)日:1998-09-08

    申请号:US596830

    申请日:1996-02-05

    摘要: A rapid single step assay suitable for the detection or quantification of enzymes, in particular, hydrolases, especially, aminopeptidases and esterases. The enzymatic reaction causes the cleavage of a metal ligand labelled hydrolase substrate. The cleaved ligand alters the electrochemiluminescence of bidentate aromatic heterocyclic nitrogen-containing ligand reagent. The change in electrochemiluminescence correlates to the presence of hydrolase activity present in the sample. The assay can be performed on an IGEN Origen.RTM. Analyzer.

    摘要翻译: 适用于酶,特别是水解酶,特别是氨基肽酶和酯酶的检测或定量的快速单步测定。 酶反应导致金属配体标记的水解酶底物的裂解。 裂解的配体改变二齿芳族杂环含氮配体试剂的电化学发光。 电化学发光的变化与样品中存在的水解酶活性的存在相关。 该测定可以在IGEN Origen TM分析仪上进行。