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11 条记录 (耗时 0.033 秒)

    Three-dimensional cell growth assay
    1.
    发明授权
    Three-dimensional cell growth assay 失效
    三维细胞生长测定

    公开(公告)号：US06602701B2

    公开(公告)日：2003-08-05

    申请号：US09758644

    申请日：2001-01-11

    申请人： Thomas S. Deisboeck ,  E. Antonio Chiocca ,  John D. Wright

    发明人： Thomas S. Deisboeck ,  E. Antonio Chiocca ,  John D. Wright

    IPC分类号： C12M300

    CPC分类号： C12M41/36 ,  C12M23/34

    摘要： The invention relates to devices and methods for growing cells in vitro in an enclosed device that allows for a three-dimensional measurement over time of both their proliferative and/or invasive properties. By growing the cells in an enclosed matrix that resembles the environment that the cells confront in vivo, the cells can divide, invade, and form branched networks as they do in living tissue, e.g., in an individual. The devices of the invention include a test chamber in which cells, e.g., tumor cells, are placed and permitted to divide and/or invade. Cells can be placed within an insert within a chamber of the device. A delivery chamber that connects to the test chamber enables the delivery of agents that can be studied, e.g., for their therapeutic potential. The assay devices of the invention can be used as model systems to study cancer biology and to evaluate the efficacy of anti-cancer therapeutics.

    摘要翻译： 本发明涉及用于在封闭装置中体外培养细胞的装置和方法，所述装置和方法允许随着时间的推移进行三维测量，包括它们的增殖和/或侵入性质。 通过将细胞生长在类似于细胞在体内所面临的环境的封闭的基质中，细胞可如在活组织中例如在个体中分开，侵入并形成分支的网络。 本发明的装置包括测试室，其中放置例如肿瘤细胞的细胞并允许分裂和/或侵入细胞。 细胞可以放置在装置的腔室内的插入物内。 连接到测试室的输送室能够输送可以研究的药剂，例如用于其治疗潜力。 本发明的测定装置可用作模型系统来研究癌症生物学并评估抗癌治疗剂的功效。

    Three-dimensional cell growth assay
    2.
    发明授权
    Three-dimensional cell growth assay 失效
    三维细胞生长测定

    公开(公告)号：US06849424B2

    公开(公告)日：2005-02-01

    申请号：US10365677

    申请日：2003-02-12

    申请人： Thomas S. Deisboeck ,  E. Antonio Chiocca ,  John D. Wright

    发明人： Thomas S. Deisboeck ,  E. Antonio Chiocca ,  John D. Wright

    IPC分类号： C12M1/12 ,  C12M1/34 ,  C12Q1/02

    CPC分类号： C12M41/36 ,  C12M23/34

    摘要： The invention relates to devices and methods for growing cells in vitro in an enclosed device that allows for a three-dimensional measurement over time of both their proliferative and/or invasive properties. By growing the cells in an enclosed matrix that resembles the environment that the cells confront in vivo, the cells can divide, invade, and form branched networks as they do in living tissue, e.g., in an individual. The devices of the invention include a test chamber in which cells, e.g., tumor cells, are placed and permitted to divide and/or invade. Cells can be placed within an insert within a chamber of the device. A delivery chamber that connects to the test chamber enables the delivery of agents that can be studied, e.g., for their therapeutic potential. The assay devices of the invention can be used as model systems to study cancer biology and to evaluate the efficacy of anti-cancer therapeutics.

    摘要翻译： 本发明涉及用于在封闭装置中体外培养细胞的装置和方法，所述装置和方法允许随着时间的推移进行三维测量，包括它们的增殖和/或侵入性质。 通过将细胞生长在类似于细胞在体内所面临的环境的封闭的基质中，细胞可如在活组织中例如在个体中分开，侵入并形成分支的网络。 本发明的装置包括测试室，其中放置例如肿瘤细胞的细胞并允许分裂和/或侵入细胞。 细胞可以放置在装置的腔室内的插入物内。 连接到测试室的输送室能够输送可以研究的药剂，例如用于其治疗潜力。 本发明的测定装置可用作模型系统来研究癌症生物学并评估抗癌治疗剂的功效。

    Viral delivery system for infectious transfer of large genomic DNA inserts
    9.
    发明授权
    Viral delivery system for infectious transfer of large genomic DNA inserts 有权
    用于传染性大基因组DNA插入片段的病毒递送系统

    公开(公告)号：US07214515B2

    公开(公告)日：2007-05-08

    申请号：US10035216

    申请日：2002-01-04

    申请人： E. Antonio Chiocca ,  Yoshinaga Saeki ,  Richard Wade-Martins

    发明人： E. Antonio Chiocca ,  Yoshinaga Saeki ,  Richard Wade-Martins

    IPC分类号： C12N15/64 ,  C12N15/70 ,  C12N15/79

    CPC分类号： C12N7/00 ,  A61K38/177 ,  A61K38/45 ,  A61K48/00 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2710/16643 ,  C12N2800/108 ,  C12N2800/204 ,  C12N2800/206 ,  C12N2800/30

    摘要： The present invention relates to HSV-based amplicon vectors carrying a genomic DNA fragment, and methods of constructing and using the same. Included within the present invention is a method of converting any large capacity DNA cloning vector, such as a BAC or PAC, into an HSV amplicon or hybrid HSV amplicon using site-specific, or other types of recombination, so that genomic DNA inserts within the BAC or PAC clone can be delivered by infection to a cell, and efficiently expressed. The present invention also relates to a system for the rapid creation of viral vectors carrying transgenes of interest. This aspect of the invention is accomplished through recombination between: (a) a large-capacity cloning vector carrying a viral genome, and (b) a transfer vector containing the transgene of interest. Finally, an expression-ready genomic DNA library is disclosed.

    摘要翻译： 本发明涉及携带基因组DNA片段的基于HSV的扩增子载体及其构建和使用方法。 本发明中包括使用位点特异性或其他类型的重组将任何大容量DNA克隆载体（例如BAC或PAC）转化成HSV扩增子或杂交HSV扩增子的方法，使得基因组DNA插入到 BAC或PAC克隆可通过感染递送至细胞，并有效表达。 本发明还涉及快速产生携带转基因的病毒载体的系统。 本发明的这个方面是通过以下之间的重组来实现的：（a）携带病毒基因组的大容量克隆载体和（b）含有感兴趣的转基因的转移载体。 最后，公开了一种表达基因组DNA文库。