公开(公告)号：US06645766B2

公开(公告)日：2003-11-11

申请号：US10043074

申请日：2002-01-07

申请人： Ying Luo ,  Pei Wen Yu ,  James Lorens

发明人： Ying Luo ,  Pei Wen Yu ,  James Lorens

IPC分类号： C12N1564

CPC分类号： C12N15/1082 ,  C12N15/1034 ,  C12N15/70 ,  C12N15/81

摘要： The invention provides shuttle vectors, and methods of using shuttle vectors, capable of expression in, at least, a mammalian cell. Furthermore, the shuttle vectors are capable of replication in at least yeast, and optionally, bacterial cells. Also provided is a method wherein yeast are transformed with a shuttle vector as provided herein. Heterologous nucleic acids flanked by 5′ and 3′ ends identical to a homologous recombination site within the shuttle vector are introduced to the transformed yeast and allowed to homologously recombine with the shuttle vector such that they are inserted into the vector by the yeast organism. The shuttle vector is then recovered and transferred to a mammalian cell for expression.

公开(公告)号：US06391582B2

公开(公告)日：2002-05-21

申请号：US09208827

申请日：1998-12-09

申请人： Ying Luo ,  Pei Wen Yu ,  James Lorens

发明人： Ying Luo ,  Pei Wen Yu ,  James Lorens

IPC分类号： C12P2102

CPC分类号： C12N15/1082 ,  C12N15/1034 ,  C12N15/70 ,  C12N15/81

摘要： The invention provides shuttle vectors, and methods of using shuttle vectors, capable of expression in, at least, a mammalian cell. Furthermore, the shuttle vectors are capable of replication in at least yeast, and optionally, bacterial cells. Also provided is a method wherein yeast are transformed with a shuttle vector as provided herein. Heterologous nucleic acids flanked by 5′ and 3′ ends identical to a homologous recombination site within the shuttle vector are introduced to the transformed yeast and allowed to homologously recombine with the shuttle vector such that they are inserted into the vector by the yeast organism. The shuttle vector is then recovered and transferred to a mammalian cell for expression.

摘要翻译： 本发明提供穿梭载体，以及使用能够在至少哺乳动物细胞中表达的穿梭载体的方法。 此外，穿梭载体能够在至少酵母和任选的细菌细胞中复制。 还提供了一种方法，其中用本文提供的穿梭载体转化酵母。 将与穿梭载体内的同源重组位点相同的5'和3'末端的异源核酸引入转化的酵母中，并与穿梭载体同源重组，使得它们被酵母生物插入载体。 然后将穿梭载体回收并转移至哺乳动物细胞进行表达。

公开(公告)号：US06280937B1

公开(公告)日：2001-08-28

申请号：US09133944

申请日：1998-08-14

申请人： Ying Luo ,  Pei Wen Yu ,  James Lorens

发明人： Ying Luo ,  Pei Wen Yu ,  James Lorens

IPC分类号： C12Q168

CPC分类号： C12N15/1082 ,  C12N15/70 ,  C12N15/81

摘要： The invention provides shuttle vectors, and methods of using shuttle vectors, capable of expression in, at least, a mammalian cell. Furthermore, the shuttle vectors are capable of replication in at least yeast, and optionally, bacterial cells. Also provided is a method wherein yeast are transformed with a shuttle vector as provided herein. Heterologous nucleic acids flanked by 5′ and 3′ ends identical to a homologous recombination site within the shuttle vector are introduced to the transformed yeast and allowed to homologously recombine with the shuttle vector such that they are inserted into the vector by the yeast organism. The shuttle vector is then recovered and transferred to a mammalian cell for expression.

摘要翻译： 本发明提供穿梭载体，以及使用能够在至少哺乳动物细胞中表达的穿梭载体的方法。 此外，穿梭载体能够在至少酵母和任选的细菌细胞中复制。 还提供了一种方法，其中用本文提供的穿梭载体转化酵母。 将与穿梭载体内的同源重组位点相同的5'和3'末端的异源核酸引入转化的酵母中，并与穿梭载体同源重组，使得它们被酵母生物体插入载体中。 然后将穿梭载体回收并转移至哺乳动物细胞进行表达。

公开(公告)号：US07488805B2

公开(公告)日：2009-02-10

申请号：US11739267

申请日：2007-04-24

申请人： Ying Luo ,  Betty Huang ,  Mary Shen ,  Pei Wen Yu

发明人： Ying Luo ,  Betty Huang ,  Mary Shen ,  Pei Wen Yu

IPC分类号： C07K16/00

CPC分类号： C07K14/4747 ,  A61K38/00

摘要： The present invention is directed to novel apoptosis polypeptides such as the Apop1, Apop2, and Apop3 proteins and related molecules which are involved in modulating apoptosis and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention. Further provided by the present invention are method for identifying novel compositions which modulate the biological activity of Apop1, Apop2, and Apop3, and the use of such compositions in diagnosis and treatment of disease.

