公开(公告)号：US06485931B2

公开(公告)日：2002-11-26

申请号：US09750692

申请日：2001-01-02

申请人： Chiaki Saitoh ,  Hideyo Kumazawa ,  Kazuo Aisaka ,  Toru Mizukami ,  Katsuhiko Ando ,  Keiko Ochiai ,  Ryoichi Katsumata

发明人： Chiaki Saitoh ,  Hideyo Kumazawa ,  Kazuo Aisaka ,  Toru Mizukami ,  Katsuhiko Ando ,  Keiko Ochiai ,  Ryoichi Katsumata

IPC分类号： C12P104

CPC分类号： C12Y101/99001 ,  A23K20/189 ,  A23L5/25 ,  A23L13/48 ,  A23L15/25 ,  A23L17/65 ,  C12N9/0006 ,  C12P33/00

摘要： According to the present invention, a process for producing a practical cholesterol-reduced substance by converting cholesterol contained in foods and feeds to coprostanol having very low intestinal tract absorbability by utilizing enzymatic action is provided. Cholesterol in a cholesterol-containing substance such as meat, egg, milk, seafood and cooked processed foods containing the same, or feeds for animals, poultry and pisciculture, and the like, can be treated with three kinds of enzymes which are a cholesterol dehydrogenase having an optimum pH in a neutral pH range and 4-cholesten-3-one dehydrogenase and coprostan-3-one dehydrogenase each having an optimum pH in a weak acidic range, or microbial cells containing the enzymes, for converting the cholesterol to coprostanol to reduce the amount of the cholesterol.

摘要翻译： 根据本发明，提供了通过利用酶作用将食品和饲料中所含的胆固醇转化为具有非常低肠吸收能力的丙泊酚的生产实用性降胆固醇物质的方法。含胆固醇的物质如肉， 鸡蛋，牛奶，海产品，含有该食品的熟化加工食品，动物，禽类和养殖用饲料等都可以用三种酶在中性pH范围内具有最佳pH值的胆固醇脱氢酶 - 胆固醇-3-酮脱氢酶和拮抗佐-3-酮脱氢酶，其各自具有弱酸性范围内的最佳pH，或含有酶的微生物细胞，用于将胆固醇转化为coprostanol以降低胆固醇的量。

公开(公告)号：US06312919B1

公开(公告)日：2001-11-06

申请号：US09101327

申请日：1998-07-07

申请人： Chiaki Saitoh ,  Hideyo Kumazawa ,  Kazuo Aisaka ,  Toru Mizukami ,  Katsuhiko Ando ,  Keiko Ochiai ,  Ryoichi Katsumata ,  Kozo Ouchi

发明人： Chiaki Saitoh ,  Hideyo Kumazawa ,  Kazuo Aisaka ,  Toru Mizukami ,  Katsuhiko Ando ,  Keiko Ochiai ,  Ryoichi Katsumata ,  Kozo Ouchi

IPC分类号： C12P3300

CPC分类号： C12Y101/99001 ,  A23K20/189 ,  A23L5/25 ,  A23L13/48 ,  A23L15/25 ,  A23L17/65 ,  C12N9/0006 ,  C12P33/00

摘要： An enzymatic process is presented for reducing the cholesterol level of foods and feeds by converting the cholesterol therein to coprostanol which has a very low intestinal tract absorbability. Cholesterol in meat, egg, milk, seafood and cooked processed foods containing the same, or feeds for animals, poultry, and pisiculture can be reduced by the sequential action of three enzymes isolated from a eubacterium: cholesterol dehydrogenase, 4-cholesten-3-one dehydrogenase and coprostan-3-one dehydrogenase.

摘要翻译： 提出了一种酶法，用于通过将其中的胆固醇转化成具有非常低的肠道吸收性的coprostanol来降低食物和饲料的胆固醇水平。 肉，蛋，牛奶，海鲜和含有此类食物的熟化加工食品中的胆固醇或动物，家禽和养殖用饲料可以通过从真菌分离的三种酶的顺序作用来降低：胆固醇脱氢酶，4-胆甾醇-3- 一种脱氢酶和副雄甾-3-酮脱氢酶。

公开(公告)号：US5607848A

公开(公告)日：1997-03-04

申请号：US395707

申请日：1995-02-28

申请人： Ryoichi Katsumata ,  Shinichi Hashimoto ,  Keiko Ochiai

发明人： Ryoichi Katsumata ,  Shinichi Hashimoto ,  Keiko Ochiai

IPC分类号： C12P41/00 ,  C12N1/20 ,  C12P7/50 ,  C12R1/01 ,  C12R1/07 ,  C12R1/38 ,  C12R1/40 ,  C12R1/41

CPC分类号： C12R1/41 ,  C12P7/50 ,  Y10S435/822 ,  Y10S435/874 ,  Y10S435/877

摘要： The present invention provides a process for producing an optically active 4-hydroxy-2-ketoglutaric acid, by mixing glyoxylic acid, pyruvic acid and a microorganism to form the optically active 4-hydroxy-2-ketoglutaric acid in an aqueous medium.

