利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

14 条记录 (耗时 0.031 秒)

    Recombinant microbial 3-hydroxybutyrate dehydrogenase a process for its production and the use thereof
    3.
    发明授权
    Recombinant microbial 3-hydroxybutyrate dehydrogenase a process for its production and the use thereof 失效
    重组微生物3-羟基丁酸脱氢酶一种生产方法及其应用

    公开(公告)号：US06255093B1

    公开(公告)日：2001-07-03

    申请号：US09287097

    申请日：1999-04-07

    申请人： Rainer Schmuck ,  Rainer Müller ,  Harald Weisser ,  Alfred Engel ,  Kerstin Krüger

    发明人： Rainer Schmuck ,  Rainer Müller ,  Harald Weisser ,  Alfred Engel ,  Kerstin Krüger

    IPC分类号： C12N904

    CPC分类号： C12N9/0006

    摘要： DNA molecule comprising a structural gene which codes for a bacterial protein with the enzymatic activity of 3-hydroxybutyrate dehydrogenase (3-HBDH), a recombinant DNA containing the DNA molecule, microorganisms transformed accordingly, a process for obtaining 3-HBDH by culturing a suitable transformed microorganism as well as a method for the determination of ketone bodies in the presence of the enzyme. A structural gene from Rhodobacter sphaeroides is preferred.

    摘要翻译： 包含编码具有3-羟基丁酸脱氢酶（3-HBDH）的酶活性的细菌蛋白质的结构基因的DNA分子，含有该DNA分子的重组DNA，相应地转化的微生物，通过培养合适的方法获得3-HBDH的方法 转化的微生物以及在酶存在下测定酮体的方法。 来自球形红细菌的结构基因是优选的。

    Soluble rubella E1 envelope protein variants
    4.
    发明授权
    Soluble rubella E1 envelope protein variants 有权
    可溶性风疹E1包膜蛋白变体

    公开(公告)号：US07604935B2

    公开(公告)日：2009-10-20

    申请号：US11584887

    申请日：2006-10-23

    申请人： Barbara Upmeier ,  Ralf Bollhagen ,  Alfred Engel ,  Elke Faatz ,  Peter Schaarschmidt ,  Christian Scholz ,  Toralf Zarnt

    发明人： Barbara Upmeier ,  Ralf Bollhagen ,  Alfred Engel ,  Elke Faatz ,  Peter Schaarschmidt ,  Christian Scholz ,  Toralf Zarnt

    IPC分类号： C12Q1/70 ,  C12N7/01 ,  A61K39/20

    CPC分类号： C07K14/005 ,  C07K2319/35 ,  C12N2770/36222 ,  Y10S530/826

    摘要： A soluble rubella E1 antigen variant is disclosed that comprises amino acids 334-409 of the native rubella E1 peptide, but lacks the C-terminal end and at least the transmembrane region and the anchor segment as well as at least the amino acids 143 to 164. Also described is a recombinant DNA molecule encoding the rubella E1 antigen variants which are recombinantly expressed as a chaperone fusion protein, refolded into a soluble and immunoreactive conformation, and further used for the serological detection of anti-rubella antibodies. In addition, also disclosed is a method for the detection, determination and quantification of anti-rubella antibodies of IgG and/or IgM subclass in a sample wherein the rubella E1 antigen is used as a capture reagent and/or binding partner for the antibodies.

    摘要翻译： 公开了可溶性风疹E1抗原变体，其包含天然风疹E1肽的氨基酸334-409，但缺少C末端和至少跨膜区和锚定区以及至少氨基酸143至164 还描述了编码风疹E1抗原变体的重组DNA分子，其被重组表达为伴侣融合蛋白，重折叠成可溶性和免疫反应性构象，并进一步用于抗风疹抗体的血清学检测。 此外，还公开了用于检测，测定和定量样品中IgG和/或IgM亚类的抗风疹抗体的方法，其中所述风疹E1抗原用作抗体的捕获试剂和/或结合配偶体。

    Erythropoietin conjugates
    5.
    发明授权
    Erythropoietin conjugates 有权
    红细胞生成素共轭物

    公开(公告)号：US07128913B2

    公开(公告)日：2006-10-31

    申请号：US10014363

    申请日：2001-12-11

    申请人： Josef Burg ,  Alfred Engel ,  Reinhard Franze ,  Bernd Hilger ,  Hartmut Ernst Schurig ,  Wilhelm Tischer ,  Manfred Wozny

    发明人： Josef Burg ,  Alfred Engel ,  Reinhard Franze ,  Bernd Hilger ,  Hartmut Ernst Schurig ,  Wilhelm Tischer ,  Manfred Wozny

    IPC分类号： A61K39/385 ,  C07K1/00 ,  A61K35/14

    CPC分类号： A61K47/62 ,  A61K47/60

    摘要： The present invention refers to conjugates of erythropoietin with poly(ethylene glycol) comprising an erythropoietin glycoprotein having an N-terminal α-amino group and having the in vivo biological activity of causing bone marrow cells to increase production of reticulocytes and red blood cells and selected from the group consisting of human erythropoietin and analogs thereof which have the sequence of human erythropoietin modified by the addition of from 1 to 6 glycosylation sites or a rearrangement of at least one glycosylation site; said glycoprotein being covalently linked to one poly(ethylene glycol) group of the formula —CO—(CH2)x—(OCH2CH2)m—OR wherein the —CO of the poly(ethylene glycol) group forms an amide bond with said N-terminal α-amino group; and wherein R is lower alkyl; x is 2 or 3; and m is from about 450 to about 1350.

