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公开(公告)号：US4343736A
公开(公告)日：1982-08-10
申请号：US280687
申请日：1981-07-06
IPC分类号： C12P21/00 , A61K38/21 , C07K1/22 , C07K14/52 , C07K14/555 , C07K14/56 , C07K14/57 , A61K45/02 , C07G7/00
CPC分类号： C07K14/555 , Y10S435/811 , Y10S530/829 , Y10S530/837 , Y10S530/838 , Y10S530/848
摘要： A process for recovering interferon which comprises contacting a solution containing an interferon produced by the induced cells of human origin with a water-insolubilized sulfated polysaccharide to allow the interferon to be adsorbed on the water-insolubilized sulfated polysaccharide and then selectively eluting the interferon with an aqueous solution of an inorganic salt.
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公开(公告)号：US4526782A
公开(公告)日：1985-07-02
申请号：US432909
申请日：1982-09-27
CPC分类号： C07K14/56
摘要： Interferon can be recovered simply by contacting an aqueous solution containing interferon which is induced and produced from cells of human origin capable of producing interferon, with a siliceous substance to adsorb interferon on it and eluting the adsorbed interferon with an aqueous solution containing a nonionic surfactant, preferably further containing an acriflavine such as acrinol.
摘要翻译： PCT No.PCT / JP81 / 00030 Sec。 371日期1982年9月27日 102（e）日期1982年9月27日PCT提交1981年2月17日PCT公布。 出版物WO82 / 02834 日本1982年9月2日。通过将含有可产生干扰素的人源细胞诱导和产生的含有干扰素的水溶液与硅质物质接触，可以简单地回收地塞米松，以吸附干扰素并用吸附的干扰素 含有非离子表面活性剂的水溶液，优选还含有吖啶如Acrinol。
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公开(公告)号：US4314935A
公开(公告)日：1982-02-09
申请号：US175744
申请日：1980-08-06
IPC分类号： C12P21/00 , A61K38/21 , C07K1/22 , C07K14/52 , C07K14/555 , C07K14/56 , C07K14/57 , A61K45/02 , C07G7/00
CPC分类号： C07K14/555 , Y10S435/811 , Y10S530/829 , Y10S530/837 , Y10S530/838 , Y10S530/848
摘要： A process for recovering interferon which comprises contacting a solution containing an interferon produced by the induced cells of human origin with a water-insolubilized sulfated polysaccharide to allow the interferon to be adsorbed on the water-insolubilized sulfated polysaccharide and then selectively eluting the interferon with an aqueous solution of an inorganic salt.
摘要翻译： 一种用于回收干扰素的方法，其包括将含有由人源诱导的细胞产生的干扰素的溶液与水不溶性硫酸化多糖接触以允许干扰素被吸附在水不溶性硫酸化多糖上，然后选择性地洗脱干扰素 无机盐水溶液。
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公开(公告)号：US4694074A
公开(公告)日：1987-09-15
申请号：US792081
申请日：1985-10-28
申请人： Yahiro Uemura , Takao Ohmura , Akimasa Ohmizu , Akinori Sumi , Wataru Ohtani , Yoshitaka Sakanishi , Hiroshi Morise , Hirofumi Arimura , Tadakazu Suyama
发明人： Yahiro Uemura , Takao Ohmura , Akimasa Ohmizu , Akinori Sumi , Wataru Ohtani , Yoshitaka Sakanishi , Hiroshi Morise , Hirofumi Arimura , Tadakazu Suyama
IPC分类号： C12N15/09 , A61K39/29 , A61P1/16 , C07K1/16 , C07K1/22 , C07K14/005 , C07K14/02 , C07K14/195 , C07K14/435 , C12N5/00 , C12N15/00 , C12P21/00 , C12P21/08 , C07K3/18 , C07K3/28
CPC分类号： C07K14/005 , C12N2730/10122 , Y10S530/806 , Y10S530/808
摘要： A process for the purification of HBsAg is disclosed, which comprises adsorbing specifically on a carrier, in the presence of an inorganic salt in an amount of 5 to 25 W/V %, an HBsAg obtained by gene engineering.
