公开(公告)号：US07074610B2

公开(公告)日：2006-07-11

申请号：US09941661

申请日：2001-08-30

申请人： Stephen N. Cozzette ,  Graham Davis ,  Jeanne A. Itak ,  Imants R. Lauks ,  Randall M. Mier ,  Sylvia Piznik ,  Nicolaas Smit ,  Susan J. Steiner ,  Paul Van Der Werf ,  Henry J. Wieck

发明人： Stephen N. Cozzette ,  Graham Davis ,  Jeanne A. Itak ,  Imants R. Lauks ,  Randall M. Mier ,  Sylvia Piznik ,  Nicolaas Smit ,  Susan J. Steiner ,  Paul Van Der Werf ,  Henry J. Wieck

IPC分类号： C12M3/00

CPC分类号： B01L3/5027 ,  B01L3/0268 ,  B01L3/502707 ,  B01L3/5088 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0819 ,  B01L2300/089 ,  C12Q1/001 ,  G01N33/5438 ,  G01N35/0099 ,  G01N35/109 ,  G01N2035/00059 ,  G01N2035/00237 ,  G01N2035/00455 ,  G01N2035/00534 ,  G01N2035/1034 ,  G01N2035/1076 ,  Y10S435/81 ,  Y10S436/809

摘要： An efficient method for the microfabrication of electronic devices which have been adapted for the analyses of biologically significant analyte species is described. The techniques of the present invention allow for close control over the dimensional features of the various components and layers established on a suitable substrate. Such control extends to those parts of the devices which incorporate the biological components which enable these devices to function as biological sensors. The materials and methods disclosed herein thus provide an effective means for the mass production of uniform wholly microfabricated biosensors. Various embodiments of the devices themselves are described herein which are especially suited for real time analyses of biological samples in a clinical setting. In particular, the present invention describes assays which can be performed using certain ligand/ligand receptor-based biosensor embodiments. The present invention also discloses a novel method for the electrochemical detection of particular analyte species of biological and physiological significance using a substrate/label signal generating pair which produces a change in the concentration of electroactive species selected from the group consisting of dioxygen and hydrogen peroxide.

摘要翻译： 描述了适用于分析生物学重要的分析物种的电子装置的微细加工的有效方法。 本发明的技术允许对在合适的衬底上建立的各种组件和层的尺寸特征进行密切的控制。 这种控制延伸到结合生物组分的装置的那些部分，使得这些装置能够用作生物传感器。 因此，本文公开的材料和方法提供了用于批量生产均匀的全微生物传感器的有效手段。 本文描述了装置本身的各种实施例，其特别适用于临床环境中的生物样品的实时分析。 特别地，本发明描述了可以使用某些基于配体/配体受体的生物传感器实施方案进行的测定。 本发明还公开了一种利用基板/标记信号产生对电化学检测特定分析物种的生物学和生理学意义的新方法，其产生选自二氧和过氧化氢的电活性物质的浓度变化。

公开(公告)号：US5200051A

公开(公告)日：1993-04-06

申请号：US432714

申请日：1989-11-07

申请人： Stephen N. Cozzette ,  Graham Davis ,  Jeanne A. Itak ,  Imants R. Lauks ,  Randall M. Mier ,  Sylvia Piznik ,  Nicolaas Smit ,  Susan J. Steiner ,  Paul Van Der Werf ,  Henry J. Wieck

发明人： Stephen N. Cozzette ,  Graham Davis ,  Jeanne A. Itak ,  Imants R. Lauks ,  Randall M. Mier ,  Sylvia Piznik ,  Nicolaas Smit ,  Susan J. Steiner ,  Paul Van Der Werf ,  Henry J. Wieck

IPC分类号： H04R1/44 ,  B01L3/00 ,  B01L3/02 ,  C12Q1/00 ,  G01N1/28 ,  G01N27/07 ,  G01N27/327 ,  G01N27/333 ,  G01N27/414 ,  G01N27/416 ,  G01N33/543 ,  G01N35/00 ,  G01N35/10 ,  G01V9/00 ,  G10K11/00 ,  H04R20060101 ,  H04R1/02 ,  H04R15/00

CPC分类号： B01L3/0268 ,  B01L3/5027 ,  C12Q1/001 ,  G01N33/5438 ,  G01N35/0099 ,  G01V9/00 ,  G10K11/006 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0877 ,  B01L3/5088 ,  G01N2035/00059 ,  G01N2035/00237 ,  G01N2035/00455 ,  G01N2035/00534 ,  G01N2035/1034 ,  G01N2035/1076 ,  G01N35/109 ,  Y10S435/817 ,  Y10S436/806

