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    Electrochemical sensor containing an enzyme linked to binding molecules bound to a noble metal surface
    1.
    发明授权
    Electrochemical sensor containing an enzyme linked to binding molecules bound to a noble metal surface 失效
    含有与结合到贵金属表面的结合分子相连的酶的电化学传感器

    公开(公告)号：US5834224A

    公开(公告)日：1998-11-10

    申请号：US519300

    申请日：1995-08-23

    申请人： Petra Ruger ,  Dorothee Ambrosius ,  Bernd Schmidt ,  Peter Sluka ,  Hans-Joachim Guder ,  Erhard Kopetzki

    发明人： Petra Ruger ,  Dorothee Ambrosius ,  Bernd Schmidt ,  Peter Sluka ,  Hans-Joachim Guder ,  Erhard Kopetzki

    IPC分类号： G01N27/327 ,  C07C323/58 ,  C07C323/60 ,  C07D207/323 ,  C07D207/44 ,  C07D207/452 ,  C07D403/12 ,  C07D495/04 ,  C12Q1/00 ,  G01N33/53 ,  G01N33/543 ,  G01N37/00 ,  C12Q1/54 ,  C12M1/34 ,  C12N11/14

    CPC分类号： C07D207/452 ,  B82Y15/00 ,  B82Y30/00 ,  C07C323/58 ,  C12Q1/003 ,  G01N33/54353 ,  Y10S435/817

    摘要： An electrochemical sensor is provided containing a supporting material having a surface of noble metal such as gold or palladium to which is bound an enzyme such as glucose oxidase via binding molecules. The binding molecules are in the form of a homogenous monolayer and are bound to the metal surface by anchor groups such as thiol, disulphide or phosphine groups. The enzyme is linked to the binding molecules by an ionic bond, a covalent bond or a metal-chelate bond. The binding molecules may contain a spacer such as an alkylene chain. Coverage of the binding molecules on the metal surface is less than the maximum coverage to optimize enzyme coverage, decrease response time of the sensor and increase signal yield per surface area. The monolayer may contain diluent molecules which are not linked to an enzyme and which are bound to the metal surface via anchor groups. The diluent molecules lower the concentration of binding molecules in the monolayer, increase conductivity of the monolayer and lower unspecific binding of components from a sample solution in contact with the sensor. The binding molecules can be linked by an ionic bond or a metal-chelate bond, respectively, to a charged amino acid residue or a chelate-forming amino acid residue of the N-terminus, C-terminus, or N- and C-terminus of recombinant enzyme protein.

    摘要翻译： 提供了一种电化学传感器，其包含具有贵金属如金或钯的表面的支撑材料，其通过结合分子结合酶如葡萄糖氧化酶。 结合分子是均匀单层的形式，并通过锚定基团例如硫醇，二硫键或膦基与金属表面结合。 酶通过离子键，共价键或金属螯合键与结合分子连接。 结合分子可以含有间隔基如亚烷基链。 金属表面上的结合分子的覆盖率小于最大覆盖范围，以优化酶覆盖，减少传感器的响应时间并增加每个表面积的信号产量。 单层可以含有不与酶连接并且通过锚定基团结合到金属表面的稀释剂分子。 稀释剂分子降低单层中结合分子的浓度，增加单层的电导率，并降低组件与传感器接触的样品溶液的非特异性结合。 结合分子可以分别通过离子键或金属 - 螯合键连接到带电荷的氨基酸残基或N-末端，C-末端或N-和C-末端的螯合形成氨基酸残基 的重组酶蛋白。

    Agent for the detection of substances with hydrolase activity
    2.
    发明授权
    Agent for the detection of substances with hydrolase activity 失效
    用于检测具有水解酶活性的物质的试剂

    公开(公告)号：US5260428A

    公开(公告)日：1993-11-09

    申请号：US988975

    申请日：1993-01-29

    申请人： Rupert Herrmann ,  Hans-Joachim Guder ,  Werner Guthlein ,  Manfred Kuhr ,  Johann Berger ,  Harvey Buck

