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    Polynucleotides encoding SREB1 receptor
    5.
    发明授权
    Polynucleotides encoding SREB1 receptor 有权
    编码SREB1受体的多核苷酸

    公开(公告)号：US07341849B2

    公开(公告)日：2008-03-11

    申请号：US10898329

    申请日：2004-07-26

    申请人： Mitsuyuki Matsumoto ,  Toru Sugimoto ,  Jun Takasaki ,  Masazumi Kamohara ,  Tetsu Saito ,  Masato Kobayashi

    发明人： Mitsuyuki Matsumoto ,  Toru Sugimoto ,  Jun Takasaki ,  Masazumi Kamohara ,  Tetsu Saito ,  Masato Kobayashi

    IPC分类号： C12P23/00 ,  C12N15/00 ,  C12N5/00 ,  C12N1/20 ,  C12Q1/16

    CPC分类号： C07K16/28 ,  A61K38/00 ,  C07K14/705 ,  C07K14/723

    摘要： This invention belongs to the genetic engineering field, and provides novel G protein-coupled receptor family proteins SREB1, SREB2 and SREB3 expressed in the central nervous system, genes coding for these proteins, screening methods using these proteins and so on. As one of the methods for obtaining the G protein-coupled receptor proteins of the present invention, RT-PCR is carried out using mRNA extracted from human or rat brain tissue or brain-derived cells as the template and using two primers interposing the entire portion or a part of the G protein-coupled receptor protein translation region, thereby obtaining cDNA corresponding to the G protein-coupled receptor protein or a part thereof, and the cDNA is integrated into an appropriate expression vector and expressed in a host cell.

    摘要翻译： 本发明属于遗传工程领域，提供了在中枢神经系统中表达的新型G蛋白偶联受体家族蛋白SREB1，SREB2和SREB3，编码这些蛋白质的基因，使用这些蛋白质的筛选方法等。 作为获得本发明的G蛋白偶联受体蛋白质的方法之一，使用从人或大鼠脑组织或脑源性细胞提取的mRNA作为模板，使用插入整个部分的两个引物进行RT-PCR 或G蛋白偶联受体蛋白质翻译区的一部分，从而获得对应于G蛋白偶联受体蛋白或其一部分的cDNA，并将cDNA整合到合适的表达载体中并在宿主细胞中表达。

    Method for screening agents for the treatment of diabetes
    7.
    发明授权
    Method for screening agents for the treatment of diabetes 有权
    用于治疗糖尿病的筛选剂的方法

    公开(公告)号：US07662775B2

    公开(公告)日：2010-02-16

    申请号：US10975367

    申请日：2004-10-29

    申请人： Takahide Ohishi ,  Jun Takasaki ,  Mitsuyuki Matsumoto ,  Tetsu Saito ,  Masazumi Kamohara ,  Takatoshi Soga

    发明人： Takahide Ohishi ,  Jun Takasaki ,  Mitsuyuki Matsumoto ,  Tetsu Saito ,  Masazumi Kamohara ,  Takatoshi Soga

    IPC分类号： A61K38/00 ,  A61K51/00 ,  C07K1/00 ,  C07K14/00

    CPC分类号： C07K14/705 ,  A61K38/00 ,  C07K14/723

    摘要： The invention relates to a convenient screening tool for identifying an agent for treating diabetes. The screening tool is a G protein-coupled receptor, a variant of the G protein-coupled receptor, or a homolog of the G protein-coupled receptor, which promotes insulin secretion under a high glucose concentration by activation. The invention also relates to cells transformed with an expression vector comprising a polynucleotide encoding and expressing the screening tool. The invention provides a convenient screening method for identifying an agent for treating diabetes, pharmaceutical compositions comprising the agent, and a process for manufacturing the pharmaceutical composition.

    摘要翻译： 本发明涉及用于鉴定用于治疗糖尿病的药剂的方便的筛选工具。 筛选工具是G蛋白偶联受体，G蛋白偶联受体的变体或G蛋白偶联受体的同系物，其通过活化促进高葡萄糖浓度下的胰岛素分泌。 本发明还涉及用包含编码和表达筛选工具的多核苷酸的表达载体转化的细胞。 本发明提供了用于鉴定用于治疗糖尿病的药剂的方便的筛选方法，包含该药剂的药物组合物和用于制备该药物组合物的方法。

    Therapeutic Medicament for Chronic Obstructive Pulmonary Disease (COPD), Cystic Fibrosis, and Pulmonary Hypertension
    8.
    发明申请
    Therapeutic Medicament for Chronic Obstructive Pulmonary Disease (COPD), Cystic Fibrosis, and Pulmonary Hypertension 审中-公开
    慢性阻塞性肺疾病（COPD），囊性纤维化和肺动脉高压治疗药物

