公开(公告)号：US06660504B2

公开(公告)日：2003-12-09

申请号：US10042280

申请日：2002-01-11

申请人： Tsuneo Yamane ,  Yugo Iwasaki ,  Yukiko Mizumoto ,  Masaaki Kasai ,  Takahiro Okada

发明人： Tsuneo Yamane ,  Yugo Iwasaki ,  Yukiko Mizumoto ,  Masaaki Kasai ,  Takahiro Okada

IPC分类号： C12N916

CPC分类号： C12P7/6481 ,  C12P13/00 ,  C12P13/06

摘要： The present invention provides a process for preparing a phospholipid in an aqueous system in which hydrolysis is extremely controlled, and the synthetic yield is improved. A process for exchanging a base of a phospholipid as a raw material by subjecting the phospholipid to the action of phospholipase D in the presence of a receptor having a hydroxyl group, in which the reaction is carried out in an aqueous system, a phospholipid adsorbed on a carrier is used as a raw material phospholipid, and the receptor and the phospholipase D are used in free forms.

摘要翻译： 本发明提供了一种在水性体系中制备磷脂的方法，其中水解被极度控制，并且合成产率提高。1.一种通过使磷脂作用磷脂酶作为原料来交换磷脂的碱的方法 在具有羟基的受体的存在下，其中反应在水性体系中进行，吸附在载体上的磷脂用作原料磷脂，受体和磷脂酶D以游离形式使用 。

公开(公告)号：US20050250189A1

公开(公告)日：2005-11-10

申请号：US11117489

申请日：2005-04-29

申请人： Tsuneo Yamane ,  Yugo Iwasaki ,  Namiko Takahashi ,  Takaya Yamamoto ,  Takahiro Okada

发明人： Tsuneo Yamane ,  Yugo Iwasaki ,  Namiko Takahashi ,  Takaya Yamamoto ,  Takahiro Okada

IPC分类号： C12P13/06 ,  C07F9/10 ,  C12P13/04

CPC分类号： C07F9/10

摘要： Particularly in the recovery and reuse of unreacted serine after the synthesis of phosphatidylserine by the transfer reaction of a phosphatidyl group with phospholipase D, a simple process for recovering serine in which a material inhibitory to the synthesis of phosphatidylserine can be efficiently removed is provided. The present invention provides the following processes for recovering serine. A process for recovering unreacted serine from a reaction system after the synthesis of phosphatidylserine by the transfer reaction of a phosphatidyl group with phospholipase D, comprising adding a polar organic solvent or a mixture of a polar organic solvent and water to the unreacted serine containing water-soluble fraction in which the phosphatidylserine has been removed from a reaction mixture in the reaction system and washing the fraction with the added solvent or the mixture of the solvent and water to obtain the serine. A process for recovering the serine described above, wherein the synthesis of phosphatidylserine by the transfer reaction of a phosphatidyl group with phospholipase D is performed in an aqueous system in the absence of an organic solvent. A process for recovering the serine described above, wherein the synthesis of phosphatidylserine by the transfer reaction of a phosphatidyl group with phospholipase D is performed in a two-phase system with an organic solvent and water.

摘要翻译： 特别是在通过磷脂酰基与磷脂酶D的转移反应合成磷脂酰丝氨酸后的未反应丝氨酸的回收和再利用中，提供了一种简单的回收丝氨酸的方法，其中可以有效地除去抑制磷脂酰丝氨酸合成的材料。 本发明提供以下回收丝氨酸的方法。 通过磷脂酰基与磷脂酶D的转移反应合成磷脂酰丝氨酸后，从反应体系中回收未反应的丝氨酸的方法，其包括将极性有机溶剂或极性有机溶剂与水的混合物添加到含有水溶性聚合物的未反应的丝氨酸中， 其中已经从反应体系中的反应混合物中除去磷脂酰丝氨酸并用添加的溶剂或溶剂和水的混合物洗涤级分以获得丝氨酸的可溶性级分。 一种回收上述丝氨酸的方法，其中通过磷脂酰基与磷脂酶D的转移反应合成磷脂酰丝氨酸是在不存在有机溶剂的情况下在水性体系中进行的。 一种回收上述丝氨酸的方法，其中通过磷脂酰基与磷脂酶D的转移反应合成磷脂酰丝氨酸在具有有机溶剂和水的两相体系中进行。

