公开(公告)号：US20240001325A1

公开(公告)日：2024-01-04

申请号：US18345869

申请日：2023-06-30

Applicant: Twist Bioscience Corporation

Inventor： Eugene P. MARSH ,  Pierre F. INDERMUHLE ,  Bill James PECK

IPC: B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00722 ,  B01J2219/00596 ,  B01J2219/00533 ,  B01J2219/00317 ,  B01J2219/00378 ,  B01J2219/00662 ,  B01J2219/00637 ,  B01J2219/00653 ,  B01J2219/00509 ,  B01J2219/00716 ,  B01J2219/00659

Abstract: Devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Nano-scale devices allow for selective control over reaction conditions. Further, methods and devices described herein allow for the rapid construction of large libraries of highly accurate nucleic acids.

公开(公告)号：US20160310927A1

公开(公告)日：2016-10-27

申请号：US15135434

申请日：2016-04-21

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： William BANYAI ,  Bill James PECK ,  Andres FERNANDEZ ,  Siyuan CHEN ,  Pierre INDERMUHLE ,  Eugene P. MARSH

IPC: B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00497 ,  B01J2219/00511 ,  B01J2219/00522 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00662 ,  B01J2219/00722 ,  B01L3/50857 ,  C12N15/1093 ,  C12Q2563/159 ,  C12Q2565/513 ,  C12Q2565/629

Abstract: Devices and methods for de novo synthesis of large and highly accurate libraries of oligonucleic acids are provided herein. Devices include structures having a main channel and microchannels, where the microchannels have a high surface area to volume ratio. Devices disclosed herein provide for de novo synthesis of oligonucleic acids having a low error rate.

Abstract translation: 本文提供了用于从头合成大量和高精度的寡核酸文库的装置和方法。 器件包括具有主通道和微通道的结构，其中微通道具有高表面积与体积比。 本文公开的装置提供具有低错误率的寡核酸的从头合成。

公开(公告)号：US20230338913A1

公开(公告)日：2023-10-26

申请号：US18319356

申请日：2023-05-17

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： William BANYAI ,  Bill James PECK ,  Andres FERNANDEZ ,  Siyuan CHEN ,  Pierre INDERMUHLE ,  Eugene P. MARSH

IPC: B01J19/00 ,  C12N15/10

CPC classification number: B01J19/0046 ,  C12N15/1093 ,  B01J2219/00596 ,  B01J2219/00722 ,  B01J2219/00497 ,  B01J2219/00585 ,  B01J2219/00317 ,  B01J2219/00511 ,  B01J2219/00522 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00662 ,  B01L3/50857

Abstract: Devices and methods for de novo synthesis of large and highly accurate libraries of oligonucleic acids are provided herein. Devices include structures having a main channel and microchannels, where the microchannels have a high surface area to volume ratio. Devices disclosed herein provide for de novo synthesis of oligonucleic acids having a low error rate.

公开(公告)号：US20190240636A1

公开(公告)日：2019-08-08

申请号：US16384678

申请日：2019-04-15

Applicant: Twist Bioscience Corporation

Inventor： Bill James PECK ,  Pierre INDERMUHLE ,  Eugene P. MARSH ,  Andres FERNANDEZ ,  David STERN

IPC: B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00432 ,  B01J2219/00441 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00619 ,  B01J2219/00637 ,  B01J2219/00693 ,  B01J2219/00711 ,  B01J2219/00722

Abstract: Compositions, devices, methods and systems are provided for differential functionalization of a surface of a structure to support biopolymer synthesis. Provided herein are processes which include use of lamps, lasers, and/or microcontact printing to add functional groups to surfaces for the efficient and uniform synthesis of oligonucleic acids.

公开(公告)号：US20190118154A1

公开(公告)日：2019-04-25

申请号：US16165952

申请日：2018-10-19

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： Eugene P. MARSH ,  Pierre F. INDERMUHLE ,  Bill James PECK

IPC: B01J19/00

Abstract: Devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Nano-scale devices allow for selective control over reaction conditions. Further, methods and devices described herein allow for the rapid construction of large libraries of highly accurate nucleic acids.

