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    Direct selection of cells by secretion product
    3.
    发明授权
    Direct selection of cells by secretion product 失效
    通过分泌产物直接选择细胞

    公开(公告)号：US07166423B1

    公开(公告)日：2007-01-23

    申请号：US08416920

    申请日：1993-10-21

    申请人： Stefan Miltenyi ,  Andreas Radbruch ,  Rudi Manz

    发明人： Stefan Miltenyi ,  Andreas Radbruch ,  Rudi Manz

    IPC分类号： G01N33/53 ,  G01N33/68 ,  G01N33/563

    CPC分类号： G01N33/5005 ,  G01N33/554 ,  G01N33/58 ,  Y10S435/961 ,  Y10S435/972 ,  Y10S435/975 ,  Y10S436/824

    摘要： Cells can be labeled with products which they secrete and release in an efficient manner by coupling the cells at their surface to a specific binding partner for the product and allowing the product to be captured by the specific binding partner as it is secreted and released. The product-labeled cells can then be further coupled to suitable labels, if desired, and separated according to the presence, absence, or amount of product.

    摘要翻译： 细胞可以通过将其表面上的细胞与产物的特异性结合配偶体偶联而以有效的方式分泌和释放的产物进行标记，并允许产物在分泌和释放时被特异性结合配偶体捕获。 然后，如果需要，将产物标记的细胞进一步偶合到合适的标记上，并根据产品的存在，不存在或数量分离。

    Direct selection of cells by secretion product
    4.
    发明申请
    Direct selection of cells by secretion product 审中-公开
    通过分泌产物直接选择细胞

    公开(公告)号：US20060141540A1

    公开(公告)日：2006-06-29

    申请号：US11359328

    申请日：2006-02-21

    申请人： Stefan Miltenyi ,  Andreas Radbruch ,  Rudi Manz

    发明人： Stefan Miltenyi ,  Andreas Radbruch ,  Rudi Manz

    IPC分类号： G01N33/567 ,  C12N5/06

    CPC分类号： G01N33/5005 ,  G01N33/53 ,  G01N33/56966 ,  G01N33/56972 ,  G01N33/58

    摘要： Cells can be labeled with products which they secrete and release in an efficient manner by coupling the cells at their surface to a specific binding partner for the product and allowing the product to be captured by the specific binding partner as it is secreted and released. The product-labeled cells can then be further coupled to suitable labels, if desired, and separated according to the presence, absence, or amount of product.

    摘要翻译： 细胞可以通过将其表面上的细胞与产物的特异性结合配偶体偶联而以有效的方式分泌和释放的产物进行标记，并允许产物在分泌和释放时被特异性结合配偶体捕获。 然后，如果需要，将产物标记的细胞进一步偶合到合适的标记上，并根据产品的存在，不存在或数量分离。

    Isolation and characterization of allergen-binding cells for diagnosis of hypersensitivity
    5.
    发明授权
    Isolation and characterization of allergen-binding cells for diagnosis of hypersensitivity 失效
    过敏原结合细胞的分离和表征用于诊断超敏反应

    公开(公告)号：US5916818A

    公开(公告)日：1999-06-29

    申请号：US37126

    申请日：1998-03-09

    申请人： Johannes Irsch ,  Stefan Miltenyi ,  Andreas Radbruch

    发明人： Johannes Irsch ,  Stefan Miltenyi ,  Andreas Radbruch

    IPC分类号： G01N33/543 ,  G01N33/569 ,  G01N33/53 ,  G01N33/553

    CPC分类号： G01N33/56972 ,  G01N33/54326 ,  G01N2333/05

    摘要： Methods and compositions are provided for the diagnosis of allergen hypersensitivity in a patient. Rare, allergen-specific cells are enriched from a complex cell population, e.g. a patient blood sample. The percentage of blood cells that bind to a particular allergen is less than 0.01%. The allergen-specific cell population is enriched by magnetic cell sorting. In normal blood, the allergen-binding cells are primarily B-cells expressing CD19 and CD21. In blood from allergic patients, an additional population of effector cells, e.g. basophilic granulocytes is labeled by the allergen.

    摘要翻译： 提供了用于诊断患者中过敏原超敏反应的方法和组合物。 稀有的变应原特异性细胞从复杂的细胞群富集，例如 患者血液样本。 结合特定过敏原的血细胞百分比小于0.01％。 通过磁性细胞分选富集变应原特异性细胞群。 在正常血液中，过敏原结合细胞主要是表达CD19和CD21的B细胞。 在来自过敏患者的血液中，另外的效应细胞群体，例如， 嗜碱性粒细胞被变应原标记。

    METHOD FOR IN-VITRO DETECTING PATHOGENIC T HELPER CELLS AND PHARMACEUTICAL COMPOSITIONS FOR TREATING AUTOIMMUNE DISEASES
    7.
    发明申请
    METHOD FOR IN-VITRO DETECTING PATHOGENIC T HELPER CELLS AND PHARMACEUTICAL COMPOSITIONS FOR TREATING AUTOIMMUNE DISEASES 审中-公开
    用于体内检测病原性T细胞和用于治疗自身免疫性疾病的药物组合物的方法

