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公开(公告)号：US4683205A
公开(公告)日：1987-07-28
申请号：US698254
申请日：1985-02-04
申请人： Ryoichi Katsumata , Akio Ozaki , Tetsuo Oka , Akira Furuya
发明人： Ryoichi Katsumata , Akio Ozaki , Tetsuo Oka , Akira Furuya
摘要： Disclosed is a method for transformation of microorganisms belonging to the genera Corynebacterium and Brevibacterium whereby a foreign DNA may be introduced into a host cell and autonomously replicated.
摘要翻译： 公开了一种用于转化属于棒杆菌属和短杆菌属的微生物的方法，其中可以将外源DNA引入宿主细胞并自主复制。
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公开(公告)号：US4710471A
公开(公告)日：1987-12-01
申请号：US668674
申请日：1984-11-05
申请人： Ryoichi Katsumata , Akio Ozaki , Tetsuo Oka , Akira Furuya
发明人： Ryoichi Katsumata , Akio Ozaki , Tetsuo Oka , Akira Furuya
IPC分类号： C12N15/09 , C07H21/04 , C12N1/20 , C12N1/21 , C12N15/00 , C12N15/77 , C12P19/34 , C12R1/07 , C12R1/13 , C12R1/15 , C12R1/185 , C12R1/44 , C12N1/00
CPC分类号： C12N15/77
摘要： Disclosed are recombinant plasmid vectors constructed of a plasmid autonomously replicable in cells of microorganisms belonging to the genus Corynebacterium or Brevibacterium and a DNA fragment containing a gene expressible by said microorganisms. The recombinant vector plasmids are autonomously replicable in glutamic acid--producing microorganisms and are useful to clone desired DNA fragments in such microorganisms.
摘要翻译： 公开了由质粒可自主复制的质粒构建的重组质粒载体，其属于棒杆菌属或短杆菌属的微生物的细胞，以及含有可被所述微生物表达的基因的DNA片段。 重组载体质粒可以在产生谷氨酸的微生物中自主复制，可用于克隆这些微生物中所需的DNA片段。
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公开(公告)号：US4617267A
公开(公告)日：1986-10-14
申请号：US573591
申请日：1984-01-25
申请人： Ryoichi Katsumata , Akira Furuya
发明人： Ryoichi Katsumata , Akira Furuya
IPC分类号： C12N15/09 , C07H21/00 , C07H21/04 , C12N1/20 , C12N15/00 , C12N15/77 , C12R1/15 , C12P19/34 , C12N1/00
CPC分类号： C12N15/77
摘要： Disclosed is a novel vector, plasmid pCG1, and a process of producing plasmid pCG1 from Corynebacterium glutamicum.
摘要翻译： 公开了一种新型载体，质粒pCG1，以及从谷氨酸棒杆菌（Corynebacterium glutamicum）生产质粒pCG1的方法。
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公开(公告)号：US4500640A
公开(公告)日：1985-02-19
申请号：US368035
申请日：1982-04-13
申请人： Ryoichi Katsumata , Tetsuo Oka , Akira Furuya
发明人： Ryoichi Katsumata , Tetsuo Oka , Akira Furuya
CPC分类号： C12R1/13 , C12N15/77 , C12R1/15 , Y10S435/84 , Y10S435/843
摘要： Disclosed are novel plasmids which can replicate autonomously in microorganisms belonging to the genus Corynebacterium or Brevibacterium and which carry a gene for resistance to streptomycin and/or spectinomycin. The plasmids are useful as a cloning vector.
摘要翻译： 公开了可以在属于棒杆菌属或短杆菌属的微生物中自主复制并且携带对链霉素和/或壮观霉素具有抗性的基因的新型质粒。 质粒可用作克隆载体。
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公开(公告)号：US4489160A
公开(公告)日：1984-12-18
申请号：US410887
申请日：1982-08-24
申请人： Ryoichi Katsumata , Tetsuo Oka , Akira Furuya
发明人： Ryoichi Katsumata , Tetsuo Oka , Akira Furuya
CPC分类号： C12N15/77
摘要： Disclosed is a novel vector, plasmid pCG2, and a process of producing plasmid pCG2 from Corynebacterium glutamicum.
