公开(公告)号：US20140333453A1

公开(公告)日：2014-11-13

申请号：US14339069

申请日：2014-07-23

申请人： The Trustees of Columbia University in the City of New York

发明人： Samuel K. SIA ,  Mario Matteo MODENA ,  Paolo CADINU ,  Keith YEAGER ,  Yuk Kee Cheung POH ,  Robert HOUGHTALING ,  Tassaneewan LAKSANASOPIN ,  Curtis D. CHIN

IPC分类号： G06F19/00

CPC分类号： G06F19/3418 ,  G06F19/00 ,  G06F21/602 ,  G16H10/60 ,  H04L9/14

摘要： A portable unitary device handheld diagnostic device can be operated with minimal power requirement and provides ease of operation as well as low cost communication of diagnostic data from remote locations. The device can provide nucleic-acid based diagnostics with minimal training, little to no sample preparation, and generates diagnostic data in about 45 minutes. A system can enable point of care transmission from any location globally using a low cost satellite-based data link technique, for example, Short Burst Data (SBD), combined with data encoding.

摘要翻译： 便携式单一设备手持诊断设备可以以最小的功率需求进行操作，并提供易于操作以及来自远程位置的诊断数据的低成本通信。 该设备可以提供基于核酸的诊断，具有最少的培训，很少甚至无需样品制备，并在约45分钟内产生诊断数据。 系统可以使用低成本的基于卫星的数据链路技术（例如，与数据编码相结合的短突发数据（SBD））来全球从任何位置发射护理点。

公开(公告)号：US20230274538A1

公开(公告)日：2023-08-31

申请号：US18125341

申请日：2023-03-23

申请人： The Trustees of Columbia University in the City of New York

发明人： Samuel K. SIA ,  Shih-Fu CHANG ,  Siddarth ARUMUGAM ,  Uzay MACAR ,  Jiawei MA ,  Guangxing HAN ,  David COLBURN

IPC分类号： G06V10/82 ,  G06V10/77

CPC分类号： G06V10/82 ,  G06V10/7715

摘要： A framework for a few-shot learning method is disclosed. In a first part, self-supervision and classification supervision are used to train a feature extractor. An example self-supervision method comprises running grayscale images through an edge filter, normalizing the filtered images, setting the normalized images to ground truth, generating feature-extracted images, using a decoder to reconstruct images from the feature-extracted images, determining a loss between the reconstructed images and the ground truth images, and using the loss to update parameters of the feature extractor. In a second part, a few-shot adaptation process is performed to adapt the model to a novel rapid test kit.

公开(公告)号：US20160029937A1

公开(公告)日：2016-02-04

申请号：US14779556

申请日：2014-03-26

申请人： THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

发明人： Samuel K. SIA ,  Nalin TEJAVIBULYA

IPC分类号： A61B5/15 ,  A61B5/00 ,  A61B5/145 ,  A61B5/1459 ,  A61B5/151

CPC分类号： A61B5/150984 ,  A61B5/14546 ,  A61B5/1459 ,  A61B5/14865 ,  A61B5/15003 ,  A61B5/150099 ,  A61B5/150229 ,  A61B5/150389 ,  A61B5/150503 ,  A61B5/150755 ,  A61B5/15087 ,  A61B5/15123 ,  A61B5/1513 ,  A61B5/15153 ,  A61B5/15163 ,  A61B5/489 ,  A61B5/6831 ,  A61B5/685 ,  A61M37/0015 ,  A61M2037/0023 ,  A61M2037/003 ,  A61M2037/0046 ,  A61M2037/0061

摘要： Devices systems and methods for fluid extraction and delivery to human or animal hosts are described. In embodiments, microneedles are employed to provide low infection risk and painless access to and administration of fluids. The disclosed embodiments address, among others, issues of cost, portability, ease of use in remote settings including use by untrained personnel, and others.

