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公开(公告)号：US06737238B2
公开(公告)日：2004-05-18
申请号：US09764419
申请日：2001-01-19
IPC分类号： C12Q168
CPC分类号： G01N33/54366 , B01J19/0046 , B01J2219/00326 , B01J2219/00536 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00659 , B01J2219/00689 , B01J2219/00707 , B01J2219/00722 , C40B40/06 , C40B60/14 , G01N35/00069 , G01N2035/00158 , Y10T436/110833 , Y10T436/143333
摘要： To provide a method of making measurements for a sample on the measuring surfaces of a substrate which makes it possible to simplify the control and construction of a measuring device, shorten the measuring period, make the measuring conditions constant, and improve the positional accuracy. The method and a device for carrying out the method are characterized in that measurements for the sample is performed by forming a circular orbit of detection areas, where detection is performed with a detector, on the measuring surfaces of the substrate while moving the detection areas relative to the substrate.
摘要翻译： 为了提供在基板的测量表面上对样品进行测量的方法，其使得可以简化测量装置的控制和构造，缩短测量周期，使测量条件恒定，并提高位置精度。 该方法和用于执行该方法的装置的特征在于，通过在基板的测量表面上形成检测区域的检测区域的圆形轨迹，同时使检测区域相对移动， 到基底。
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公开(公告)号：US06963397B2
公开(公告)日：2005-11-08
申请号：US09764049
申请日：2001-01-19
IPC分类号： G01J3/44 , G01N21/64 , G01N33/483
CPC分类号： G01N21/6458 , G01J3/4406 , G01N21/6428
摘要： To provide a method of efficiently removing excitation light in a device for measuring fluorescence emitted from samples on a measuring surface of a substrate while illuminating the samples with excitation light.The method is a fluorometry characterized in that an excitation light illumination portion where the samples are illuminated with the excitation light and a light detecting portion where measurements are made of the fluorescence are placed in such a manner as to make it possible to prevent the excitation light from approaching the light detecting portion, and measurements of the fluorescence emitted from the samples on the measuring surface of the substrate are made in such a manner as to relatively move the samples from the excitation light illumination portion to the light detecting portion after illuminating the same with the excitation light.
摘要翻译： 提供一种在用激发光照射样品的同时在基板的测量表面上测量从样品发射的荧光的装置中有效地去除激发光的方法。 该方法是一种荧光测定法，其特征在于，其中用激发光照射样品的激发光照射部分和其中测量荧光的光检测部分以使得可以防止激发光 从接近光检测部分，并且以这样的方式制作从基板的测量表面上的样品发射的荧光的测量，使得样品从激发光照射部分相对移动到光检测部分 用激发光。
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3.发明申请公开(公告)号：US20100267589A1
公开(公告)日：2010-10-21
申请号：US12723557
申请日：2010-03-12
IPC分类号： C40B50/14
CPC分类号： B01L3/0268 , B01J19/0046 , B01J2219/0036 , B01J2219/00378 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00722 , B41J2/14137 , B41J2002/1437 , B41J2002/14459 , C40B40/06 , C40B60/14
摘要： A liquid discharging device includes a plurality of liquid discharge sections. Each liquid discharge section includes a reservoir, a nozzle that discharges a solution supplied from the reservoir, and discharge energy generating means that generates energy to discharge the solution from the nozzle. The number of the liquid discharge sections corresponds to the number of probe types to be formed. The nozzles are two-dimensionally arranged. Using this liquid discharging device, probe liquids are discharged from the corresponding reservoirs onto a solid-phase substrate to form a predetermined two-dimensional probe array of high-purity probes on the substrate. This process exhibits high reproducibility and processability, and the resulting probe array has high array density.
摘要翻译： 液体排出装置包括多个液体排出部。 每个液体排出部分包括一个储存器，一个排出从储存器供应的溶液的喷嘴，以及产生排出能量的排出能量产生装置，以从喷嘴排出溶液。 液体排出部的数量对应于要形成的探针类型的数量。 喷嘴是二维排列的。 使用该液体排放装置，将探针液体从相应的储存器排出到固相基板上，以在基板上形成预定的高纯度探针的二维探针阵列。 该方法显示出高的再现性和可加工性，并且所得到的探针阵列具有高阵列密度。
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公开(公告)号：US07402384B2
公开(公告)日：2008-07-22
申请号：US10634510
申请日：2003-08-04
CPC分类号： B01J19/0046 , B01J2219/00274 , B01J2219/00378 , B01J2219/00432 , B01J2219/00497 , B01J2219/00527 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00675 , B01J2219/00722 , B01J2219/00725 , B01J2219/00729 , B82Y30/00 , G01N33/543
摘要： A probe array comprises a plurality of probes immobilized at a plurality of matrix sites on a substrate for capturing a target substance, wherein the probes are sequentially synthesized at the matrix sites on the substrate until a desired length, the probes are different from each other, and a labeling compound is coupled to each terminus of the probes in a final step of the synthesis. The probe array of the invention allows sensitive and reliable detection of the target substance. A method of evaluating the amount of the fully synthesized probes at respective matrix sites is also provided.
