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公开(公告)号：US20190233481A1
公开(公告)日：2019-08-01
申请号：US16312357
申请日：2017-06-23
Applicant: Spiber Inc. , KOJIMA INDUSTRIES CORPORATION
Inventor： Keisuke MORITA , Koichi KOTAKA
IPC: C07K14/435
CPC classification number: C07K14/43518 , C07K14/435 , C07K19/00 , C12N5/10 , C12N15/09
Abstract: The present invention relates to a modified fibroin including a domain sequence represented by Formula 1: [(A)n motif-REP]m, in which the domain sequence has an amino acid sequence locally containing a region with locally high hydropathy index equivalent to an amino acid sequence in which one or a plurality of amino acid residues in REP are substituted with amino acid residues with a high hydropathy index and/or one or a plurality of amino acid residues with a high hydropathy index are inserted into REP, as compared to naturally occurring fibroin. In Formula 1, (A)n motif represents an amino acid sequence consisting of 4 to 20 amino acid residues and the number of alanine residues relative to the total number of amino acid residues in the (A)n motif is 83% or more. REP represents an amino acid sequence consisting of 10 to 200 amino acid residues. m represents an integer of 10 to 300, A plurality of (A)n motifs and the like may be the same amino acid sequence or different amino acid sequences.
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公开(公告)号：US20150376247A1
公开(公告)日：2015-12-31
申请号：US14764472
申请日：2014-04-18
Applicant: SPIBER INC.
Inventor： Toshiaki OSAWA , Keisuke MORITA
CPC classification number: C07K14/43518 , A61K8/0279 , A61K8/42 , A61K8/46 , A61K8/64 , A61K9/5169 , A61K47/42 , A61K2800/412 , A61K2800/56 , A61K2800/84 , A61Q19/00 , C08J3/05 , C08J3/07 , C08J3/12 , C08J2389/00 , C08L89/00 , C09D7/65 , C09D7/68
Abstract: Polypeptide particles of the present invention are particles of a polypeptide derived from spider silk proteins, and have an average particle size of 1000 nm or less. A method for producing polypeptide particles of the present invention includes: a solution production step in which the polypeptide is dissolved in at least one solvent selected from the group consisting of DMSO, DMF, and these with an inorganic salt, so as to obtain a solution of the polypeptide; a step in which the solution produced in the solution production step is substituted with a water-soluble solvent so as to obtain an aqueous solution of the polypeptide; and a step in which the aqueous solution of the polypeptide is dried. Thereby, the present invention provides polypeptide particles suitable for application to a living body and capable of being applied to cosmetics, etc., while identifying the properties of the polypeptide particles, and a method for producing the same.
Abstract translation: 本发明的多肽粒子是来自蜘蛛丝蛋白质的多肽的粒子，平均粒径为1000nm以下。 本发明的多肽粒子的制造方法包括：溶液制备步骤，其中将多肽溶解于至少一种选自DMSO，DMF的溶剂中，并用无机盐溶解，得到溶液 的多肽; 在溶液制备工序中产生的溶液被水溶性溶剂代替以获得多肽的水溶液的步骤; 以及将多肽的水溶液干燥的步骤。 因此，本发明提供适用于生物体并能够应用于化妆品等的多肽颗粒，同时鉴定多肽颗粒的性质及其制备方法。
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Patent Agency Ranking
公开(公告)号：US20150374833A1
公开(公告)日：2015-12-31
申请号：US14764455
申请日：2014-04-18
Applicant: SPIBER INC.