摘要翻译： 描述了用于流体提取和递送到人或动物宿主的装置系统和方法。 在实施方案中，使用微针提供低感染风险并且无痛地进入和施用流体。 所公开的实施例尤其涉及成本，便携性，在远程设置中的易用性，包括未经培训的人员等的使用的问题。

公开(公告)号：US20180042970A1

公开(公告)日：2018-02-15

申请号：US15694152

申请日：2017-09-01

申请人： THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

发明人： Ninna S. ROSSEN ,  Samuel K. SIA ,  Brian M. GILLETTE

IPC分类号： A61K35/44 ,  A61K9/50 ,  A61K35/28 ,  A61K9/00 ,  C12N5/071

CPC分类号： A61K35/44 ,  A61K9/0019 ,  A61K9/5063 ,  A61K35/28 ,  C12N5/0691 ,  C12N5/0697 ,  C12N2501/02 ,  C12N2501/115 ,  C12N2501/165 ,  C12N2501/33 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2501/395 ,  C12N2501/81 ,  C12N2502/1352 ,  C12N2502/1358 ,  C12N2502/28 ,  C12N2539/00

摘要： Spheroid microtissues that can mimic native tissue-like structure and function, spheroid production methods that are high-throughput, suitable for efficient production, maintainable over long-term culture, and/or offer repeatable control over size distribution. Spheroids that have blood vessels, including spheroids with functional, blood-perfused vascular networks upon injection in vivo. Dissolvable hydrogel microwell arrays for high throughput parallel formation of spheroids in a single pipetting step and easy retrieval for downstream applications. A method to produce prevascularized microtissues in sufficient numbers to form a macrotissue in vivo for therapeutic purposes. This method is based on sacrificial release of dissolvable microwell templates, a novel and scalable strategy which enables gentle harvesting of microtissues with control over size and composition. The method forms microtissues containing endothelial cells and mesenchymal stem cells, which are co-cultured under dynamic conditions and self-organize into blood-vessel units.

公开(公告)号：US20170227537A1

公开(公告)日：2017-08-10

申请号：US15503512

申请日：2015-08-13

申请人： The Trustees of Columbia University in the City of New York

发明人： Tiffany GUO ,  Samuel K. SIA ,  Jeisun Shi XIE ,  Fanxing MENG ,  Keith YEAGER

IPC分类号： G01N33/543 ,  G01N33/569 ,  G01N33/58 ,  G01N33/66 ,  G01N33/68 ,  B01L3/00 ,  G01N33/571

CPC分类号： G01N33/54366 ,  B01L3/502715 ,  B01L3/50273 ,  B01L2200/16 ,  B01L2300/023 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2400/0481 ,  B01L2400/049 ,  G01N33/56988 ,  G01N33/571 ,  G01N33/58 ,  G01N33/66 ,  G01N33/6827

摘要： Diagnostic systems, methods, and devices employing low-cost handheld components are disclosed herein. A diagnostic system can include a diagnostic device that is configured to perform one or more assays on a fluid sample, such as a whole blood sample, in one or more microfluidic channels or chambers. The diagnostic device can move the fluid sample into or through the one or more microfluidic channels or chambers without using any electrical power, for example, using manual actuation to generate a positive or negative pressure within the diagnostic device. The diagnostic device can have a connector for interfacing with a separate handheld unit that can provide power and data processing. For example, the separate handheld unit can be a smartphone or PDA, and the connector can interface with an existing input/output port of the unit to draw power and/or transmit data.

公开(公告)号：US20230203566A1

公开(公告)日：2023-06-29

申请号：US17986121

申请日：2022-11-14

申请人： The Trustees of Columbia University in the City of New York ,  Rover Diagnostics, Inc.