摘要翻译： 探针阵列包括固定在用于捕获靶物质的基底上的多个基质位点处的多个探针，其中探针在基底上的基质位点依次合成直到所需长度，探针彼此不同， 并且在合成的最后步骤中将标记化合物偶联到探针的每个末端。 本发明的探针阵列允许敏感且可靠地检测目标物质。 还提供了在各个基质位点评估完全合成的探针的量的方法。
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公开(公告)号：US20050266403A9
公开(公告)日：2005-12-01
申请号：US10231302
申请日：2002-08-30
CPC分类号： C12Q1/6874 , C12Q1/6827 , C12Q1/6837 , G01N2500/00 , C12Q2565/513 , C12Q2563/107
摘要： Provided is a method for performing a hybridization reaction that comprises the steps of providing a sample containing a target single-stranded nucleic acid and a probe array; heat-denaturing the probe array in a solution containing the sample; and reducing temperature to the extent suitable for a double-strand formation reaction, wherein the probe array remains immersed in the sample solution during reducing the temperature. Also disclosed is a method for detecting a certain sequence in a sample.
摘要翻译： 提供了进行杂交反应的方法，其包括提供含有靶单链核酸和探针阵列的样品的步骤; 将探针阵列在含有样品的溶液中热变性; 并将温度降低至适合于双链形成反应的程度，其中探针阵列在降低温度期间保持浸入样品溶液中。 还公开了一种用于检测样品中某一序列的方法。
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公开(公告)号：US20050202501A1
公开(公告)日：2005-09-15
申请号：US11119885
申请日：2005-05-03
IPC分类号： G01N31/22 , C07H21/04 , C12M1/00 , C12N15/09 , C12P19/34 , C12Q1/68 , C40B40/06 , C40B60/14 , G01N33/53 , G01N33/566 , G01N37/00 , G06F17/00
CPC分类号： C12Q1/6827 , B01J2219/00378 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00659 , B01J2219/00677 , B01J2219/00722 , C12Q1/6876 , C40B40/06 , C40B60/14 , C12Q2565/513 , C12Q2537/165
摘要： A method for screening presence or absence of a variation in a region of a nucleic acid which comprises the steps of (a) preparing a sample containing a test nucleic acid corresponding to the region, (b) preparing a probe having a base sequence fully complementary to a normal sequence of the region, and a plurality of probes each having at least one base not complementary to the normal sequence, (c) fixing the probes in separate regions on a surface of a substrate to prepare a DNA array substrate, (d) reacting the test nucleic acid with the probes on the DNA array substrate, (e) measuring signals in each region where the signals are originated from respective hybrids formed between the test nucleic acid and one of the probes, and (f) calling variation in the test nucleic acid using a pattern of total signals of all regions.
摘要翻译： 一种用于筛选核酸区域中存在或不存在变异的方法，其包括以下步骤：（a）制备含有对应于该区域的测试核酸的样品，（b）制备具有完全互补的碱基序列的探针 以及多个探针，其各自具有至少一个与正常序列不互补的碱基，（c）将探针固定在基底表面上的分离区域中以制备DNA阵列基底（d ）将测试核酸与DNA阵列底物上的探针反应，（e）测量每个区域中的信号，其中信号源自在测试核酸和探针之一之间形成的相应杂交体，以及（f）调用变异 测试核酸使用所有区域的总信号的模式。
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公开(公告)号：US20080051293A1
公开(公告)日：2008-02-28
申请号：US11896141
申请日：2007-08-30
IPC分类号： C40B20/02
CPC分类号： C12Q1/6874 , C12Q2565/519
摘要： A method for identifying an unknown base sequence present in a target single-stranded nucleic acid utilizing a probe array in which single-stranded nucleic acid probes are arranged as isolated spots on a substrate, where each probe has a base sequence complementary to one of the plural base sequences expected to be the unknown base sequence, and a fluorescence pattern of a sample on the probe array is compared with template patterns to identify the base sequence of the sample.