公开(公告)号：US20180236425A1

公开(公告)日：2018-08-23

申请号：US15960319

申请日：2018-04-23

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： William BANYAI ,  Bill James PECK ,  Andres FERNANDEZ ,  Siyuan CHEN ,  Pierre INDERMUHLE ,  Eugene P. MARSH

IPC: B01J19/00 ,  B01L3/00

CPC classification number: B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00497 ,  B01J2219/00511 ,  B01J2219/00522 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00662 ,  B01J2219/00722 ,  B01L3/50857 ,  C12N15/1093 ,  C12Q2563/159 ,  C12Q2565/513 ,  C12Q2565/629

Abstract: Devices and methods for de novo synthesis of large and highly accurate libraries of oligonucleic acids are provided herein. Devices include structures having a main channel and microchannels, where the microchannels have a high surface area to volume ratio. Devices disclosed herein provide for de novo synthesis of oligonucleic acids having a low error rate.

公开(公告)号：US20180104664A1

公开(公告)日：2018-04-19

申请号：US15835342

申请日：2017-12-07

Applicant: Twist Bioscience Corporation

Inventor： Andres FERNANDEZ ,  Pierre INDERMUHLE ,  Eugene P. MARSH ,  William BANYAI ,  Bill James PECK

IPC: B01J19/00 ,  C12N15/10

CPC classification number: B01J19/0046 ,  B01J2219/00497 ,  B01J2219/00587 ,  B01J2219/00596 ,  B01J2219/00722 ,  C12N15/10 ,  C12N15/1093 ,  C40B50/00 ,  C40B50/14 ,  C40B50/18

Abstract: Methods, devices and systems are provided herein for surfaces for de novo polynucleotide synthesis that provide for increased polynucleotide yield. Surfaces described herein comprise a texture that increases surface area provide for increased polynucleotide yield compared to non-textured surfaces. In addition, the patterned placement of nucleoside coupling reagent spanning such surfaces provides for improved synthesis yield, representation, and a reduction in contamination on the surface between different polynucleotide species.

公开(公告)号：US20230331765A1

公开(公告)日：2023-10-19

申请号：US18319405

申请日：2023-05-17

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： Pierre F. INDERMUHLE ,  Eugene P. MARSH ,  Andres FERNANDEZ ,  William BANYAI ,  Bill J. PECK

IPC: C07H21/04 ,  B01J19/00 ,  C07H1/00 ,  C12P19/34 ,  C23C16/455

CPC classification number: C07H21/04 ,  B01J19/0046 ,  C07H1/00 ,  C12P19/34 ,  C23C16/455 ,  B01J2219/0059 ,  B01J2219/00527 ,  B01J2219/00596

Abstract: Methods and devices are provided herein for surfaces for de novo nucleic acid synthesis which provide for low error rates. In addition, methods and devices are provided herein for increased nucleic acid mass yield resulting from de novo nucleic acid synthesis.

公开(公告)号：US20210170356A1

公开(公告)日：2021-06-10

申请号：US17180614

申请日：2021-02-19

Applicant: Twist Bioscience Corporation

Inventor： Bill James PECK ,  Pierre INDERMUHLE ,  Eugene P. MARSH ,  Andres FERNANDEZ ,  David STERN

IPC: B01J19/00

Abstract: Compositions, devices, methods and systems are provided for differential functionalization of a surface of a structure to support biopolymer synthesis. Provided herein are processes which include use of lamps, lasers, and/or microcontact printing to add functional groups to surfaces for the efficient and uniform synthesis of oligonucleic acids.

公开(公告)号：US20200330953A1

公开(公告)日：2020-10-22

申请号：US16921712

申请日：2020-07-06

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： William BANYAI ,  Bill James PECK ,  Andres FERNANDEZ ,  Siyuan CHEN ,  Pierre INDERMUHLE ,  Eugene P. MARSH

IPC: B01J19/00 ,  C12N15/10

Abstract: Devices and methods for de novo synthesis of large and highly accurate libraries of oligonucleic acids are provided herein. Devices include structures having a main channel and microchannels, where the microchannels have a high surface area to volume ratio. Devices disclosed herein provide for de novo synthesis of oligonucleic acids having a low error rate.