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    Shuttle vector for Bifidobacterium and Escherichia coli
    1.
    发明授权
    Shuttle vector for Bifidobacterium and Escherichia coli 有权
    穿梭载体为双歧杆菌和大肠杆菌

    公开(公告)号：US08021653B2

    公开(公告)日：2011-09-20

    申请号：US11718680

    申请日：2005-11-24

    申请人： Yasunobu Kano ,  Yoshinori Hamaji ,  Minoru Fujimori ,  Takayuki Sasaki ,  Kyoko Kohno ,  Jun Amano ,  Shun'ichiro Taniguchi

    发明人： Yasunobu Kano ,  Yoshinori Hamaji ,  Minoru Fujimori ,  Takayuki Sasaki ,  Kyoko Kohno ,  Jun Amano ,  Shun'ichiro Taniguchi

    IPC分类号： C07H21/04 ,  C12N15/00 ,  C12N1/21

    CPC分类号： C12N15/70 ,  A61K38/00 ,  C12N15/746

    摘要： The present invention provides a shuttle vector for a microorganism of the genus Bifidobacterium (BM) and Escherichia coli having a wide host range and a large copy number in BM and capable of highly expressing a desired protein when used as an expression vector; an expression vector capable of expressing a desired gene in BM by use of the shuttle vector; BM transformed with the expression vector; and an antitumor agent comprising the BM as an active ingredient. It comprises a pTB6-derived region portion comprising a replication origin (oriV)-repB region of pTB6 but not comprising MembB, MobA, OrfI, and oriT regions of pTB6 and an Escherichia coli-derived plasmid portion comprising a replication origin (Puc Ori) region of Escherichia coli but having deleted DNA encoding an N-terminal region of an ampicillin resistance gene (ampR) expression product β-lactamase.

    摘要翻译： 本发明提供了一种用于双歧杆菌（BM）和微生物的穿梭载体，其具有宽的宿主范围，并且在BM中具有大的拷贝数，并且当用作表达载体时能够高度表达所需的蛋白质。 能够通过穿梭载体在BM中表达所需基因的表达载体; BM用表达载体转化; 和包含BM作为活性成分的抗肿瘤剂。 其包含pTB6衍生的区域部分，其包含pTB6的复制起点（oriV）-repB区，但不包含pBB6的MembB，MobA，OrfI和oriT区以及包含复制起点（Puc Ori）的大肠杆菌来源的质粒部分， 大肠杆菌的区域，但是具有编码氨苄青霉素抗性基因（ampR）表达产物＆amp;内酰胺酶的N末端区域的缺失的DNA。

    Novel Shuttle Vector
    2.
    发明申请
    Novel Shuttle Vector 有权
    小号班车矢量

    公开(公告)号：US20100129323A1

    公开(公告)日：2010-05-27

    申请号：US11718680

    申请日：2005-11-24

    申请人： Yasunobu Kano ,  Yoshinori Hamaji ,  Minoru Fujimori ,  Takayuki Sasaki ,  Kyoko Kohno ,  Jun Amano ,  Shun'ichiro Taniguchi

    发明人： Yasunobu Kano ,  Yoshinori Hamaji ,  Minoru Fujimori ,  Takayuki Sasaki ,  Kyoko Kohno ,  Jun Amano ,  Shun'ichiro Taniguchi

    IPC分类号： A61K35/00 ,  A61P43/00 ,  C12N1/20 ,  C12N15/00

    CPC分类号： C12N15/70 ,  A61K38/00 ,  C12N15/746

    摘要： The present invention provides a shuttle vector for a microorganism of the genus Bifidobacterium (BM) and Escherichia coli having a wide host range and a large copy number in BM and capable of highly expressing a desired protein when used as an expression vector; an expression vector capable of expressing a desired gene in BM by use of the shuttle vector; BM transformed with the expression vector; and an antitumor agent comprising the BM as an active ingredient. It comprises a pTB6-derived region portion comprising a replication origin (oriV)-repB region of pTB6 but not comprising MembB, MobA, OrfI, and oriT regions of pTB6 and an Escherichia coli-derived plasmid portion comprising a replication origin (oriC) region of Escherichia coli but having deleted DNA encoding an N-terminal region of an ampicillin resistance gene (ampR) expression product β-lactamase, and constitute a shuttle vector for Escherichia coli with a BM having an average copy number of 6 to 30.

    摘要翻译： 本发明提供了一种用于双歧杆菌（BM）和微生物的穿梭载体，其具有宽的宿主范围，并且在BM中具有大的拷贝数，并且当用作表达载体时能够高度表达所需的蛋白质。 能够通过穿梭载体在BM中表达所需基因的表达载体; BM用表达载体转化; 以及含有BM作为活性成分的抗肿瘤剂。 其包含pTB6衍生的区域部分，其包含pTB6的复制起点（oriV）-repB区，但不包含pBB6的MembB，MobA，OrfI和oriT区以及包含复制起点（oriC）区的大肠杆菌来源的质粒部分 的大肠杆菌，但是具有编码氨苄青霉素抗性基因（ampR）表达产物和β-内酰胺酶的N末端区域的缺失的DNA，并且构建具有平均拷贝数为6至30的BM的大肠杆菌的穿梭载体。