公开(公告)号：US4409943A

公开(公告)日：1983-10-18

申请号：US421581

申请日：1982-09-22

申请人： Satoshi Komurasaki ,  Atsushi Ueda ,  Tsuneo Yamane

发明人： Satoshi Komurasaki ,  Atsushi Ueda ,  Tsuneo Yamane

IPC分类号： F02P5/145 ,  F02P5/155 ,  F02P5/04

CPC分类号： F02P5/1455 ,  F02P5/1555 ,  Y02T10/46

摘要： An ignition timing correcting system for generating a reference signal at a reference angular position of a rotating internal combustion engine, and having an ignition signal leading it by a constant phase. In response to a control signal from a movable tap on a potentiometer, the ignition signal is retarded in phase to form a retarded ignition signal. The phase comparison of the two signals produces an advance or a retardation signal formed of a pulse train having a pulse width corresponding to the phase difference there-between. During the idling of the engine, the correcting signal drives an electric reversible motor in either a forward or reverse direction so as to move the tap on the potentiometer to adjust the control signal after the correcting signal has been converted to a driving pulse having a longer pulse width when correcting in a direction to correct an advance of said signal than the pulse width when correcting in a direction to correct a retardation of said signal with respect to the phase difference.

摘要翻译： 一种点火正时校正系统，用于在旋转内燃机的基准角度位置处产生参考信号，并且具有使恒定相位引导的点火信号。 响应于来自电位器上的可动抽头的控制信号，点火信号被相位延迟以形成延迟点火信号。 两个信号的相位比较产生由具有对应于其之间的相位差的脉冲宽度的脉冲串形成的提前或延迟信号。 在发动机怠速期间，校正信号沿正向或反向驱动电动可逆电动机，以便在电位计上移动水龙头以在校正信号已被转换成具有较长时间的驱动脉冲之后调节控制信号 当在校正所述信号相对于所述相位差的延迟的方向进行校正时，在校正所述信号的前进方向上的脉冲宽度的脉冲宽度。

公开(公告)号：US5168852A

公开(公告)日：1992-12-08

申请号：US798584

申请日：1991-11-26

申请人： Teruhiko Moriguchi ,  Yutaka Okaue ,  Manabu Hirai ,  Osamu Matsumoto ,  Tsuneo Yamane

发明人： Teruhiko Moriguchi ,  Yutaka Okaue ,  Manabu Hirai ,  Osamu Matsumoto ,  Tsuneo Yamane

IPC分类号： F02D9/02

CPC分类号： F02D9/02 ,  F02D2009/0269

摘要： A return spring assembly for use in an engine intake throttle valve return device comprising a spring holder sleeve (17) including an inner and outer sleeves (18,20) placed over a throttle valve shaft (3) and connected at one end to each other so that the inner and outer sleeves (18,20) are spacedly and concentrically held. Inner and outer torsion coil springs (8,9) are supported on the inner and outer sleeves (18,20), respectively, and connected between an intake barrel (1) and the valve shaft (3) for urging the throttle valve (2) to its closed position. An eccentric radial flange (22) which may be circular, oval or polygonal is provided on the outer sleeve (20) for holding the outer torsion coil spring (9). The eccentric flange (22) extends continuously over the entire circumference of the outer sleeve (20) and has a maximum outer diametrical dimension substantially equal to the inner diameter of the outer torsion coil spring (9). The eccentric flange (22) is eccentric with respect to the outer sleeve (20) by an amount effective to prevent the outer coil spring (9) from being disclocated from the outer sleeve (20).

