公开(公告)号：US5804142A

公开(公告)日：1998-09-08

申请号：US756430

申请日：1996-11-26

申请人： Masahito Ito ,  Junkichi Miura ,  Yoshio Fujii ,  Hiroshi Satake ,  Kasumi Yoshida

发明人： Masahito Ito ,  Junkichi Miura ,  Yoshio Fujii ,  Hiroshi Satake ,  Kasumi Yoshida

IPC分类号： G01N30/86 ,  G01N30/62 ,  G01N30/88 ,  G01N30/02

CPC分类号： G01N30/88 ,  G01N2030/626 ,  G01N2030/8804

摘要： A reference sample having a known concentration is injected into a chromatograph system before an unknown sample is analyzed. This is for the purpose of checking the deterioration of system performance or in other words for the purpose of checking system suitability. In the present invention, the chromatographic characteristic of the check sample at the time of deterioration of the system suitability is expected and stored in a memory in advance to be related to factors of deterioration of system suitability. A factor of deterioration is deduced by comparison between the actually analyzed chromatographic characteristic of the check sample and the relational data stored in the memory. A guidance required for removing the factor is displayed. Disclosed are preferred embodiments of a chromatograph system suitable for analyzing catecholamine or glycated hemoglobin.

摘要翻译： 在分析未知样品之前，将具有已知浓度的参考样品注入色谱系统中。 这是为了检查系统性能的恶化，换句话说是为了检查系统适用性。 在本发明中，期望在系统适用性恶化时检查样品的色谱特性，并预先存储在存储器中，以与系统适用性的劣化因素相关。 通过比较检查样品的实际分析色谱特征和存储在存储器中的关系数据，推导出劣化因素。 显示删除因子所需的指导。 公开了适用于分析儿茶酚胺或糖化血红蛋白的色谱系统的优选实施方案。

公开(公告)号：US5783450A

公开(公告)日：1998-07-21

申请号：US440359

申请日：1995-05-12

申请人： Kasumi Yoshida ,  Junkichi Miura ,  Yoshio Fujii ,  Hiroshi Satake ,  Masahito Ito ,  Masao Kamahori

发明人： Kasumi Yoshida ,  Junkichi Miura ,  Yoshio Fujii ,  Hiroshi Satake ,  Masahito Ito ,  Masao Kamahori

IPC分类号： G01N30/06 ,  G01N21/64 ,  G01N30/08 ,  G01N30/74 ,  G01N30/88 ,  G01N33/88 ,  G01N35/00 ,  G01N35/04 ,  G01N35/10 ,  G01N30/02

CPC分类号： G01N35/0092 ,  G01N30/88 ,  G01N2030/067 ,  G01N2030/8804 ,  G01N2030/8813 ,  G01N2035/00356 ,  G01N2035/0093 ,  G01N2035/106 ,  G01N21/64 ,  G01N30/06 ,  G01N35/028 ,  G01N35/109 ,  Y10T436/142222 ,  Y10T436/173845 ,  Y10T436/25375

摘要： A method and an instrument for separating and analyzing a very small quantity of constituents to be inspected contained in an analyte such as a class of catecholamines by liquid chromatography after labeling. The entire processes of analytical operations include principally three processes, a preparing process, an impurity elimination process and a separation process. When one sample is perceived, the sample is processed in these three processes. In this case, while one sample is being processed in an impurity elimination process, a sample preceeding the one sample is processed in a separation process, and a succeeding sample is processed in a preparing process. In other words, these three processes are performed essentially in parallel. With this, it is possible to remarkably reduce analysis time as compared with individual single sample analysis.