    摘要翻译： 本发明涉及红细胞生成素与聚（乙二醇）的缀合物，其包含具有N-末端α-氨基的促红细胞生成素糖蛋白，并且具有引起骨髓细胞增加网织红细胞和红细胞产生的体内生物学活性并选择 由人红细胞生成素及其类似物组成，其具有通过加入1至6个糖基化位点或至少一个糖基化位点的重排而修饰的促红细胞生成素序列; 所述糖蛋白共价连接到一个式（Ⅳ）的聚（乙二醇）基团，其中：-in-line-formula description =“In-line Formulas”end =“lead”→CO-（CH 2 >） - （OCH 2 CH 2）m -OR <βin-line-formula description = “直链式”末端=“尾”→其中聚（乙二醇）基团的-CO与所述N-末端α-氨基形成酰胺键; 并且其中R是低级烷基; x为2或3; m为约450至约1350。

    Soluble rubella E1 envelope protein variants
    8.
    发明申请
    Soluble rubella E1 envelope protein variants 有权
    可溶性风疹E1包膜蛋白变体

    公开(公告)号：US20070154883A1

    公开(公告)日：2007-07-05

    申请号：US11584887

    申请日：2006-10-23

    申请人： Barbara Upmeier ,  Ralf Bollhagen ,  Alfred Engel ,  Elke Faatz ,  Peter Schaarschmidt ,  Christian Scholz ,  Toralf Zarnt

    发明人： Barbara Upmeier ,  Ralf Bollhagen ,  Alfred Engel ,  Elke Faatz ,  Peter Schaarschmidt ,  Christian Scholz ,  Toralf Zarnt

    IPC分类号： C12Q1/70

    CPC分类号： C07K14/005 ,  C07K2319/35 ,  C12N2770/36222 ,  Y10S530/826

    摘要： The invention disclosed relates to a soluble rubella E1 antigen and variants of this peptide characterized by lacking at the C-terminal end at least the transmembrane region and the anchor segment as well as at least the amino acids 143 to 164 and containing at least the region spanning the disulfide bridges Cys 349-Cys 352 and Cys 368-401 whereas the N-terminus (Cys 349) of this region contains additionally at least 15 amino acids and/or the C-terminus (Cys 401) of this region contains additionally at least 8 amino acids of the adjacent rubella E1 antigen sequence. Also described are a recombinant DNA molecule encoding the rubella E1 antigen and variants which are recombinantly expressed as a chaperone fusion protein, refolded into a soluble and immunoreactive conformation, and further used for the serological detection of anti-rubella antibodies. In addition, also disclosed is a method for the detection, determination and quantification of anti-rubella antibodies of IgG and/or IgM subclass in a sample wherein the rubella E1 antigen is used as a capture reagent and/or binding partner for the antibodies.

    摘要翻译： 所公开的本发明涉及可溶性风疹E1抗原和该肽的变体，其特征在于至少在C-末端缺少至少跨膜区和锚段以及至少氨基酸143至164并且至少含有该区域 跨越二硫键Cys 349-Cys 352和Cys 368-401，而该区域的N末端（Cys 349）另外包含至少15个氨基酸和/或该区域的C末端（Cys 401） 相邻风疹E1抗原序列的至少8个氨基酸。 还描述了编码风疹E1抗原的重组DNA分子和重组表达为伴侣融合蛋白的变体，重新折叠成可溶性和免疫反应性构象，并进一步用于抗风疹抗体的血清学检测。 此外，还公开了用于检测，测定和定量样品中IgG和/或IgM亚类的抗风疹抗体的方法，其中所述风疹E1抗原用作抗体的捕获试剂和/或结合配偶体。

    Farm machines having flexible skirts for moving agricultural products on the ground
    10.
    发明授权
    Farm machines having flexible skirts for moving agricultural products on the ground 失效
    农机具有灵活的裙子，用于将农产品在地面上移动

    公开(公告)号：US4693065A

    公开(公告)日：1987-09-15

    申请号：US826815

    申请日：1986-02-06

    申请人： Jerome Aron ,  Alfred Engel

    发明人： Jerome Aron ,  Alfred Engel

    IPC分类号： A01D78/10 ,  A01D84/02 ,  A01D80/00 ,  A01D84/00

    CPC分类号： A01D84/02 ,  Y10S56/21

    摘要： A farm machine includes a frame, at least one rotor mounted on the frame, and a tapered flexible skirt for moving products that are on the ground mounted on the rotor. The tapered flexible skirt has close to its outer edge a zone the thickness of which is greater than the thickness of the remaining part of the tapered flexible skirt. The zone is an integral part of the tapered flexible skirt and extends without interruption over the entire periphery of the tapered flexible skirt.

    摘要翻译： 农机包括框架，安装在框架上的至少一个转子和用于移动安装在转子上的地面上的产品的锥形柔性裙部。 锥形柔性裙部靠近其外边缘具有其厚度大于锥形柔性裙部的剩余部分的厚度的区域。 该区域是锥形柔性裙部的整体部分，并且在锥形柔性裙部的整个周边上不间断地延伸。