发明人： Samuel K. SIA ,  Nicole R. BLUMENFELD ,  Mark FASCIANO ,  Bulent ONALIR ,  Martin JASPAN

IPC分类号： C12Q1/6816 ,  C12Q1/6876 ,  C12Q1/686

CPC分类号： C12Q1/6816 ,  C12Q1/6876 ,  C12Q1/686 ,  C12Q2600/16

摘要： Methods and apparatus for rapid and accurate detection of nucleic acid in a single reaction chamber are provided. In one aspect, a patient specimen suspected of comprising a first nucleic acid is used to form a crude lysate which is combined with an infrared absorbing material, a detecting nucleic acid, and at least one reporter molecule in the single reaction chamber and heated by irradiating the reaction mixture with infrared light. Another aspect is directed to an apparatus for detecting a presence or absence of a plurality of different molecules within a reaction container. The apparatus comprises an infrared light source aimed to illuminate contents of the reaction container; an excitation light source positioned to illuminate contents of the reaction container; and a spectrometer positioned to detect emission light emanating from the reaction container.

公开(公告)号：US20210340595A1

公开(公告)日：2021-11-04

申请号：US17210083

申请日：2021-03-23

申请人： The Trustees of Columbia University in the City of New York

发明人： Samuel K. SIA ,  Nicole R. BLUMENFELD ,  Rodrigo C. CHAVES ,  Giuseppe MODICA ,  Michael LUETOLF

IPC分类号： C12Q1/6806 ,  C12N15/10

摘要： Aspects described herein provide blood sample purification and microfluidic nucleic acid amplification systems and methods for purifying blood samples and amplifying a target nucleic acid on the same system. The described systems and methods include a filter component and a microfluidic amplification component for amplification of nucleic acid from the blood sample, and can be used, for example, for point of care blood analysis.

公开(公告)号：US20130203157A1

公开(公告)日：2013-08-08

申请号：US13830605

申请日：2013-03-14

申请人： The Trustees of Columbia University in the City of New York

发明人： Yuk Kee CHEUNG ,  Samuel K. SIA ,  Curtis D. CHIN ,  Neha AGARWAL

IPC分类号： G01N33/569

CPC分类号： G01N33/56977 ,  G01N15/0612 ,  G01N15/1484 ,  G01N2015/008 ,  Y10T436/2575

摘要： A particle counter for analyzing blood has features which provide for automatic operation and preferably, also provide for portable use in a low resource setting. In a preferred embodiment, preferred embodiment, the device is used to obtain CD4 counts for AIDS diagnosis.

摘要翻译： 用于分析血液的颗粒计数器具有提供自动操作的特征，并且优选地还提供在低资源设置中的便携式使用。 在优选实施方案中，优选的实施方案中，该装置用于获得艾滋病诊断的CD4计数。

公开(公告)号：US20210378529A1

公开(公告)日：2021-12-09

申请号：US17400485

申请日：2021-08-12

申请人： The Trustees of Columbia University in the City of New York

发明人： David COLBURN ,  Samuel K. SIA

IPC分类号： A61B5/021 ,  A61B5/00

摘要： The disclosed subject matter includes a wearable device for blood pressure monitoring. The embodiments employ a set of sensors to calculate a relative external pressure. A transmural pressure error can be calculated based on the relative external pressure. A measured transmural pressure can be corrected based on the transmural pressure error. Some embodiments track altitude to calculate relative external pressure error. Some embodiments track arm orientation to calculate relative external pressure error.

公开(公告)号：US20210219852A1

公开(公告)日：2021-07-22

申请号：US17204352

申请日：2021-03-17

申请人： The Trustees of Columbia University in the City of New York

发明人： David COLBURN ,  Samuel K. SIA

IPC分类号： A61B5/021 ,  A61B5/00 ,  A61B8/08 ,  A61B5/055 ,  A61B5/0245 ,  A61B5/024

摘要： The disclosed subject matter includes a wearable device for cuffless blood pressure monitoring that does not require external per-person calibration, such as with a cuff-based measurement device. The embodiment employs photoplethysmography sensors to obtain pulse wave velocity and develops compensation for external pressure influences.