公开(公告)号：US06627159B1

公开(公告)日：2003-09-30

申请号：US09710184

申请日：2000-11-10

申请人： William Bedingham ,  Joel R. Dufresne ,  Michael R. Harms ,  Richard R. Matner ,  Diane North ,  Kenneth B. Wood

发明人： William Bedingham ,  Joel R. Dufresne ,  Michael R. Harms ,  Richard R. Matner ,  Diane North ,  Kenneth B. Wood

IPC分类号： B01L300

CPC分类号： G01N35/00069 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/502707 ,  B01L3/502738 ,  B01L7/52 ,  B01L7/525 ,  B01L9/527 ,  B01L2200/0642 ,  B01L2200/0689 ,  B01L2200/142 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2400/0406 ,  B01L2400/0409 ,  B01L2400/0481 ,  B01L2400/0655 ,  B01L2400/0661 ,  F27B9/16 ,  F27D5/00 ,  Y10T436/25 ,  Y10T436/2575

摘要： The present invention provides methods and devices for distributing sample material to a plurality of process chambers in a sample processing device by rotating the device about an axis of rotation. The process chambers are located along conduits extending from a loading chamber and, together, the loading chamber, conduits, and process chambers form process arrays that are aligned along a length of the sample processing devices. The process arrays are unvented, i.e., access to the interior volume of the process arrays is available only through the loading chamber. Also disclosed are methods of centrifugally loading sample material into the process chambers, as well as an assembly including a sample processing device and a carrier.

摘要翻译： 本发明提供了通过围绕旋转轴旋转设备来将样品材料分配到样品处理装置中的多个处理室的方法和装置。 处理室沿着从加载室延伸的管道定位，并且一起，装载室，导管和处理室形成沿着样品处理装置的长度对准的工艺阵列。 过程阵列是不受影响的，即，只能通过装载室访问过程阵列的内部空间。 还公开了将样品材料离心加载到处理室中的方法，以及包括样品处理装置和载体的组件。

公开(公告)号：US5418167A

公开(公告)日：1995-05-23

申请号：US106816

申请日：1993-08-13

申请人： Richard R. Matner ,  William E. Foltz ,  Lewis P. Woodson

发明人： Richard R. Matner ,  William E. Foltz ,  Lewis P. Woodson

IPC分类号： C12Q1/22 ,  C12M1/40 ,  C12Q1/34 ,  C12Q1/42

CPC分类号： C12Q1/22 ,  Y10S435/81

摘要： A rapid method of determining the efficacy of a sterilization cycle, and an indicator adapted to perform such method, comprising subjecting to the sterilization cycle a source of active enzyme having activity which correlates with the viability of a microorganism commonly used to monitor sterilization, and incubating the enzyme source, following the completion of the sterilization cycle, with an effective amount of a substrate system capable of reacting with any residual active enzyme to produce a detectable enzyme-modified product.

公开(公告)号：US06623955B2

公开(公告)日：2003-09-23

申请号：US08443820

申请日：1995-05-18

申请人： Richard R. Matner ,  William E. Foltz ,  Lewis P. Woodson

发明人： Richard R. Matner ,  William E. Foltz ,  Lewis P. Woodson

IPC分类号： C12M300

CPC分类号： C12Q1/22 ,  Y10S435/81

摘要： A rapid method of determining the efficacy of a sterilization cycle, and an indicator adapted to perform such method, comprising subjecting to the sterilization cycle a source of active enzyme having activity which correlates with the viability of a microorganism commonly used to monitor sterilization, and incubating the enzyme source, following the completion of the sterilization cycle, with an effective amount of a substrate system capable of reacting with any residual active enzyme to produce a detectable enzyme-modified product.

摘要翻译： 确定灭菌循环功效的快速方法，以及适于进行这种方法的指示剂，包括对灭菌周期进行活性酶源，该酶活性与通常用于监测灭菌的微生物的活力相关，并且孵育 在灭菌周期完成之后，酶源与有效量的能够与任何残留活性酶反应以产生可检测酶修饰产物的底物系统。

公开(公告)号：US5137812A

公开(公告)日：1992-08-11

申请号：US742270

申请日：1991-08-07

申请人： Richard R. Matner

发明人： Richard R. Matner

IPC分类号： C12Q1/04 ,  G01N33/569

CPC分类号： C12Q1/04 ,  G01N33/56916 ,  G01N2333/245 ,  G01N2400/50

摘要： A method and article for the identification of colonies of microbes involves the use of a reconstitutable dry culture medium plate in combination with a transfer membrane. Microbe-specific antigen can be extracted from the surface of the growth medium of the plate and its presence determined, e.g., by immunoassay.