摘要翻译： 本发明涉及新的凋亡多肽，例如参与调节凋亡的Apop1，Apop2和Apop3蛋白和相关分子以及编码这些多肽的核酸分子。 本文还提供了包含那些核酸序列的载体和宿主细胞，包含与异源多肽序列融合的本发明的多肽的嵌合多肽分子，与本发明的多肽结合的抗体以及本发明的多肽的制备方法 发明。 本发明还提供了鉴定调节Apop1，Apop2和Apop3的生物学活性的新组合物的方法，以及这些组合物在疾病诊断和治疗中的用途。

公开(公告)号：US07223850B1

公开(公告)日：2007-05-29

申请号：US09762491

申请日：1999-08-06

申请人： Ying Luo ,  Betty Huang ,  Mary Shen ,  Pei Wen Yu

发明人： Ying Luo ,  Betty Huang ,  Mary Shen ,  Pei Wen Yu

IPC分类号： C12N15/11 ,  C07K14/00

CPC分类号： C12N9/12

摘要： The present invention is directed to novel apoptosis polypeptides such as the Apop1, and Apop3 proteins and related molecules which are involved in modulating apoptosis and to nucleic acid molecules encoding those polypeptides. Also provided here are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention. Further provided by the present invention are methods for identifying novel compositions which modulate the biological activity of Apop1, Apop2, and Apop3, and the use of such compositions in diagnosis and treatment of disease.

摘要翻译： 本发明涉及新的凋亡多肽，例如涉及调节凋亡的Apop1和Apop3蛋白和相关分子以及编码那些多肽的核酸分子。 此处还提供了包含这些核酸序列的载体和宿主细胞，包含与异源多肽序列融合的本发明多肽的嵌合多肽分子，与本发明的多肽结合的抗体以及本发明的多肽的制备方法 发明。 本发明进一步提供了用于鉴定调节Apop1，Apop2和Apop3的生物活性的新组合物的方法，以及这些组合物在诊断和治疗疾病中的用途。

公开(公告)号：US06623980B1

公开(公告)日：2003-09-23

申请号：US09255920

申请日：1999-02-23

申请人： Joseph Fisher ,  James Lorens ,  David Anderson ,  Ying Luo ,  Chao Bai (Betty) Huang ,  Mary Shen

发明人： Joseph Fisher ,  James Lorens ,  David Anderson ,  Ying Luo ,  Chao Bai (Betty) Huang ,  Mary Shen

IPC分类号： G01N33566

CPC分类号： C07K14/4703

摘要： The present invention is directed to novel exocytotic polypeptides, such as Exo1 and Exo2 polypeptides and related molecules, which have an inhibitory effect on exocytosis and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention. Further provided by the present invention are method for identifying novel compositions which mediate exocytotic polypeptide bioactivity, and the use of such compositions in diagnosis and treatment of disease.

摘要翻译： 本发明涉及新颖的胞吐多肽，例如Exo1和Exo2多肽及相关分子，其对胞吐作用具有抑制作用，以及编码这些多肽的核酸分子。 本文还提供了包含那些核酸序列的载体和宿主细胞，包含与异源多肽序列融合的本发明的多肽的嵌合多肽分子，与本发明的多肽结合的抗体以及本发明的多肽的制备方法 发明。 本发明还提供了鉴定介导胞吐多肽生物活性的新组合物的方法，以及这些组合物在疾病诊断和治疗中的应用。

公开(公告)号：US08737846B2

公开(公告)日：2014-05-27

申请号：US13361873

申请日：2012-01-30

申请人： Xuezhe Zheng ,  Ying Luo ,  Guoliang Li ,  Ashok V. Krishnamoorthy

发明人： Xuezhe Zheng ,  Ying Luo ,  Guoliang Li ,  Ashok V. Krishnamoorthy

IPC分类号： H04B10/12 ,  G02B6/26 ,  G02B5/18

CPC分类号： H04J14/02 ,  G02B6/29308 ,  H04B10/506 ,  H04B10/572

摘要： An optical source uses feedback to maintain a substantially fixed spacing between adjacent wavelengths in a set of wavelengths in a wavelength comb output by the optical source. In particular, a set of light sources in the optical source provide optical signals having the set of wavelengths. Moreover, the optical signals are output at diffraction angles of an optical device in the optical source (such as an echelle grating), and optical detectors in the optical source determine optical metrics associated with the optical signals. Furthermore, control logic in the optical source provides control signals to the set of light sources based on the determined optical metrics.

