Hybridization-triggered fluorescent detection of nucleic acids
    2.
    发明申请
    Hybridization-triggered fluorescent detection of nucleic acids 失效
    杂交荧光检测核酸

    公开(公告)号:US20070166723A1

    公开(公告)日:2007-07-19

    申请号:US11242977

    申请日:2005-10-03

    IPC分类号: C12Q1/68 C07H21/04

    摘要: Compositions and methods for fluorescent detection of nucleic acids are provided. The compositions can be detected by fluorescence when hybridized to a nucleic acid containing a target sequence, but are non-fluorescent in the non-hybridized state. Alternatively, the fluorescence properties of the compositions change in a detectable manner upon hybridization to a nucleic acid containing a target sequence. Methods for synthesis and methods of use of the compositions are also provided.

    摘要翻译: 提供了核酸荧光检测的组合物和方法。 当与含有靶序列的核酸杂交时,可以通过荧光检测组合物,但是在非杂交状态下是非荧光的。 或者,当与含有靶序列的核酸杂交时,组合物的荧光特性以可检测的方式变化。 还提供了合成方法和组合物的使用方法。

    Systems and methods for isolating stromal cells from adipose tissue and uses thereof
    7.
    发明申请
    Systems and methods for isolating stromal cells from adipose tissue and uses thereof 审中-公开
    用于从脂肪组织分离基质细胞的系统和方法及其用途

    公开(公告)号:US20060051865A1

    公开(公告)日:2006-03-09

    申请号:US11217087

    申请日:2005-08-31

    IPC分类号: C12N5/08

    摘要: Methods of isolating cells from adipose tissue that have potential to differentiate into cells of mesenchymal origin, including cells of chondrogenic, osteogenic, adipogenic, and/or myogenic origin, comprising: (a) subjecting adipose tissue to an electromagnetic, sonic, or other wave energy source; and (b) centrifuging the tissue to form a pellet comprising stem cells. In various embodiments, the method is carried out without any enzymatic digestion of the adipose tissue. In other embodiments, the method additionally comprises enzymatically digesting the tissue. In various embodiments, methods comprise subjecting the tissue to ultrasonic energy. In some embodiments, the method does not comprise enzymatic digestion of the adipose tissue. In other embodiments, the method additionally comprises enzymatically digesting the tissue. Methods are also provided for intraoperative harvest and delivery of autologous stem cells to the site of acute or chronic wounds. In one embodiment, an autologous supply of stem cells is provided for operative use.

    摘要翻译: 从具有潜在分化成间充质来源的细胞的脂肪组织分离细胞的方法,包括软骨形成,成骨,脂肪形成和/或肌原性来源的细胞,包括:(a)使脂肪组织进行电磁,声波或其它波 能量源; 和(b)离心组织以形成包含干细胞的丸粒。 在各种实施方案中,该方法不进行任何酶消化的脂肪组织。 在其它实施方案中,该方法还包括酶促消化组织。 在各种实施例中,方法包括使组织经受超声能量。 在一些实施方案中,该方法不包括脂肪组织的酶消化。 在其它实施方案中,该方法还包括酶促消化组织。 还提供了用于术中收获和将自体干细胞递送至急性或慢性伤口部位的方法。 在一个实施例中,提供干细胞的自体供应用于操作使用。

    Methods and apparatus for promoting hair growth using adipose cell based therapies
    9.
    发明申请
    Methods and apparatus for promoting hair growth using adipose cell based therapies 审中-公开
    使用基于脂肪细胞的疗法促进头发生长的方法和装置

    公开(公告)号:US20070258956A1

    公开(公告)日:2007-11-08

    申请号:US11417302

    申请日:2006-05-02

    IPC分类号: A61K35/12 C12N5/08

    摘要: Methods and compositions for use in the treatment of androgenetic alopecia and other forms of hair loss by inducing and expediting the growth of hair in the scalp of a patient. Methods comprise the steps of isolating adipose-derived cells and hair follicles from the patient and implanting the combined cells and follicles into the scalp in areas requiring new growth of hair. Use of differentiated and undifferentiated adipose-derived cells with processed and grafted hair follicles for the therapeutic and cosmetic treatment of hair loss in vivo is disclosed.

    摘要翻译: 用于治疗雄激素性脱发和其它形式脱发的方法和组合物,其通过诱导和加速患者头皮中毛发的生长。 方法包括从患者中分离脂肪来源的细胞和毛囊并将合并的细胞和卵泡植入需要头发新生长的区域中的头皮中的步骤。 公开了分化和未分化脂肪来源的细胞与加工的和接种的毛囊用于体内脱发的治疗和美容治疗的用途。