摘要翻译： 一种方法和仪器，用于通过液相色谱法在标记后通过液相色谱法分离和分析非常少量待检查的成分，包含在分析物如一类儿茶酚胺中。 分析操作的整个过程主要包括三个过程，制备过程，杂质消除过程和分离过程。 当一个样品被感知时，样品在这三个过程中被处理。 在这种情况下，当在杂质消除过程中处理一个样品时，在分离过程中处理先于一个样品的样品，并在制备过程中处理后续样品。 换句话说，这三个过程基本上是并行执行的。 与单个样品分析相比，可以显着减少分析时间。

公开(公告)号：US5308774A

公开(公告)日：1994-05-03

申请号：US625823

申请日：1990-12-11

申请人： Junkichi Miura ,  Masahito Ito ,  Yoshio Fujii ,  Hiroshi Satake ,  Kasumi Yoshida

发明人： Junkichi Miura ,  Masahito Ito ,  Yoshio Fujii ,  Hiroshi Satake ,  Kasumi Yoshida

IPC分类号： G01N30/06 ,  G01N21/64 ,  G01N30/04 ,  G01N30/14 ,  G01N30/24 ,  G01N30/74 ,  G01N30/88 ,  G01N35/00 ,  G01N35/04 ,  G01N35/10 ,  G01N33/88

CPC分类号： G01N30/24 ,  G01N35/00 ,  G01N2030/8804 ,  G01N2030/8813 ,  G01N2035/00356 ,  G01N2035/00435 ,  G01N2035/1032 ,  G01N21/64 ,  G01N30/74 ,  G01N35/028 ,  G01N35/1004 ,  G01N35/109 ,  Y10T436/173845 ,  Y10T436/25125 ,  Y10T436/25625

摘要： Physiologically active substances contained in a biological sample not subjected to deproteinization can be converted to their derivatives at a high efficiency without being adversely affected by the protein present in the sample, and accordingly the analysis of said physiologically active substances in the form of said derivatives can be performed at a high precision.

摘要翻译： 未经脱蛋白化的生物样品中含有的生理活性物质可以高效率地转化为其衍生物，而不受样品中存在的蛋白质的不利影响，因此所述衍生物形式的所述生理活性物质的分析可以 以高精度执行。

公开(公告)号：US5277871A

公开(公告)日：1994-01-11

申请号：US5310

申请日：1993-01-19

申请人： Yoshio Fujii ,  Kasumi Yoshida ,  Junkichi Miura ,  Hiroshi Satake ,  Masahito Ito

发明人： Yoshio Fujii ,  Kasumi Yoshida ,  Junkichi Miura ,  Hiroshi Satake ,  Masahito Ito

IPC分类号： G01N21/64 ,  G01N30/06 ,  G01N30/88 ,  G01N35/00 ,  G01N35/10 ,  B01D15/08

CPC分类号： G01N35/0092 ,  G01N30/88 ,  G01N2030/8804 ,  G01N2030/8813 ,  G01N2035/00356 ,  G01N2035/00435 ,  G01N2035/0093 ,  G01N2035/106 ,  G01N21/64 ,  G01N30/06 ,  G01N35/1004 ,  G01N35/109 ,  Y10T436/25625 ,  Y10T436/2575

摘要： The present invention provides a liquid chromatographic analyzer for analyzing a sample containing components for analysis by reacting the sample with a pre-labeling reagent to obtain a reaction mixture containing labeled components, concentrating the reaction mixture and separating the labeled components from each other, and detecting the separated labeled components by a detector, the steps of analysis being controlled to concurrently proceed by a control unit, thereby shortening the time for analysis per sample which analyzer includes: (a) a unit for storing samples containing components for analysis and a pre-labeling reagent for the components at a constant temperature, (b) a unit for reacting the sample with the pre-labeling reagent at a constant temperature to obtain a reaction mixture containing labeled components, (c) a unit for transferring one of the samples and the pre-labeling reagent from unit (a) to unit (b), (d) a unit for concentrating the reaction mixture and separating the labeled components from each other, (e) a unit for detecting the separated labeled components, and (f) a unit for controlling the steps of analysis to concurrently proceed, thereby shortening the time for analysis per sample.

