利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

10 条记录 (耗时 0.051 秒)

    Structural isomers of sc(Fv)2
    3.
    发明授权
    Structural isomers of sc(Fv)2 有权
    sc（Fv）2的结构异构体

    公开(公告)号：US09493569B2

    公开(公告)日：2016-11-15

    申请号：US11910117

    申请日：2006-03-31

    申请人： Tomoyuki Igawa ,  Hiroyuki Tsunoda ,  Akiko Koga ,  Yasufumi Kikuchi

    发明人： Tomoyuki Igawa ,  Hiroyuki Tsunoda ,  Akiko Koga ,  Yasufumi Kikuchi

    IPC分类号： C07K16/00 ,  C12P21/08 ,  A61K39/00 ,  A61K51/10 ,  C07K16/28

    CPC分类号： C07K16/2866 ,  C07K2317/622 ,  C07K2317/626 ,  C07K2317/75

    摘要： Structural isomers in sc(Fv)2 compositions of anti-human Mpl antibody and humanized anti-human Mpl antibody were separated, and the obtained structural isomers were cleaved at their linkers to confirm that the structural isomers are of single chain diabody type and bivalent scFv type. In addition, the agonistic activities of these structural isomers were revealed to be significantly different. Furthermore, the present inventors discovered that the content ratio of the structural isomers in sc(Fv)2 compositions could be regulated by altering temperature, modifying lengths of the linkers of sc(Fv)2, or amino acids in their variable regions.

    摘要翻译： 分离抗人Mpl抗体和人源化抗人Mpl抗体的sc（Fv）2组合物中的结构异构体，将得到的结构异构体在其接头上切割，以确认结构异构体是单链双抗体型和二价scFv 类型。 此外，这些结构异构体的激动活性显示出显着差异。 此外，本发明人发现sc（Fv）2组合物中的结构异构体的含量比可以通过改变温度，改变sc（Fv）2的接头的长度或其可变区中的氨基酸来调节。

    Structural Isomers of sc(Fv)2
    7.
    发明申请
    Structural Isomers of sc(Fv)2 有权
    sc（Fv）2的结构异构体

    公开(公告)号：US20090297501A1

    公开(公告)日：2009-12-03

    申请号：US11910117

    申请日：2006-03-31

    申请人： Tomoyuki Igawa ,  Hiroyuki Tsunoda ,  Akiko Koga ,  Yasufumi Kikuchi

    发明人： Tomoyuki Igawa ,  Hiroyuki Tsunoda ,  Akiko Koga ,  Yasufumi Kikuchi

    IPC分类号： A61K39/395 ,  C12Q1/00 ,  A61P43/00

    CPC分类号： C07K16/2866 ,  C07K2317/622 ,  C07K2317/626 ,  C07K2317/75

    摘要： Structural isomers in sc(Fv)2 compositions of anti-human Mpl antibody and humanized anti-human Mpl antibody were separated, and the obtained structural isomers were cleaved at their linkers to confirm that the structural isomers are of single chain diabody type and bivalent scFv type. In addition, the agonistic activities of these structural isomers were revealed to be significantly different. Furthermore, the present inventors discovered that the content ratio of the structural isomers in sc(Fv)2 compositions could be regulated by altering temperature, modifying lengths of the linkers of sc(Fv)2, or amino acids in their variable regions.

    摘要翻译： 分离抗人Mpl抗体和人源化抗人Mpl抗体的sc（Fv）2组合物中的结构异构体，将得到的结构异构体在其接头上切割，以确认结构异构体是单链双抗体型和二价scFv 类型。 此外，这些结构异构体的激动活性显示出显着差异。 此外，本发明人发现sc（Fv）2组合物中的结构异构体的含量比可以通过改变温度，改变sc（Fv）2的接头的长度或其可变区中的氨基酸来调节。

    METHOD OF PRODUCING FUSED PROTEIN
    9.
    发明申请
    METHOD OF PRODUCING FUSED PROTEIN 审中-公开
    生产融合蛋白的方法

    公开(公告)号：US20110065149A1

    公开(公告)日：2011-03-17

    申请号：US12310258

    申请日：2007-08-21

    申请人： Shigeo Kato ,  Yoichi Kumada ,  Tomomi Kawasaki ,  Yasufumi Kikuchi

    发明人： Shigeo Kato ,  Yoichi Kumada ,  Tomomi Kawasaki ,  Yasufumi Kikuchi

    IPC分类号： C12P21/00 ,  C07H21/04 ,  C12N15/63 ,  C12N1/21 ,  C07H21/00

    CPC分类号： C12N15/62 ,  C07K16/44 ,  C07K2317/622

    摘要： Described herein is a polynucleotide encoding a fusion protein comprising two or more polypeptide domains and a polypeptide linker joining the domains, wherein the sequence of the polynucleotide encoding the polypeptide linker is selected such that when the mRNA transcribed from the polynucleotide is translated in a host cell transfected with the polynucleotide, the translation rate of the mRNA region encoding the polypeptide linker is slower than the translation rate of the mRNA region encoding the polypeptide domain immediately upstream thereof. Also provided are a vector transfected with the polynucleotide of the present invention so that the polynucleotide can be expressed in the host cell; a host cell transformed by that vector; and a process for producing a fusion protein comprising culturing the host cell, and recovering the fusion protein thus produced.

    摘要翻译： 本文描述了编码融合蛋白的多核苷酸，其包含两个或多个多肽结构域和连接结构域的多肽接头，其中选择编码多肽接头的多核苷酸序列，使得当从多核苷酸转录的mRNA在宿主细胞中翻译时 用多核苷酸转染，编码多肽接头的mRNA区的翻译速率比编码紧邻其上游的多肽结构域的mRNA区的翻译速度慢。 还提供了用本发明的多核苷酸转染的载体，使得多核苷酸可以在宿主细胞中表达; 由该载体转化的宿主细胞; 以及生产融合蛋白的方法，包括培养宿主细胞，并回收由此产生的融合蛋白。