摘要翻译： 公开了一种新型载体，质粒pCG2，以及从谷氨酸棒杆菌（Corynebacterium glutamicum）产生质粒pCG2的方法。
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公开(公告)号：US5236831A
公开(公告)日：1993-08-17
申请号：US474648
申请日：1990-02-02
申请人： Ryoichi Katsumata , Akio Ozaki , Toru Mizukami , Motoko Kageyama , Morimasa Yagisawa , Tamio Mizukami , Seiga Itoh , Tetsuo Oka , Akira Furuya
发明人： Ryoichi Katsumata , Akio Ozaki , Toru Mizukami , Motoko Kageyama , Morimasa Yagisawa , Tamio Mizukami , Seiga Itoh , Tetsuo Oka , Akira Furuya
摘要： Disclosed is a process for expressing a gene and producing a metabolic product formed by the gene by culturing a transformant microorganism carrying a recombinant DNA constructed of a DNA fragment having at least one gene to be expressed and a vector DNA, at least one of which is foreign to the host microorganism.
摘要翻译： 公开了通过培养携带由具有至少一种待表达基因的DNA片段和载体DNA构建的重组DNA的转化体微生物来表达基因并产生由该基因形成的代谢产物的方法，其中至少一种是 外来宿主微生物。
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公开(公告)号：US4681847A
公开(公告)日：1987-07-21
申请号：US695574
申请日：1985-01-28
申请人： Ryoichi Katsumata , Tetsuo Oka , Akira Furuya
发明人： Ryoichi Katsumata , Tetsuo Oka , Akira Furuya
IPC分类号： C12N15/77 , C12N15/00 , C12N1/00 , C12N1/06 , C12N1/20 , C12N9/36 , C12P19/34 , C12P21/00 , C12R1/13 , C12R1/15
CPC分类号： C12R1/13 , C12N15/77 , C12R1/15 , Y10S435/84 , Y10S435/843
摘要： A novel lysozyme-sensitive microorganism belonging to the genus Corynebacterium or Brevibacterium and having a sensitivity to lysozyme at a concentration of less than 25 .mu.g/ml is provided from selected mutants. This novel microorganism is especially suitable for use in recombinant DNA technology.
摘要翻译： 从选择的突变体提供属于棒杆菌属或短杆菌属的新型溶菌酶敏感性微生物，其浓度低于25μg/ ml对溶菌酶具有敏感性。 该新型微生物特别适用于重组DNA技术。
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8.发明授权CHOLESTEROL DEHYDROGENASE, COPROSTAN-3-ONE DEHYDROGENASE AND 4-CHOLESTEN-3-ONE DEHYDROGENASE, COMPOSITIONS CONTAINING THE DEHYDROGENASES, AND METHOD FOR REDUCING AMOUNT OF CHOLESTEROL USING THE COMPOSITIONS 失效胆固醇脱氢酶，COPROSTAN-3-ONE脱氢酶和4-烯丙基-3-酮脱氢酶，含有脱氢酶的组合物，以及使用组合物降低胆固醇含量的方法公开(公告)号：US06485931B2
公开(公告)日：2002-11-26
申请号：US09750692
申请日：2001-01-02
申请人： Chiaki Saitoh , Hideyo Kumazawa , Kazuo Aisaka , Toru Mizukami , Katsuhiko Ando , Keiko Ochiai , Ryoichi Katsumata
发明人： Chiaki Saitoh , Hideyo Kumazawa , Kazuo Aisaka , Toru Mizukami , Katsuhiko Ando , Keiko Ochiai , Ryoichi Katsumata
IPC分类号： C12P104
CPC分类号： C12Y101/99001 , A23K20/189 , A23L5/25 , A23L13/48 , A23L15/25 , A23L17/65 , C12N9/0006 , C12P33/00
摘要： According to the present invention, a process for producing a practical cholesterol-reduced substance by converting cholesterol contained in foods and feeds to coprostanol having very low intestinal tract absorbability by utilizing enzymatic action is provided. Cholesterol in a cholesterol-containing substance such as meat, egg, milk, seafood and cooked processed foods containing the same, or feeds for animals, poultry and pisciculture, and the like, can be treated with three kinds of enzymes which are a cholesterol dehydrogenase having an optimum pH in a neutral pH range and 4-cholesten-3-one dehydrogenase and coprostan-3-one dehydrogenase each having an optimum pH in a weak acidic range, or microbial cells containing the enzymes, for converting the cholesterol to coprostanol to reduce the amount of the cholesterol.