摘要翻译： 本发明提供一种液相色谱分析仪，用于通过使样品与预标记试剂反应来分析含有分析成分的样品，以获得含有标记组分的反应混合物，浓缩反应混合物并将标记的组分彼此分离，并检测 通过检测器分离的标记的成分，分析的步骤被控制以由控制单元同时进行，从而缩短每个样品的分析时间，该分析仪包括：（a）用于存储包含用于分析的组分的样品的单元， （b）用于使样品与预标记试剂在恒定温度下反应以获得含有标记组分的反应混合物的单元，（c）用于转移样品之一的单元和 单元（a）至单位（b）的预标记试剂，（d）用于浓缩反应混合物的单元和分离 （e）用于检测分离的标记成分的单元，和（f）用于控制分析步骤同时进行的单元，从而缩短每个样品的分析时间。

公开(公告)号：US5670379A

公开(公告)日：1997-09-23

申请号：US372708

申请日：1995-01-13

申请人： Masahito Ito ,  Junkichi Miura ,  Yoshio Fujii ,  Hiroshi Satake ,  Mitsuo Ito ,  Fuminori Umesato, deceased

发明人： Masahito Ito ,  Junkichi Miura ,  Yoshio Fujii ,  Hiroshi Satake ,  Mitsuo Ito ,  Fuminori Umesato, deceased

IPC分类号： G01N30/86 ,  G01N30/02 ,  G01N30/62 ,  G01N30/82 ,  G01N30/88

CPC分类号： G01N30/82 ,  G01N30/88 ,  G01N2030/027 ,  G01N2030/625 ,  G01N2030/626 ,  G01N2030/8804 ,  G01N30/8631

摘要： A chromatograph system with improved automation is provided. In the chromatograph system of one embodiment, a regression line is set between retention times of predetermined peaks measured at each run in the past for a standard sample having known components. Referring to this regression line, the peak identifying condition, i.e., time window, is corrected. In another embodiment, there is provided a chromatograph apparatus having a function of estimating a limited life time of a system component. The analyzed results of the chromatograph system can thus be improved.

摘要翻译： 提供了自动化程度提高的色谱系统。 在一个实施方案的色谱系统中，对于具有已知组分的标准样品，在过去每次运行时测量的预定峰的保留时间之间设置回归线。 参考该回归线，校正峰识别条件，即时间窗。 在另一个实施方案中，提供了具有估计系统组分的有限寿命的功能的色谱仪。 因此可以提高色谱系统的分析结果。

公开(公告)号：US5436166A

公开(公告)日：1995-07-25

申请号：US180472

申请日：1994-01-12

申请人： Masahito Ito ,  Junkichi Miura ,  Yoshio Fujii ,  Hiroshi Satake ,  Fuzio Yamada ,  Kouichi Tagami ,  Fuminori Umesato

发明人： Masahito Ito ,  Junkichi Miura ,  Yoshio Fujii ,  Hiroshi Satake ,  Fuzio Yamada ,  Kouichi Tagami ,  Fuminori Umesato

IPC分类号： G01N30/86 ,  G01N1/00 ,  G01N21/64 ,  G01N30/24 ,  G01N30/74 ,  G01N30/88 ,  G01N35/10 ,  B01D15/08

CPC分类号： G01N35/1097 ,  G01N2030/047 ,  G01N2030/8804 ,  G01N21/64 ,  G01N30/24 ,  G01N30/74 ,  G01N30/8631 ,  G01N30/8658 ,  G01N30/8668 ,  G01N35/109

摘要： The present invention relates to a chromatography analysis method and a system employing the same which are capable of automatically determining an constituents to be detected contained in the unknown sample based on results on measuring a standard sample. The standard sample is first separated to obtain its chromatogram. The system extracts the necessary information from the chromatogram to identify the peaks corresponding to the constituents to be detected to set widths of time windows for the peaks. Then, the time windows thus set are applied to the chromatograms obtained by the separation of the unknown sample to identify the constituents contained in the unknown sample. By employing the present invention, it is unnecessary for an operator to input the retention time of each constituent to the system.

