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    Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove binders
    6.
    发明申请
    Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove binders 审中-公开
    使用与小沟结合物缀合的寡核苷酸的杂交和错配鉴别

    公开(公告)号：US20050064463A1

    公开(公告)日：2005-03-24

    申请号：US10870578

    申请日：2004-06-16

    申请人： Joel Hedgpeth ,  Irina Afonina ,  Igor Kutyavin ,  Eugeny Lukhtanov ,  Evgeniy Belousov ,  Rich Meyer

    发明人： Joel Hedgpeth ,  Irina Afonina ,  Igor Kutyavin ,  Eugeny Lukhtanov ,  Evgeniy Belousov ,  Rich Meyer

    IPC分类号： C12N15/09 ,  C07H19/23 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/68 ,  C12Q1/6813 ,  G01N21/78 ,  G01N33/483 ,  G01N33/53 ,  G01N33/566 ,  C12P19/34

    CPC分类号： C07H21/00 ,  C07H19/23 ,  C12Q1/6813 ,  C12Q2527/107 ,  C12Q2525/197

    摘要： Conjugates between a minor groove binding molecule, such as the trimer of 1,2-dihydro-(3H)-pyrrolo[3,2-e]indole-7-carboxylate (CDPI3), and an oligonucleotide form unusually stable hybrids with complementary target sequences, in which the tethered CDPI3 group resides in the minor groove of the duplex. These conjugates can be used as probes and primers. Due to their unusually high binding affinity, conjugates as short as 8-mers can be used as amplification primers with high specificity and efficiency. MGB conjugation also increases the discriminatory power of short oligonucleotides, providing enhanced detection of nucleotide sequence mismatches by short oligonucleotides. The MGB-conjugated probes and primers described herein facilitate various analytic and diagnostic procedures, such as amplification reactions, PCR, detection of single-nucleotide polymorphisms, gene hunting, differential display, fluorescence energy transfer, hydrolyzable probe assays and others; by allowing the use of shorter oligonucleotides, which have higher specificity and better discriminatory power.

    摘要翻译： 小沟结合分子（例如1,2-二氢 - （3H） - 吡咯并[3,2-e]吲哚-7-羧酸酯（CDPI3）的三聚体和寡核苷酸之间的缀合物形成非常稳定的具有互补靶的杂交体 序列，其中系留的CDPI3基团位于双链体的小沟槽中。 这些缀合物可以用作探针和引物。 由于其非常高的结合亲和力，短至8聚体的缀合物可以用作高特异性和高效的扩增引物。 MGB缀合还增加了短寡核苷酸的鉴别能力，提供了通过短寡核苷酸增强核苷酸序列错配检测。 本文所述的MGB缀合的探针和引物有助于各种分析和诊断程序，如扩增反应，PCR，单核苷酸多态性检测，基因敲除，差异显示，荧光能量转移，水解性探针测定等。 通过允许使用具有更高特异性和更好的辨别力的较短的寡核苷酸。

    Hybridization-triggered fluorescent detection of nucleic acids
    8.
    发明申请
    Hybridization-triggered fluorescent detection of nucleic acids 失效
    杂交荧光检测核酸

    公开(公告)号：US20070166723A1

    公开(公告)日：2007-07-19

    申请号：US11242977

    申请日：2005-10-03

    申请人： Robert Dempcy ,  Irina Afonina ,  Nicolaas Vermeulen

    发明人： Robert Dempcy ,  Irina Afonina ,  Nicolaas Vermeulen

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C07H21/00 ,  C12Q1/6816 ,  C12Q2563/107 ,  C12Q2565/107 ,  C12Q2563/173

    摘要： Compositions and methods for fluorescent detection of nucleic acids are provided. The compositions can be detected by fluorescence when hybridized to a nucleic acid containing a target sequence, but are non-fluorescent in the non-hybridized state. Alternatively, the fluorescence properties of the compositions change in a detectable manner upon hybridization to a nucleic acid containing a target sequence. Methods for synthesis and methods of use of the compositions are also provided.

    摘要翻译： 提供了核酸荧光检测的组合物和方法。 当与含有靶序列的核酸杂交时，可以通过荧光检测组合物，但是在非杂交状态下是非荧光的。 或者，当与含有靶序列的核酸杂交时，组合物的荧光特性以可检测的方式变化。 还提供了合成方法和组合物的使用方法。