CROSS-LINKED GUMS FOR HEPATOCYTE CULTURE
    5.
    发明申请
    CROSS-LINKED GUMS FOR HEPATOCYTE CULTURE 审中-公开
    HEPATOCYTE文化的交叉关系

    公开(公告)号:US20100190255A1

    公开(公告)日:2010-07-29

    申请号:US12500088

    申请日:2009-07-09

    IPC分类号: C12N5/071

    摘要: This disclosure relates to cell culture surfaces derived from or contain gums including naturally occurring gums, plant gums, galactomannan gums or derivatives thereof including carboxyalkyl guar gum. Even more particularly, the disclosure relates to chemically or physically cross-linked modified gums where the gum surfaces are tuned to provide cell culture surfaces with physical and chemical characteristics particularly suited for hepatocyte culture. The disclosure also relates to articles of manufacture (e.g., cell culture vessels and labware) having such matrices, methods of making and providing the matrices to cell culture surfaces, and methods of using cell culture vessels having such matrices.

    摘要翻译: 本公开涉及源自或含有牙龈的细胞培养物表面,包括天然存在的树胶,植物胶,半乳甘露聚糖胶或其衍生物,包括羧烷基瓜耳胶。 更具体地,本公开涉及化学或物理交联的改性胶,其中胶表面被调谐以提供特别适合于肝细胞培养的物理和化学特性的细胞培养物表面。 本公开还涉及具有这种基质的制品(例如,细胞培养容器和实验器皿),制备和提供基质到细胞培养物表面的方法,以及使用具有这种基质的细胞培养容器的方法。

    LAMININ-ENTACTIN COMPLEX AND CELL CULTURE ARTICLE AND METHODS THEREOF
    7.
    发明申请
    LAMININ-ENTACTIN COMPLEX AND CELL CULTURE ARTICLE AND METHODS THEREOF 有权
    LAMININ-ENTACTIN复合物和细胞培养物品及其方法

    公开(公告)号:US20110306136A1

    公开(公告)日:2011-12-15

    申请号:US13111234

    申请日:2011-05-19

    IPC分类号: C12N5/071 C12M3/00

    摘要: A cell culture system including: a substrate, a substrate coating, one or more live cells, and an overlay source. The substrate coating and overlay can be laminin, and laminin•entactin complex, respectively. Alternatively, the substrate coating and overlay can be laminin•entactin complex, and laminin, respectively. The cell culture system can further include liquid media and a protective cover. A method for making and using the system in cell culture articles and culture methods, as defined herein, is also disclosed.

    摘要翻译: 一种细胞培养系统,包括:底物,底物涂层,一个或多个活细胞和覆盖源。 底物涂层和覆盖层可分别为层粘连蛋白和层粘连蛋白•内酰胺酶复合物。 或者,基底涂层和覆盖层可以分别是层粘连蛋白·内含子复合物和层粘连蛋白。 细胞培养系统还可以包括液体介质和保护盖。 还公开了在本文定义的细胞培养物和培养方法中制备和使用该系统的方法。

    Laminin-entactin complex and cell culture article and methods thereof
    10.
    发明授权
    Laminin-entactin complex and cell culture article and methods thereof 有权
    层粘蛋白 - 内含蛋白复合物和细胞培养物及其方法

    公开(公告)号:US08513015B2

    公开(公告)日:2013-08-20

    申请号:US13111234

    申请日:2011-05-19

    IPC分类号: C12N5/00

    摘要: A cell culture system including: a substrate, a substrate coating, one or more live cells, and an overlay source. The substrate coating and overlay can be laminin, and laminin•entactin complex, respectively. Alternatively, the substrate coating and overlay can be laminin•entactin complex, and laminin, respectively. The cell culture system can further include liquid media and a protective cover. A method for making and using the system in cell culture articles and culture methods, as defined herein, is also disclosed.

    摘要翻译: 一种细胞培养系统,包括:底物,底物涂层,一个或多个活细胞和覆盖源。 基底涂层和覆盖层可分别为层粘连蛋白和层粘连蛋白复合物。 或者,基底涂层和覆盖层可以分别是层粘连蛋白复合物和层粘连蛋白。 细胞培养系统还可以包括液体介质和保护盖。 还公开了在本文定义的细胞培养物和培养方法中制备和使用该系统的方法。