摘要翻译： 一种利用探针阵列鉴定目标单链核酸中存在的未知碱基序列的方法，其中单链核酸探针作为分离斑点排列在底物上，其中每个探针具有与 将预期为未知碱基序列的多个碱基序列和探针阵列上的样品的荧光图案与模板图案进行比较以鉴定样品的碱基序列。
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公开(公告)号：US07273697B2
公开(公告)日：2007-09-25
申请号：US09942596
申请日：2001-08-31
CPC分类号： C12Q1/6874 , C12Q2565/519
摘要： A method for identifying an unknown base sequence present in a target single-stranded nucleic acid utilizing a probe array in which single-stranded nucleic acid probes are arranged as isolated spots on a substrate, where each probe has a base sequence complementary to one of the plural base sequences expected to be the unknown base sequence, and a fluorescence pattern of a sample on the probe array is compared with template patterns to identify the base sequence of the sample.
摘要翻译： 一种利用探针阵列鉴定目标单链核酸中存在的未知碱基序列的方法，其中单链核酸探针作为分离斑点排列在底物上，其中每个探针具有与 将预期为未知碱基序列的多个碱基序列和探针阵列上的样品的荧光图案与模板图案进行比较以鉴定样品的碱基序列。
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9.发明授权公开(公告)号：US06960432B2
公开(公告)日：2005-11-01
申请号：US09764050
申请日：2001-01-19
CPC分类号： C12Q1/6813 , C12Q2563/107
摘要： A method for dry detection/quantification of targeted nucleotide chains, comprising the steps of:(1) realizing a state in which a hybrid (C) of a certain amount of targeted nucleotide chain (A), which is derived from a sample solution and subjected to detection or quantification, and a probe nucleotide chain (B), which has a base sequence complementary to a specific site of the base sequence of the targeted nucleotide chain, is formed on a solid-phase substrate by mutually reacting the two types of nucleotide chains with each other, and in which there exists a fluorescence dye (D), which acts on the hybrid (C), thereby emits fluorescence or increases its fluorescence intensity, and is capable of continuing to emit fluorescence even in the dried state while acting on the hybrid;(2) drying the hybrid (C) and the fluorescence dye (D) on the substrate; and(3) measuring the fluorescence emitted from the fluorescence dye (D), as a measuring means, after the drying operation.
摘要翻译： 一种靶向核苷酸链的干检测/定量方法，包括以下步骤：（1）实现从样品溶液衍生的一定量的靶向核苷酸链（A）的杂合体（C）和 进行检测或定量，并且在固相基板上形成具有与目标核苷酸链的碱基序列的特定位点互补的碱基序列的探针核苷酸链（B），通过使两种类型的 核苷酸链彼此存在，其中存在作用于杂化物（C）的荧光染料（D），从而发射荧光或增加其荧光强度，并且即使在干燥状态下也能够继续发射荧光，而 作用于混合动力; （2）在基材上干燥杂化物（C）和荧光染料（D）; 和（3）在干燥操作之后测量从荧光染料（D）发出的荧光作为测量装置。
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10.发明授权Reaction site array with a black matrix to decrease cross-contamination between reaction regions 失效具有黑色矩阵的反应位点阵列以减少反应区域之间的交叉污染公开(公告)号：US06548020B2
公开(公告)日：2003-04-15
申请号：US09126856
申请日：1998-07-31
IPC分类号： G01N1500
CPC分类号： G01N33/545 , B01J19/0046 , B01J2219/00317 , B01J2219/00378 , B01J2219/00527 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00621 , B01J2219/00637 , B01J2219/00659 , B01J2219/00722 , B01J2219/00729 , C40B40/06 , C40B60/14 , G01N33/543 , G01N33/54366
摘要： A reaction site array to be used in conducting a plurality of reactions between two or more kinds of substances in a liquid medium with a trace amount is provided. The reaction site array comprises a plurality of reaction sites separated each other, each of the reaction sites is composed of a first region and a second region, the second region is raised from the first region to separate the first regions each other, and the first region has an affinity to the liquid medium and the second region has an affinity to the liquid medium lower than that of the first region. A preparation process of the reaction site array and a reaction process using the reaction site array are also provided.
摘要翻译： 提供了用于在微量液体介质中的两种或更多种物质之间进行多次反应的反应位点阵列。 反应位点阵列包括彼此分离的多个反应位点，每个反应位点由第一区域和第二区域组成，第二区域从第一区域升高以将第一区域彼此分开，第一区域 区域对液体介质具有亲和性，第二区域对液体介质的亲和性低于第一区域的亲和性。 还提供了反应位点阵列的制备方法和使用反应位点阵列的反应方法。
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