摘要翻译： 根据本发明，提供了通过利用酶作用将食品和饲料中所含的胆固醇转化为具有非常低肠吸收能力的丙泊酚的生产实用性降胆固醇物质的方法。含胆固醇的物质如肉， 鸡蛋，牛奶，海产品，含有该食品的熟化加工食品，动物，禽类和养殖用饲料等都可以用三种酶在中性pH范围内具有最佳pH值的胆固醇脱氢酶 - 胆固醇-3-酮脱氢酶和拮抗佐-3-酮脱氢酶，其各自具有弱酸性范围内的最佳pH，或含有酶的微生物细胞，用于将胆固醇转化为coprostanol以降低胆固醇的量。
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公开(公告)号：US5559016A
公开(公告)日：1996-09-24
申请号：US170936
申请日：1993-12-21
IPC分类号： C12N1/21 , C12N9/06 , C12N15/09 , C12N15/53 , C12P13/06 , C12R1/06 , C12R1/13 , C12R1/15 , C12N9/02
CPC分类号： C12N9/0016 , C12P13/06
摘要： A process for producing alanine which comprises culturing in a medium a microorganism belonging to the genus Escherichia, Corynebacterium or Brevibacterium which has an L-alanine dehydrogenase activity and is capable of producing alanine, allowing the microorganism to produce and accumulate alanine in the culture medium, and recovering alanine from the culture medium.
摘要翻译： 一种制备丙氨酸的方法，其包括在培养基中培养属于具有L-丙氨酸脱氢酶活性并具有L-丙氨酸脱氢酶活性并具有L-丙氨酸脱氢酶活性的埃希氏杆菌属，棒状杆菌属或短杆菌属的微生物，使微生物在培养基中产生和积累丙氨酸， 并从培养基中回收丙氨酸。
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公开(公告)号：US5439822A
公开(公告)日：1995-08-08
申请号：US938333
申请日：1992-08-28
申请人： Ryoichi Katsumata , Yutaka Takano
发明人： Ryoichi Katsumata , Yutaka Takano
IPC分类号： C12N1/21 , C12N9/10 , C12N9/38 , C12N9/88 , C12N15/09 , C12N15/60 , C12N15/77 , C12P21/02 , C12R1/01 , C12R1/13 , C12R1/15 , C12N15/11
CPC分类号： C12N9/1033 , C12N15/77 , C12N9/88 , C12Y401/03001
摘要： The present invention provides a gene expression regulatory DNA and a process for preparing a protein using the same.A DNA derived from the isocitrate lyase (ICL) gene of a coryneform bacterium regulates expression of a structural gene encoding a protein when incorporated into a vector DNA together with said structural gene and introduced into a host coryneform bacterium, and a useful protein can be efficiently produced using the DNA.
摘要翻译： 本发明提供了一种基因表达调控DNA及其制备方法。 衍生自棒状杆菌型细菌的异柠檬酸裂解酶（ICL）基因的DNA在与所述结构基因一起掺入载体DNA中时调节编码蛋白质的结构基因的表达，并引入宿主棒状细菌，有效的蛋白质可以有效地 使用DNA产生。
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