摘要： An efficient method for the microfabrication of electronic devices which have been adapted for the analyses of biologically significant analyte species is described. The techniques of the present invention allow for close control over the dimensional features of the various components and layers established on a suitable substrate. Such control extends to those parts of the devices which incorporate the biological components which enable these devices to function as biological sensors. The materials and methods disclosed herein thus provide an effective means for the mass production of uniform wholly microfabricated biosensors. Various embodiments of the devices themselves are described herein which are especially suited for real time analyses of biological samples in a clinical setting. In particular, the present invention describes assays which can be performed using certain ligand/ligand receptor-based biosensor embodiments. The present invention also discloses a novel method for the electrochemical detection of particular analyte species of biological and physiological significance using an substrate/label signal generating pair which produces a change in the concentration of electroactive species selected from the group consisting of dioxygen and hydrogen peroxide.

摘要翻译： 描述了适用于分析生物学重要的分析物种的电子装置的微细加工的有效方法。 本发明的技术允许对在合适的衬底上建立的各种组件和层的尺寸特征进行密切的控制。 这种控制延伸到结合生物组分的装置的那些部分，使得这些装置能够用作生物传感器。 因此，本文公开的材料和方法提供了用于批量生产均匀的全微生物传感器的有效手段。 本文描述了装置本身的各种实施例，其特别适用于临床环境中的生物样品的实时分析。 特别地，本发明描述了可以使用某些基于配体/配体受体的生物传感器实施方案进行的测定。 本发明还公开了一种利用基板/标记信号产生对电化学检测特定分析物种的生物学和生理意义的新方法，其产生选自二氧和过氧化氢的电活性物质浓度的变化。

公开(公告)号：US5063081A

公开(公告)日：1991-11-05

申请号：US567870

申请日：1990-08-15

申请人： Stephen N. Cozzette ,  Graham Davis ,  Jeanne Itak ,  Imants R. Lauks ,  Randall M. Mier ,  Sylvia Piznik ,  Nicolaas Smit ,  Susan Steiner ,  Paul Van Der Werf ,  Henry J. Wieck

发明人： Stephen N. Cozzette ,  Graham Davis ,  Jeanne Itak ,  Imants R. Lauks ,  Randall M. Mier ,  Sylvia Piznik ,  Nicolaas Smit ,  Susan Steiner ,  Paul Van Der Werf ,  Henry J. Wieck

IPC分类号： B01L3/00 ,  B01L3/02 ,  C12Q1/00 ,  G01N33/543 ,  G01N35/00 ,  G01N35/10

CPC分类号： B01L3/0268 ,  B01L3/502707 ,  C12Q1/001 ,  G01N33/5438 ,  G01N35/0099 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0877 ,  B01L3/5088 ,  G01N2035/00059 ,  G01N2035/00237 ,  G01N2035/00455 ,  G01N2035/00534 ,  G01N2035/1034 ,  G01N2035/1076 ,  G01N35/109

摘要： A plurality of uniform microfabricated sensing devices are produced by establishing a plurality of base sensors on a substrate wafer, forming over at least a portion of each base sensor a permselective layer, superimposing a photoformable proteinaceous photoresist layer over a substantial portion of the permselective layer, and forming a topmost layer of an immobilized ligand receptor. The ligand receptor and corresponding ligand may be immunoreactive species.

摘要翻译： 通过在衬底晶片上建立多个基底传感器来制造多个均匀的微制造感测装置，在每个基底传感器的至少一部分上形成选择性选择层，将可光成像的蛋白质光致抗蚀剂层叠加在选择性选择层的大部分上， 并形成固定的配体受体的最上层。 配体受体和相应的配体可以是免疫反应性物质。

公开(公告)号：US06306594B1

公开(公告)日：2001-10-23

申请号：US09193370

申请日：1998-11-17

申请人： Stephen N. Cozzette ,  Graham Davis ,  Imants R. Lauks ,  Randall M. Mier ,  Sylvia Piznik ,  Nicolaas Smit ,  Paul Van der Werf ,  Henry J. Wieck

发明人： Stephen N. Cozzette ,  Graham Davis ,  Imants R. Lauks ,  Randall M. Mier ,  Sylvia Piznik ,  Nicolaas Smit ,  Paul Van der Werf ,  Henry J. Wieck

IPC分类号： G01N3350

CPC分类号： B01L3/5027 ,  B01L3/0268 ,  B01L3/502707 ,  B01L3/5088 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0819 ,  B01L2300/089 ,  C12Q1/001 ,  G01N33/5438 ,  G01N35/0099 ,  G01N35/109 ,  G01N2035/00059 ,  G01N2035/00237 ,  G01N2035/00455 ,  G01N2035/00534 ,  G01N2035/1034 ,  G01N2035/1076 ,  Y10S435/81 ,  Y10S436/809

