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    Fluorometry and fluorometric device and substrate suitable therefor
    2.
    发明授权
    Fluorometry and fluorometric device and substrate suitable therefor 失效
    荧光测定法和荧光测定装置及适用的底物

    公开(公告)号：US06963397B2

    公开(公告)日：2005-11-08

    申请号：US09764049

    申请日：2001-01-19

    申请人： Tomohiro Suzuki ,  Tadashi Okamoto ,  Kazuhiro Matsumoto ,  Nobuko Yamamoto

    发明人： Tomohiro Suzuki ,  Tadashi Okamoto ,  Kazuhiro Matsumoto ,  Nobuko Yamamoto

    IPC分类号： G01J3/44 ,  G01N21/64 ,  G01N33/483

    CPC分类号： G01N21/6458 ,  G01J3/4406 ,  G01N21/6428

    摘要： To provide a method of efficiently removing excitation light in a device for measuring fluorescence emitted from samples on a measuring surface of a substrate while illuminating the samples with excitation light.The method is a fluorometry characterized in that an excitation light illumination portion where the samples are illuminated with the excitation light and a light detecting portion where measurements are made of the fluorescence are placed in such a manner as to make it possible to prevent the excitation light from approaching the light detecting portion, and measurements of the fluorescence emitted from the samples on the measuring surface of the substrate are made in such a manner as to relatively move the samples from the excitation light illumination portion to the light detecting portion after illuminating the same with the excitation light.

    摘要翻译： 提供一种在用激发光照射样品的同时在基板的测量表面上测量从样品发射的荧光的装置中有效地去除激发光的方法。 该方法是一种荧光测定法，其特征在于，其中用激发光照射样品的激发光照射部分和其中测量荧光的光检测部分以使得可以防止激发光 从接近光检测部分，并且以这样的方式制作从基板的测量表面上的样品发射的荧光的测量，使得样品从激发光照射部分相对移动到光检测部分 用激发光。

    Pyrylium compound, process for making the same, nucleic acid stain, and labeled nucleic acid
    3.
    发明授权
    Pyrylium compound, process for making the same, nucleic acid stain, and labeled nucleic acid 有权
    吡喃鎓化合物，其制备方法，核酸染色和标记的核酸

    公开(公告)号：US06384237B1

    公开(公告)日：2002-05-07

    申请号：US09621668

    申请日：2000-07-24

    申请人： Tomohiro Suzuki ,  Tadashi Okamoto ,  Nobuko Yamamoto

    发明人： Tomohiro Suzuki ,  Tadashi Okamoto ,  Nobuko Yamamoto

    IPC分类号： C07D30934

    CPC分类号： C07D335/02 ,  C07D309/34

    摘要： A novel pyrylium compound is represented by the general formula (I): wherein X is oxygen or sulfur, Y− is a monovalent anion, n is an integer of 2 or 3, and M is hydrogen or an alkali metal. This pyrylium compound functions as a labeling agent by intermolecular bonding to nucleic acids, and as a fluorescence label having a chemical bond with the nucleic acids and an excitation wavelength in a visible light region.

    摘要翻译： 新颖的吡喃鎓化合物由通式（I）表示：其中X是氧或硫，Y-是一价阴离子，n是2或3的整数，M是氢或碱金属。 该吡喃鎓化合物通过与核酸的分子间键合作为标记剂，作为与核酸具有化学键和可见光区域的激发波长的荧光标记起作用。

    Method for analyzing base sequence of nucleic acid
    6.
    发明申请
    Method for analyzing base sequence of nucleic acid 审中-公开
    分析核酸碱基序列的方法

    公开(公告)号：US20050266403A9

    公开(公告)日：2005-12-01

    申请号：US10231302

    申请日：2002-08-30

    申请人： Nobuko Yamamoto ,  Tadashi Okamoto ,  Tomohiro Suzuki

    发明人： Nobuko Yamamoto ,  Tadashi Okamoto ,  Tomohiro Suzuki

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/6874 ,  C12Q1/6827 ,  C12Q1/6837 ,  G01N2500/00 ,  C12Q2565/513 ,  C12Q2563/107

    摘要： Provided is a method for performing a hybridization reaction that comprises the steps of providing a sample containing a target single-stranded nucleic acid and a probe array; heat-denaturing the probe array in a solution containing the sample; and reducing temperature to the extent suitable for a double-strand formation reaction, wherein the probe array remains immersed in the sample solution during reducing the temperature. Also disclosed is a method for detecting a certain sequence in a sample.

