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149 条记录 (耗时 0.033 秒)

    Fluorometry and fluorometric device and substrate suitable therefor
    5.
    发明授权
    Fluorometry and fluorometric device and substrate suitable therefor 失效
    荧光测定法和荧光测定装置及适用的底物

    公开(公告)号：US06963397B2

    公开(公告)日：2005-11-08

    申请号：US09764049

    申请日：2001-01-19

    申请人： Tomohiro Suzuki ,  Tadashi Okamoto ,  Kazuhiro Matsumoto ,  Nobuko Yamamoto

    发明人： Tomohiro Suzuki ,  Tadashi Okamoto ,  Kazuhiro Matsumoto ,  Nobuko Yamamoto

    IPC分类号： G01J3/44 ,  G01N21/64 ,  G01N33/483

    CPC分类号： G01N21/6458 ,  G01J3/4406 ,  G01N21/6428

    摘要： To provide a method of efficiently removing excitation light in a device for measuring fluorescence emitted from samples on a measuring surface of a substrate while illuminating the samples with excitation light.The method is a fluorometry characterized in that an excitation light illumination portion where the samples are illuminated with the excitation light and a light detecting portion where measurements are made of the fluorescence are placed in such a manner as to make it possible to prevent the excitation light from approaching the light detecting portion, and measurements of the fluorescence emitted from the samples on the measuring surface of the substrate are made in such a manner as to relatively move the samples from the excitation light illumination portion to the light detecting portion after illuminating the same with the excitation light.

    摘要翻译： 提供一种在用激发光照射样品的同时在基板的测量表面上测量从样品发射的荧光的装置中有效地去除激发光的方法。 该方法是一种荧光测定法，其特征在于，其中用激发光照射样品的激发光照射部分和其中测量荧光的光检测部分以使得可以防止激发光 从接近光检测部分，并且以这样的方式制作从基板的测量表面上的样品发射的荧光的测量，使得样品从激发光照射部分相对移动到光检测部分 用激发光。

    Pyrylium compound, process for making the same, nucleic acid stain, and labeled nucleic acid
    6.
    发明授权
    Pyrylium compound, process for making the same, nucleic acid stain, and labeled nucleic acid 有权
    吡喃鎓化合物，其制备方法，核酸染色和标记的核酸

    公开(公告)号：US06384237B1

    公开(公告)日：2002-05-07

    申请号：US09621668

    申请日：2000-07-24

    申请人： Tomohiro Suzuki ,  Tadashi Okamoto ,  Nobuko Yamamoto

    发明人： Tomohiro Suzuki ,  Tadashi Okamoto ,  Nobuko Yamamoto

    IPC分类号： C07D30934

    CPC分类号： C07D335/02 ,  C07D309/34

    摘要： A novel pyrylium compound is represented by the general formula (I): wherein X is oxygen or sulfur, Y− is a monovalent anion, n is an integer of 2 or 3, and M is hydrogen or an alkali metal. This pyrylium compound functions as a labeling agent by intermolecular bonding to nucleic acids, and as a fluorescence label having a chemical bond with the nucleic acids and an excitation wavelength in a visible light region.

    摘要翻译： 新颖的吡喃鎓化合物由通式（I）表示：其中X是氧或硫，Y-是一价阴离子，n是2或3的整数，M是氢或碱金属。 该吡喃鎓化合物通过与核酸的分子间键合作为标记剂，作为与核酸具有化学键和可见光区域的激发波长的荧光标记起作用。

    Method for analyzing base sequence of nucleic acid
    8.
    发明申请
    Method for analyzing base sequence of nucleic acid 审中-公开
    分析核酸碱基序列的方法

    公开(公告)号：US20050266403A9

    公开(公告)日：2005-12-01

    申请号：US10231302

    申请日：2002-08-30

    申请人： Nobuko Yamamoto ,  Tadashi Okamoto ,  Tomohiro Suzuki

    发明人： Nobuko Yamamoto ,  Tadashi Okamoto ,  Tomohiro Suzuki

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/6874 ,  C12Q1/6827 ,  C12Q1/6837 ,  G01N2500/00 ,  C12Q2565/513 ,  C12Q2563/107

    摘要： Provided is a method for performing a hybridization reaction that comprises the steps of providing a sample containing a target single-stranded nucleic acid and a probe array; heat-denaturing the probe array in a solution containing the sample; and reducing temperature to the extent suitable for a double-strand formation reaction, wherein the probe array remains immersed in the sample solution during reducing the temperature. Also disclosed is a method for detecting a certain sequence in a sample.

    摘要翻译： 提供了进行杂交反应的方法，其包括提供含有靶单链核酸和探针阵列的样品的步骤; 将探针阵列在含有样品的溶液中热变性; 并将温度降低至适合于双链形成反应的程度，其中探针阵列在降低温度期间保持浸入样品溶液中。 还公开了一种用于检测样品中某一序列的方法。

    Screening method for gene variation
    10.
    发明授权
    Screening method for gene variation 失效
    基因变异筛选方法

    公开(公告)号：US07445899B2

    公开(公告)日：2008-11-04

    申请号：US11119885

    申请日：2005-05-03

    申请人： Nobuko Yamamoto ,  Tadashi Okamoto ,  Shinya Tanaka ,  Tomohiro Suzuki

    发明人： Nobuko Yamamoto ,  Tadashi Okamoto ,  Shinya Tanaka ,  Tomohiro Suzuki

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6827 ,  B01J2219/00378 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00722 ,  C12Q1/6876 ,  C40B40/06 ,  C40B60/14 ,  C12Q2565/513 ,  C12Q2537/165

    摘要： A method for screening presence or absence of a variation in a region of a nucleic acid which comprises the steps of (a) preparing a sample containing a test nucleic acid corresponding to the region, (b) preparing a probe having a base sequence fully complementary to a normal sequence of the region, and a plurality of probes each having at least one base not complementary to the normal sequence, (c) fixing the probes in separate regions on a surface of a substrate to prepare a DNA array substrate, (d) reacting the test nucleic acid with the probes on the DNA array substrate, (e) measuring signals in each region where the signals are originated from respective hybrids formed between the test nucleic acid and one of the probes, and (f) calling variation in the test nucleic acid using a pattern of total signals of all regions.

    摘要翻译： 一种用于筛选核酸区域中存在或不存在变异的方法，其包括以下步骤：（a）制备含有对应于该区域的测试核酸的样品，（b）制备具有完全互补的碱基序列的探针 以及多个探针，其各自具有至少一个与正常序列不互补的碱基，（c）将探针固定在基底表面上的分离区域中以制备DNA阵列基底（d ）将测试核酸与DNA阵列底物上的探针反应，（e）测量每个区域中的信号，其中信号源自在测试核酸和探针之一之间形成的相应杂交体，以及（f）调用变异 测试核酸使用所有区域的总信号的模式。