摘要翻译： 光源使用反馈来维持由光源输出的波长梳输出中的一组波长中的相邻波长之间的基本上固定的间隔。 特别地，光源中的一组光源提供具有该组波长的光信号。 此外，光信号以光源（例如梯形光栅）中的光学装置的衍射角输出，并且光源中的光学检测器确定与光信号相关联的光学度量。 此外，光源中的控制逻辑基于所确定的光学量度向光源组提供控制信号。

公开(公告)号：US08543354B2

公开(公告)日：2013-09-24

申请号：US12914969

申请日：2010-10-28

申请人： Ying Luo ,  Rebecca K. Gottlieb ,  Meena Ramachandran ,  Chia-Hung Chiu ,  Nandita Dangui-Patel ,  Michael Kremliovsky ,  Jefferson Rose

发明人： Ying Luo ,  Rebecca K. Gottlieb ,  Meena Ramachandran ,  Chia-Hung Chiu ,  Nandita Dangui-Patel ,  Michael Kremliovsky ,  Jefferson Rose

IPC分类号： G06F17/18

CPC分类号： G01N33/66 ,  A61B5/0017 ,  A61B5/002 ,  A61B5/14532 ,  A61B5/1486 ,  A61B5/1495 ,  A61B5/6849 ,  A61B5/7207

摘要： Disclosed are methods, apparatuses, etc. for glucose sensor signal stability analysis. In certain example embodiments, a series of samples of at least one sensor signal that is responsive to a blood glucose level of a patient may be obtained. Based at least partly on the series of samples, at least one metric may be determined to assess an underlying trend of a change in responsiveness of the at least one sensor signal to the blood glucose level of the patient over time. A reliability of the at least one sensor signal to respond to the blood glucose level of the patient may be assessed based at least partly on the at least one metric assessing an underlying trend. Other example embodiments are disclosed herein.

公开(公告)号：US20110320166A1

公开(公告)日：2011-12-29

申请号：US12914969

申请日：2010-10-28

申请人： Ying Luo ,  Rebecca K. Gottlieb ,  Meena Ramachandran ,  Chia-Hung Chiu ,  Nandita Dangui-Patel ,  Michael Kremliovsky ,  Jefferson Rose

发明人： Ying Luo ,  Rebecca K. Gottlieb ,  Meena Ramachandran ,  Chia-Hung Chiu ,  Nandita Dangui-Patel ,  Michael Kremliovsky ,  Jefferson Rose

IPC分类号： G06F19/00 ,  G06F17/18

CPC分类号： G01N33/66 ,  A61B5/0017 ,  A61B5/002 ,  A61B5/14532 ,  A61B5/1486 ,  A61B5/1495 ,  A61B5/6849 ,  A61B5/7207

摘要： Disclosed are methods, apparatuses, etc. for glucose sensor signal stability analysis. In certain example embodiments, a series of samples of at least one sensor signal that is responsive to a blood glucose level of a patient may be obtained. Based at least partly on the series of samples, at least one metric may be determined to assess an underlying trend of a change in responsiveness of the at least one sensor signal to the blood glucose level of the patient over time. A reliability of the at least one sensor signal to respond to the blood glucose level of the patient may be assessed based at least partly on the at least one metric assessing an underlying trend. Other example embodiments are disclosed herein.

摘要翻译： 公开了用于葡萄糖传感器信号稳定性分析的方法，装置等。 在某些示例性实施例中，可以获得响应于患者的血糖水平的至少一个传感器信号的一系列样本。 至少部分地基于一系列样本，可以确定至少一个度量以评估至少一个传感器信号对患者随着时间的血糖水平的响应性的变化的潜在趋势。 可以至少部分地基于评估潜在趋势的至少一个度量来评估至少一个传感器信号对患者的血糖水平的响应的可靠性。 本文公开了其它示例性实施例。

公开(公告)号：US20080108098A1

公开(公告)日：2008-05-08

申请号：US11867504

申请日：2007-10-04

申请人： Cindy Leo ,  Ying Luo ,  Xiang Xu ,  Simon Yu

发明人： Cindy Leo ,  Ying Luo ,  Xiang Xu ,  Simon Yu

IPC分类号： C12Q1/48

CPC分类号： C12N9/1205 ,  C12Q1/485 ,  G01N33/5041 ,  G01N2500/02

摘要： The present invention provides compositions and methods for modulating cell proliferation, survival, morphology, and migration. Nucleic acids encoding proteins, and proteins so encoded which are capable of modulating proliferation, survival, morphology, and migration in mammalian cells are provided. Compositions and methods for the treatment of disorders related to cell proliferation, survival, morphology and migration are also provided. Prophylactics and methods for the prevention of such disorders are also provided. Also provided are compositions are methods for diagnostic and prognostic determination of such disorders. Further provided are assays for the identification of bioactive agents capable of modulating proliferation, survival, morphology, and migration in mammalian cells.

摘要翻译： 本发明提供调节细胞增殖，存活，形态和迁移的组合物和方法。 提供了编码蛋白质的核酸和如此编码的能够调节哺乳动物细胞中增殖，存活，形态和迁移的蛋白质。 还提供了用于治疗与细胞增殖，存活，形态和迁移相关的病症的组合物和方法。 还提供了预防和预防这种疾病的方法。 还提供组合物是用于诊断和预测这些疾病的方法。 进一步提供了用于鉴定能够调节哺乳动物细胞中增殖，存活，形态和迁移的生物活性剂的测定。