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    Scanning Ion Conductance Microscopy
    4.
    发明申请
    Scanning Ion Conductance Microscopy 审中-公开
    扫描离子电导显微镜

    公开(公告)号：US20110131690A1

    公开(公告)日：2011-06-02

    申请号：US12864302

    申请日：2009-02-02

    申请人： Pavel Novak ,  Chao Li ,  Andrew Shevchuk ,  Victor Ostanin ,  David Klenerman ,  Yuri Korchev ,  Gregory Frolenkov ,  Richard Clarke

    发明人： Pavel Novak ,  Chao Li ,  Andrew Shevchuk ,  Victor Ostanin ,  David Klenerman ,  Yuri Korchev ,  Gregory Frolenkov ,  Richard Clarke

    IPC分类号： G01Q60/44

    CPC分类号： G01Q60/44 ,  B82Y35/00 ,  G01Q10/00 ,  G01Q10/02 ,  G01Q10/065

    摘要： The subject invention concerns methods for interrogating a surface using scanning ion conductance microscopy (SICM). In one embodiment, a method of the invention comprises the steps of: a) repeatedly bringing a SICM probe into proximity with the surface at discrete, spaced locations in a region of the surface and measuring surface height at each location; b) estimating surface roughness or other characteristic for the region based upon the surface height measurements; and c) repeatedly bringing the probe into proximity with the surface at discrete, spaced locations in the region, the number and location of which is based upon the estimated surface roughness or other characteristic in the region, and obtaining an image of the region with a resolution adapted to the surface roughness or other characteristic.

    摘要翻译： 本发明涉及使用扫描离子电导显微镜（SICM）询问表面的方法。 在一个实施例中，本发明的方法包括以下步骤：a）在表面的一个区域中的不连续，间隔的位置处重复使SICM探针与表面接近，并测量每个位置处的表面高度; b）基于表面高度测量来估计区域的表面粗糙度或其他特性; 以及c）在所述区域中的离散的间隔位置处重复地使所述探针靠近所述表面，所述位置的数量和位置基于所估计的表面粗糙度或所述区域中的其它特征，并且获得所述区域的图像 分辨率适应于表面粗糙度或其他特性。

    Measurement and use of molecular interactions
    5.
    发明授权
    Measurement and use of molecular interactions 有权
    测量和使用分子相互作用

    公开(公告)号：US07763475B2

    公开(公告)日：2010-07-27

    申请号：US11706654

    申请日：2007-02-13

    申请人： David Klenerman ,  Victor Petrovich Ostanin ,  Fedor Nikolaievich Dultsev ,  Matthew Cooper

    发明人： David Klenerman ,  Victor Petrovich Ostanin ,  Fedor Nikolaievich Dultsev ,  Matthew Cooper

    IPC分类号： G01N33/551 ,  G01N33/552

    CPC分类号： C07K1/14

    摘要： The present invention is based on the realization that the bonds between a target molecule, or a target molecule attached to a particle, and a surface, can be ruptured by mechanically oscillating the surface at increasing amplitude, leading to detachment of the target molecule or particle from the surface. The required acceleration, and hence force, will depend on a variety of factors, including the mass of the molecule or particle, the nature of the bond to the surface and the geometric shape or size of the target molecule or particle. The present invention may therefore be used to separate or to size different target molecules, or to detect their presence.

    摘要翻译： 本发明基于通过以增加的幅度机械振荡表面而导致目标分子或附着于颗粒的靶分子与表面之间的键可能破裂，导致靶分子或颗粒的分离 从表面。 所需的加速度，因而力将取决于多种因素，包括分子或颗粒的质量，与表面的键的性质以及靶分子或颗粒的几何形状或尺寸。 因此，本发明可以用于分离或调整不同的靶分子或检测其存在。

    Measurement and use of molecular interactions
    7.
    发明申请
    Measurement and use of molecular interactions 有权
    测量和使用分子相互作用

    公开(公告)号：US20070218534A1

    公开(公告)日：2007-09-20

    申请号：US11706654

    申请日：2007-02-13

    申请人： David Klenerman ,  Victor Ostanin ,  Fedor Dultsev ,  Matthew Cooper

    发明人： David Klenerman ,  Victor Ostanin ,  Fedor Dultsev ,  Matthew Cooper

    IPC分类号： C12N13/00 ,  C12M1/42

    CPC分类号： C07K1/14

    摘要： The present invention is based on the realization that the bonds between a target molecule, or a target molecule attached to a particle, and a surface, can be ruptured by mechanically oscillating the surface at increasing amplitude, leading to detachment of the target molecule or particle from the surface. The required acceleration, and hence force, will depend on a variety of factors, including the mass of the molecule or particle, the nature of the bond to the surface and the geometric shape or size of the target molecule or particle. The present invention may therefore be used to separate or to size different target molecules, or to detect their presence.