摘要： An efficient method for the microfabrication of electronic devices which have been adapted for the analyses of biologically significant analyte species is described. The techniques of the present invention allow for close control over the dimensional features of the various components and layers established on a suitable substrate. Such control extends to those parts of the devices which incorporate the biological components which enable these devices to function as biological sensors. The materials and methods disclosed herein thus provide an effective means for the mass production of uniform wholly microfabricated biosensors. Various embodiments of the devices themselves are described herein which are especially suited for real time analyses of biological samples in a clinical setting. In particular, the present invention describes assays which can be performed using certain ligand/ligand receptor-based biosensor embodiments. The present invention also discloses a novel method for the electrochemical detection of particular analyte species of biological and physiological significance using an substrate/label signal generating pair which produces a change in the concentration of electroactive species selected from the group consisting of dioxygen and hydrogen peroxide.

摘要翻译： 描述了适用于分析生物学重要的分析物种的电子装置的微细加工的有效方法。 本发明的技术允许对在合适的衬底上建立的各种组件和层的尺寸特征进行密切的控制。 这种控制延伸到结合生物组分的装置的那些部分，使得这些装置能够用作生物传感器。 因此，本文公开的材料和方法提供了用于批量生产均匀的全微生物传感器的有效手段。 本文描述了装置本身的各种实施例，其特别适用于临床环境中的生物样品的实时分析。 特别地，本发明描述了可以使用某些基于配体/配体受体的生物传感器实施方案进行的测定。 本发明还公开了一种利用基板/标记信号产生对电化学检测特定分析物种的生物学和生理意义的新方法，其产生选自二氧和过氧化氢的电活性物质浓度的变化。

公开(公告)号：US5554339A

公开(公告)日：1996-09-10

申请号：US109507

申请日：1993-08-19

申请人： Stephen N. Cozzette ,  Graham Davis ,  Imants R. Lauks ,  Randall M. Mier ,  Sylvia Piznik ,  Nicolaas Smit ,  Paul Van Der Werf ,  Henry J. Wieck

发明人： Stephen N. Cozzette ,  Graham Davis ,  Imants R. Lauks ,  Randall M. Mier ,  Sylvia Piznik ,  Nicolaas Smit ,  Paul Van Der Werf ,  Henry J. Wieck

IPC分类号： H04R1/44 ,  B01L3/00 ,  B01L3/02 ,  C12Q1/00 ,  G01N1/28 ,  G01N27/07 ,  G01N27/327 ,  G01N27/333 ,  G01N27/414 ,  G01N27/416 ,  G01N33/543 ,  G01N35/00 ,  G01N35/10 ,  G01V9/00 ,  G10K11/00 ,  H04R20060101 ,  H04R1/02 ,  H04R15/00 ,  G01N21/00 ,  C12M1/00 ,  G01N21/01 ,  G01N30/00

CPC分类号： B01L3/0268 ,  B01L3/5027 ,  C12Q1/001 ,  G01N33/5438 ,  G01N35/0099 ,  G01V9/00 ,  G10K11/006 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0877 ,  B01L3/5088 ,  G01N2035/00059 ,  G01N2035/00237 ,  G01N2035/00455 ,  G01N2035/00534 ,  G01N2035/1034 ,  G01N2035/1076 ,  G01N35/109 ,  Y10S435/817 ,  Y10S436/806

摘要： An efficient method for the microfabrication of electronic devices which have been adapted for the analyses of biologically significant analyte species is described. The techniques of the present invention allow for close control over the dimensional features of the various components and layers established on a suitable substrate. Such control extends to those parts of the devices which incorporate the biological components which enable these devices to function as biological sensors. The materials and methods disclosed herein thus provide an effective means for the mass production of uniform wholly microfabricated biosensors. Various embodiments of the devices themselves are described herein which are especially suited for real time analyses of biological samples in a clinical setting. In particular, the present invention describes assays which can be performed using certain ligand/ligand receptor-based biosensor embodiments. The present invention also discloses a novel method for the electrochemical detection of particular analyte species of biological and physiological significance using an substrate/label signal generating pair which produces a change in the concentration of electroactive species selected from the group consisting of dioxygen and hydrogen peroxide.

