Methods for quantifying polypeptides using mass spectrometry
    5.
    发明授权
    Methods for quantifying polypeptides using mass spectrometry 有权
    使用质谱法定量多肽的方法

    公开(公告)号:US09588130B2

    公开(公告)日:2017-03-07

    申请号:US14394952

    申请日:2013-03-14

    Abstract: A method for identifying a polypeptide a specimen can include (i) treating a specimen suspected of including an insulin with a base; (ii) extracting a first fraction of the treated specimen by solid phase extraction using a mixed mode or polymeric reversed-phase media and a first solvent including an acid; (iii) separating a component of the first fraction by liquid chromatography using a chromatographic surface including a hydrophobic surface group and one or more ionizable modifiers, and a second solvent including an acid; and (iv) analyzing the component of the first fraction by mass spectroscopy, thereby identifying the polypeptide, if present, using a signal corresponding to a sequence fragment ion from the polypeptide. The signal can correspond to an intact multiply charged precursor fragment selected in a first quadrupole and its corresponding sequence fragment ion selected in a final quadrupole.

    Abstract translation: 用于鉴定样品的多肽的方法可以包括(i)将疑似包含胰岛素的样品用碱处理; (ii)使用混合模式或聚合物反相介质和包含酸的第一溶剂通过固相萃取提取处理过的样品的第一级分; (iii)使用包括疏水表面基团和一种或多种可电离改性剂的色谱表面和包含酸的第二溶剂通过液相色谱分离第一级分的组分; 和(iv)通过质谱法分析第一级分的组分,从而使用对应于来自多肽的序列片段离子的信号鉴定多肽(如果存在)。 该信号可以对应于在第一个四极中选择的完整的多电荷前体片段及其在最终四极中选择的相应的序列片段离子。

    Methods for quantifying polypeptides using mass spectrometry

    公开(公告)号:US10241117B2

    公开(公告)日:2019-03-26

    申请号:US15965613

    申请日:2018-04-27

    Abstract: A method for identifying a polypeptide a specimen can include (i) treating a specimen suspected of including an insulin with a base; (ii) extracting a first fraction of the treated specimen by solid phase extraction using a mixed mode or polymeric reversed-phase media and a first solvent including an acid; (iii) separating a component of the first fraction by liquid chromatography using a chromatographic surface including a hydrophobic surface group and one or more ionizable modifiers, and a second solvent including an acid; and (iv) analyzing the component of the first fraction by mass spectroscopy, thereby identifying the polypeptide, if present, using a signal corresponding to a sequence fragment ion from the polypeptide. The signal can correspond to an intact multiply charged precursor fragment selected in a first quadrupole and its corresponding sequence fragment ion selected in a final quadrupole.

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