摘要翻译： 公开了一种用于纯化HBsAg的方法，其包括在载体上以5至25W / V％的无机盐存在下特异性吸附通过基因工程获得的HBsAg。
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公开(公告)号：US4945046A
公开(公告)日：1990-07-31
申请号：US57143
申请日：1987-06-03
申请人： Hajime Horii , Haruhide Kawabe , Hirofumi Arimura , Hiromichi Mukai , Kaoru Kobayashi , Muneo Tsujikawa , Masayuki Nishida , Tadakazu Suyama
发明人： Hajime Horii , Haruhide Kawabe , Hirofumi Arimura , Hiromichi Mukai , Kaoru Kobayashi , Muneo Tsujikawa , Masayuki Nishida , Tadakazu Suyama
CPC分类号： C07K14/005 , C12N15/81 , C12N2730/10122
摘要： A method of producing a heterologous protein in yeast is disclosed, which comprises transforming a yeast Saccharomyces cerevisiae with recombinant DNA comprising a promoter selected from the group consisting of a yeast promoter or a hybrid promoter derived from a yeast promoter, particularly a hybrid promoter containing the enhancer region of SV40 virus, and a gene coding for a heterologous protein such as HBsAg and Pre S-HBsAg, particularly full length Pre S-HBsAg culturing, the resulting transformed cells to express the gene coding the heterologous protein, and isolating the heterologous protein from the cultured medium. GAP-DH and PH05 promoters and miniaturized ones can be used as the yeast promoter. The expression of the full length Pre S-HBsAg is carried out in the same expression system as that for 2nd or 3rd Pre S-ABsAg.
摘要翻译： 公开了一种在酵母中产生异源蛋白质的方法，其包括用包含启动子的重组DNA转化酵母酿酒酵母，所述启动子选自酵母启动子或源自酵母启动子的杂合启动子，特别是含有 SV40病毒的增强子区和编码异源蛋白如HBsAg和Pre S-HBsAg的基因，特别是全长Pre S-HBsAg培养，所得转化细胞表达编码异源蛋白的基因，并分离异源蛋白 从培养基中。 GAP-DH和PH05启动子和小型化可用作酵母启动子。 全长前S-HBsAg的表达在与第2或第3前S-ABsAg相同的表达系统中进行。
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公开(公告)号：US4313938A
公开(公告)日：1982-02-02
申请号：US58361
申请日：1979-07-18
CPC分类号： C07H21/00 , C12N9/1247 , C12P19/34 , Y10S435/815 , Y10S435/816 , Y10S435/948 , Y10S514/889
摘要： Double-stranded RNA synthesized using native human DNA as template is found to be an excellent interferon inducer with low toxicity in spite of homologous native to host cell. It is produced by reacting ATP, GTP, CTP and UTP with one another in the presence of a native human DNA as template by the catalytic action of an active RNA polymerase to form RNA and subjecting the resulting RNA to annealing by heating it at a temperature of 70.degree. to 100.degree. C. and gradually cooling to room temperature or below to form double-stranded regions between their molecules.
