摘要:
Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor groove binders and reporter groups.
摘要:
Compositions and methods for fluorescent detection of nucleic acids are provided. The compositions can be detected by fluorescence when hybridized to a nucleic acid containing a target sequence, but are non-fluorescent in the non-hybridized state. Alternatively, the fluorescence properties of the compositions change in a detectable manner upon hybridization to a nucleic acid containing a target sequence. Methods for synthesis and methods of use of the compositions are also provided.
摘要:
Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor groove binders and reporter groups.
摘要:
Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor groove binders and reporter groups.
摘要:
The invention provides compositions and methods for improved hybridization analysis utilizing DNA, RNA, PNA and chimeric oligomers in which one or more purine bases are substituted by a pyrazolo[5,4-d]pyrimidine or by a 7-deazapurine purine analogue. Reduced self-aggregation and reduced fluorescence quenching are obtained when the oligomers are used in various methods involving hybridization. Methods of synthesis, as well as novel synthetic precursors, are also provided.
摘要:
Oligonucleotide probes containing two labels are provided and are useful in hybridization assays. The probes can also contain a minor groove binding group.
摘要:
Methods of isolating cells from adipose tissue that have potential to differentiate into cells of mesenchymal origin, including cells of chondrogenic, osteogenic, adipogenic, and/or myogenic origin, comprising: (a) subjecting adipose tissue to an electromagnetic, sonic, or other wave energy source; and (b) centrifuging the tissue to form a pellet comprising stem cells. In various embodiments, the method is carried out without any enzymatic digestion of the adipose tissue. In other embodiments, the method additionally comprises enzymatically digesting the tissue. In various embodiments, methods comprise subjecting the tissue to ultrasonic energy. In some embodiments, the method does not comprise enzymatic digestion of the adipose tissue. In other embodiments, the method additionally comprises enzymatically digesting the tissue. Methods are also provided for intraoperative harvest and delivery of autologous stem cells to the site of acute or chronic wounds. In one embodiment, an autologous supply of stem cells is provided for operative use.
摘要:
Methods and compositions for use in the treatment of androgenetic alopecia and other forms of hair loss by inducing and expediting the growth of hair in the scalp of a patient. Methods comprise the steps of isolating adipose-derived cells and hair follicles from the patient and implanting the combined cells and follicles into the scalp in areas requiring new growth of hair. Use of differentiated and undifferentiated adipose-derived cells with processed and grafted hair follicles for the therapeutic and cosmetic treatment of hair loss in vivo is disclosed.