摘要翻译： 本发明提供了通过在水性介质中混合乙醛酸，丙酮酸和微生物以形成光学活性的4-羟基-2-酮戊二酸来制备光学活性的4-羟基-2-酮戊二酸的方法。

公开(公告)号：US5153120A

公开(公告)日：1992-10-06

申请号：US652151

申请日：1991-02-07

申请人： Ryoichi Katsumata ,  Toru Mizukami ,  Shigenori Ohta ,  Moriyuki Sato ,  Kazuo Yamaguchi

发明人： Ryoichi Katsumata ,  Toru Mizukami ,  Shigenori Ohta ,  Moriyuki Sato ,  Kazuo Yamaguchi

IPC分类号： C12N15/09 ,  C12N9/10 ,  C12N15/54 ,  C12R1/125

CPC分类号： C12N9/104 ,  Y10S435/832

摘要： The present invention provides a recombinant DNA comprised of a vector DNA and a DNA fragment containing a gene coding for .gamma.-glutamyl transpeptidase derived from Bacillus subtilis. The invention also provides a process for producing .gamma.-glutamyl transpeptidase, which comprises culturing in a culture medium a microorganism belonging to the genus Bacillus which is carrying recombinant DNA comprised of a vector DNA and DNA fragment which contains a gene coding for .gamma.-glutamyl transpeptidase derived from the genus Bacillus, accumulating .gamma.-glutamyl transpeptidase in the culture, and recovering .gamma.-glutamyl transpeptidase therefrom.

公开(公告)号：US5236831A

公开(公告)日：1993-08-17

申请号：US474648

申请日：1990-02-02

申请人： Ryoichi Katsumata ,  Akio Ozaki ,  Toru Mizukami ,  Motoko Kageyama ,  Morimasa Yagisawa ,  Tamio Mizukami ,  Seiga Itoh ,  Tetsuo Oka ,  Akira Furuya

发明人： Ryoichi Katsumata ,  Akio Ozaki ,  Toru Mizukami ,  Motoko Kageyama ,  Morimasa Yagisawa ,  Tamio Mizukami ,  Seiga Itoh ,  Tetsuo Oka ,  Akira Furuya

IPC分类号： C12N9/88 ,  C12N15/52 ,  C12N15/77 ,  C12P13/08 ,  C12P13/14

CPC分类号： C12N15/52 ,  C12N15/77 ,  C12N9/88 ,  C12P13/08 ,  C12P13/14

摘要： Disclosed is a process for expressing a gene and producing a metabolic product formed by the gene by culturing a transformant microorganism carrying a recombinant DNA constructed of a DNA fragment having at least one gene to be expressed and a vector DNA, at least one of which is foreign to the host microorganism.

摘要翻译： 公开了通过培养携带由具有至少一种待表达基因的DNA片段和载体DNA构建的重组DNA的转化体微生物来表达基因并产生由该基因形成的代谢产物的方法，其中至少一种是 外来宿主微生物。

公开(公告)号：US4927758A

公开(公告)日：1990-05-22

申请号：US89922

申请日：1987-08-25

申请人： Toru Mizukami ,  Ryoichi Katsumata ,  Tetsuo Oka

发明人： Toru Mizukami ,  Ryoichi Katsumata ,  Tetsuo Oka

IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N15/52 ,  C12P13/04 ,  C12P13/06 ,  C12P13/10 ,  C12P13/22 ,  C12P13/24 ,  C12R1/13 ,  C12R1/15

CPC分类号： C12P13/04 ,  C12N15/52 ,  C12P13/06 ,  C12P13/10 ,  C12P13/22 ,  Y10S435/84 ,  Y10S435/843

摘要： Disclosed is a process for producing histidine by transforming a host microorganism belonging to the genus Corynebacterium or Brevibacterium with a recombinant DNA of a DNA fragment containing a gene involved in the biosynthesis of histidine and a vector DNA, culturing the transformant in a nutrient medium, accumulating histidine in the culture medium and recovering histidine therefrom.

摘要翻译： 公开了通过用包含组氨酸生物合成的基因的DNA片段的重组DNA和载体DNA转化属于棒状杆菌属或短杆菌属的宿主微生物来培养组氨酸的方法，在营养培养基中培养转化体， 组氨酸并从其中回收组氨酸。

公开(公告)号：US4954441A

公开(公告)日：1990-09-04

申请号：US356614

申请日：1989-05-25

申请人： Ryoichi Katsumata ,  Toru Mizukami ,  Tetsuo Oka

发明人： Ryoichi Katsumata ,  Toru Mizukami ,  Tetsuo Oka

IPC分类号： C12N15/09 ,  C12N1/20 ,  C12N1/21 ,  C12N15/00 ,  C12N15/31 ,  C12N15/52 ,  C12N15/77 ,  C12P13/08 ,  C12R1/13 ,  C12R1/15

CPC分类号： C12N15/52 ,  C12N15/77 ,  C12P13/08 ,  Y10S435/84 ,  Y10S435/843

摘要： A process for producing L-lysine is disclosed in which a recombinant vector, a DNA fragment of which contains a gene involved in the synthesis of dihydropicolinic acid synthetase, is used to transform a microorganism of the genus Corynebacterium or Brevibacterium. The transformant is then cultured in a medium which supports the accumulation of L-lysine. Subsequently the L-lysine is recovered from the culture broth.