摘要翻译： 使用天然人DNA作为模板合成的双链RNA被发现是优良的干扰素诱导物，尽管与宿主细胞天然存在同源性，但毒性低。 通过活性RNA聚合酶的催化作用，在天然人DNA作为模板的存在下，通过使ATP，GTP，CTP和UTP彼此反应来生成RNA，并通过在温度下加热使得到的RNA进行退火 70℃〜100℃，逐渐冷却至室温以下，形成分子间的双链区域。
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公开(公告)号：US5021339A
公开(公告)日：1991-06-04
申请号：US397347
申请日：1989-08-24
申请人： Hiromichi Mukai , Hajime Horii , Muneo Tsujikawa , Haruhide Kawabe , Hirofumi Arimura , Tadakazu Suyama
发明人： Hiromichi Mukai , Hajime Horii , Muneo Tsujikawa , Haruhide Kawabe , Hirofumi Arimura , Tadakazu Suyama
CPC分类号： C07K14/005 , C12N15/81 , C12N2730/10122
摘要： A DNA sequence of the yeast glyceraldehyde-3-phosphate dehydrogenase (GAP-DH) promoter and a process for preparing a heterologous protein utilizing said DNA sequence as a promoter are disclosed, said GAP-DH promoter comprising a region upstream of an initiator codon of the GAP-DH protein up to -164 bp as a minimum unit. Since the GAP-DH promoter is small in size, a physiologically active substance can be expressed in yeasts effectively and recombination of DNA can be simplified.
摘要翻译： 公开了酵母甘油醛-3-磷酸脱氢酶（GAP-DH）启动子的DNA序列和利用所述DNA序列作为启动子制备异源蛋白质的方法，所述GAP-DH启动子包含上游的起始密码子 最高达-164 bp的GAP-DH蛋白作为最小单位。 由于GAP-DH启动子的大小小，所以能够有效地在酵母中表达生理活性物质，可以简化DNA的重组。
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公开(公告)号：US4757021A
公开(公告)日：1988-07-12
申请号：US795144
申请日：1985-11-05
申请人： Haruhide Kawabe , Kazumoto Hirabayashi , Hazime Horii , Hirofumi Arimura , Masayuki Nishida , Tadakazu Suyama
发明人： Haruhide Kawabe , Kazumoto Hirabayashi , Hazime Horii , Hirofumi Arimura , Masayuki Nishida , Tadakazu Suyama
IPC分类号： C12N15/09 , C07K14/02 , C12N1/16 , C12N15/68 , C12N15/81 , C12P21/00 , C12R1/865 , C12N1/18 , C12N1/00 , C12N15/00
CPC分类号： C07K14/005 , C12N15/68 , C12N15/81 , C12N2730/10122
摘要： A recombinant plasmid wherein at least two DNA fragments containing a Baker gene and a Charlie gene of the yeast 2 .mu.m circular plasmid are incorporated into a plasmid containing genes encoding a physiologically active substance is disclosed. Also, a strain transformed by the recombinant plasmid is disclosed.
摘要翻译： 公开了将包含酵母2μm圆形质粒的Baker基因和Charlie基因的至少两个DNA片段并入包含编码生理活性物质的基因的质粒中的重组质粒。 此外，公开了由重组质粒转化的菌株。
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公开(公告)号：US4555401A
公开(公告)日：1985-11-26
申请号：US589863
申请日：1984-03-19
申请人： Hirofumi Arimura , Yahiro Uemura , Yoshiomi Okuno
发明人： Hirofumi Arimura , Yahiro Uemura , Yoshiomi Okuno
IPC分类号： A61K39/165 , A61K47/42
CPC分类号： A61K47/42 , A61K39/12 , A61K39/165 , A61K2039/5254 , C12N2760/18734
摘要： A live mumps vaccine can be stabilized to a great extent by adding in an amount, effective for stabilizing said vaccine, at least one stabilizer selected from the group consisting of albumin and gelatin.
摘要翻译： 活腮腺炎疫苗可以在很大程度上通过加入有效稳定所述疫苗，至少一种选自白蛋白和明胶的稳定剂的量来稳定。
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公开(公告)号：US5137824A
公开(公告)日：1992-08-11
申请号：US168016
申请日：1988-03-14
CPC分类号： C12N9/6462 , C07K14/53 , C12N15/85 , C12Y304/21073
摘要： A CSF-1 gene promoter region is disclosed. This nucleotide base sequence is useful as a promoter for gene expression in animal cells.
摘要翻译： 公开了CSF-1基因启动子区。 该核苷酸碱基序列可用作动物细胞中基因表达的启动子。
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