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    Method of measuring biological ligand
    1.
    发明授权
    Method of measuring biological ligand 失效
    生物配体测定方法

    公开(公告)号：US4649105A

    公开(公告)日：1987-03-10

    申请号：US603499

    申请日：1984-04-24

    申请人： Yasushi Kasahara ,  Yoshihiro Ashihara ,  Hiromasa Suzuki

    发明人： Yasushi Kasahara ,  Yoshihiro Ashihara ,  Hiromasa Suzuki

    IPC分类号： G01N33/53 ,  G01N33/542 ,  G01N33/543 ,  G01N33/545 ,  G01N33/58 ,  G01N33/569 ,  G01N33/574

    CPC分类号： G01N33/545 ,  G01N33/54306 ,  G01N33/581 ,  Y10S436/813 ,  Y10S436/817

    摘要： A method of measuring a biological ligand comprises allowing to coexist a biologically active composition comprising an immobilization phase of a particular antibody or a particular ligand and an immobilization phase of a biotinyl enzyme or a biotinyl enzyme inhibitor, a water-soluble conjugate of the ligand or the antibody and the biotinyl enzyme inhibitor or the biotinyl enzyme, and the ligand to be measured in an aqueous solution, and measuring the remaining biotinyl enzyme activity or the biotinyl enzyme inhibitory activity. This method is highly sensitive and specific, and it is suitable as a clinical test for the determination of physiological substances and trace components in humoral fluid.

    摘要翻译： 测量生物配体的方法包括允许共存包含特定抗体或特定配体的固定相的生物活性组合物和生物素酶或生物素酶抑制剂的固定相，配体的水溶性缀合物或 抗体和生物素抑制剂或生物素酶，以及在水溶液中待测量的配体，并测量剩余的生物素酶活性或生物素酶抑制活性。 该方法灵敏度高，具有特异性，适合作为体液中生理物质和微量成分测定的临床试验。

    Method for determining the activity of the angiotensin-converting enzyme
    5.
    发明授权
    Method for determining the activity of the angiotensin-converting enzyme 失效
    测定血管紧张素转换酶活性的方法

    公开(公告)号：US4407944A

    公开(公告)日：1983-10-04

    申请号：US330110

    申请日：1981-12-14

    申请人： Yasushi Kasahara ,  Yoshihiro Ashihara

    发明人： Yasushi Kasahara ,  Yoshihiro Ashihara

    IPC分类号： G01N33/50 ,  C12Q1/34 ,  C12Q1/37 ,  C12Q1/36

    CPC分类号： C12Q1/37 ,  C12Q1/34 ,  C12Q2326/96 ,  C12Q2337/00

    摘要： A method for determining the activity of an angiotensin-converting enzyme is disclosed comprising the steps of mixing a liquid containing (i) a synthetic substrate X-hippuryl-L-dipeptide having the formula ##STR1## wherein X represents OH, NH.sub.2 or N(CH.sub.3).sub.2, and when R.sub.1 is H, R.sub.2 is H, and when R.sub.1 is ##STR2## R.sub.2 is CH.sub.2 CH(CH.sub.3).sub.2, (ii) hippuricase and (iii) 4-aminoantipyrine, with a liquid containing an angiotensin-converting enzyme; measuring colorimetrically the concentration of the quinonimine dye which is formed by adding an oxiding agent to the above mixture; and calculating the activity of the angiotensin-converting enzyme from the concentration of the quinonimine dye.

    摘要翻译： 公开了一种用于测定血管紧张素转换酶的活性的方法，包括以下步骤：混合含有（i）具有式“IMAGE”的合成底物X-马尿基-L-二肽的液体，其中X表示OH，NH 2或N（ CH 3）2，当R 1为H时，R 2为H，当R 1为CH 2 CH（CH 3）2时，（ⅱ）马尿酸和（iii）4-氨基安替比林与含有血管紧张素转化酶的液体 ; 通过向上述混合物中加入氧化剂形成的醌亚胺染料的浓度进行比色测定; 并从醌亚胺染料的浓度计算血管紧张素转换酶的活性。

    Method of measuring biological ligand by utilizing amylase
    6.
    发明授权
    Method of measuring biological ligand by utilizing amylase 失效
    利用淀粉酶测定生物配体的方法

    公开(公告)号：US4757001A

    公开(公告)日：1988-07-12

    申请号：US699675

    申请日：1985-02-08

    申请人： Yoshihiro Ashihara ,  Yasushi Kasahara

    发明人： Yoshihiro Ashihara ,  Yasushi Kasahara

    IPC分类号： G01N33/542 ,  G01N33/563

    CPC分类号： G01N33/563 ,  G01N33/542

    摘要： The present invention relates to the method of measuring a biological ligand (1) in a sample containing an amylase (1) derived from a higher animal. This method is characterized by employing a combination of an amylase (2) not contained in the sample and an antibody against the ligand (1) or a ligand (2) capable of reacting with the antibody and an amylase inhibitor of which inhibitory activity against the amylase (1) is stronger than that against the amylase (2). The method of the invention utilizes the emphasis of steric hindrance of the amylase (2) reaction which is carried out at the boundary between solid phase and liquid phase and the specific removal of the influence of the amylase (1) by using the above amylase inhibitor. This method is suitable as a clinical test for the determination of physiological substances and trace components in a humoral fluid such as serum.

