公开(公告)号：US06924109B2

公开(公告)日：2005-08-02

申请号：US10116437

申请日：2002-04-03

申请人： Thorsten Melcher ,  Keith Charles McFarland ,  Li Gan ,  Shiming Ye ,  Mirella Gonzalez-Zulueta

发明人： Thorsten Melcher ,  Keith Charles McFarland ,  Li Gan ,  Shiming Ye ,  Mirella Gonzalez-Zulueta

IPC分类号： C12N20060101 ,  C12N15/10 ,  C12N15/11 ,  C12P19/34 ,  C12Q1/68 ,  C07H21/04 ,  C12N1/20 ,  C12N15/00 ,  C12N15/63

CPC分类号： C12N15/1034 ,  C12N15/1072 ,  C12N15/1079 ,  C12N15/1093 ,  C12N15/111 ,  C12N2310/14 ,  C12N2320/12 ,  C12N2330/30

摘要： Methods for correlating genes and gene function are provided. Such methods generally involve selecting a candidate gene that appears to be correlated with a particular cellular state or activity and then validating the role of the candidate gene in establishment of such a cellular state or activity. Certain methods utilize RNA interference techniques in the validation process.

摘要翻译： 提供了相关基因和基因功能的方法。 这样的方法通常包括选择似乎与特定细胞状态或活性相关的候选基因，然后验证候选基因在建立这种细胞状态或活性中的作用。 某些方法在验证过程中使用RNA干扰技术。

公开(公告)号：US20050053936A1

公开(公告)日：2005-03-10

申请号：US10491509

申请日：2002-10-17

申请人： Thorsten Melcher ,  Keith McFarland ,  Li Gan ,  Shiming Ye ,  Mirella Gonzalez-Zulueta

发明人： Thorsten Melcher ,  Keith McFarland ,  Li Gan ,  Shiming Ye ,  Mirella Gonzalez-Zulueta

IPC分类号： C12N20060101 ,  C12N15/10 ,  C12N15/11 ,  C12P19/34 ,  C12Q1/68

CPC分类号： C12N15/1034 ,  C12N15/1072 ,  C12N15/1079 ,  C12N15/1093 ,  C12N15/111 ,  C12N2310/14 ,  C12N2320/12 ,  C12N2330/30

摘要： Methods for correlating genes and gene function are provided. Such methods generally involve selecting a candidate gene that appears to be correlated with a particular cellular state or activity and then validating the role of the candidate gene in establishment of such a cellular state or activity. Certain methods utilize RNA interference techniques in the validation process.

公开(公告)号：US06841351B2

公开(公告)日：2005-01-11

申请号：US10027807

申请日：2001-10-19

申请人： Li Gan ,  Mirella Gonzalez-Zulueta ,  Kristin Anton ,  Richa Wilson ,  Thorsten Melcher ,  Daniel Chin

发明人： Li Gan ,  Mirella Gonzalez-Zulueta ,  Kristin Anton ,  Richa Wilson ,  Thorsten Melcher ,  Daniel Chin

IPC分类号： C12N15/10 ,  C12Q1/68 ,  C07H21/04 ,  C12P19/34 ,  G01N33/566

CPC分类号： C12N15/1086

摘要： Methods for correlating genes and gene function are provided. Such methods generally involve selecting a candidate gene that appears to be correlated with a particular cellular state or activity and then validating the role of the candidate gene in establishment of such a cellular state or activity. Certain methods utilized RNA interference techniques in the validation process.

摘要翻译： 提供了相关基因和基因功能的方法。 这样的方法通常包括选择似乎与特定细胞状态或活性相关的候选基因，然后验证候选基因在建立这种细胞状态或活性中的作用。 某些方法在验证过程中使用RNA干扰技术。

公开(公告)号：US06670138B2

公开(公告)日：2003-12-30

申请号：US10001051

申请日：2001-10-31

申请人： Mirella Gonzalez-Zulueta ,  Mehrdad Shamloo ,  K.C. McFarland ,  Daniel Chin ,  Tadeusz Wieloch ,  Thorsten Melcher

发明人： Mirella Gonzalez-Zulueta ,  Mehrdad Shamloo ,  K.C. McFarland ,  Daniel Chin ,  Tadeusz Wieloch ,  Thorsten Melcher

IPC分类号： C12Q170

CPC分类号： C12Q1/6883 ,  C12Q2600/158

摘要： The present invention identifies a gene whose gene product provides a protective effect against neurological disorders or neuronal injuries. Further, the invention provides methods for diagnosing or assessing an individual's susceptibility to a neuronal injury such as stroke. Also provided are therapeutic methods for treating patients, and methods for prophylactically treating individuals susceptible to various neurological disorders or neuronal injuries. Additionally, the invention describes screening methods for identifying agents that can be administered to treat individuals that have suffered or are at risk to suffer such disorders or injuries.