摘要翻译： 一种用于发动机进气节流阀返回装置中的复位弹簧组件，包括弹簧保持器套筒（17），弹簧保持器套筒（17）包括放置在节流阀轴（3）上并且一端彼此连接的内部和外部套筒（18,20） 使得内部和外部套筒（18,20）间隔和同心地保持。 内外扭力螺旋弹簧（8,9）分别支撑在内套筒和外套筒（18,20）上，连接在进气筒（1）和阀轴（3）之间，用于推动节流阀（2） ）到其关闭位置。 在外套筒（20）上设置可以是圆形，椭圆形或多边形的偏心径向凸缘（22），用于保持外扭力螺旋弹簧（9）。 偏心凸缘（22）在外套筒（20）的整个圆周上连续延伸，并具有基本上等于外扭力螺旋弹簧（9）的内径的最大外直径尺寸。 偏心凸缘（22）相对于外套筒（20）偏心一定量，以有效地防止外螺旋弹簧（9）与外套筒（20）分离。

公开(公告)号：US4373487A

公开(公告)日：1983-02-15

申请号：US249459

申请日：1981-03-31

申请人： Satoshi Komurasaki ,  Tsuneo Yamane

发明人： Satoshi Komurasaki ,  Tsuneo Yamane

IPC分类号： F02P5/155 ,  F02P5/04

CPC分类号： F02P5/1551 ,  F02P5/1558 ,  Y02T10/46

摘要： An ignition timing correcting system generate a reference signal at a reference angular position of a rotating internal combustion engine and an ignition signal leading it by a constant phase. A phase shifter normally responds to a control signal from a movable tap on a potentiometer, to retard the ignition signal to form a retarded ignition signal. The phase comparison of the two signals produces an advance or a retardation correcting signal formed of a pulse train having a pulse-width corresponding to a phase difference between the two ignition signals. During the idling of the engine, the correcting signal drives forwardly or reversely an electric reversible motor to move the tap on the potentiometer to adjust the control signal. During the warm-up of the engine, the phase shifter is supplied with the sum of the control signal and a signal with a predetermined voltage to further retard the ignition signal.

摘要翻译： 点火正时校正系统在旋转内燃机的基准角度位置产生参考信号，并将点火信号引导到恒定相位。 移相器通常响应来自电位器上的可动抽头的控制信号，以延迟点火信号以形成延迟的点火信号。 两个信号的相位比较产生由具有对应于两个点火信号之间的相位差的脉冲宽度的脉冲串形成的提前或延迟校正信号。 在发动机怠速期间，校正信号向前或向后驱动电动可逆电动机以使电位器上的水龙头移动以调节控制信号。 在发动机的预热期间，向移相器提供控制信号和具有预定电压的信号之和，以进一步延迟点火信号。

公开(公告)号：US06207378B1

公开(公告)日：2001-03-27

申请号：US09136411

申请日：1998-08-19

申请人： Tsuneo Yamane ,  Hideo Nakano ,  Masashi Ouchi ,  Reiko Okumura ,  Satoshi Sekiguchi

发明人： Tsuneo Yamane ,  Hideo Nakano ,  Masashi Ouchi ,  Reiko Okumura ,  Satoshi Sekiguchi

IPC分类号： C12Q168

CPC分类号： C12P21/02 ,  C12Q1/686 ,  C12Q2527/143

摘要： A method for amplifying desired nucleic acid molecules by PCR which comprises the steps of isolating and purifying a group of nucleic acid molecules including desired nucleic acid molecules to be amplified, then carrying out PCR while establishing such a condition that the nucleic acid molecules capable of being amplified, except for primers, present in a PCR reaction solution are constituted by only the desired nucleic acid molecules and that the concentration of each primer used is limited to a level of not more than 100 nM; a method for producing a protein in a cell-free protein-synthesis system containing a cell-free extract which comprises using the nucleic acid of a single kind produced by the method described above and a method for establishing a protein library which comprises the steps of separately carrying out the method for amplifying desired nucleic acid molecules by PCR to obtain at least two kinds of nucleic acid molecules and separately carrying out the foregoing method for producing a protein using each of the at least two kinds of the amplified nucleic acids as a template to thus establish a protein library which comprises at least two kinds of proteins encoded by the resulting at least two nucleic acids respectively.