摘要翻译： 本发明涉及一种色谱分析方法及其采用该方法的系统，其基于测量标准样品的结果能够自动确定未知样品中包含的待检测成分。 首先分离标准样品以获得其色谱图。 系统从色谱图中提取必要的信息，以识别与要检测的成分相对应的峰以设置峰的时间窗宽度。 然后，将如此设置的时间窗应用于通过分离未知样品获得的色谱图，以鉴定未知样品中所含的成分。 通过采用本发明，操作者不必将每个成分的保留时间输入到系统。

公开(公告)号：US5827426A

公开(公告)日：1998-10-27

申请号：US711789

申请日：1996-09-10

申请人： Yoshio Fujii ,  Masahito Ito ,  Hiroshi Satake

发明人： Yoshio Fujii ,  Masahito Ito ,  Hiroshi Satake

IPC分类号： G01N33/68 ,  B01J20/281 ,  G01N30/02 ,  G01N30/52 ,  G01N30/60 ,  G01N30/84 ,  G01N30/88 ,  G01N30/96 ,  B01D15/08

CPC分类号： G01N30/96 ,  G01N2030/524 ,  G01N2030/8435 ,  G01N30/6052

摘要： A liquid chromatograph and chromatography which can perform analysis at a higher speed with clearer separation between peaks. The liquid chromatograph comprises a separation column (10), a buffer pump (7) for feeding a plurality of buffers 1, 2, 3 and 4 into the separation column (10), a sampler (9) disposed in a flow path between the buffer pump (7) and the separation column (10) for introducing a sample to be analyzed into the flow path, and a reaction coil (14) for mixing and then reacting amino acids separated by the separation column (10) with a ninhydrin reagent. The separation column is sized so that a ratio (L1/R) of its length L1 to its inner diameter R is not more than 10. The particle size of an ion exchange resin filled in the separation column (10) is selected to be not more than 4 .mu.m.

摘要翻译： 可以更高速度进行分析的液相色谱和色谱，峰间更清晰。 液相色谱仪包括分离塔（10），用于将多个缓冲液1,2,3和4供给到分离塔（10）中的缓冲泵（7），设置在流化床 缓冲泵（7）和用于将待分析样品引入流路的分离塔（10）和用于混合然后使由分离塔（10）分离的氨基酸与茚三酮试剂反应的反应线圈（14） 。 分离柱的尺寸使得其长度L1与其内径R的比（L1 / R）不大于10.填充在分离塔（10）中的离子交换树脂的粒径选择为不 超过4亩。

公开(公告)号：US06962809B1

公开(公告)日：2005-11-08

申请号：US10482626

申请日：2002-07-01

申请人： Kisaburo Deguchi ,  Genzo Hirata ,  Junkichi Miura ,  Masahito Ito ,  Shinichiro Nishimura ,  Susumu Nishiguchi ,  Atsushi Toda ,  Hiroaki Nakagawa ,  Kuriko Yamada

发明人： Kisaburo Deguchi ,  Genzo Hirata ,  Junkichi Miura ,  Masahito Ito ,  Shinichiro Nishimura ,  Susumu Nishiguchi ,  Atsushi Toda ,  Hiroaki Nakagawa ,  Kuriko Yamada

IPC分类号： C12P19/14 ,  C12P19/18 ,  C12M1/34

CPC分类号： C12P19/18 ,  C12P19/14

摘要： A sugar chain synthesizer comprising one or more reaction columns packed with immobilized glycosyltransferase and/or glycosidase; one or more separation means, arranged downstream from the reaction columns, for separating reaction products, unreaction products and byproducts contained in the solution eluted from the reaction columns; a first pump for feeding a primer of water soluble polymer and buffer solution to the reaction columns through a first selector valve; a second pump for feeding a buffer solution and sugar nucleotide solution to any one of the reaction columns through a second selector valve; one or more circulation flow paths connecting between a flow path downstream from the separation means and a flow path upstream from each of the reaction columns; and a third selector valve, arranged between the separation means and one or more circulation flow paths, for selective connection between the separation means and a desired circulation flow path. This synthesizer ensures continuous and automatic synthesis of sugar chains even if complicated.