摘要翻译： 本发明鉴定其基因产物对神经系统疾病或神经元损伤具有保护作用的基因。 此外，本发明提供了用于诊断或评估个体对诸如中风的神经元损伤的易感性的方法。 还提供了用于治疗患者的治疗方法，以及用于预防性治疗对各种神经障碍或神经元损伤敏感的个体的方法。 另外，本发明描述了用于鉴定可以被施用以治疗遭受或有遭受这种障碍或损伤风险的个体的药剂的筛选方法。

公开(公告)号：US20050260132A1

公开(公告)日：2005-11-24

申请号：US11097728

申请日：2005-03-31

申请人： Daniel Chin ,  Erik Foehr ,  Sabine Mueller ,  Thorsten Melcher

发明人： Daniel Chin ,  Erik Foehr ,  Sabine Mueller ,  Thorsten Melcher

IPC分类号： A61K39/395 ,  A61K47/48 ,  A61K51/00 ,  C07K16/28 ,  C07K16/30

CPC分类号： C07K16/3023 ,  A61K47/6819 ,  A61K47/6849 ,  A61K47/6855 ,  A61K47/6857 ,  A61K47/6861 ,  A61K47/6865 ,  A61K2039/505 ,  C07K16/28 ,  C07K16/3015 ,  C07K16/3038 ,  C07K16/3053 ,  C07K2317/73 ,  C07K2317/76 ,  C07K2317/77 ,  C07K2317/92

摘要： The present invention relates to a method of inhibiting growth of tumor cells which overexpress a receptor protein tyrosine phosphatase zeta (PTPζ) by treatment of the cells with antibodies which recognize PTPζ and/or inhibit PTPζ function. The present invention also provides compounds and pharmaceutically acceptable compositions for administration in the methods of the invention. The present invention also provides novel splice variants of protein PTPζ, PTPζ SM1 and PTPζ SM2. Nucleic acid probes specific for the spliced mRNA encoding these variants and affinity reagents specific for the novel proteins are also provided.

摘要翻译： 本发明涉及通过用识别PTPzeta和/或抑制PTPzeta功能的抗体处理细胞来抑制过表达受体蛋白酪氨酸磷酸酶ζ（PTPzeta）的肿瘤细胞生长的方法。 本发明还提供了用于本发明方法中给药的化合物和药学上可接受的组合物。 本发明还提供蛋白质PTPzeta，PTPzeta SM1和PTPzeta SM2的新型剪接变体。 还提供了针对编码这些变体的剪接mRNA特异性的核酸探针和对新蛋白特异的亲和试剂。

公开(公告)号：US07060275B2

公开(公告)日：2006-06-13

申请号：US10652981

申请日：2003-08-28

申请人： Sabine Mueller ,  Thorsten Melcher ,  Daniel Chin

发明人： Sabine Mueller ,  Thorsten Melcher ,  Daniel Chin

IPC分类号： A61K39/395 ,  A61K39/40 ,  C07H21/02

CPC分类号： A61K49/0002

摘要： The present invention relates to the use of proteins which are differentially expressed in primary brain tumor tissues, as compared to normal brain tissues, as biomolecular targets for brain tumor treatment therapies. Specifically, the present invention relates to the use of immunotherapeutic and immunoimaging agents which specifically bind to one or more of human proteins angiopoietin related protein 2 (ARP-2,) secreted protein acidic, rich in cysteine (SPARC,) c-met proto-oncogene (C-MET,) brevican (BEHAB,) CD-44 antigen (CD-44,) tetraspanin 3 (TSPN3,) pleiotrophin (PTN,) osteopontin (OPN,) vasoactive intestinal peptide receptor-2 (VIPR-2,) and receptor protein tyrosine phosphatase zeta (PTPζ) for the treatment and visualization of brain tumors in patients. The present invention also provides compounds and pharmaceutically acceptable compositions for administration in the methods of the invention. The present invention also provides novel splice variants of protein PTPζ, PTPζ SM1 and PTPζ SM2. Nucleic acid probes specific for the spliced mRNA encoding these variants and affinity reagents specific for the novel proteins are also provided.

公开(公告)号：US20060014801A1

公开(公告)日：2006-01-19

申请号：US11058734

申请日：2005-02-14

申请人： Michela Gallagher ,  Pauline Lund ,  Joel Selcher ,  Thorsten Melcher

发明人： Michela Gallagher ,  Pauline Lund ,  Joel Selcher ,  Thorsten Melcher

IPC分类号： A61K31/4439 ,  C07D403/02

CPC分类号： A61K31/19 ,  A61K31/5365 ,  A61K31/545 ,  A61K31/546 ,  C12Q1/6876 ,  C12Q2600/158

摘要： The invention provides methods for improving cognitive function in a subject by administering (R)-(−)-5-methyl-1-nicotinoyl-2-pyrazoline (MNP) or an analog to a subject in need of such treatment. The invention is useful for treatment of cognitive impairment such as mild cognitive impairment (MCI) as well as other conditions.