Inventor： Toshiaki OSAWA , Keisuke MORITA
CPC classification number: A61K47/42 , A61K9/0087 , A61K38/1767 , A61L26/008 , A61L27/227 , A61L27/3604 , A61L27/3687 , A61L27/52 , A61L27/60 , A61L31/145 , A61L2430/06 , C07K14/43518
Abstract: A polypeptide hydrogel of the present invention is a hydrogel of a polypeptide derived from spider silk proteins, and has a moisture content of 85.0 to 99.9 mass % based on 100 mass % of the polypeptide hydrogel. A method for producing the hydrogel includes: a solution production step in which a polypeptide derived from spider silk proteins is dissolved in at least one dissolving solvent selected from the group consisting of: (A) dimethyl sulfoxide; (B) dimethyl sulfoxide with an inorganic salt; and (C) N, N-dimethylformamide with an inorganic salt, so as to obtain a solution of the polypeptide; and a substitution step in which the solution produced in the solution production step is substituted with a water-soluble solvent. Thus, the present invention provides a polypeptide hydrogel having excellent water retentivity and a polypeptide hydrogel suitable for application to a living body.
Abstract translation: 本发明的多肽水凝胶是来自蜘蛛丝蛋白质的多肽的水凝胶，相对于多肽水凝胶100质量％，水分含量为85.0〜99.9质量％。 一种水凝胶的制造方法，其特征在于，将来自蜘蛛丝蛋白质的多肽溶解于至少一种溶解溶剂中的溶液制造工序，所述溶解溶剂选自：（A）二甲基亚砜; （B）二甲基亚砜与无机盐; 和（C）N，N-二甲基甲酰胺与无机盐反应，得到多肽的溶液; 以及其中在溶液制备步骤中产生的溶液被水溶性溶剂代替的取代步骤。 因此，本发明提供了具有优异的保水性的多肽水凝胶和适用于生物体的多肽水凝胶。
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公开(公告)号：US20150344542A1
公开(公告)日：2015-12-03
申请号：US14655027
申请日：2013-12-18
Applicant: SPIBER INC.
Inventor： Toshiaki OSAWA , Keisuke MORITA
IPC: C07K14/78 , C07K14/435 , C07K1/14
CPC classification number: C07K14/78 , C07K1/14 , C07K1/145 , C07K14/43518 , C07K14/43563
Abstract: The present invention relates to a method for partially purifying a hydrophilic recombinant protein from a host expressing a hydrophilic recombinant protein. The method includes: a solution acquisition step in which a dissolving solvent is added to the host expressing a hydrophilic recombinant protein so as to dissolve the host cell and an insoluble substance is separated therefrom so as to obtain a solution. The hydrophilic recombinant protein has a hydropathy index of 0 or less, and the dissolving solvent is an aprotic polar solvent. Preferably, the solution is subjected to solvent substitution using an aqueous solvent.
Abstract translation: 本发明涉及从表达亲水重组蛋白的宿主部分纯化亲水重组蛋白的方法。 该方法包括：溶液获取步骤，其中向表达亲水重组蛋白的宿主中加入溶解溶剂以溶解宿主细胞，并从其中分离出不溶性物质以获得溶液。 亲水重组蛋白的亲水指数为0以下，溶解性溶剂为非质子极性溶剂。 优选地，使用水性溶剂对溶液进行溶剂置换。
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公开(公告)号：US20220119463A1
公开(公告)日：2022-04-21
申请号：US17421243
申请日：2020-01-09
Applicant: Spiber Inc.
Inventor： Keisuke MORITA , Yunosuke ABE , Hiroyuki NAKAMURA , Shota TOGASHI , Tetsuo ASAKURA
IPC: C07K14/435 , C12N15/63 , D01F4/02 , C12P21/02
Abstract: The present invention relates to a modified fibroin including a domain sequence represented by Formula 1: [(A)n motif-REP]m or Formula 2: [(A)n motif-REP]m-(A)n motif, and having a serine residue content rate of less than 5.5%.
[In Formula 1 and Formula 2, the (A)n motif represents an amino acid sequence consisting of 4 to 27 amino acid residues, and the number of alanine residues with respect to the total number of amino acid residues in the (A)n motif is 80% or more. REP represents an amino acid sequence consisting of 10 to 200 amino acid residues. m represents an integer of 10 to 300. The plurality of (A)n motifs may be the same amino acid sequence or different amino acid sequences. A plurality of REPs may be the same amino acid sequence or different amino acid sequences.]-
公开(公告)号：US20150329587A1
公开(公告)日：2015-11-19
申请号：US14655017
申请日：2013-12-18
Applicant: SPIBER INC.
