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    Method for detecting nucleic acid mutation by detecting chemiluminiscence generated with by-product of complementary strand extension reaction
    2.
    发明授权
    Method for detecting nucleic acid mutation by detecting chemiluminiscence generated with by-product of complementary strand extension reaction 有权
    通过检测由互补链延伸反应的副产物产生的化学发光检测来检测核酸突变的方法

    公开(公告)号：US06750018B2

    公开(公告)日：2004-06-15

    申请号：US09945703

    申请日：2001-09-05

    申请人： Hideki Kambara ,  Guohua Zhou ,  Kazunori Okano ,  Masao Kamahori

    发明人： Hideki Kambara ,  Guohua Zhou ,  Kazunori Okano ,  Masao Kamahori

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6858 ,  C12Q2565/537 ,  C12Q2565/301 ,  C12Q2535/125

    摘要： To provide a method and an apparatus for detecting DNA mutation without using electrophoresis, the presence or absence of specific sequence is detected by a method comprising a step of serially hybridizing a 5-base-long to 8-base-long short oligomer 6, which is capable of being engaged in the complementary strand extension reaction, and a long oligomer 61, which is capable of hybridizing with a target DNA but incapable of being engaged in the complementary strand extension reaction, with the target DNA 63; a step of carrying out the complementary strand extension reaction 66 starting from the short primer using four kinds of nucleic acid substrates and polymerase; and a step of detecting photo-emission, in which pyrophosphate 68 produced as a reaction by-product of the complementary strand extension reaction is converted into ATP and the photo-emission reaction is carried out using an enzyme.

    摘要翻译： 为了提供用于在不使用电泳的情况下检测DNA突变的方法和装置，通过包括与5-碱基长至8-碱基长的短寡聚物6串联杂交的步骤的方法来检测特异性序列的存在或不存在，其中 能够进行互补链延伸反应，以及能够与靶DNA杂交但不能与互补链延伸反应进行杂交的长寡聚体61与目标DNA 63反应; 使用四种核酸底物和聚合酶从短引物开始进行互补链延伸反应66的步骤; 检测发光的步骤，其中作为互补链延伸反应的反应副产物产生的焦磷酸盐68被转化为ATP，并且使用酶进行光发射反应。

    System and method for detecting biological and chemical material
    3.
    发明授权
    System and method for detecting biological and chemical material 有权
    检测生物和化学物质的系统和方法

    公开(公告)号：US07157050B2

    公开(公告)日：2007-01-02

    申请号：US10445038

    申请日：2003-05-27

    申请人： Yoshiaki Yazawa ,  Masao Kamahori ,  Hideki Kambara ,  Mitsuo Usami ,  Ken Takei

    发明人： Yoshiaki Yazawa ,  Masao Kamahori ,  Hideki Kambara ,  Mitsuo Usami ,  Ken Takei

    IPC分类号： G01N15/06

    CPC分类号： G01N33/54386 ,  G01N27/4145

    摘要： A system and method are provided for detecting biological and chemical material. To measure biological materials such as genes easily at low costs, the device for implementing a small-sized, high sensitive, economical measurement apparatus is provided. Probes appropriate for target biological materials are fixed on a chip, on which a sensor, identification number, and radio communication function are implemented, the captured targets are detected by the sensors, and the result of sensing are transferred to an external control unit by the radio communication function. The small-sized, high sensitivity measurement apparatus for detecting biological and chemical materials such as genes and measuring physical and chemical amounts such as temperature, pressure, pH, and the like can be implemented.

    摘要翻译： 提供了用于检测生物和化学材料的系统和方法。 为了容易地以低成本测量基因等生物体材料，提供了实现小型，高灵敏度，经济的测量装置的装置。 适用于目标生物材料的探针固定在其上实现传感器，识别号和无线电通信功能的芯片上，由传感器检测捕获的目标，并且通过传感器将感测结果传送到外部控制单元 无线电通信功能。 可以实现用于检测诸如基因的生物和化学材料以及测量物理和化学量如温度，压力，pH等的小尺寸，高灵敏度的测量装置。

    Luminescence detection apparatus
    8.
    发明申请
    Luminescence detection apparatus 审中-公开
    发光检测装置

