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    METHOD OF REDUCING TEMPERATURE DIFFERENCE BETWEEN A PAIR OF SUBSTRATES AND FLUID REACTION DEVICE USING THE SAME
    4.
    发明申请
    METHOD OF REDUCING TEMPERATURE DIFFERENCE BETWEEN A PAIR OF SUBSTRATES AND FLUID REACTION DEVICE USING THE SAME 有权
    降低基板对流与使用其的流体反应装置之间的温度差异的方法

    公开(公告)号：US20080053647A1

    公开(公告)日：2008-03-06

    申请号：US11689640

    申请日：2007-03-22

    申请人： Kak Namkoong ,  Su-hyeon Kim ,  Jin-tae Kim ,  Chin-sung Park ,  Young-sun Lee

    发明人： Kak Namkoong ,  Su-hyeon Kim ,  Jin-tae Kim ,  Chin-sung Park ,  Young-sun Lee

    IPC分类号： F28F7/00

    CPC分类号： B81B3/0081 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00822 ,  B01J2219/00873 ,  B01L3/5027 ,  B01L7/5255 ,  B01L2200/14 ,  B01L2200/147 ,  B01L2300/1805 ,  B01L2300/1827 ,  B81B2201/051 ,  F28F21/02 ,  F28F2013/006

    摘要： A method of reducing a temperature difference between a high-temperature and a low-temperature substrate includes interposing a heat transfer facilitating layer which has a higher thermal conductivity than air and can hold particles between the substrates, and maintaining close contact between the high-temperature substrate, the heat transfer facilitating layer, and the low-temperature substrate, wherein formation of an air layer can be at least substantially prevented between the high-temperature substrate and the heat transfer facilitating layer, and between the low-temperature substrate and the heat transfer facilitating layer. A fluid reaction device includes a microfluidic reaction chip which accommodates a fluid, a heater, and a heat transfer facilitating layer which is interposed between the microfluidic reaction chip and the heater, the heat transfer facilitating layer has a higher thermal conductivity than air and can hold particles, wherein formation of an air layer can be prevented.

    摘要翻译： 降低高温和低温基板之间的温度差的方法包括：介于热传导率高于空气的传热促进层，并且可以在基板之间保持颗粒，并且保持高温 基板，传热促进层和低温基板，其中至少可以在高温基板和传热促进层之间以及在低温基板和热量之间基本上防止空气层的形成 转移促进层。 流体反应装置包括容纳流体的微流体反应芯片，加热器和介于微流体反应芯片和加热器之间的传热促进层，传热促进层具有比空气更高的导热性并且可以保持 颗粒，其中可以防止空气层的形成。

    MICROFLUIDIC DEVICE AND METHOD OF FABRICATING THE SAME
    5.
    发明申请
    MICROFLUIDIC DEVICE AND METHOD OF FABRICATING THE SAME 审中-公开
    微流体装置及其制造方法

    公开(公告)号：US20090170189A1

    公开(公告)日：2009-07-02

    申请号：US12184317

    申请日：2008-08-01

    申请人： Chin-sung PARK ,  Kak Namkoong ,  Young-sun Lee

    发明人： Chin-sung PARK ,  Kak Namkoong ,  Young-sun Lee

    IPC分类号： C12M1/34 ,  C12M1/00 ,  B32B37/06

    CPC分类号： C12M23/16 ,  B01L2200/12 ,  B01L2300/0816 ,  B01L2300/1827 ,  B29C65/02 ,  B29C65/08 ,  B29C66/1122 ,  B29C66/30223 ,  B29C66/47 ,  B29C66/53461 ,  B29C66/61 ,  B29C66/71 ,  B29C66/7352 ,  B29C66/73921 ,  B29C66/8322 ,  B29K2023/06 ,  B29K2023/12 ,  B29K2033/12 ,  B29K2067/00 ,  B29K2069/00 ,  B29L2031/756 ,  Y10T156/10 ,  B29K2067/003 ,  B29K2023/38

    摘要： Provided are a microfluidic device that performs a biochemical reaction using a small amount of a biochemical fluid and detects the result thereof, and a method of fabricating the same. The microfluidic device includes: a substrate which comprises a chamber that is formed as a concave groove and accommodates a fluid in the bottom surface of the substrate, and is formed of polymer; and a film welded on the bottom surface of the substrate to seal the chamber so that the chamber is not open at the bottom surface of the substrate, and formed of polymer. The method of fabricating a microfluidic device includes: preparing a substrate which comprises a chamber that is formed as a concave groove and accommodates a fluid in the bottom surface of the substrate, and is formed of polymer; and welding a film on a bottom surface of the substrate to seal the chamber so that the chamber is not opened at the bottom surface of the substrate, the film being formed of polymer.

