公开(公告)号：US12270068B2

公开(公告)日：2025-04-08

申请号：US17793926

申请日：2021-01-27

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Aydogan Ozcan ,  Yair Rivenson ,  Hongda Wang ,  Hatice Ceylan Koydemir ,  Yunzhe Qiu

IPC: C12Q1/04 ,  G02B21/26 ,  G02B21/36 ,  G03H1/00 ,  G06V10/10 ,  G06V10/82 ,  G06V20/69 ,  H04N23/56 ,  H04N23/698

Abstract: A system for the detection and classification of live microorganisms in a sample includes a light source and an incubator holding one or more sample-containing growth plates. A translation stage moves the image sensor and/or the growth plate(s) along one or more dimensions to capture time-lapse holographic images of microorganisms. Image processing software executed by a computing device captures time-lapse holographic images of the microorganisms or clusters of microorganisms on the one or more growth plates. The image processing software is configured to detect candidate microorganism colonies in reconstructed, time-lapse holographic images based on differential image analysis. The image processing software includes one or more trained deep neural networks that process the time-lapsed image(s) of candidate microorganism colonies to detect true microorganism colonies and/or output a species associated with each true microorganism colony.

公开(公告)号：US20210374381A1

公开(公告)日：2021-12-02

申请号：US17285898

申请日：2019-10-18

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Aydogan Ozcan ,  Yibo Zhang ,  Hatice Ceylan Koydemir

IPC: G06K9/00 ,  G06K9/66 ,  G06T7/20 ,  G01N15/14 ,  G01N33/49 ,  G03H1/00 ,  G03H1/08

Abstract: Systems and methods for detecting motile objects (e.g., parasites) in a fluid sample by utilizing the locomotion of the parasites as a specific biomarker and endogenous contrast mechanism. The imaging platform includes one or more substantially optically transparent sample holders. The imaging platform has a moveable scanning head containing light sources and corresponding image sensor(s) associated with the light source(s). The light source(s) are directed at a respective sample holder containing a sample and the respective image sensor(s) are positioned below a respective sample holder to capture time-varying holographic speckle patterns of the sample contained in the sample holder. The image sensor(s). A computing device is configured to receive time-varying holographic speckle pattern image sequences obtained by the image sensor(s). The computing device generates a 3D contrast map of motile objects within the sample use deep learning-based classifier software to identify the motile objects.

公开(公告)号：US10175215B2

公开(公告)日：2019-01-08

申请号：US15538309

申请日：2015-12-21

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Aydogan Ozcan ,  Bingen Cortazar ,  Hatice Ceylan Koydemir ,  Derek Tseng ,  Steve Feng

IPC: G01N21/25 ,  G01N33/00 ,  H04Q9/00 ,  G01N21/27 ,  G01N21/31 ,  G06T7/90 ,  G06K9/00 ,  G06K9/46 ,  G06K9/62 ,  G01N21/84 ,  G01N21/01 ,  G06K9/20 ,  G06K9/22

Abstract: A system for measuring chlorophyll concentration in a leaf sample includes a leaf-holding illuminator device with a main body containing a power source, a plurality of switchable light sources emitting light at different spectra (e.g., red and white light from a broadband light source), and a cap detachably secured to the main body using one or more fastening means. The leaf sample is interposed between the main body and the cap and held in place during imaging. The system includes a mobile electronic device having a camera configured to capture an image of the leaf illuminated by the plurality of switchable light sources, the mobile electronic device having wireless connectivity to a network and an application contained therein configured to transfer the images to a remote sever or computer via the network for data processing. A final chlorophyll index value is calculated based on the transferred images.

公开(公告)号：US11893779B2

公开(公告)日：2024-02-06

申请号：US17285898

申请日：2019-10-18

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Aydogan Ozcan ,  Yibo Zhang ,  Hatice Ceylan Koydemir

IPC: G03H1/00 ,  G06T7/20 ,  G06V20/69 ,  G06V30/19 ,  G06V10/82 ,  G01N15/14 ,  G01N33/49 ,  G03H1/08

CPC classification number: G06V10/82 ,  G01N15/14 ,  G01N33/49 ,  G03H1/0005 ,  G03H1/08 ,  G06T7/20 ,  G06V20/69 ,  G06V20/698 ,  G06V30/19173 ,  G01N2015/1486 ,  G03H2001/0033 ,  G03H2210/42 ,  G03H2210/46 ,  G06T2207/20081 ,  G06T2207/30024 ,  G06T2207/30242

Abstract: Systems and methods for detecting motile objects (e.g., parasites) in a fluid sample by utilizing the locomotion of the parasites as a specific biomarker and endogenous contrast mechanism. The imaging platform includes one or more substantially optically transparent sample holders. The imaging platform has a moveable scanning head containing light sources and corresponding image sensor(s) associated with the light source(s). The light source(s) are directed at a respective sample holder containing a sample and the respective image sensor(s) are positioned below a respective sample holder to capture time-varying holographic speckle patterns of the sample contained in the sample holder. The image sensor(s). A computing device is configured to receive time-varying holographic speckle pattern image sequences obtained by the image sensor(s). The computing device generates a 3D contrast map of motile objects within the sample use deep learning-based classifier software to identify the motile objects.

