-
公开(公告)号：US06825009B2
公开(公告)日：2004-11-30
申请号：US10105101
申请日：2002-03-22
申请人： Vince P. Stanton, Jr. , Jia Liu Wolfe , Tomohiko Kawate , Gregory L. Verdine , Jeffrey Olson , Colin W Dykes
发明人： Vince P. Stanton, Jr. , Jia Liu Wolfe , Tomohiko Kawate , Gregory L. Verdine , Jeffrey Olson , Colin W Dykes
IPC分类号： C12Q168
CPC分类号： C12Q1/6858 , C07H19/10 , C07H19/14 , C07H19/20 , C07H21/00 , C12N9/1252 , C12N15/11 , C12P19/34 , C12Q1/6827 , C12Q1/683 , C12Q1/6872 , C12Q2525/117 , C12Q2523/107 , C12Q2527/107 , C12Q2565/627 , C12Q2525/101
摘要： The present invention relates to methods for the detection of polymorphism in polynucleotides by using hybridization of fragments of segments of a polynucleotide suspected of containing a polymorphism with an oligonucleotide having a sequence complementary to a fragment identifying the polymorphism and subsequent detection of incorporated labels in the oligonucleotide-fragment duplex.
摘要翻译： 本发明涉及通过使用疑似含有多态性的多核苷酸的片段的片段与具有与鉴定多态性的片段互补的序列的寡核苷酸杂交并随后检测寡核苷酸中的并入标记的方法来检测多核苷酸中的多态性的方法 碎片双工。
-
公开(公告)号：US06500650B1
公开(公告)日：2002-12-31
申请号：US09655104
申请日：2000-09-05
IPC分类号： C12Q168
CPC分类号： C12Q1/6858 , C07H19/10 , C07H19/14 , C07H19/20 , C07H21/00 , C12N9/1252 , C12N15/11 , C12P19/34 , C12Q1/6827 , C12Q1/683 , C12Q1/6872 , C12Q2525/117 , C12Q2523/107 , C12Q2527/107 , C12Q2565/627 , C12Q2525/101
摘要： The present invention relates to methods for the detection of polymorphism in polynucleotides by using hybridization of fragments of segments of a polynucleotide suspected of containing a polymorphism with an oligonucleotide having a sequence complementary to a fragment identifying the polymorphism and subsequent detection of incorporated labels in the oligonucleotide-fragment duplex.
摘要翻译： 本发明涉及通过使用疑似含有多态性的多核苷酸的片段的片段与具有与鉴定多态性的片段互补的序列的寡核苷酸杂交并随后检测寡核苷酸中的并入标记的方法来检测多核苷酸中的多态性的方法 碎片双工。
-
公开(公告)号：US06994998B1
公开(公告)日：2006-02-07
申请号：US10043615
申请日：2002-01-08
申请人： Vincent P. Stanton, Jr. , Jia Liu Wolfe , Tomohiko Kawate , Charles Allerson , Gregory L. Verdine
发明人： Vincent P. Stanton, Jr. , Jia Liu Wolfe , Tomohiko Kawate , Charles Allerson , Gregory L. Verdine
CPC分类号： C07H19/10 , C07H19/14 , C07H19/20 , C07H21/00 , C12N9/1252 , C12N15/11 , C12P19/34 , C12Q1/6827 , C12Q1/6872 , C12Q2565/627 , C12Q2525/101 , C12Q2523/107
摘要： The present invention relates to methods for the detection of polymorphisms by substituting a base-modified nucleotide for a natural nucleotide in a polynucleotide. The base-modified nucleotide renders the polynucleotide more susceptible to cleavage at the sites of its incorporation than site consisting of natural nucleotides. The fragments obtained are then analyzed to determine the presence or absence of a polymorphism.
摘要翻译： 本发明涉及通过用多核苷酸中的天然核苷酸取代碱基修饰的核苷酸来检测多态性的方法。 碱基修饰的核苷酸使多核苷酸比其天然核苷酸组成的位点更易于在其掺入位点处切割。 然后分析获得的片段以确定多态性的存在或不存在。
-
公开(公告)号：US06582923B2
公开(公告)日：2003-06-24
申请号：US10104818
申请日：2002-03-22
IPC分类号： C12Q168
CPC分类号： C07H19/10 , C07H19/14 , C07H19/20 , C07H21/00 , C12N9/1252 , C12N15/11 , C12P19/34 , C12Q1/6827 , C12Q1/6872 , C12Q2565/627 , C12Q2525/101 , C12Q2523/107
摘要： The present invention relates to a method of synthesizing a polynucleotide with incorporation of a modified nucleotide.
