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    Method and apparatus for identifying reticulocytes within a blood sample
    3.
    发明授权
    Method and apparatus for identifying reticulocytes within a blood sample 有权
    用于鉴定血液样品内的网织红细胞的方法和装置

    公开(公告)号：US08310658B2

    公开(公告)日：2012-11-13

    申请号：US13039453

    申请日：2011-03-03

    申请人： Stephen C. Wardlaw ,  Robert A. Levine ,  Darryn W. Unfricht ,  Niten V. Lalpuria ,  Jeremy R. Hill

    发明人： Stephen C. Wardlaw ,  Robert A. Levine ,  Darryn W. Unfricht ,  Niten V. Lalpuria ,  Jeremy R. Hill

    IPC分类号： G01N33/48

    CPC分类号： G06T7/0012 ,  G01N15/05 ,  G01N33/49 ,  G01N2015/055

    摘要： A method and apparatus for identifying reticulocytes within a blood sample is provided. The method includes the steps of: a) depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, and the chamber has a known or determinable height extending between the interior surfaces of panels, which height is such that at least one red blood cell, or an aggregate of red blood cells, within the sample contacts both of the interior surfaces; b) admixing a supravital dye with the sample, which dye is operable to cause reticulin to fluoresce when excited by light of one or more predetermined wavelengths; c) imaging the sample using light that includes the one or more predetermined wavelengths that cause reticulin to fluoresce; d) imaging the sample using light that is absorbed by hemoglobin to produce values of optical density on a per image unit basis; and e) identifying reticulocytes within the sample using the image of the sample created with light that causes the dyed reticulin to fluoresce, and using the per image unit optical density values.

    摘要翻译： 提供了一种用于鉴定血液样本内的网织红细胞的方法和装置。 该方法包括以下步骤：a）将样品沉积到适于静态保持样品进行分析的分析室中，并且室具有在面板的内表面之间延伸的已知或可确定的高度，其高度使得至少一个 样品内的红血细胞或红细胞聚集体接触两个内表面; b）将supravital染料与样品混合，该染料可操作以在由一种或多种预定波长的光激发时使网状蛋白发荧光; c）使用包括导致网状蛋白发荧光的一个或多个预定波长的光成像样品; d）使用被血红蛋白吸收的光进行成像，以产生基于每个图像单位的光密度值; 以及e）使用由导致染色的网状蛋白发荧光的光产生的样品的图像来识别样品内的网织红细胞，并且使用每个图像单位的光密度值。

    METHOD AND APPARATUS FOR IDENTIFYING RETICULOCYTES WITHIN A BLOOD SAMPLE
    4.
    发明申请
    METHOD AND APPARATUS FOR IDENTIFYING RETICULOCYTES WITHIN A BLOOD SAMPLE 有权
    用于在血液样品中鉴定细胞因子的方法和装置

    公开(公告)号：US20110149061A1

    公开(公告)日：2011-06-23

    申请号：US13039453

    申请日：2011-03-03

    申请人： Stephen C. Wardlaw ,  Robert A. Levine ,  Darryn W. Unfricht ,  Niten V. Lalpuria ,  Jeremy R. Hill

    发明人： Stephen C. Wardlaw ,  Robert A. Levine ,  Darryn W. Unfricht ,  Niten V. Lalpuria ,  Jeremy R. Hill

    IPC分类号： G06K9/00 ,  H04N7/18

    CPC分类号： G06T7/0012 ,  G01N15/05 ,  G01N33/49 ,  G01N2015/055

    摘要： A method and apparatus for identifying reticulocytes within a blood sample is provided. The method includes the steps of: a) depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, and the chamber has a known or determinable height extending between the interior surfaces of panels, which height is such that at least one red blood cell, or an aggregate of red blood cells, within the sample contacts both of the interior surfaces; b) admixing a supravital dye with the sample, which dye is operable to cause reticulin to fluoresce when excited by light of one or more predetermined wavelengths; c) imaging the sample using light that includes the one or more predetermined wavelengths that cause reticulin to fluoresce; d) imaging the sample using light that is absorbed by hemoglobin to produce values of optical density on a per image unit basis; and e) identifying reticulocytes within the sample using the image of the sample created with light that causes the dyed reticulin to fluoresce, and using the per image unit optical density values.