    摘要翻译： 本发明涉及含有来自较高级动物的淀粉酶（1）的样品中的生物配体（1）的测定方法。 该方法的特征在于使用样品中不含有的淀粉酶（2）和配体（1）的抗体或能够与抗体反应的配体（2）和对该抗体具有抑制活性的淀粉酶抑制剂的组合 淀粉酶（1）比对淀粉酶（2）更强。 本发明的方法利用在固相和液相边界处进行的淀粉酶（2）反应的空间位阻的强调，以及通过使用上述淀粉酶抑制剂特异性去除淀粉酶（1）的影响 。 该方法适用于测定体液如血清中生理物质和痕量成分的临床试验。

    Method of measuring biological ligand by the use of enzymes
    7.
    发明授权
    Method of measuring biological ligand by the use of enzymes 失效
    通过使用酶测量生物配体的方法

    公开(公告)号：US4692404A

    公开(公告)日：1987-09-08

    申请号：US670764

    申请日：1984-11-13

    申请人： Yoshihiro Ashihara ,  Yasushi Kasahara

    发明人： Yoshihiro Ashihara ,  Yasushi Kasahara

    IPC分类号： G01N33/535 ,  G01N33/542 ,  G01N33/573 ,  G01N33/58 ,  G01N33/74 ,  C12Q1/34 ,  C12Q1/40 ,  G01N33/536

    CPC分类号： G01N33/542 ,  G01N33/535 ,  G01N33/573 ,  G01N33/581 ,  G01N33/74 ,  Y10S435/963 ,  Y10S435/97 ,  Y10S436/813 ,  Y10S436/815

    摘要： A method is provided which is highly sensitive and specific, and is suitable as a clinical test for the determination of physiological substances and trace components in humoral fluid. The method employs a conjugate of an enzyme capable of acting on a water-insoluble macromolecular substance and an antibody against the ligand (1) to be measured or a ligand (2) having an antigenic determinant common to one of the antigenic determinant(s) of the ligand (1). In the method of the invention, steric hindrance of the conjugate is emphasized by the enzyme reaction which is carried out at the boundary between solid phase and liquid phase.

    摘要翻译： 提供了一种高灵敏度和特异性的方法，适用于测定体液中生理物质和微量成分的临床试验。 该方法使用能够作用于水不溶性大分子物质的酶和针对要测量的配体（1）的抗体的缀合物或具有抗原决定簇之一共有的抗原决定簇的配体（2） 的配体（1）。 在本发明的方法中，通过在固相和液相之间的边界处进行的酶反应来强调缀合物的空间位阻。

    Method for determining the activity of cholinesterases
    9.
    发明授权
    Method for determining the activity of cholinesterases 失效
    确定胆碱酯酶活性的方法

    公开(公告)号：US4419445A

    公开(公告)日：1983-12-06

    申请号：US360723

    申请日：1982-03-22

    申请人： Yasushi Kasahara ,  Yoshihiro Ashihara ,  Masami Sugiyama ,  Takahiro Harada

    发明人： Yasushi Kasahara ,  Yoshihiro Ashihara ,  Masami Sugiyama ,  Takahiro Harada

    IPC分类号： G01N33/50 ,  C12Q1/46 ,  C12Q1/26

    CPC分类号： C12Q1/46 ,  C12Q2334/00

    摘要： A method for determining the activity of cholinesterase comprising the steps of:mixing a solution containing p-methoxybenzoate demethylmonooxygenase, nicotinamide adenine dinucleotide reduced form (NADH) and p-methoxybenzoyl choline cation having the following formula: ##STR1## with a liquid containing cholinesterase, measuring the decrease in absorbance of light by NADH, which is based on a consumption of NADH, and calculating the activity of cholinesterase therefrom.

    摘要翻译： 一种确定胆碱酯酶活性的方法，包括以下步骤：将含有对甲氧基苯甲酸甲酯脱甲基单加氧酶，烟酰胺腺嘌呤二核苷酸还原形式（NADH）和具有下式的对甲氧基苯甲酰胆碱阳离子的溶液与含有胆碱酯酶的液体混合， 测量基于NADH消耗的NADH对光的吸光度的降低，并计算其中胆碱酯酶的活性。