Inventor： Toshiaki OSAWA , Keisuke MORITA
IPC: C07K1/14 , C07K14/78 , C07K14/435
CPC classification number: C07K1/145 , C07K14/43518 , C07K14/78
Abstract: The present invention relates to a method for extracting a hydrophilic recombinant protein from a host expressing a hydrophilic recombinant protein. The method includes: a lysate acquisition step in which a dissolving solvent is added to the host expressing a hydrophilic recombinant protein so as to obtain a host cell lysate; and a step in which a diluting solvent is added to the host cell lysate obtained in the lysate acquisition step, an insoluble substance is separated from the obtained dilution, and a supernatant containing the hydrophilic recombinant protein is collected. The hydrophilic recombinant protein has a hydropathy index of 0 or less. The dissolving solvent is an aprotic polar solvent. The diluting solvent is an aqueous solvent.
Abstract translation: 本发明涉及从表达亲水重组蛋白的宿主中提取亲水性重组蛋白的方法。 该方法包括：裂解物获取步骤，其中向表达亲水性重组蛋白的宿主中加入溶解溶剂以获得宿主细胞裂解物; 在稀释溶液收集步骤中得到的宿主细胞裂解物中加入稀释溶剂的步骤，从得到的稀释液中分离不溶物质，收集含有亲水性重组蛋白质的上清液。 亲水重组蛋白的亲水指数为0以下。 溶解溶剂是非质子极性溶剂。 稀释溶剂是水性溶剂。
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公开(公告)号：US20150361144A1
公开(公告)日：2015-12-17
申请号：US14764463
申请日：2014-04-18
Applicant: SPIBER INC.
Inventor： Toshiaki OSAWA , Keisuke MORITA
CPC classification number: C07K14/43518 , A61K8/0279 , A61K8/42 , A61K8/46 , A61K8/64 , A61K8/987 , A61K9/19 , A61K47/42 , A61K2800/10 , A61K2800/56 , A61K2800/84 , A61Q19/00 , B01J20/24 , C08J9/28 , C08J2201/0484 , C08J2201/0544 , C08J2205/022 , C08J2205/028 , C08J2389/00 , C08L89/00 , C11B9/00
Abstract: A polypeptide porous body of the present invention is a porous body of a polypeptide derived from spider silk proteins. The polypeptide includes a water-insoluble polypeptide. The polypeptide porous body has an apparent density of 0.1 g/cm3 or less. A method for producing the polypeptide porous body includes: a solution production step in which the polypeptide is dissolved in at least one solvent selected from DMSO, DMF, and these with an inorganic salt, so as to obtain a solution of the polypeptide; a step in which the solution produced in the solution production step is substituted with a water-soluble solvent so as to obtain a polypeptide gel; and a step in which the polypeptide gel is dried. Thereby, the present invention provides a polypeptide porous body having excellent water absorbability and a polypeptide porous body suitable for application to a living body, and a method for producing the same.
Abstract translation: 本发明的多肽多孔体是衍生自蜘蛛丝蛋白的多肽的多孔体。 多肽包括水不溶性多肽。 多孔多孔体的表观密度为0.1g / cm 3以下。 制备多孔多孔体的方法包括：溶液制备步骤，其中将多肽溶解在至少一种选自DMSO，DMF的溶剂中，并用无机盐溶解，得到多肽的溶液; 在溶液制备工序中产生的溶液用水溶性溶剂代替以获得多肽凝胶的步骤; 以及其中多肽凝胶被干燥的步骤。 因此，本发明提供了具有优异的吸水性的多孔多孔体和适用于生物体的多孔多孔体及其制造方法。
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