摘要翻译： 公开了一种生产L-赖氨酸的方法，其中重组载体，其DNA片段含有涉及二氢吡啶甲酸合成酶合成的基因，用于转化棒状杆菌属或短杆菌属的微生物。 然后将转化体在支持L-赖氨酸的积累的培养基中培养。 随后从培养液中回收L-赖氨酸。

公开(公告)号：US5559016A

公开(公告)日：1996-09-24

申请号：US170936

申请日：1993-12-21

申请人： Ryoichi Katsumata ,  Shin'ichi Hashimoto

发明人： Ryoichi Katsumata ,  Shin'ichi Hashimoto

IPC分类号： C12N1/21 ,  C12N9/06 ,  C12N15/09 ,  C12N15/53 ,  C12P13/06 ,  C12R1/06 ,  C12R1/13 ,  C12R1/15 ,  C12N9/02

CPC分类号： C12N9/0016 ,  C12P13/06

摘要： A process for producing alanine which comprises culturing in a medium a microorganism belonging to the genus Escherichia, Corynebacterium or Brevibacterium which has an L-alanine dehydrogenase activity and is capable of producing alanine, allowing the microorganism to produce and accumulate alanine in the culture medium, and recovering alanine from the culture medium.

摘要翻译： 一种制备丙氨酸的方法，其包括在培养基中培养属于具有L-丙氨酸脱氢酶活性并具有L-丙氨酸脱氢酶活性并具有L-丙氨酸脱氢酶活性的埃希氏杆菌属，棒状杆菌属或短杆菌属的微生物，使微生物在培养基中产生和积累丙氨酸， 并从培养基中回收丙氨酸。

公开(公告)号：US5439822A

公开(公告)日：1995-08-08

申请号：US938333

申请日：1992-08-28

申请人： Ryoichi Katsumata ,  Yutaka Takano

发明人： Ryoichi Katsumata ,  Yutaka Takano

IPC分类号： C12N1/21 ,  C12N9/10 ,  C12N9/38 ,  C12N9/88 ,  C12N15/09 ,  C12N15/60 ,  C12N15/77 ,  C12P21/02 ,  C12R1/01 ,  C12R1/13 ,  C12R1/15 ,  C12N15/11

CPC分类号： C12N9/1033 ,  C12N15/77 ,  C12N9/88 ,  C12Y401/03001

摘要： The present invention provides a gene expression regulatory DNA and a process for preparing a protein using the same.A DNA derived from the isocitrate lyase (ICL) gene of a coryneform bacterium regulates expression of a structural gene encoding a protein when incorporated into a vector DNA together with said structural gene and introduced into a host coryneform bacterium, and a useful protein can be efficiently produced using the DNA.

摘要翻译： 本发明提供了一种基因表达调控DNA及其制备方法。 衍生自棒状杆菌型细菌的异柠檬酸裂解酶（ICL）基因的DNA在与所述结构基因一起掺入载体DNA中时调节编码蛋白质的结构基因的表达，并引入宿主棒状细菌，有效的蛋白质可以有效地 使用DNA产生。

公开(公告)号：US4775623A

公开(公告)日：1988-10-04

申请号：US646512

申请日：1984-08-31

申请人： Ryoichi Katsumata ,  Haruhiko Yokoi ,  Tetsuo Oka

发明人： Ryoichi Katsumata ,  Haruhiko Yokoi ,  Tetsuo Oka

IPC分类号： C12N15/09 ,  C12N15/00 ,  C12P13/04 ,  C12P13/06 ,  C12P13/10 ,  C12P13/22 ,  C12P13/24 ,  C12R1/13 ,  C12R1/15 ,  C12R1/19 ,  C12N1/20

CPC分类号： C12P13/04 ,  C12P13/06 ,  C12P13/10 ,  C12P13/22 ,  C12P13/24 ,  Y10S435/84 ,  Y10S435/843

摘要： Disclosed is a process for producing L-arginine by transforming a host microorganism belonging to the genus Corynebacterium or Brevibacterium with a recombinant DNA of a DNA fragment containing a gene involved in the biosynthesis of L-arginine and a vector DNA, culturing the transformant in a nutrient medium, accumulating L-arginine in the culture medium and recovering L-arginine therefrom.

摘要翻译： 公开了通过使用含有参与L-精氨酸生物合成的基因的DNA片段和载体DNA的重组DNA转化属于棒状杆菌属或短杆菌属的宿主微生物来生产L-精氨酸的方法，将转化体培养于 营养培养基，在培养基中积聚L-精氨酸，从中回收L-精氨酸。