    摘要翻译： 提供了进行杂交反应的方法，其包括提供含有靶单链核酸和探针阵列的样品的步骤; 将探针阵列在含有样品的溶液中热变性; 并将温度降低至适合于双链形成反应的程度，其中探针阵列在降低温度期间保持浸入样品溶液中。 还公开了一种用于检测样品中某一序列的方法。

    Method for analyzing base sequence of nucleic acid
    7.
    发明申请
    Method for analyzing base sequence of nucleic acid 审中-公开
    分析核酸碱基序列的方法

    公开(公告)号：US20080051293A1

    公开(公告)日：2008-02-28

    申请号：US11896141

    申请日：2007-08-30

    申请人： Nobuko Yamamoto ,  Tadashi Okamoto ,  Tomohiro Suzuki

    发明人： Nobuko Yamamoto ,  Tadashi Okamoto ,  Tomohiro Suzuki

    IPC分类号： C40B20/02

    CPC分类号： C12Q1/6874 ,  C12Q2565/519

    摘要： A method for identifying an unknown base sequence present in a target single-stranded nucleic acid utilizing a probe array in which single-stranded nucleic acid probes are arranged as isolated spots on a substrate, where each probe has a base sequence complementary to one of the plural base sequences expected to be the unknown base sequence, and a fluorescence pattern of a sample on the probe array is compared with template patterns to identify the base sequence of the sample.

    摘要翻译： 一种利用探针阵列鉴定目标单链核酸中存在的未知碱基序列的方法，其中单链核酸探针作为分离斑点排列在底物上，其中每个探针具有与 将预期为未知碱基序列的多个碱基序列和探针阵列上的样品的荧光图案与模板图案进行比较以鉴定样品的碱基序列。

    Method for analyzing base sequence of nucleic acid
    8.
    发明授权
    Method for analyzing base sequence of nucleic acid 失效
    分析核酸碱基序列的方法

    公开(公告)号：US07273697B2

    公开(公告)日：2007-09-25

    申请号：US09942596

    申请日：2001-08-31

    申请人： Nobuko Yamamoto ,  Tadashi Okamoto ,  Tomohiro Suzuki

    发明人： Nobuko Yamamoto ,  Tadashi Okamoto ,  Tomohiro Suzuki

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6874 ,  C12Q2565/519

    摘要： A method for identifying an unknown base sequence present in a target single-stranded nucleic acid utilizing a probe array in which single-stranded nucleic acid probes are arranged as isolated spots on a substrate, where each probe has a base sequence complementary to one of the plural base sequences expected to be the unknown base sequence, and a fluorescence pattern of a sample on the probe array is compared with template patterns to identify the base sequence of the sample.

    摘要翻译： 一种利用探针阵列鉴定目标单链核酸中存在的未知碱基序列的方法，其中单链核酸探针作为分离斑点排列在底物上，其中每个探针具有与 将预期为未知碱基序列的多个碱基序列和探针阵列上的样品的荧光图案与模板图案进行比较以鉴定样品的碱基序列。

    Detection/quantification of targeted nucleotide chains, and detection/quantification of multi-stranded nucleotide chains by fluorescence
    9.
    发明授权
    Detection/quantification of targeted nucleotide chains, and detection/quantification of multi-stranded nucleotide chains by fluorescence 失效
    靶核苷酸链的检测/定量，以及通过荧光检测/定量多链核苷酸链

    公开(公告)号：US06960432B2

    公开(公告)日：2005-11-01

    申请号：US09764050

    申请日：2001-01-19

    申请人： Tadashi Okamoto ,  Nobuko Yamamoto ,  Tomohiro Suzuki

    发明人： Tadashi Okamoto ,  Nobuko Yamamoto ,  Tomohiro Suzuki

    IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04 ,  C12P19/34

    CPC分类号： C12Q1/6813 ,  C12Q2563/107

    摘要： A method for dry detection/quantification of targeted nucleotide chains, comprising the steps of:(1) realizing a state in which a hybrid (C) of a certain amount of targeted nucleotide chain (A), which is derived from a sample solution and subjected to detection or quantification, and a probe nucleotide chain (B), which has a base sequence complementary to a specific site of the base sequence of the targeted nucleotide chain, is formed on a solid-phase substrate by mutually reacting the two types of nucleotide chains with each other, and in which there exists a fluorescence dye (D), which acts on the hybrid (C), thereby emits fluorescence or increases its fluorescence intensity, and is capable of continuing to emit fluorescence even in the dried state while acting on the hybrid;(2) drying the hybrid (C) and the fluorescence dye (D) on the substrate; and(3) measuring the fluorescence emitted from the fluorescence dye (D), as a measuring means, after the drying operation.

    摘要翻译： 一种靶向核苷酸链的干检测/定量方法，包括以下步骤：（1）实现从样品溶液衍生的一定量的靶向核苷酸链（A）的杂合体（C）和 进行检测或定量，并且在固相基板上形成具有与目标核苷酸链的碱基序列的特定位点互补的碱基序列的探针核苷酸链（B），通过使两种类型的 核苷酸链彼此存在，其中存在作用于杂化物（C）的荧光染料（D），从而发射荧光或增加其荧光强度，并且即使在干燥状态下也能够继续发射荧光，而 作用于混合动力; （2）在基材上干燥杂化物（C）和荧光染料（D）; 和（3）在干燥操作之后测量从荧光染料（D）发出的荧光作为测量装置。