公开(公告)号：US5073488A

公开(公告)日：1991-12-17

申请号：US277305

申请日：1988-11-29

申请人： Richard R. Matner ,  William E. Foltz ,  Lewis P. Woodson

发明人： Richard R. Matner ,  William E. Foltz ,  Lewis P. Woodson

IPC分类号： A61L2/26 ,  C12Q1/22 ,  C12Q1/25

CPC分类号： C12Q1/22

摘要： A rapid method of determining the efficacy of a sterilization cycle, and an indicator adapted to perform such method, comprising subjecting to the sterilization cycle a source of active enzyme having activity which correlates with the viability of a microorganism commonly used to monitor sterilization, and incubating the enzyme source, following the completion of the sterilization cycle, with an effective amount of a substrate system capable of reacting with any residual active enzyme to produce a detectable enzyme-modified product.

公开(公告)号：US5252484A

公开(公告)日：1993-10-12

申请号：US748327

申请日：1991-08-21

申请人： Richard R. Matner ,  William E. Foltz ,  Lewis P. Woodson

发明人： Richard R. Matner ,  William E. Foltz ,  Lewis P. Woodson

IPC分类号： C12Q1/22 ,  C12M1/00 ,  C12M1/34

CPC分类号： C12Q1/22 ,  Y10S435/81

摘要： A rapid method of determining the efficacy of a sterilization cycle, and an indicator adapted to perform such method, comprising subjecting to the sterilization cycle a source of active enzyme having activity which correlates with the viability of a microorganism commonly used to monitor sterilization, and incubating the enzyme source, following the completion of the sterilization cycle, with an effective amount of a substrate system capable of reacting with any residual active enzyme to produce a detectable enzyme-modified product.

摘要翻译： 确定灭菌循环功效的快速方法，以及适于进行这种方法的指示剂，包括对灭菌周期进行活性酶源，该酶活性与通常用于监测灭菌的微生物的活力相关，并且孵育 在灭菌周期完成之后，酶源与有效量的能够与任何残留活性酶反应以产生可检测酶修饰产物的底物系统。

公开(公告)号：US5223401A

公开(公告)日：1993-06-29

申请号：US277570

申请日：1988-11-29

申请人： William E. Foltz ,  Richard R. Matner ,  Lewis P. Woodson

发明人： William E. Foltz ,  Richard R. Matner ,  Lewis P. Woodson

IPC分类号： C12Q1/22

CPC分类号： C12Q1/22 ,  Y10S435/808 ,  Y10S435/81 ,  Y10S436/805

摘要： A unitary sterility indicator and a method for its use, the indicator comprising an outer container having liquid impermeable and gas non-absorptive walls, and having a gas-transmissive, bacteria impermeable opening therein; contained within the outer container, a detectable amount of a source of active enzyme and/or another microorganism commonly used to monitor sterilization; a sealed, openable gas and liquid impermeable inner container containing an aqueous medium and/or a nutrient growth medium, disposed in the outer container; an enzyme substrate system capable of reacting with active enzyme to produce a detectable enzyme-modified product and/or a detector material sensitive to microorganism growth, contained in one of the containers; and means contained within the outer container for restricting the area in which enzyme-modified product and/or growing microorganisms are contained, after the inner container is opened, to an area which is less than the volume of aqueous solution contained in the inner container.

摘要翻译： 一种单一的无菌指示剂及其使用方法，该指示剂包括具有不透液体和气体不吸收壁的外部容器，并且其中具有气体透过的细菌不可渗透的开口; 包含在外容器内的可检测量的活性酶源和/或通常用于监测灭菌的另一种微生物来源; 包含设置在外部容器中的含有水性介质和/或营养生长介质的密封且可开启的气体和液体不可渗透的内部容器; 能够与活性酶反应以产生可检测的酶修饰产物和/或容纳在其中一个容器中的对微生物生长敏感的检测器材料的酶底物系统; 以及包含在外容器内的装置，用于将内容器打开之后将酶改性产物和/或生长微生物包含的区域限制在小于内容器中所含的水溶液体积的区域。