摘要翻译： 一种糖链合成仪，其包含一个或多个填充有固定的糖基转移酶和/或糖苷酶的反应柱; 一个或多个分离装置，布置在反应塔的下游，用于分离从反应塔洗脱的溶液中所含的反应产物，未反应产物和副产物; 第一泵，用于通过第一选择阀向反应塔供给水溶性聚合物和缓冲溶液的底漆; 第二泵，其通过第二选择阀将缓冲溶液和糖核苷酸溶液供给到任一反应塔; 连接在分离装置下游的流动路径和每个反应塔上游的流动路径之间的一个或多个循环流动路径; 以及第三选择阀，布置在所述分离装置和一个或多个循环流动路径之间，用于分离装置和期望的循环流动路径之间的选择性连接。 这种合成器即使复杂，也能确保糖链的连续和自动合成。

公开(公告)号：US20070199874A1

公开(公告)日：2007-08-30

申请号：US11700844

申请日：2007-02-01

申请人： Masahito Ito ,  Junkichi Miura ,  Kisaburo Deguchi

发明人： Masahito Ito ,  Junkichi Miura ,  Kisaburo Deguchi

IPC分类号： B01D15/08

CPC分类号： G01N30/463 ,  G01N30/461 ,  G01N30/7233 ,  G01N30/96 ,  B01D15/322 ,  B01D15/362 ,  B01D15/325 ,  B01D15/363

摘要： In a liquid chromatography apparatus, a separation column of intermediate stage is additionally connected between a separation column of first stage and a separation column of second stage. Preferably, a switching unit and a liquid feed unit for mixing and feeding a plurality of solutions are added to improve a separation capability. A three-dimensional liquid chromatography apparatus capable of avoiding the “solution interference” can be realized. Even a complex sample containing a hydrophilic component and a hydrophobic component in a mixed state can be separated and analyzed satisfactorily on-line.

摘要翻译： 在液相色谱装置中，中间级分离塔另外连接在第一级分离塔和第二级分离塔之间。 优选地，添加用于混合和供给多个溶液的切换单元和液体进料单元以提高分离能力。 可以实现能够避免“溶液干扰”的三维液相色谱仪。 即使是含有亲水性成分和混合状态的疏水性成分的复合物，也可以在线进行分离和分析。

公开(公告)号：US5407568A

公开(公告)日：1995-04-18

申请号：US118704

申请日：1993-09-09

申请人： Junkichi Miura ,  Kenji Yasuda ,  Mitsuo Ito ,  Masahito Ito

发明人： Junkichi Miura ,  Kenji Yasuda ,  Mitsuo Ito ,  Masahito Ito

IPC分类号： G01N30/02 ,  B01D15/08 ,  B01J39/26 ,  G01N30/50 ,  G01N30/96

CPC分类号： G01N30/96 ,  B01D15/20 ,  B01D15/363 ,  B01J39/26

摘要： Deterioration of performance of a separation column for liquid chromatograph due to changes in the functional groups during the preservation or application is prevented by filling a solution containing a component in the molecule with the same structure as that of the chemical function group of a packing material for the separation column as a filling liquid together with the packing material in the separation column, and also by washing the separation column with the same filling liquid as a washing solution after the analysis and retaining the washing solution therein, whereby the preservation stability of the separation column is improved and the separation column can be preserved at room temperature.

摘要翻译： 通过将包含分子中的成分的溶液与包装材料的化学官能团相同的结构填充来防止由于保存或施加期间官能团的变化而导致的液相色谱分离柱的性能劣化 分离塔作为填充液与分离塔中的包装材料一起，并且在分析后保持与洗涤液相同的填充液，同时通过洗涤分离塔，从而保持分离的保存稳定性 柱被改善，分离柱可以在室温下保存。