公开(公告)号：US20230060037A1

公开(公告)日：2023-02-23

申请号：US17793926

申请日：2021-01-27

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Aydogan Ozcan ,  Yair Rivenson ,  Hongda Wang ,  Hatice Ceylan Koydemir ,  Yunzhe Qiu

IPC: G06V20/69 ,  H04N5/232 ,  G06V10/10 ,  G06V10/82 ,  H04N5/225 ,  C12Q1/04 ,  G02B21/36 ,  G02B21/26 ,  G03H1/00

Abstract: A system for the detection and classification of live microorganisms in a sample includes a light source and an incubator holding one or more sample-containing growth plates. A translation stage moves the image sensor and/or the growth plate(s) along one or more dimensions to capture time-lapse holographic images of microorganisms. Image processing software executed by a computing device captures time-lapse holographic images of the microorganisms or clusters of microorganisms on the one or more growth plates. The image processing software is configured to detect candidate microorganism colonies in reconstructed, time-lapse holographic images based on differential image analysis. The image processing software includes one or more trained deep neural networks that process the time-lapsed image(s) of candidate microorganism colonies to detect true microorganism colonies and/or output a species associated with each true microorganism colony.

公开(公告)号：US20220268754A1

公开(公告)日：2022-08-25

申请号：US17627400

申请日：2020-07-29

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Aydogan Ozcan ,  Kevin de Haan ,  Hatice Ceylan Koydemir ,  Sabiha Tok ,  Derek Tseng

IPC: G01N33/18 ,  G01N21/64 ,  G02B6/04 ,  F21V8/00 ,  G01N21/78

Abstract: A system for detecting the presence of E. coli and total coliform in a water sample includes a sample holder that holds smaller, divided volumes of the sample and a testing reagent. A plurality of light sources are disposed above sample holder. The divided sample volumes are are illuminated with first and second light sources emitting light at different wavelengths. A bundle of optical fibers is provided with having an input end located adjacent to the divided sample volumes and is configured to receive light passing through the sample volumes. Light is output from the bundle of optical fibers and is captured with a camera. Image processing software is provided and is configured to calculate a light intensity in first and second wavelength channels at different times and outputs a positive/negative indication for E. coli and total coliform for the water sample.

公开(公告)号：US20180003686A1

公开(公告)日：2018-01-04

申请号：US15538309

申请日：2015-12-21

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Aydogan Ozcan ,  Bingen Cortazar ,  Hatice Ceylan Koydemir ,  Derek Tseng ,  Steve Feng

IPC: G01N33/00 ,  G01N21/84 ,  G06K9/00 ,  G06K9/20 ,  G06K9/62 ,  G01N21/27 ,  G01N21/31 ,  G06K9/46 ,  G06K9/22 ,  G01N21/01

CPC classification number: G01N33/0098 ,  G01N21/274 ,  G01N21/31 ,  G01N21/84 ,  G01N2021/0125 ,  G01N2021/8466 ,  G01N2201/0221 ,  G01N2201/0627 ,  G01N2201/0634 ,  G06K9/00657 ,  G06K9/2027 ,  G06K9/2054 ,  G06K9/22 ,  G06K9/4661 ,  G06K9/6202 ,  G06T7/90 ,  H04Q9/00

Abstract: A system for measuring chlorophyll concentration in a leaf sample includes a leaf-holding illuminator device with a main body containing a power source, a plurality of switchable light sources emitting light at different spectra (e.g., red and white light from a broadband light source), and a cap detachably secured to the main body using one or more fastening means. The leaf sample is interposed between the main body and the cap and held in place during imaging. The system includes a mobile electronic device having a camera configured to capture an image of the leaf illuminated by the plurality of switchable light sources, the mobile electronic device having wireless connectivity to a network and an application contained therein configured to transfer the images to a remote sever or computer via the network for data processing. A final chlorophyll index value is calculated based on the transferred images.