    公开(公告)号：US20110045004A1

    公开(公告)日：2011-02-24

    申请号：US12808821

    申请日：2008-12-22

    申请人： Andreas Radbruch ,  Uwe Niesner ,  Inka Albrecht ,  Hyun-Dong Chang ,  Torsten Stamm ,  Joachim Gruen ,  Andreas Gruetzkau

    发明人： Andreas Radbruch ,  Uwe Niesner ,  Inka Albrecht ,  Hyun-Dong Chang ,  Torsten Stamm ,  Joachim Gruen ,  Andreas Gruetzkau

    IPC分类号： A61K39/395 ,  C12Q1/68 ,  G01N33/566 ,  C07H21/02 ,  A61K38/02 ,  A61P29/00

    CPC分类号： G01N33/56972 ,  G01N33/505 ,  G01N2800/24

    摘要： The invention relates to a method for in-vitro detecting pathogenic T helper cells by incubating a sample of a body fluid or tissue taken from a mammal with substances specifically interacting with at least one gene product encoded by selected genes, which are differentially expressed compared to normal T helper cells, determining specific incubation products, correlating an amount of signal or change in signal with a concentration of the gene product in the sample, and detecting cell pathogenicity by comparing the concentration with another gene product concentration in a sample of non-pathogenic and/or pathogenic cells. Another object if the invention concerns a pharmaceutical composition for prophylaxis and therapy of chronicinflammatory. The invention also relates to a method for screening substances with the property to reduce the pathogenicity of T helper cells along with the symptoms of chronic inflammatory diseases.

    摘要翻译： 本发明涉及一种用于体外检测病原性T辅助细胞的方法，该方法通过将取自哺乳动物的体液或组织的样品与与所选基因编码的至少一种基因产物特异性相互作用的物质进行体外检测，所述基因产物与 正常T辅助细胞，确定特异性孵育产物，将信号量或信号变化与样品中基因产物的浓度相关联，并通过将浓度与非致病性样品中的另一种基因产物浓度进行比较来检测细胞致病性 和/或致病细胞。 如果本发明涉及用于预防和治疗慢性炎症的药物组合物，另一个目的。 本发明还涉及一种用于筛选具有降低T辅助细胞致病性的性质以及慢性炎性疾病症状的物质的方法。

    Process for Recognizing Signatures in Complex Gene Expression Profiles
    8.
    发明申请
    Process for Recognizing Signatures in Complex Gene Expression Profiles 审中-公开
    识别复杂基因表达谱中的特征的过程

    公开(公告)号：US20080108509A1

    公开(公告)日：2008-05-08

    申请号：US11547040

    申请日：2005-04-04

    申请人： Thomas Haupl ,  Joachim Grun ,  Andreas Radbruch ,  Gerd-Rudiger Burmester ,  Christian Kaps ,  Andreas Grutzkau

    发明人： Thomas Haupl ,  Joachim Grun ,  Andreas Radbruch ,  Gerd-Rudiger Burmester ,  Christian Kaps ,  Andreas Grutzkau

    IPC分类号： C40B30/02 ,  G06G7/48 ,  C40B60/12

    CPC分类号： C12Q1/6809

    摘要： This invention relates to a process for recognizing signatures in complex gene expression profiles that comprises the steps of: a) making available a biological sample that is to be examined, b) making available at least one suitable expression profile, whereby at least one expression profile comprises one or more markers that are typical exclusively of the expression profile, c) determining the complex expression profile of the biological sample, d) determining the quantitative cellular composition of the biological sample by means of the expression profiles determined in steps b) and c). In addition, the process according to the invention can comprise the steps of e) calculating a virtual signal that is expected based on the specific composition of the expression profile, f) calculation of the difference from the actually measured complex expression profile and the virtual signal, and g) determination of the quantitative composition of the complex expression profile based on the determined differences. In addition, this invention relates to the application of the process according to the invention in the diagnosis, prognosis and/or tracking of a disease. Finally, corresponding computer systems, computer programs, computer-readable data media and laboratory robots or evaluating devices for molecular detection methods are disclosed.

    摘要翻译： 本发明涉及用于识别复合基因表达谱中的特征的方法，其包括以下步骤：a）提供待检查的生物样品，b）提供至少一种合适的表达谱，其中至少一个表达谱 包括一个或多个仅表达谱的典型的标记，c）确定生物样品的复合物表达谱，d）通过步骤b）和c中确定的表达谱确定生物样品的定量细胞组成 ）。 此外，根据本发明的过程可以包括以下步骤：e）基于表达简档的特定组合来计算期望的虚拟信号，f）与实际测量的复合表达谱和虚拟信号的差的计算 ，以及g）基于确定的差异确定复合体表达谱的定量组成。 此外，本发明涉及根据本发明的方法在疾病的诊断，预后和/或跟踪中的应用。 最后，公开了相应的计算机系统，计算机程序，计算机可读数据介质和实验室机器人或用于分子检测方法的评估装置。