摘要翻译： 一种通过PCR扩增所需核酸分子的方法，该方法包括以下步骤：分离和纯化包括要扩增的所需核酸分子的核酸分子组，然后进行PCR，同时建立这样的条件，使得核酸分子能够 存在于PCR反应溶液中的引物除了扩增，仅由所需的核酸分子构成，使用的引物的浓度限制在不超过100nM的水平; 一种含有无细胞提取物的无细胞蛋白质合成系统中的蛋白质的制备方法，该方法包括使用通过上述方法制备的单一种核酸和建立蛋白质文库的方法，该方法包括以下步骤： 分别进行通过PCR扩增所需核酸分子的方法，以获得至少两种核酸分子，并且使用所述至少两种扩增的核酸作为模板分别进行上述制备蛋白质的方法 从而建立蛋白质文库，该蛋白质文库分别包含由所得至少两个核酸编码的至少两种蛋白质。

公开(公告)号：US4374510A

公开(公告)日：1983-02-22

申请号：US249458

申请日：1981-03-31

申请人： Satoshi Komurasaki ,  Atsushi Ueda ,  Tsuneo Yamane

发明人： Satoshi Komurasaki ,  Atsushi Ueda ,  Tsuneo Yamane

IPC分类号： F02P5/155 ,  F02P5/152 ,  F02P5/153

CPC分类号： F02P5/152 ,  Y02T10/46

摘要： An ignition timing correcting system generates a reference signal at a reference angular position of a rotating internal combustion engine and an ignition signal leading it by a constant phase. In response to a control signal from a movable tap on a potentiometer, the ignition signal is normally retarded in phase to form a retarded ignition signal. The phase comparison of the two signals produces an advance or a retardation signal formed of a pulse train having a pulse-width corresponding to a phase difference between the two ignition signals. During the idling of the engine, the correcting signal drives forwardly or reversely an electric reversible motor to move the tap on the potentiometer to adjust the control signal. During the knocking of the engine, a knocking sensed signal is added to the control signal from the potentiometer to further retard the ignition signal. During the idling of the engine, the knocking sensed signal is disabled to retard the ignition signal.

摘要翻译： 点火正时校正系统在旋转内燃机的基准角度位置产生参考信号，并且使点火信号通过恒定相位产生。 响应于来自电位器上的可动抽头的控制信号，点火信号通常相位延迟以形成延迟点火信号。 两个信号的相位比较产生由具有对应于两个点火信号之间的相位差的脉冲宽度的脉冲串形成的提前或延迟信号。 在发动机怠速期间，校正信号向前或向后驱动电动可逆电动机以使电位器上的水龙头移动以调节控制信号。 在发动机爆震期间，将来自电位器的控制信号中加入一个爆震感测信号，以进一步延迟点火信号。 在发动机怠速期间，敲击感测信号被禁止以延迟点火信号。

公开(公告)号：US5200332A

公开(公告)日：1993-04-06

申请号：US759919

申请日：1991-09-13

申请人： Tsuneo Yamane ,  Shunsaku Ueda ,  Shigeki Imagawa ,  Torakazu Tahara ,  Yoshiharu Tokunaga ,  Hiroyuki Iesaka ,  Teizi Urakami

发明人： Tsuneo Yamane ,  Shunsaku Ueda ,  Shigeki Imagawa ,  Torakazu Tahara ,  Yoshiharu Tokunaga ,  Hiroyuki Iesaka ,  Teizi Urakami

IPC分类号： C08G63/06 ,  C12P7/62

CPC分类号： C08G63/06 ,  C12P7/625 ,  Y10S435/822 ,  Y10S435/829 ,  Y10S435/831

摘要： Disclosed is a process for the preparation of a copolymer, which comprises propagating cells of a bacterium having a capacity of producing poly-3-hydroxybutyrate mainly at a former stage, synthesizing and accumulating in the cells a copolymer comprising D-3-hydroxybutyrate and D-3-hydroxyvalerate by contacting the bacterium with a mixture of a carbon source utilizable by the bacterium and a primary alcohol having 3 to 7 carbon atoms, or with a primary alcohol having 3 to 7 carbon atoms, at a latter stage, and recovering the copolymer from the cells. According to this process, a copolymer comprising D-3-hydroxybutyrate and D-3-hydroxyvalerate can be manufactured in a large quantity at a low cost.