公开(公告)号：US5466575A

公开(公告)日：1995-11-14

申请号：US943345

申请日：1992-09-10

申请人： Stephen N. Cozzette ,  Graham Davis ,  Imants R. Lauks ,  Randall M. Mier ,  Sylvia Piznik ,  Nicolaas Smit ,  Paul Van Der Werf ,  Henry J. Wieck

发明人： Stephen N. Cozzette ,  Graham Davis ,  Imants R. Lauks ,  Randall M. Mier ,  Sylvia Piznik ,  Nicolaas Smit ,  Paul Van Der Werf ,  Henry J. Wieck

IPC分类号： H04R1/44 ,  B01L3/00 ,  B01L3/02 ,  C12Q1/00 ,  G01N1/28 ,  G01N27/07 ,  G01N27/327 ,  G01N27/333 ,  G01N27/414 ,  G01N27/416 ,  G01N33/543 ,  G01N35/00 ,  G01N35/10 ,  G01V9/00 ,  G10K11/00 ,  H04R20060101 ,  H04R1/02 ,  H04R15/00

CPC分类号： B01L3/0268 ,  B01L3/5027 ,  C12Q1/001 ,  G01N33/5438 ,  G01N35/0099 ,  G01V9/00 ,  G10K11/006 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0877 ,  B01L3/5088 ,  G01N2035/00059 ,  G01N2035/00237 ,  G01N2035/00455 ,  G01N2035/00534 ,  G01N2035/1034 ,  G01N2035/1076 ,  G01N35/109 ,  Y10S435/817 ,  Y10S436/806

摘要： An efficient method for the microfabrication of electronic devices which have been adapted for the analyses of biologically significant analyte species is described. The techniques of the present invention allow for close control over the dimensional features of the various components and layers established on a suitable substrate. Such control extends to those parts of the devices which incorporate the biological components which enable these devices to function as biological sensors. The materials and methods disclosed herein thus provide an effective means for the mass production of uniform wholly microfabricated biosensors. Various embodiments of the devices themselves are described herein which are especially suited for real time analyses of biological samples in a clinical setting. In particular, the present invention describes assays which can be performed using certain ligand/ligand receptor-based biosensor embodiments. The present invention also discloses a novel method for the electrochemical detection of particular analyte species of biological and physiological significance using an substrate/label signal generating pair which produces a change in the concentration of electroactive species selected from the group consisting of dioxygen and hydrogen peroxide.

摘要翻译： 描述了适用于分析生物学重要的分析物种的电子装置的微细加工的有效方法。 本发明的技术允许对在合适的衬底上建立的各种组件和层的尺寸特征进行密切的控制。 这种控制延伸到结合生物组分的装置的那些部分，使得这些装置能够用作生物传感器。 因此，本文公开的材料和方法提供了用于批量生产均匀的全微生物传感器的有效手段。 本文描述了装置本身的各种实施例，其特别适用于临床环境中的生物样品的实时分析。 特别地，本发明描述了可以使用某些基于配体/配体受体的生物传感器实施方案进行的测定。 本发明还公开了一种利用基板/标记信号产生对电化学检测特定分析物种的生物学和生理意义的新方法，其产生选自二氧和过氧化氢的电活性物质浓度的变化。

公开(公告)号：US5212050A

公开(公告)日：1993-05-18

申请号：US568441

申请日：1990-08-15

申请人： Randall M. Mier ,  Sylvia Piznik ,  Imants R. Lauks ,  Graham Davis

发明人： Randall M. Mier ,  Sylvia Piznik ,  Imants R. Lauks ,  Graham Davis

IPC分类号： B01L3/00 ,  B01L3/02 ,  C12Q1/00 ,  G01N33/543 ,  G01N35/00 ,  G01N35/10

CPC分类号： B01L3/0268 ,  B01L3/502707 ,  C12Q1/001 ,  G01N33/5438 ,  G01N35/0099 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0877 ,  B01L3/5088 ,  G01N2035/00059 ,  G01N2035/00237 ,  G01N2035/00455 ,  G01N2035/00534 ,  G01N2035/1034 ,  G01N2035/1076 ,  G01N35/109

摘要： A method of forming a permselective layer on preselected areas of a substantially planar sensing device is disclosed. The claimed method includes establishing and confining a liquid film, derived from a silane compound mixed in a suitable solvent, within a predetermined area of the sensing device. The process relates to photolithographic imaging and developing methods coupled to a film-curing step that provides a patterned permselective layer having the desired semipermeable characteristics.

摘要翻译： 公开了一种在基本上平面的感测装置的预选区域上形成选择渗透层的方法。 要求保护的方法包括建立并限制衍生自混合在合适溶剂中的硅烷化合物的液膜在感测装置的预定区域内。 该方法涉及耦合到膜固化步骤的光刻成像和显影方法，其提供具有所需半透性特征的图案化选择层。