    摘要翻译： 本发明基于通过以增加的幅度机械振荡表面而导致目标分子或附着于颗粒的靶分子与表面之间的键可能破裂，导致靶分子或颗粒的分离 从表面。 所需的加速度，因而力将取决于多种因素，包括分子或颗粒的质量，与表面的键的性质以及靶分子或颗粒的几何形状或尺寸。 因此，本发明可以用于分离或调整不同的靶分子或检测其存在。

    Measurement and use of molecular interactions
    8.
    发明授权
    Measurement and use of molecular interactions 有权
    测量和使用分子相互作用

    公开(公告)号：US07195909B2

    公开(公告)日：2007-03-27

    申请号：US10350892

    申请日：2003-01-24

    申请人： David Klenerman ,  Victor Petrovich Ostanin ,  Fedor Nikolaievich Dultsev

    发明人： David Klenerman ,  Victor Petrovich Ostanin ,  Fedor Nikolaievich Dultsev

    IPC分类号： C12M3/00

    CPC分类号： G01N33/54373 ,  G01N33/5438 ,  Y10S435/808 ,  Y10S436/805

    摘要： The subject invention pertains to a method for determining the affinity between binding partners, or a property of one of the binding partners dependent on the affinity, comprising the steps of: (i) contacting the binding partners, one of which is immobilised on a surface; (ii) oscillating the surface at increasing amplitude; and (iii) detecting a dissociation event. An analogous method can be used to separate a target analyte from a composition. The subject invention also pertains to an apparatus for determining the affinity between binding partners, and comprises: a surface (10) having one binding partner (16) immobilised thereon; means for oscillating the surface at increasing amplitude; and means (14, 15) for detecting a dissociation event.

    摘要翻译： 本发明涉及用于确定结合配偶体之间的亲和力的方法，或者是依赖于亲和力的一种结合配偶体的性质，其包括步骤

    Measurement and use of molecular interactions
    10.
    发明授权
    Measurement and use of molecular interactions 有权
    测量和使用分子相互作用

    公开(公告)号：US06589727B1

    公开(公告)日：2003-07-08

    申请号：US09700485

    申请日：2000-11-15

    申请人： David Klenerman ,  Victor Petrovich Ostanin ,  Fedor Nikolaievich Dultsev

    发明人： David Klenerman ,  Victor Petrovich Ostanin ,  Fedor Nikolaievich Dultsev

    IPC分类号： C12Q300

    CPC分类号： G01N33/54373 ,  G01N33/5438 ,  Y10S435/808 ,  Y10S436/805

    摘要： A method for determining the affinity between binding partners, or a property of one of the binding partners dependent on the affinity, comprising the steps of: (i) contacting the binding partners, one of which is immobilised on a surface; (ii) oscillating the surface at increasing amplitude; and (iii) detecting a dissociation event. An analogous method can be used to separate a target analyte from a composition. The subject invention also pertains to an apparatus for determining the affinity between binding partners, and comprises: a surface (10) having one binding partner (16) immobilised thereon; means for oscillating the surface at increasing amplitude; and a device (14, 15) for detecting a dissociation event.

    摘要翻译： 用于确定结合配偶体之间的亲和力或依赖于亲和力的结合配偶体之一的性质的方法，包括以下步骤：（i）使结合伴侣（其中一个固定在表面上）;（ii） 表面增大振幅; 和（iii）检测解离事件。可以使用类似的方法从组合物中分离靶分析物。 本发明还涉及用于确定结合配偶体之间的亲和力的装置，并且包括：具有固定在其上的一个结合配偶体（16）的表面（10） 用于以增加的振幅振荡表面的装置; 以及用于检测解离事件的装置（14,15）。