公开(公告)号：US06905850B2

公开(公告)日：2005-06-14

申请号：US10311350

申请日：2001-07-02

申请人： Roxana Irimescu ,  Kiyomi Furihata ,  Kazuhiko Hata ,  Tsuneo Yamane

发明人： Roxana Irimescu ,  Kiyomi Furihata ,  Kazuhiko Hata ,  Tsuneo Yamane

IPC分类号： C11C3/08 ,  C11C3/10 ,  C12P7/64

CPC分类号： C12P7/6445 ,  C12P7/6472

摘要： The invention provides a process for producing 2-monoglycerides (2-MG) or triglycerides (TG), particularly triglycerides having polyunsaturated fatty acid (PUFA) residues in the sn-2 positions, at an extremely high purity and a high efficiency.The 2-MG is produced by conducting alcoholysis of a starting TG with a first 1,3-lipase. Then, the TG is produced by introducing fatty acid residues to the 1- and 3-positions of the produced 2-MG by using a second 1,3-lipase. The fatty acid residue in the 2-posiiton is preferably a residue of DHA, EPA or ARA. The “first lipase” refers to one which is selective for the 1- and 3-positions and can act even on a long-chain fatty acid in alcoholysis, while the “second lipase” refers to one which is selective for the 1- and 3-positions and can produce a TG through the reaction of a 2-MG with a fatty acid ester or a free fatty acid.

摘要翻译： 本发明提供了以非常高的纯度和高效率生产2-单甘油酯（2-MG）或甘油三酸酯（TG），特别是在sn-2位具有多不饱和脂肪酸（PUFA）残基的甘油三酯）的方法。 通过用第一种1,3-脂肪酶进行起始TG的醇解来制备2-MG。 然后，通过使用第二种1,3-脂肪酶将所产生的2-MG的1-和3-位引入脂肪酸残基来产生TG。 2-位的脂肪酸残基优选为DHA，EPA或ARA的残基。 “第一脂肪酶”是指对于1-和3-位选择性的那些，并且甚至可以在醇解中的长链脂肪酸上起作用，而“第二脂肪酶”是指对于1-和 3-位，并且可以通过2-MG与脂肪酸酯或游离脂肪酸的反应产生TG。

公开(公告)号：US5100787A

公开(公告)日：1992-03-31

申请号：US559047

申请日：1990-07-30

申请人： Shoichi Shimizu ,  Tsuneo Yamane ,  Dongxiu Li ,  Lekh R. Juneja

发明人： Shoichi Shimizu ,  Tsuneo Yamane ,  Dongxiu Li ,  Lekh R. Juneja

IPC分类号： C12P9/00 ,  C12P7/64

CPC分类号： C12P7/6481

摘要： A method for preparing highly purified phosphatidylinositol comprises the steps of treating a mixed phospholipid with a phospholipase D, then treating the product with an alkali or acidic phosphatase and separating unreated phosphatidylinositol from the reaction mixture. The method makes it possible to isolate phosphatidylinositol useful in various fields in a high purity in the order of 80 to 99%.

摘要翻译： 制备高纯度磷脂酰肌醇的方法包括用磷脂酶D处理混合磷脂，然后用碱或酸性磷酸酶处理产物并从反应混合物中分离未反应的磷脂酰肌醇的步骤。 该方法可以分离出高纯度各种领域中有用的磷脂酰肌醇的量级为80-99％。