    发明人： Rupert Herrmann ,  Hans-Joachim Guder ,  Werner Guthlein ,  Manfred Kuhr ,  Johann Berger ,  Harvey Buck

    IPC分类号： C12Q1/34 ,  G01N33/58 ,  C07H15/24 ,  C07C43/00 ,  C07C63/36 ,  C07C303/00 ,  C07H15/00

    CPC分类号： C12Q1/34 ,  G01N33/581 ,  C12Q2334/30 ,  G01N2333/924

    摘要： A process for the detection of substances with hydrolase activity in a sample by mixing the sample with a hydrolase substrate and an oxidising agent, and the evaluating the resultant colour intensity, wherein, as hydrolase substrate, there is used at least one compound of the formula ##STR1## in which R.sup.1 is hydrogen or an alkoxy radical,R.sup.2 is hydrogen or halogen, an amino group or an alkoxy or aralkoxy radical,R.sup.3, R.sup.4, R.sup.5, R.sup.6, which can be the same or different, are hydrogen, halogen, carboxyl, a carbamoyl group, sulpho, an amino groups alkyl, an alkoxy radical, aralkoxy, alkylcarbonyl or alkoxycarbonyl, andX is a glycosyl, phosphate or acyl residue. Optionally R.sup.2 and R.sup.3, R.sup.3 and R.sup.4, R.sup.4 and R.sup.5, and R.sup.5 and R.sup.6 can be joined to form a ring system. At least one of R.sup.1 and R.sup.2 must be hydrogen and at least one of R.sup.1, R.sup.2 and R.sup.3 must not be hydrogen. Compounds in which X is .beta.-galactosyl are new.

    摘要翻译： 一种用于通过将样品与水解酶底物和氧化剂混合来检测样品中水解酶活性的物质并评估所得颜色强度的方法，其中作为水解酶底物，使用至少一种式 其中R 1是氢或烷氧基，R 2是氢或卤素，氨基或烷氧基或芳烷氧基，可以相同或不同的R 3，R 4，R 5，R 6是氢，卤素， 羧基，氨基甲酰基，磺基，氨基烷基，烷氧基，芳烷氧基，烷基羰基或烷氧基羰基，X是糖基，磷酸酯或酰基残基。 任选地，R 2和R 3，R 3和R 4，R 4和R 5以及R 5和R 6可以连接形成环系。 R 1和R 2中的至少一个必须是氢，R 1，R 2和R 3中的至少一个不能是氢。 X是β-半乳糖基的化合物是新的。

    Process for the detection of substances with hydrolase activity
    5.
    发明授权
    Process for the detection of substances with hydrolase activity 失效
    检测具有水解酶活性的物质的方法

    公开(公告)号：US5202233A

    公开(公告)日：1993-04-13

    申请号：US816795

    申请日：1992-01-03

    申请人： Rupert Herrmann ,  Hans-Joachim Guder ,  Werner Guthlein ,  Manfred Kuhr ,  Johann Berger ,  Harvey Buck

    发明人： Rupert Herrmann ,  Hans-Joachim Guder ,  Werner Guthlein ,  Manfred Kuhr ,  Johann Berger ,  Harvey Buck