    公开(公告)号：US20060141494A1

    公开(公告)日：2006-06-29

    申请号：US11209702

    申请日：2005-08-24

    申请人： Hideki Kambara ,  Tomoharu Kajiyama ,  Kunio Harada ,  Masao Kamahori

    发明人： Hideki Kambara ,  Tomoharu Kajiyama ,  Kunio Harada ,  Masao Kamahori

    IPC分类号： C12Q1/68 ,  C12M1/34

    CPC分类号： G01N21/03 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/76

    摘要： An object of the present invention is to provide a luminescence detection apparatus compact in size which is capable of conveniently determining DNA base sequences at a low cost. According to the present invention, a luminescence detection apparatus 1 is provided comprising: a plurality of reaction cells 6 each having a transparent bottom portion; a solution-dispensing portion 19 equipped with capillaries 18 positioned above the reaction cells 6 and put into a one-to-one correspondence with the reaction cells 6; and a light-detecting portion 29 having a plurality of light-sensing elements 24 put into a one-to-one correspondence with the reaction cells 6 and arranged in proximity to the bottom surfaces of the reaction cells 6, wherein the a plurality of light-sensing elements 24 of the light-detecting portion 29 detect respective luminescences in the reaction cells 6 generated by injecting reagent solutions from the solution-dispensing portion 19 to the reaction cells 6.

    摘要翻译： 本发明的目的是提供一种尺寸紧凑的发光检测装置，其能够以低成本方便地确定DNA碱基序列。 根据本发明，提供一种发光检测装置1，其包括：多个反应池6，每个反应池6具有透明底部; 溶液分配部分19，其配备有位于反应单元6上方并与反应单元6一一对应的毛细管18; 以及具有多个光检测元件24的光检测部分29，其与反应单元6一一对应地布置在反应单元6的底表面附近，其中多个光 光检测部分29的光敏元件24检测通过从溶液分配部分19将反应池6注入试剂溶液而产生的反应池6中的相应发光。

    Apparatus and method for measuring biological material
    9.
    发明申请
    Apparatus and method for measuring biological material 失效

    公开(公告)号：US20060016699A1

    公开(公告)日：2006-01-26

    申请号：US11072507

    申请日：2005-03-07

    申请人： Masao Kamahori ,  Yoshiaki Yazawa ,  Maki Shimoda

    发明人： Masao Kamahori ,  Yoshiaki Yazawa ,  Maki Shimoda

    IPC分类号： G01N27/26 ,  G01N33/487

    CPC分类号： G01N33/5438 ,  G01N27/4145

    摘要： Disclosed is a biomolecular detection device that can be used easily, at a low operating cost, and with a detection probe that can be immobilized easily. Using an insulated gate field effect transistor having a conductive electrode on the gate insulator between a source and a drain, a probe for detecting biomolecules is immobilized on the surface of the conductive electrode. For analysis, a conductive electrode on which a probe for detecting biomolecules is immobilized on the surface, and a reference electrode are placed in the sample solution in the analytical cell, an alternating current voltage is applied from a power source to the reference electrode and the electrical characteristics of the insulated gate field effect transistor that changes before and after binding of the measurement target substance such as DNA and proteins included in the sample solution with a probe for detecting biomolecules, namely the changes in the current values running between the source and the drain, are detected.

    Examination apparatus for biological sample and chemical sample
    10.
    发明授权
    Examination apparatus for biological sample and chemical sample 有权
    生物样品和化学样品检测仪器

    公开(公告)号：US07888708B2

    公开(公告)日：2011-02-15

    申请号：US11976831

    申请日：2007-10-29

    申请人： Yoshiaki Yazawa ,  Kazuki Watanabe ,  Masao Kamahori ,  Yukinori Kunimoto

    发明人： Yoshiaki Yazawa ,  Kazuki Watanabe ,  Masao Kamahori ,  Yukinori Kunimoto

    IPC分类号： H01L29/66

    CPC分类号： G01N27/414 ,  G01N27/4148 ,  H01L27/1203 ,  H01L51/0098

    摘要： A wireless sensor chip suitable for the compact, high-sensitive, and low-cost examination apparatus for easily examining a biological material such as gene at low cost is provided. A sensor chip is formed on an SOI substrate, and an n type semiconductor layer on which a pMOS transistor is formed and a p type semiconductor layer on which an nMOS transistor is formed are isolated by a pn junction. Therefore, the p type semiconductor layer at the outermost portion (chip edge portion to be in contact with solution) is set to floating, and the maximum potential and the minimum potential of the chip are supplied to an n type semiconductor layer and a p type semiconductor layer inside the outermost portion, respectively. Also, the chip is covered with an ion impermeable insulating film for reducing the penetration of positive ions through the oxide layer.

    摘要翻译： 提供了适用于以低成本容易地检测诸如基因等生物材料的紧凑，高灵敏度，低成本的检查装置的无线传感器芯片。 传感器芯片形成在SOI衬底上，并且其上形成有pMOS晶体管的n型半导体层和其上形成nMOS晶体管的p型半导体层被pn结隔离。 因此，最外侧部分（与溶液接触的芯片边缘部分）的p型半导体层被设置为浮置，并且芯片的最大电位和最小电位被提供给n型半导体层和p型半导体 分别在最外部分内。 此外，芯片被不透水的绝缘膜覆盖，用于减少正离子穿过氧化物层的穿透。