公开(公告)号：US20220146511A1

公开(公告)日：2022-05-12

申请号：US17349169

申请日：2021-06-16

申请人： The Regents of the University of California

发明人： M. Kursad Araz ,  Akwasi A. Apori ,  Amy E. Herr

IPC分类号： G01N33/559 ,  B01L3/00 ,  G01N33/558 ,  G01N33/543 ,  G01N27/447

摘要： A microfluidic device for determining whether an analyte is present in a sample is provided. The microfluidic device includes an elongated flow path having a polymeric medium, where the polymeric medium includes a first analyte detection domain having a first immobilized capture member that specifically binds to a first analyte and a second analyte detection domain having a second immobilized capture member that specifically binds to a second analyte. Also provided are methods, systems and kits in which the subject microfluidic devices find use, as well as methods of producing the same.

公开(公告)号：US09671368B2

公开(公告)日：2017-06-06

申请号：US14274409

申请日：2014-05-09

申请人： The Regents of the University of California

发明人： Amy E. Herr ,  Augusto Tentori

IPC分类号： G01N27/453 ,  G01N27/447

CPC分类号： G01N27/44795 ,  G01N27/44773 ,  G01N27/44791

摘要： Microfluidic devices and methods for using the same are provided. Embodiments include microfluidic devices that have a first separation region configured to separate a sample along a first directional axis based on a first property, and a second separation region in fluid communication with the first separation region and configured to separate the sample along a second directional axis based on a second property. Also provided are methods of using the devices as well as systems and kits that include the devices. The devices, systems and methods find use in a variety of different applications, including diagnostic and validation assays.

公开(公告)号：US20160011190A1

公开(公告)日：2016-01-14

申请号：US14798103

申请日：2015-07-13

申请人： The Regents of the University of California

发明人： Amy E. Herr ,  Alex James Hughes

IPC分类号： G01N33/559 ,  B01L3/00

CPC分类号： G01N33/559 ,  B01L3/5023 ,  B01L3/5027 ,  B01L3/502753 ,  B01L2200/10 ,  B01L2300/069 ,  B01L2300/0816 ,  B01L2300/1861 ,  B01L2400/0418 ,  B01L2400/0496 ,  G01N27/44756 ,  G01N33/54366 ,  G01N33/561

摘要： Microfluidic devices and methods for using the same are provided. Aspects of the present disclosure include microfluidic devices that include a separation medium having functional groups which covalently bond to one or more analytes of interest, e.g., proteins, in a sample upon application of an applied stimulus, e.g., light. Also provided are methods of using the devices as well as systems and kits that include the devices. The devices, systems and methods find use in a variety of different applications, including diagnostic and validation assays.

摘要翻译： 提供微流体装置及其使用方法。 本公开的方面包括微流体装置，其包括具有在应用施加的刺激例如光时在样品中共价键合到一种或多种感兴趣的分析物（例如蛋白质）的官能团的分离介质。 还提供了使用设备的方法以及包括设备的系统和套件。 设备，系统和方法可用于各种不同的应用，包括诊断和验证测定。

公开(公告)号：US12038407B2

公开(公告)日：2024-07-16

申请号：US17327806

申请日：2021-05-24

申请人： The Regents of the University of California

发明人： Amy E. Herr ,  Lin He ,  Andrew J. Modzelewski ,  Elisabet Rosàs-Canyelles

IPC分类号： G01N27/447 ,  C08J9/40 ,  C08K5/1545 ,  C08L33/26

CPC分类号： G01N27/44747 ,  C08J9/40 ,  C08K5/1545 ,  C08L33/26 ,  C08J2333/26

摘要： Dual nucleic acid and protein isoform measurements are performed on low starting cell numbers (e.g. equivalent to the number of blastomeres composing early embryonic development stages (morula and blastocysts)), comprising integrating fractionation polyacrylamide gel electrophoresis (fPAGE) of 10-100 cells with off-chip analysis of nucleic acids in the nuclei.

公开(公告)号：US10393701B2

公开(公告)日：2019-08-27

申请号：US14035846

申请日：2013-09-24

申请人： The Regents of the University of California

发明人： Amy E. Herr ,  Augusto M. Tentori ,  Alex J. Hughes

IPC分类号： G01N27/447 ,  G01N30/00 ,  G01N30/88

摘要： Microfluidic methods of assaying molecule switching are provided. Aspects of the methods include microfluidically separating a sample containing the molecule of interest and then employing the resultant separation pattern to determine a switching characteristic of the molecule. Also provided are microfluidic devices, as well as systems and kits that include the devices, which find use in practicing embodiments of the methods. The methods, devices, systems and kits find use in a variety of different applications, such as analytical and diagnostic assays.