    IPC分类号： C12Q1/34 ,  G01N33/58

    CPC分类号： C12Q1/34 ,  G01N33/581 ,  C12Q2334/30 ,  G01N2333/924 ,  Y10S435/805

    摘要： A process for the detection of substances with hydrolase activity in a sample by mixing the sample with a hydrolase substrate and an oxidizing agent, and the evaluating the resultant color intensity, wherein, as hydrolase substrate, there is used at least one compound of the formula ##STR1## in which R.sup.1 is hydrogen or an alkoxy radical,R.sup.2 is hydrogen or halogen, an amino group or an alkoxy or aralkoxy radical,R.sup.3, R.sup.4, R.sup.5 and R.sup.6, which can be the same or different, are hydrogen, halogen, carboxyl, a carbamoyl group, sulpho, an amino groups alkyl, an alkoxy radical, aralkoxy, alkylcarbonyl or alkoxycarbonyl, andX is a glycosyl, phosphate or acyl residue.Optionally R.sup.2 and R.sup.3, R.sup.3 and R.sup.4, R.sup.4 and R.sup.5, and R.sup.5 and R.sup.6 can be joined to form a ring system. At least one of R.sup.1 and R.sup.2 must be hydrogen and at least one of R.sup.1, R.sup.2 and R.sup.3 must not be hydrogen. Compounds in which X is .beta.-galactosyl are new.

    摘要翻译： 一种用于通过将样品与水解酶底物和氧化剂混合来检测样品中水解酶活性的物质并评估所得颜色强度的方法，其中作为水解酶底物，使用至少一种式 其中R 1是氢或烷氧基，R 2是氢或卤素，氨基或烷氧基或芳烷氧基，可以相同或不同的R 3，R 4，R 5和R 6是氢，卤素， 羧基，氨基甲酰基，磺基，氨基烷基，烷氧基，芳烷氧基，烷基羰基或烷氧基羰基，X是糖基，磷酸酯或酰基残基。 任选地，R 2和R 3，R 3和R 4，R 4和R 5以及R 5和R 6可以连接形成环系。 R 1和R 2中的至少一个必须是氢，R 1，R 2和R 3中的至少一个不能是氢。 X是β-半乳糖基的化合物是新的。

    New hydrolase substrates
    8.
    发明授权
    New hydrolase substrates 失效
    新的水解酶底物

    公开(公告)号：US4900822A

    公开(公告)日：1990-02-13

    申请号：US135843

    申请日：1987-12-21

    申请人： Herbert von der Eltz ,  Hans-Joachim Guder ,  Klaus Muhlegger

    发明人： Herbert von der Eltz ,  Hans-Joachim Guder ,  Klaus Muhlegger

    IPC分类号： C07D265/38 ,  C07F9/6533 ,  C07H17/00 ,  C07H21/00 ,  C09B19/00 ,  C09B57/00 ,  C12Q1/34

    CPC分类号： C07H21/00 ,  C07D265/38 ,  C07F9/65335 ,  C07H17/00 ,  C12Q1/34 ,  C12Q2304/22 ,  C12Q2334/00

    摘要： Reagents for the determination of hydrolase comprises compounds of the formula ##STR1## wherein Z is an organic or inorganic acid residue or a sugar residue, A is an organic or inorganic acid residue, and B is an organic acid residue. Each of R.sup.2 and R.sup.5, is hydrogen, halogen or lower alkyl. Each of R.sup.1, R.sup.3, R.sup.4 and R.sup.6, is hydrogen, halogen, cyano, lower alkyl, lower alkoxy, carboxyl, lower alkoxycarbonyl, carboxy lower alkyl or lower alkoxycarbonyl lower alkyl, or carboxamido groups optional substituted once or twice, or a radical of the formula--COO--(CH.sub.2 CH.sub.2 O).sub.n --R.sup.7in which R.sup.7 is hydrogen or lower alkyl and n is a number from 1 to 4. Additionally, R.sup.6 can be sulpho or nitro.

    摘要翻译： 用于测定水解酶的试剂包括下式的化合物：其中Z是有机或无机酸残基或糖残基，A是有机或无机酸残基，B是有机酸残基。 R 2和R 5各自为氢，卤素或低级烷基。 R 1，R 3，R 4和R 6中的每一个为氢，卤素，氰基，低级烷基，低级烷氧基，羧基，低级烷氧基羰基，羧基低级烷基或低级烷氧基羰基低级烷基或任选取代一次或两次的甲酰胺基， 式-COO-（CH 2 CH 2 O）n -R 7，其中R 7是氢或低级烷基，n是1至4的数。另外，R 6可以是磺基或硝基。