    公开(公告)号：US20090215868A1

    公开(公告)日：2009-08-27

    申请号：US11794167

    申请日：2006-01-12

    申请人： Kunimiki Otsu ,  Tetsu Saito ,  Takefumi Gemba

    发明人： Kunimiki Otsu ,  Tetsu Saito ,  Takefumi Gemba

    IPC分类号： A61K31/711 ,  C07H21/04 ,  A61K48/00

    CPC分类号： A61K9/0073 ,  A61K9/0075 ,  A61K9/0078 ,  A61K9/008 ,  A61K31/7088 ,  A61K31/713 ,  C12N15/113 ,  C12N2310/13

    摘要： The present invention provides a prophylactic, therapeutic or ameliorative medicament for chronic obstructive pulmonary disease (COPD), cystic fibrosis or pulmonary hypertension. More specifically, the present invention provides: (1) a prophylactic, therapeutic or ameliorative medicament for chronic obstructive pulmonary disorder (COPD), cystic fibrosis or pulmonary hypertension, having an NF-κB decoy as the active ingredient thereof; (2) the NF-κB decoy is an oligonucleotide containing the binding sequence GGGRHTYYC (where R represents either A or G; Y represents either C or T; and H represents either A, C or T); (3) an oligonucleotide wherein the NF-κB binding sequence is GGGATTTCCC or GGGACTTTCC; (4) an oligonucleotide wherein the NF-κB decoy is represented by SEQ ID NO:3; (5) the NF-κB decoy is a double-stranded oligonucleotide; (6) the NF-κB decoy is administered in the form of a fine powder; and (7) the NF-κB decoy fine powder is a dry powder.

    摘要翻译： 本发明提供了用于慢性阻塞性肺疾病（COPD），囊性纤维化或肺动脉高压的预防，治疗或改善药物。 更具体地，本发明提供：（1）具有NF-κB诱饵作为其活性成分的用于慢性阻塞性肺病（COPD），囊性纤维化或肺动脉高压的预防，治疗或改善药物; （2）NF-κB诱饵是含有结合序列GGGRHTYYC（其中R表示A或G; Y表示C或T; H表示A，C或T）的寡核苷酸; （3）NF-κB结合序列为GGGATTTCCC或GGGACTTTCC的寡核苷酸; （4）其中NF-κB诱饵由SEQ ID NO：3表示的寡核苷酸; （5）NF-κB诱饵是双链寡核苷酸; （6）NF-κB诱饵以细粉末的形式施用; 和（7）NF-κB诱饵细粉是干粉。

    Lipopolysaccharide binding protein and process for producing the same
    9.
    发明授权
    Lipopolysaccharide binding protein and process for producing the same 失效
    脂多糖结合蛋白及其制备方法

    公开(公告)号：US5760177A

    公开(公告)日：1998-06-02

    申请号：US393058

    申请日：1995-02-23

    申请人： Sadaaki Iwanaga ,  Shunichiro Kawabata ,  Tetsu Saito

    发明人： Sadaaki Iwanaga ,  Shunichiro Kawabata ,  Tetsu Saito

    IPC分类号： A61K9/08 ,  A61K38/00 ,  C07K1/22 ,  C07K14/00 ,  C07K14/435 ,  G01N33/579 ,  G01N33/92 ,  A61K39/385

    CPC分类号： C07K14/43509 ,  A61K38/00 ,  Y10S530/82

    摘要： The invention provides a lipopolysaccharide (LPS) binding protein isolated from horseshoe crab. The LPS binding protein is isolated by (i) extracting the hemocyte membrane fraction of horseshoe crab with a polyethylene glycol ether type nonionic surface active agent in the presence of Ca ions, (ii) combining the extract with immobilized LPS under conditions that permit the LPS binding protein to bind the immobilized LPS to produce an LPS-LPS binding protein complex, and (iii) harvesting the LPS binding protein released from the complex in the presence of a chelating agent. The isolated LPS binding protein has a molecular weight of about 27,000 daltons as determined by SDS polyacrylamide gel electrophoresis and is operative to bind a lipopolysaccharide endotoxin. Accordingly, the isolated LPS binding protein can be used for detecting endotoxin and/or removing endotoxin from an injectable medicine.

    摘要翻译： 本发明提供从马蹄蟹分离的脂多糖（LPS）结合蛋白。 通过（i）在Ca离子存在下用聚乙二醇醚型非离子表面活性剂提取鲎的血细胞膜级分，分离LPS结合蛋白，（ii）在允许LPS的条件下将提取物与固定的LPS组合 结合蛋白质以结合固定的LPS以产生LPS-LPS结合蛋白复合物，和（iii）在螯合剂存在下从复合物中收获释放的LPS结合蛋白。 通过SDS聚丙烯酰胺凝胶电泳测定，分离的LPS结合蛋白具有约27,000道尔顿的分子量，并且可操作地结合脂多糖内毒素。 因此，分离的LPS结合蛋白可用于从可注射药物中检测内毒素和/或除去内毒素。