    摘要翻译： 提供了一种用于鉴定血液样本内的网织红细胞的方法和装置。 该方法包括以下步骤：a）将样品沉积到适于静态保持样品进行分析的分析室中，并且室具有在面板的内表面之间延伸的已知或可确定的高度，其高度使得至少一个 样品内的红血细胞或红细胞聚集体接触两个内表面; b）将supravital染料与样品混合，该染料可操作以在由一种或多种预定波长的光激发时使网状蛋白发荧光; c）使用包括导致网状蛋白发荧光的一个或多个预定波长的光成像样品; d）使用被血红蛋白吸收的光进行成像，以产生基于每个图像单位的光密度值; 以及e）使用由导致染色的网状蛋白发荧光的光产生的样品的图像，以及使用每个图像单位的光密度值来鉴定样品内的网织红细胞。

    METHOD AND APPARATUS FOR DETERMINING THE HEMATOCRIT OF A BLOOD SAMPLE UTILIZING THE INTRINSIC PIGMENTATION OF HEMOGLOBIN CONTAINED WITHIN THE RED BLOOD CELLS
    9.
    发明申请
    METHOD AND APPARATUS FOR DETERMINING THE HEMATOCRIT OF A BLOOD SAMPLE UTILIZING THE INTRINSIC PIGMENTATION OF HEMOGLOBIN CONTAINED WITHIN THE RED BLOOD CELLS 有权
    用于确定使用红细胞中含有的HEMOGLOBIN的内在色素的血液样品的HEMATOCRIT的方法和装置

    公开(公告)号：US20110230740A1

    公开(公告)日：2011-09-22

    申请号：US13116837

    申请日：2011-05-26

    申请人： Robert A. Levine ,  Stephen C. Wardlaw ,  Darryn W. Unfricht ,  Niten V. Lalpuria

    发明人： Robert A. Levine ,  Stephen C. Wardlaw ,  Darryn W. Unfricht ,  Niten V. Lalpuria

    IPC分类号： A61B5/1455

    CPC分类号： G01N33/80 ,  A61B5/145 ,  G01N15/1463 ,  G01N15/1475 ,  G01N33/48 ,  G01N33/49 ,  G01N33/721 ,  G01N33/86

    摘要： A method for determining the hematocrit of a blood sample is provided that includes the steps of: 1) depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber defined by the interior surfaces of first and second panels and a height extending there between, wherein both panels are transparent, and the height is such that at least some of the red blood cells within the sample contact both interior surfaces of the panels and one or more lacunae within the quiescent sample extend between the interior surfaces; 2) imaging at least a portion of the quiescent sample, which sample portion contains the red blood cells and one or more lacunae to determine an optical density of the imaged portion of the sample on a per image unit basis; 3) selecting and averaging the optical density values of the image units aligned with the red blood cells contacting the interior surfaces, and assigning an upper boundary value of 100% to the average optical density value of those image units; 4) selecting the optical density values of the image units aligned with the one or more lacunae, and assigning a lower boundary value of 0% to the optical density values of those image units; and 5) determining the hematocrit of the sample by assigning relative values to the optical density value of each image of the imaged sample portion as a function of the upper and lower boundary values, and averaging the relative values.

    摘要翻译： 提供了一种用于确定血液样本的血细胞比容的方法，其包括以下步骤：1）将样品沉积到适于静态保持样品进行分析的分析室中，由第一和第二面板的内表面限定的腔室和 高度在其间延伸，其中两个面板是透明的，并且高度使得样品中的至少一些红细胞接触面板的两个内表面，并且静止样品内的一个或多个空隙在内表面之间延伸; 2）对静止样品的至少一部分进行成像，该样品部分包含红细胞和一个或多个空隙，以基于每个图像单位确定样品的成像部分的光密度; 3）选择和平均与内表面接触的红细胞对准的图像单元的光密度值，并将上限边界值分配给这些图像单元的平均光密度值; 4）选择与一个或多个空白对齐的图像单元的光密度值，并为这些图像单元的光密度值分配0％的下边界值; 以及5）通过将所述成像样本部分的每个图像的光密度值分配相对值作为上边界值和下边界值的函数来确定样本的血细胞比容，并平均相对值。