摘要翻译： 本发明提供了通过向需要这种治疗的受试者施用（R） - （ - ） - 5-甲基-1-烟酰基-2-吡唑啉（MNP）或类似物来提供改善受试者认知功能的方法。 本发明可用于治疗认知障碍，如轻度认知障碍（MCI）以及其他病症。

公开(公告)号：US20050074831A1

公开(公告)日：2005-04-07

申请号：US10386971

申请日：2003-03-11

申请人： Jasna Jerecic ,  Janice Williams ,  Jean Bucaria ,  Thorsten Melcher

发明人： Jasna Jerecic ,  Janice Williams ,  Jean Bucaria ,  Thorsten Melcher

IPC分类号： G01N33/94 ,  C12Q1/44

CPC分类号： G01N33/9406 ,  G01N2500/00

摘要： The present invention relates to the identification of a binding between NMDA receptor (NMDA-R) subunits and a serine/threonine protein phosphatase (PSTP), e.g., PP2A. The present invention provides methods for screening a PSTP agonist or antagonist that modulates NMDA-R signaling. The present invention also provide methods and compositions for treatment of disorders mediated by abnormal NMDA-R signaling.

摘要翻译： 本发明涉及鉴定NMDA受体（NMDA-R）亚基与丝氨酸/苏氨酸蛋白磷酸酶（PSTP）如PP2A之间的结合。 本发明提供了筛选调节NMDA-R信号传导的PSTP激动剂或拮抗剂的方法。 本发明还提供了用于治疗由NMDA-R异常信号传导介导的病症的方法和组合物。

公开(公告)号：US20050074400A1

公开(公告)日：2005-04-07

申请号：US10652981

申请日：2003-08-28

申请人： Sabine Mueller ,  Thorsten Melcher ,  Daniel Chin

发明人： Sabine Mueller ,  Thorsten Melcher ,  Daniel Chin

IPC分类号： A61K49/00 ,  A61K51/00 ,  A61K39/395

CPC分类号： A61K49/0002

摘要： The present invention relates to the use of proteins which are differentially expressed in primary brain tumor tissues, as compared to normal brain tissues, as biomolecular targets for brain tumor treatment therapies. Specifically, the present invention relates to the use of immunotherapeutic and immunoimaging agents which specifically bind to one or more of human proteins angiopoietin related protein 2 (ARP-2,) secreted protein acidic, rich in cysteine (SPARC,) c-met proto-oncogene (C-MET,) brevican (BEHAB,) CD-44 antigen (CD-44,) tetraspanin 3 (TSPN3,) pleiotrophin (PTN,) osteopontin (OPN,) vasoactive intestinal peptide receptor-2 (VIPR-2,) and receptor protein tyrosine phosphatase zeta (PTPζ) for the treatment and visualization of brain tumors in patients. The present invention also provides compounds and pharmaceutically acceptable compositions for administration in the methods of the invention. The present invention also provides novel splice variants of protein PTPζ, PTPζ SM1 and PTPζ SM2. Nucleic acid probes specific for the spliced mRNA encoding these variants and affinity reagents specific for the novel proteins are also provided.

摘要翻译： 本发明涉及与正常脑组织相比，在原发性脑肿瘤组织中差异表达的蛋白质作为脑肿瘤治疗疗法的生物分子靶标的用途。 具体地，本发明涉及特异性结合一种或多种人蛋白质的血管生成素相关蛋白2（ARP-2）分泌的蛋白质，富含半胱氨酸（SPARC）c-met原核蛋白的免疫治疗和免疫成像剂的用途。 癌基因（C-MET，）brevican（BEHAB）CD-44抗原（CD-44）四质蛋白3（TSPN3）多效素（PTN）骨桥蛋白（OPN）血管活性肠肽受体-2（VIPR-2） 和受体蛋白酪氨酸磷酸酶ζ（PTPzeta），用于治疗和观察患者脑肿瘤。 本发明还提供了用于本发明方法中给药的化合物和药学上可接受的组合物。 本发明还提供蛋白质PTPzeta，PTPzeta SM1和PTPzeta SM2的新型剪接变体。 还提供了针对编码这些变体的剪接mRNA特异性的核酸探针和对新蛋白特异的亲和试剂。

公开(公告)号：US06660483B1

公开(公告)日：2003-12-09

申请号：US09688078

申请日：2000-10-13

申请人： Kalev Kask ,  Thorsten Melcher ,  Daniel J. Chin

发明人： Kalev Kask ,  Thorsten Melcher ,  Daniel J. Chin

IPC分类号： C12Q100

CPC分类号： G01N33/573 ,  C12Q1/34 ,  G01N33/6896 ,  G01N2333/916

摘要： The invention relates to a human phospholipase C expressed in the brain (B-PLC) and induced in response to ischemic brain tissue. The inventions provides methods and reagents useful for diagnosis and treatment of hypoxic-ischemic brain insult such as stroke.

摘要翻译： 本发明涉及在脑中表达的人类磷脂酶C（B-PLC），并且响应缺血性脑组织诱导。 本发明提供了用于诊断和治疗缺氧缺血性脑损伤如中风的方法和试剂。