公开(公告)号：US10267795B2

公开(公告)日：2019-04-23

申请号：US14650520

申请日：2014-03-06

申请人： The Regents of the University of California

发明人： Amy E. Herr ,  Alex James Hughes ,  Elly Sinkala ,  Todd A. Duncombe ,  Kevin A. Yamauchi ,  Julea Vlassakis ,  Chi-Chih Kang ,  Zhuchen Xu ,  Robert Lin

IPC分类号： G01N27/447 ,  G01N27/453 ,  G01N33/561

摘要： Electrophoretic separation devices and methods for using the same are provided. Aspects of the devices include a polymeric separation medium that includes a plurality of microwells. Also provided are methods, systems and kits in which the subject devices find use. The devices and methods find use in a variety of different electrophoretic separation applications.

公开(公告)号：US20170242020A1

公开(公告)日：2017-08-24

申请号：US15310065

申请日：2015-05-29

申请人： The Regents of the University of California

发明人： Kevin A. Yamauchi ,  Amy E. Herr

IPC分类号： G01N33/68 ,  G01N27/447 ,  G01N33/543 ,  G01N33/545 ,  G01N33/561

CPC分类号： G01N33/6803 ,  C07K1/26 ,  C12N15/101 ,  C12Q2565/125 ,  G01N27/44747 ,  G01N27/44756 ,  G01N27/44791 ,  G01N33/54306 ,  G01N33/545 ,  G01N33/561 ,  G01N2550/00 ,  G01N2570/00

摘要： Electrophoretic separation methods and systems for performing the same are provided. The methods and systems find use in a variety of different electrophoretic separation applications, such as sub-cellular Western blotting of single cells.

公开(公告)号：US20150293089A1

公开(公告)日：2015-10-15

申请号：US14440852

申请日：2013-11-11

申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

发明人： M. Kursad Araz ,  Akwasi A. Apori ,  Amy E. Herr

IPC分类号： G01N33/559 ,  G01N27/447

CPC分类号： G01N33/559 ,  B01L3/5027 ,  B01L3/502707 ,  B01L2300/0636 ,  B01L2300/0825 ,  B01L2300/087 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0487 ,  G01N27/447 ,  G01N33/54366 ,  G01N33/558 ,  G01N2333/186

摘要： A microfluidic device for determining whether an analyte is present in a sample is provided. The microfluidic device includes an elongated flow path having a polymeric medium, where the polymeric medium includes a first analyte detection domain having a first immobilized capture member that specifically binds to a first analyte and a second analyte detection domain having a second immobilized capture member that specifically binds to a second analyte. Also provided are methods, systems and kits in which the subject microfluidic devices find use, as well as methods of producing the same.

摘要翻译： 提供用于确定样品中是否存在分析物的微流体装置。 所述微流体装置包括具有聚合物介质的细长流动路径，其中所述聚合物介质包括第一分析物检测结构域，所述第一分析物检测结构域具有特异性结合第一分析物的第一固定捕获构件和具有第二固定化捕获元件的第二分析物检测区域， 结合第二分析物。 还提供了本发明的微流体装置用于其的方法，系统和试剂盒，以及其制备方法。

公开(公告)号：US20150001080A1

公开(公告)日：2015-01-01

申请号：US14372969

申请日：2013-02-01

申请人： The Regents of the University of California

发明人： Amy E. Herr ,  Todd Duncombe

IPC分类号： G01N27/447

CPC分类号： G01N27/44756 ,  G01N27/44717 ,  G01N27/44791

摘要： Embodiments of an electrophoresis device and methods for using the same in analyte separation applications are provided. Certain embodiments of the present disclosure include microfluidic devices having a single electrophoretic separation channel containing a separation medium. Systems according to embodiments of the present disclosure are configured to monitor analyte fronts moving through the electrophoretic separation channel in order to detect differentially migrating analytes, i.e., the systems are configured for moving boundary electrophoresis (MBE).

摘要翻译： 提供了电泳装置的实施例及其在分析物分离应用中的使用方法。 本公开的某些实施方案包括具有包含分离介质的单个电泳分离通道的微流体装置。 根据本公开实施例的系统被配置为监测移动通过电泳分离通道的分析物前端，以便检测差异迁移的分析物，即系统被配置用于移动边界电泳（MBE）。

公开(公告)号：US20180217094A1

公开(公告)日：2018-08-02

申请号：US15749039

申请日：2016-09-08

申请人： The Regents of the University of California

发明人： Amy E. Herr ,  Augusto M. Tentori ,  Kevin A. Yamauchi

IPC分类号： G01N27/447 ,  B01D57/02

CPC分类号： G01N27/44747 ,  B01D57/02 ,  G01N27/44791 ,  G01N27/44795

摘要： Isoelectric focusing devices configured for multiplex separation of sample components of interest in a polymeric separation medium are provided. Also provided are methods of using the devices as well as systems and kits that include the devices. The devices, systems and methods find use in a variety of different applications, including diagnostic and validation assays.