    摘要翻译： 提供了使用少量生物化学流体进行生化反应并检测其结果的微流体装置及其制造方法。 微流体装置包括：基板，其包括形成为凹槽并容纳基板的底表面中的流体的腔室，并且由聚合物形成; 以及焊接在基板的底表面上的膜以密封室，使得室不在基板的底表面处开口，并且由聚合物形成。 制造微流体装置的方法包括：制备基板，其包括形成为凹槽的腔室，并且在基底的底表面容纳流体，并且由聚合物形成; 并且在所述基板的底表面上焊接膜以密封所述室，使得所述室在所述基板的底表面处不被打开，所述膜由聚合物形成。

    Method for quantifying initial concentration of nucleic acid from real-time nucleic acid amplification data
    9.
    发明申请
    Method for quantifying initial concentration of nucleic acid from real-time nucleic acid amplification data 有权

    公开(公告)号：US20060047443A1

    公开(公告)日：2006-03-02

    申请号：US11217694

    申请日：2005-09-01

    申请人： Kak Namkoong ,  Jin-tae Kim ,  Young-sun Lee ,  Young-a Kim

    发明人： Kak Namkoong ,  Jin-tae Kim ,  Young-sun Lee ,  Young-a Kim

    IPC分类号： G06F19/00 ,  C12Q1/68

    CPC分类号： C12Q1/6851

    摘要： Provided is a method for quantifying an initial concentration of a nucleic acid from a real-time nucleic acid amplification data. Nucleic acid (DNA or RNA) extracted from organism or virus is amplified using an enzyme. Then, the initial concentration of the nucleic acid is found by calculating the characteristic amplification cycle number or the characteristic amplification time at which the fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid has half of its maximum value, or the characteristic amplification cycle number or the characteristic amplification time at which the amplification efficiency has the maximum or the minimum value, or the prior-to-amplification fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid. Accordingly, the initial concentration of the nucleic acid can be calculated without differentiation or integration.

    Method for quantifying initial concentration of nucleic acid from real-time nucleic acid amplification data
    10.
    发明授权
    Method for quantifying initial concentration of nucleic acid from real-time nucleic acid amplification data 有权
    从实时核酸扩增数据定量核酸初始浓度的方法

    公开(公告)号：US08386191B2

    公开(公告)日：2013-02-26

    申请号：US13161788

    申请日：2011-06-16

    申请人： Kak Namkoong ,  Jin-tae Kim ,  Young-sun Lee ,  Young-a Kim

    发明人： Kak Namkoong ,  Jin-tae Kim ,  Young-sun Lee ,  Young-a Kim

    IPC分类号： G01N33/48

    CPC分类号： C12Q1/6851

    摘要： Provided is a method for quantifying an initial concentration of a nucleic acid from a real-time nucleic acid amplification data. Nucleic acid (DNA or RNA) extracted from organism or virus is amplified using an enzyme. Then, the initial concentration of the nucleic acid is found by calculating the characteristic amplification cycle number or the characteristic amplification time at which the fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid has half of its maximum value, or the characteristic amplification cycle number or the characteristic amplification time at which the amplification efficiency has the maximum or the minimum value, or the prior-to-amplification fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid. Accordingly, the initial concentration of the nucleic acid can be calculated without differentiation or integration.

    摘要翻译： 提供了从实时核酸扩增数据定量核酸的初始浓度的方法。 使用酶扩增从生物或病毒提取的核酸（DNA或RNA）。 然后，通过计算由核酸的背景荧光强度减去的核酸的荧光强度具有其最大值的一半的特征扩增循环数或特异性扩增时间来发现核酸的初始浓度，或 扩增效率具有由核酸的背景荧光强度减去的核酸的最大值或最小值或以前的扩增荧光强度的特征扩增循环数或特征扩增时间。 因此，可以在不分化或整合的情况下计算核酸的初始浓度。