摘要翻译： 本发明涉及合成多核苷酸并掺入经修饰的核苷酸的方法。
-
公开(公告)号：US06440705B1
公开(公告)日：2002-08-27
申请号：US09394457
申请日：1999-09-10
IPC分类号： C12P1934
CPC分类号： C07H19/10 , C07H19/14 , C07H19/20 , C07H21/00 , C12N9/1252 , C12N15/11 , C12P19/34 , C12Q1/6827 , C12Q1/6872 , C12Q2565/627 , C12Q2525/101 , C12Q2523/107
摘要： The present invention relates to methods for the analysis of polynucleotides including detection of variance in nucleotide sequence without the need for full sequence determination, full sequence determination of a polynucleotide, genotyping of DNA and labeling a polynucleotide fragment during the process of cleaving it into fragments.
-
公开(公告)号：US06458945B1
公开(公告)日：2002-10-01
申请号：US09709596
申请日：2000-11-09
IPC分类号： C12Q168
CPC分类号： C07H19/10 , C07H19/14 , C07H19/20 , C07H21/00 , C12N9/1252 , C12N15/11 , C12P19/34 , C12Q1/6827 , C12Q1/6872 , C12Q2565/627 , C12Q2525/101 , C12Q2523/107
摘要： The present invention relates to methods for the analysis of polynucleotides including detection of variance in nucleotide sequence without the need for full sequence determination, full sequence determination of a polynucleotide, genotyping of DNA and labeling a polynucleotide fragment during the process of cleaving it into fragments.
摘要翻译： 本发明涉及用于分析多核苷酸的方法，包括在不需要全序列测定的情况下检测核苷酸序列的方差，多核苷酸的全序列测定，DNA的基因分型以及在将其切割成片段的过程中标记多核苷酸片段。
-
7.发明授权Base-modified nucleotides and cleavage of polynucleotides incorporating them 有权碱基修饰的核苷酸和包含它们的多核苷酸的切割公开(公告)号：US06610492B1
公开(公告)日：2003-08-26
申请号：US10043511
申请日：2002-01-08
申请人： Vincent P. Stanton, Jr. , Jia Liu Wolfe , Tomohiko Kawate , Charles Allerson , Gregory L. Verdine
发明人： Vincent P. Stanton, Jr. , Jia Liu Wolfe , Tomohiko Kawate , Charles Allerson , Gregory L. Verdine
IPC分类号： C12Q168
CPC分类号： C07H19/10 , C07H19/14 , C07H19/20 , C07H21/00 , C12N9/1252 , C12N15/11 , C12P19/34 , C12Q1/6827 , C12Q1/6872 , C12Q2565/627 , C12Q2525/101 , C12Q2523/107
摘要： The present invention is directed to novel base-modified nucleotides and methods for their use in the preparation and cleavage of modified polynucleotides.
摘要翻译： 本发明涉及新的碱基修饰的核苷酸及其用于制备和切割修饰的多核苷酸的方法。
-
公开(公告)号：US06855500B2
公开(公告)日：2005-02-15
申请号：US10107748
申请日：2002-03-26
CPC分类号： C07H19/10 , C07H19/14 , C07H19/20 , C07H21/00 , C12N9/1252 , C12N15/11 , C12P19/34 , C12Q1/6827 , C12Q1/6872 , C12Q2565/627 , C12Q2525/101 , C12Q2523/107
摘要： The present invention relates to a method for genotyping a diploid organism to detect single nucleotide polymorphisms (SNPs) using modified nucleotides or nucleotide residues substituted with fluorescent groups.
摘要翻译： 本发明涉及使用修饰的核苷酸或被荧光基团取代的核苷酸残基来检测二核生物体以检测单核苷酸多态性（SNP）的方法。
-
公开(公告)号：US20050053972A1
公开(公告)日：2005-03-10
申请号：US10839686
申请日：2004-05-04
申请人： Vince Stanton , Jia Wolfe , Tomohiko Kawate , Gregory Verdine , Jeffrey Olson
发明人： Vince Stanton , Jia Wolfe , Tomohiko Kawate , Gregory Verdine , Jeffrey Olson
IPC分类号： G01N27/62 , C07H19/04 , C07H19/10 , C07H19/14 , C07H19/20 , C07H21/00 , C12N9/12 , C12N15/09 , C12N15/11 , C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6858 , C07H19/10 , C07H19/14 , C07H19/20 , C07H21/00 , C12N9/1252 , C12N15/11 , C12P19/34 , C12Q1/6827 , C12Q1/683 , C12Q1/6872 , C12Q2525/117 , C12Q2523/107 , C12Q2527/107 , C12Q2565/627 , C12Q2525/101
摘要： The present invention relates to methods for the detection of polymorphism in polynucleotides by using hybridization of fragments of segments of a polynucleotide suspected of containing a polymorphism with an oligonucleotide having a sequence complementary to a fragment identifying the polymorphism and subsequent detection of incorporated labels in the oligonucleotide-fragment duplex.
摘要翻译： 本发明涉及通过使用疑似含有多态性的多核苷酸的片段的片段与具有与鉴定多态性的片段互补的序列的寡核苷酸杂交并随后检测寡核苷酸中的并入标记的方法来检测多核苷酸中的多态性的方法 碎片双工。
-
-
-
-
-
-
-
-
搜索结果
9 条记录 (耗时 0.053 秒)
